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$280 100ul
$350 200ul

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  • Product Name
    PSMD2 Antibody
  • Catalog No.
    DF6743
  • RRID
    AB_2838705
  • Source
    Rabbit
  • Application
    WB,IHC,IF/ICC,ELISA(peptide)
  • Reactivity
    Human, Mouse, Rat
  • Prediction
    Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus
  • UniProt
  • Mol.Wt
    100kD;
    100kDa(Calculated).
  • Concentration
    1mg/ml
  • Browse similar products>>

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Product Information

Alternative Names:Expand▼

26S proteasome non-ATPase regulatory subunit 2; 26S proteasome regulatory subunit RPN1; 26S proteasome regulatory subunit S2; 26S proteasome subunit p97; 55.11 protein; MGC14274; P97; Proteasome (prosome macropain) 26S subunit non ATPase 2; Proteasome 26S subunit, non-ATPase, 2; Proteasome 26S, subunit 2; Protein 55.11; Psmd2; PSMD2_HUMAN; Rpn1; S2; TNFR associated protein 2; TRAP2; Tumor necrosis factor receptor associated protein 2; Tumor necrosis factor type 1 receptor-associated protein 2;

Applications:

WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500, ELISA(peptide) 1:20000-1:40000
*The optimal dilutions should be determined by the end user.

Reactivity:

Human, Mouse, Rat

Predicted Reactivity:

Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus

Source:

Rabbit

Clonality:

Polyclonal

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Specificity:

PSMD2 Antibody detects endogenous levels of total PSMD2.

RRID:

AB_2838705
Please cite this product as: Affinity Biosciences Cat# DF6743, RRID:AB_2838705.

Format:

Liquid

Concentration:

1mg/ml

Storage Condition and Buffer:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt.

Immunogen Information in 3D

Immunogen:

A synthesized peptide derived from human PSMD2, corresponding to a region within C-terminal amino acids.

Uniprot:



>>Visit The Human Protein Atlas

Gene ID:

Gene Name:

PSMD2

Molecular Weight:

Observed Mol.Wt.: 100kD.
Predicted Mol.Wt.: 100kDa(Calculated)..

Tissue Specificity:

Q13200 PSMD2_HUMAN:
Found in skeletal muscle, liver, heart, brain, kidney, pancreas, lung and placenta.

Description:

The 26S proteasome is a multicatalytic proteinase complex with a highly ordered structure composed of 2 complexes, a 20S core and a 19S regulator. The 20S core is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. The 19S regulator is composed of a base, which contains 6 ATPase subunits and 2 non-ATPase subunits, and a lid, which contains up to 10 non-ATPase subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. This gene encodes one of the non-ATPase subunits of the 19S regulator lid. In addition to participation in proteasome function, this subunit may also participate in the TNF signalling pathway since it interacts with the tumor necrosis factor type 1 receptor. A pseudogene has been identified on chromosome 1. Alternative splicing results in multiple transcript variants of this gene.

Sequence:
MEEGGRDKAPVQPQQSPAAAPGGTDEKPSGKERRDAGDKDKEQELSEEDKQLQDELEMLVERLGEKDTSLYRPALEELRRQIRSSTTSMTSVPKPLKFLRPHYGKLKEIYENMAPGENKRFAADIISVLAMTMSGERECLKYRLVGSQEELASWGHEYVRHLAGEVAKEWQELDDAEKVQREPLLTLVKEIVPYNMAHNAEHEACDLLMEIEQVDMLEKDIDENAYAKVCLYLTSCVNYVPEPENSALLRCALGVFRKFSRFPEALRLALMLNDMELVEDIFTSCKDVVVQKQMAFMLGRHGVFLELSEDVEEYEDLTEIMSNVQLNSNFLALARELDIMEPKVPDDIYKTHLENNRFGGSGSQVDSARMNLASSFVNGFVNAAFGQDKLLTDDGNKWLYKNKDHGMLSAAASLGMILLWDVDGGLTQIDKYLYSSEDYIKSGALLACGIVNSGVRNECDPALALLSDYVLHNSNTMRLGSIFGLGLAYAGSNREDVLTLLLPVMGDSKSSMEVAGVTALACGMIAVGSCNGDVTSTILQTIMEKSETELKDTYARWLPLGLGLNHLGKGEAIEAILAALEVVSEPFRSFANTLVDVCAYAGSGNVLKVQQLLHICSEHFDSKEKEEDKDKKEKKDKDKKEAPADMGAHQGVAVLGIALIAMGEEIGAEMALRTFGHLLRYGEPTLRRAVPLALALISVSNPRLNILDTLSKFSHDADPEVSYNSIFAMGMVGSGTNNARLAAMLRQLAQYHAKDPNNLFMVRLAQGLTHLGKGTLTLCPYHSDRQLMSQVAVAGLLTVLVSFLDVRNIILGKSHYVLYGLVAAMQPRMLVTFDEELRPLPVSVRVGQAVDVVGQAGKPKTITGFQTHTTPVLLAHGERAELATEEFLPVTPILEGFVILRKNPNYDL

