PTP4A3 Antibody - #DF6747

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Product: PTP4A3 Antibody
Catalog: DF6747
Description: Rabbit polyclonal antibody to PTP4A3
Application: WB IHC IF/ICC
Reactivity: Human, Mouse, Rat
Prediction: Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus
Mol.Wt.: 15~26kD; 20kD(Calculated).
Uniprot: O75365
RRID: AB_2838709

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Lead Time: Same day delivery

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(91%), Bovine(91%), Horse(91%), Sheep(91%), Rabbit(82%), Dog(82%), Chicken(90%), Xenopus(90%)
Clonality:
Polyclonal
Specificity:
PTP4A3 Antibody detects endogenous levels of total PTP4A3.
RRID:
AB_2838709
Cite Format: Affinity Biosciences Cat# DF6747, RRID:AB_2838709.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

Potentially prenylated protein tyrosine phosphatase; PRL 3; PRL R; PRL-3; PRL-R; PRL3; PRLR; Protein tyrosine phosphatase 4a3; Protein tyrosine phosphatase type IVA 3; Protein-tyrosine phosphatase 4a3; Protein-tyrosine phosphatase of regenerating liver 3; PTP 4A3; PTP4A3; TP4A3_HUMAN;

Immunogens

Immunogen:
Uniprot:

O75365(TP4A3_HUMAN) >>Visit HPA database.

Gene(ID):
PTP4A3(11156)
Expression:
O75365 TP4A3_HUMAN:

Mainly expressed in cardiomyocytes and skeletal muscle; also found in pancreas. Consistently overexpressed in colon cancer metastasis.

Description:
This gene encodes a member of the protein-tyrosine phosphatase family. Protein tyrosine phosphatases are cell signaling molecules that play regulatory roles in a variety of cellular processes. Studies of this class of protein tyrosine phosphatase in mice demonstrates that they are prenylated in vivo, suggesting their association with cell plasma membrane. The encoded protein may enhance cell proliferation, and overexpression of this gene has been implicated in tumor metastasis. Alternative splicing results in multiple transcript variants.
Sequence:
MARMNRPAPVEVSYKHMRFLITHNPTNATLSTFIEDLKKYGATTVVRVCEVTYDKTPLEKDGITVVDWPFDDGAPPPGKVVEDWLSLVKAKFCEAPGSCVAVHCVAGLGRAPVLVALALIESGMKYEDAIQFIRQKRRGAINSKQLTYLEKYRPKQRLRFKDPHTHKTRCCVM

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
91
Horse
91
Bovine
91
Sheep
91
Xenopus
90
Chicken
90
Dog
82
Rabbit
82
Zebrafish
60
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - O75365 As Substrate

Site PTM Type Enzyme
S13 Phosphorylation
Y14 Phosphorylation
K155 Acetylation
K167 Acetylation

Research Backgrounds

Function:

Protein tyrosine phosphatase which stimulates progression from G1 into S phase during mitosis. Enhances cell proliferation, cell motility and invasive activity, and promotes cancer metastasis. May be involved in the progression of cardiac hypertrophy by inhibiting intracellular calcium mobilization in response to angiotensin II.

PTMs:

Farnesylated. Farnesylation is required for membrane targeting (By similarity).

Subcellular Location:

Cell membrane. Early endosome.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Mainly expressed in cardiomyocytes and skeletal muscle; also found in pancreas. Consistently overexpressed in colon cancer metastasis.

Subunit Structure:

Interacts with tubulin.

Family&Domains:

Belongs to the protein-tyrosine phosphatase family.

References

1). The phosphatase of regenerating liver-3 protein (PRL-3) promotes glioma cell invasiveness by interacting with β3–tubulin. Bioengineered (PubMed: 35098869) [IF=4.9]

Application: WB    Species: Human    Sample: U87MG cells

Figure 1. Western blot analysis of PRL-3 after overexpression and knockdown. The lentiviral vectors overexpressing PRL-3 (PRL-3 OE) or silencing PRL-3 (PRL-3 KD) were used to transduce the U87MG cells and the stably transfected cell lines were lysed for Western blot analysis. The empty vectors (OE-NC, KD-NC) were used as controls.

Restrictive clause

 

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For Research Use Only.
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