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$280 100ul
$350 200ul

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  • Product Name
    PSMB5 Antibody
  • Catalog No.
    DF6728
  • RRID
    AB_2838690
  • Source
    Rabbit
  • Application
    WB,IHC,ELISA(peptide)
  • Reactivity
    Human, Mouse, Rat
  • Prediction
    Bovine, Horse, Sheep, Rabbit, Dog, Xenopus
  • UniProt
  • Mol.Wt
    28kD, 20 kD;
    28kDa(Calculated).
  • Concentration
    1mg/ml
  • Browse similar products>>

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Product Information

Alternative Names:Expand▼

DKFZp459C139; EC 3.4.25.1; LMPX; Macropain epsilon chain; MB1; MGC104214; MGC118075; MGC134464; Multicatalytic endopeptidase complex epsilon chain; Proteasome (prosome, macropain) subunit, beta type, 5; Proteasome beta 5 subunit; Proteasome catalytic subunit 3; Proteasome chain 6; Proteasome epsilon chain; Proteasome subunit beta type-5; Proteasome subunit MB1; Proteasome subunit X; Proteasome subunit, beta type, 5; Proteasome subunit, beta-5; PSB5_HUMAN; PSMB5; PSX large multifunctional protease X; X;

Applications:

WB 1:500-1:2000, IHC 1:50-1:200, ELISA(peptide) 1:20000-1:40000
*The optimal dilutions should be determined by the end user.

Reactivity:

Human, Mouse, Rat

Predicted Reactivity:

Bovine, Horse, Sheep, Rabbit, Dog, Xenopus

Source:

Rabbit

Clonality:

Polyclonal

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Specificity:

PSMB5 Antibody detects endogenous levels of total PSMB5.

RRID:

AB_2838690
Please cite this product as: Affinity Biosciences Cat# DF6728, RRID:AB_2838690.

Format:

Liquid

Concentration:

1mg/ml

Storage Condition and Buffer:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt.

Immunogen Information in 3D

Immunogen:

A synthesized peptide derived from human PSMB5, corresponding to a region within C-terminal amino acids.

Uniprot:



>>Visit The Human Protein Atlas

Gene ID:

Gene Name:

PSMB5

Molecular Weight:

Observed Mol.Wt.: 28kD, 20 kD.
Predicted Mol.Wt.: 28kDa(Calculated)..

Subcellular Location:

Cytoplasm. Nucleus.

Description:

The proteasome is a multicatalytic proteinase complex with a highly ordered ring-shaped 20S core structure. The core structure is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. This gene encodes a member of the proteasome B-type family, also known as the T1B family, that is a 20S core beta subunit in the proteasome. This catalytic subunit is not present in the immunoproteasome and is replaced by catalytic subunit 3i (proteasome beta 8 subunit). Multiple transcript variants encoding different isoforms have been found for this gene.

Sequence:
MALASVLERPLPVNQRGFFGLGGRADLLDLGPGSLSDGLSLAAPGWGVPEEPGIEMLHGTTTLAFKFRHGVIVAADSRATAGAYIASQTVKKVIEINPYLLGTMAGGAADCSFWERLLARQCRIYELRNKERISVAAASKLLANMVYQYKGMGLSMGTMICGWDKRGPGLYYVDSEGNRISGATFSVGSGSVYAYGVMDRGYSYDLEVEQAYDLARRAIYQATYRDAYSGGAVNLYHVREDGWIRVSSDNVADLHEKYSGSTP

Research Background

Function:

Component of the 20S core proteasome complex involved in the proteolytic degradation of most intracellular proteins. This complex plays numerous essential roles within the cell by associating with different regulatory particles. Associated with two 19S regulatory particles, forms the 26S proteasome and thus participates in the ATP-dependent degradation of ubiquitinated proteins. The 26S proteasome plays a key role in the maintenance of protein homeostasis by removing misfolded or damaged proteins that could impair cellular functions, and by removing proteins whose functions are no longer required. Associated with the PA200 or PA28, the 20S proteasome mediates ubiquitin-independent protein degradation. This type of proteolysis is required in several pathways including spermatogenesis (20S-PA200 complex) or generation of a subset of MHC class I-presented antigenic peptides (20S-PA28 complex). Within the 20S core complex, PSMB5 displays a chymotrypsin-like activity.

Subcellular Location:

Cytoplasm. Nucleus.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte

Subunit Structure:

The 26S proteasome consists of a 20S proteasome core and two 19S regulatory subunits. The 20S proteasome core is a barrel-shaped complex made of 28 subunits that are arranged in four stacked rings. The two outer rings are each formed by seven alpha subunits, and the two inner rings are formed by seven beta subunits. The proteolytic activity is exerted by three beta-subunits PSMB5, PSMB6 and PSMB7. Directly interacts with POMP. Interacts with ABCB1 and TAP1.

(Microbial infection) Interacts with HIV-1 TAT protein.

Similarity:

Belongs to the peptidase T1B family.

Research Fields

Research Fields:

· Genetic Information Processing > Folding, sorting and degradation > Proteasome.

Reference Citations:

1). Guo S;Chen Y;Yang Y;Zhang X;Ma L;Xue X;Qiao Y;Wang J; et al. TRIB2 modulates proteasome function to reduce ubiquitin stability and protect liver cancer cells against oxidative stress. Cell Death Dis 2021 Jan 7;12(1):42. (PubMed: 33414446) [IF=6.304]

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Catalog Number :

DF6728-BP
(Blocking peptide available as DF6728-BP)

Price/Size :

$350/1mg.
Tips: For modified antibodies, we provide modified peptides(0.5mg) and non-modified peptides(0.5mg).

Function :

Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. This mechanism is useful when non-specific binding is an issue, for example, in Western blotting (immunoblot) and immunohistochemistry (IHC). By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific; Specific binding will be absent from the western blot or immunostaining performed with the neutralized antibody.

Format and storage :

Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 10 mg/ml.The purity is >90%,tested by HPLC and MS.Storage Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C.

Precautions :

This product is for research use only. Not for use in diagnostic or therapeutic procedures.

Horse
100%
Bovine
100%
Sheep
100%
Dog
100%
Rabbit
100%
Xenopus
92%
Chicken
67%
Zebrafish
58%
Pig
0%
High similarity Medium similarity Low similarity No similarity
P28074 as Substrate
Site PTM Type Enzyme
S87 Phosphorylation
K91 Ubiquitination
K92 Ubiquitination
Y99 Phosphorylation
S139 Phosphorylation
K140 Ubiquitination
Y147 Phosphorylation
Y149 Phosphorylation
K165 Ubiquitination
Y171 Phosphorylation
S175 Phosphorylation
Y212 Phosphorylation
Y220 Phosphorylation
Y228 Phosphorylation
Y236 Phosphorylation
S247 Phosphorylation
S248 Phosphorylation
K257 Acetylation
K257 Ubiquitination
S261 Phosphorylation
T262 Phosphorylation
IMPORTANT: For western blots, incubate membrane with diluted antibody in 5% w/v milk , 1X TBS, 0.1% Tween®20 at 4°C with gentle shaking, overnight.

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