Product Info

Source:
Mouse
Application:
ELISA 1:10000, WB 1:500-1:2000, IHC 1:200-1:1000
*The optimal dilutions should be determined by the end user.
Reactivity:
Human
Clonality:
Monoclonal [AFB1790]
Specificity:
GATA3 antibody detects endogenous levels of total GATA3.
RRID:
AB_2833814
Cite Format: Affinity Biosciences Cat# BF0568, RRID:AB_2833814.
Purification:
Affinity-chromatography.
Storage:
Mouse IgG1 in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

GATA 3; GATA binding factor 3; GATA binding protein 3; GATA-binding factor 3; Gata3; GATA3_HUMAN; HDR; HDRS; MGC2346; MGC5199; MGC5445; Trans acting T cell specific transcription factor GATA 3; Trans-acting T-cell-specific transcription factor GATA-3;

Immunogens

Immunogen:

Purified recombinant fragment of human GATA3 expressed in E. Coli.

Uniprot:
Gene(ID):
Expression:
P23771 GATA3_HUMAN:

T-cells and endothelial cells.

Description:
GATA3 is a transcriptional activator which binds to the enhancer of the T cell receptor alpha and delta genes. Binds to the consensus sequence 5'-AGATAG-3'.
Sequence:
MEVTADQPRWVSHHHPAVLNGQHPDTHHPGLSHSYMDAAQYPLPEEVDVLFNIDGQGNHVPPYYGNSVRATVQRYPPTHHGSQVCRPPLLHGSLPWLDGGKALGSHHTASPWNLSPFSKTSIHHGSPGPLSVYPPASSSSLSGGHASPHLFTFPPTPPKDVSPDPSLSTPGSAGSARQDEKECLKYQVPLPDSMKLESSHSRGSMTALGGASSSTHHPITTYPPYVPEYSSGLFPPSSLLGGSPTGFGCKSRPKARSSTGRECVNCGATSTPLWRRDGTGHYLCNACGLYHKMNGQNRPLIKPKRRLSAARRAGTSCANCQTTTTTLWRRNANGDPVCNACGLYYKLHNINRPLTMKKEGIQTRNRKMSSKSKKCKKVHDSLEDFPKNSSFNPAALSRHMSSLSHISPFSHSSHMLTTPTPMHPPSSLSFGPHHPSSMVTAMG

PTMs - P23771 As Substrate

Site PTM Type Enzyme
Ubiquitination
S110 Phosphorylation
S115 Phosphorylation
T156 Phosphorylation P24941 (CDK2)
S162 Phosphorylation
S166 Phosphorylation
S172 Phosphorylation
Y186 Phosphorylation
K195 Ubiquitination
R261 Methylation
T271 Phosphorylation
Y282 Phosphorylation
Y290 Phosphorylation
K292 Acetylation
K304 Acetylation
S308 Phosphorylation P17612 (PRKACA)
T315 Phosphorylation
S316 Phosphorylation
Y344 Phosphorylation
K346 Acetylation
S369 Phosphorylation
K371 Acetylation
K373 Acetylation
K374 Acetylation
K376 Acetylation
K377 Ubiquitination
S381 Phosphorylation

Research Backgrounds

Function:

Transcriptional activator which binds to the enhancer of the T-cell receptor alpha and delta genes. Binds to the consensus sequence 5'-AGATAG-3'. Required for the T-helper 2 (Th2) differentiation process following immune and inflammatory responses.

Subcellular Location:

Nucleus.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

T-cells and endothelial cells.

Subunit Structure:

Interacts with TBX21 ('Tyr-530' phosphorylated form).

Family&Domains:

Binds DNA via the 2 GATA-type zinc fingers. Each zinc finger may bind either adjacent sites in a palindromic motif, or a different DNA molecule allowing looping and long-range gene regulation.

The YxKxHxxxRP motif is critical for DNA-binding and function.

Research Fields

· Human Diseases > Immune diseases > Inflammatory bowel disease (IBD).

· Organismal Systems > Immune system > Th1 and Th2 cell differentiation.   (View pathway)

· Organismal Systems > Immune system > Th17 cell differentiation.   (View pathway)

Restrictive clause

 

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