Research Background

Function:

Component of the 26S proteasome, a multiprotein complex involved in the ATP-dependent degradation of ubiquitinated proteins. This complex plays a key role in the maintenance of protein homeostasis by removing misfolded or damaged proteins, which could impair cellular functions, and by removing proteins whose functions are no longer required. Therefore, the proteasome participates in numerous cellular processes, including cell cycle progression, apoptosis, or DNA damage repair.

Binds to the intracellular domain of tumor necrosis factor type 1 receptor. The binding domain of TRAP1 and TRAP2 resides outside the death domain of TNFR1.

Tissue Specificity:

Found in skeletal muscle, liver, heart, brain, kidney, pancreas, lung and placenta.

Subunit Structure:

Component of the 19S proteasome regulatory particle complex. The 26S proteasome consists of a 20S core particle (CP) and two 19S regulatory subunits (RP). The regulatory particle is made of a lid composed of 9 subunits, a base containing 6 ATPases and few additional components including PSMD2. Interacts with RPGRIP1L (By similarity). Interacts with CRY1 in a KDM8-dependent manner (By similarity).

Similarity:

Belongs to the proteasome subunit S2 family.

Research Fields

Research Fields:

· Genetic Information Processing > Folding, sorting and degradation > Proteasome.
· Human Diseases > Infectious diseases: Viral > Epstein-Barr virus infection.

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Catalog Number :

DF6743-BP
(Blocking peptide available as DF6743-BP)

Price/Size :

$350/1mg.
Tips: For modified antibodies, we provide modified peptides(0.5mg) and non-modified peptides(0.5mg).

Function :

Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. This mechanism is useful when non-specific binding is an issue, for example, in Western blotting (immunoblot) and immunohistochemistry (IHC). By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific; Specific binding will be absent from the western blot or immunostaining performed with the neutralized antibody.

Format and storage :

Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 10 mg/ml.The purity is >90%,tested by HPLC and MS.Storage Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C.

Precautions :

This product is for research use only. Not for use in diagnostic or therapeutic procedures.

Pig
100%
Horse
100%
Bovine
100%
Sheep
100%
Dog
100%
Zebrafish
100%
Chicken
100%
Rabbit
100%
Xenopus
89%
High similarity Medium similarity Low similarity No similarity
Q13200 as Substrate
Site PTM Type Enzyme
K8 Ubiquitination
S16 Phosphorylation
T24 Phosphorylation
K27 Ubiquitination
S29 Phosphorylation
K31 Ubiquitination
K39 Ubiquitination
K41 Ubiquitination
S46 Phosphorylation
K50 Ubiquitination
K66 Ubiquitination
R79 Methylation
K94 Ubiquitination
K97 Ubiquitination
K105 Ubiquitination
K107 Ubiquitination
Y110 Phosphorylation
K119 Ubiquitination
S134 Phosphorylation
R137 Methylation
K141 Ubiquitination
S147 Phosphorylation
Y158 Phosphorylation
K168 Ubiquitination
K178 Ubiquitination
T186 Phosphorylation
K189 Ubiquitination
Y194 Phosphorylation
Y226 Phosphorylation
K228 Ubiquitination
K286 Ubiquitination
K292 Ubiquitination
K343 Ubiquitination
Y349 Phosphorylation
K350 Acetylation
K350 Ubiquitination
R357 Methylation
S361 Phosphorylation
S363 Phosphorylation
K397 Ubiquitination
Y432 Phosphorylation
Y434 Phosphorylation
S435 Phosphorylation
S436 Phosphorylation
Y439 Phosphorylation
K441 Ubiquitination
S442 Phosphorylation
T499 Phosphorylation
S508 Phosphorylation
K551 Ubiquitination
Y554 Phosphorylation
K623 Ubiquitination
S711 Phosphorylation
K712 Ubiquitination
K754 Ubiquitination
K773 Ubiquitination
K858 Ubiquitination
K860 Ubiquitination
K902 Ubiquitination
Y906 Phosphorylation
IMPORTANT: For western blots, incubate membrane with diluted antibody in 5% w/v milk , 1X TBS, 0.1% Tween®20 at 4°C with gentle shaking, overnight.

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