CD69 Antibody - #DF6777

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Product: CD69 Antibody
Catalog: DF6777
Description: Rabbit polyclonal antibody to CD69
Application: WB IHC IF/ICC
Reactivity: Human, Mouse, Rat
Prediction: Sheep, Dog
Mol.Wt.: 20kDa(Observed); 23kD(Calculated).
Uniprot: Q07108
RRID: AB_2838739

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 100ul $280 In stock
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Related Downloads
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user. For optimal experimental results, antibody reuse is not recommended.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Sheep(86%), Dog(88%)
Clonality:
Polyclonal
Specificity:
CD69 Antibody detects endogenous levels of total CD69.
RRID:
AB_2838739
Cite Format: Affinity Biosciences Cat# DF6777, RRID:AB_2838739.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

Activation inducer molecule (AIM/CD69); Activation inducer molecule; AIM; BL-AC/P26; BLAC/P26; C-type lectin domain family 2 member C; CD69; CD69 antigen (p60, early T-cell activation antigen); CD69 antigen; CD69 molecule; CD69_HUMAN; CLEC2C; EA1; Early activation antigen CD69; Early lymphocyte activation antigen; Early T cell activation antigen p60; Early T-cell activation antigen p60; GP32/28; Leu23; Leukocyte surface antigen Leu-23; MLR-3; MLR3; VEA; Very Early Activation Antigen;

Immunogens

Immunogen:

A synthesized peptide derived from human CD69, corresponding to a region within the internal amino acids.

Uniprot:

Q07108(CD69_HUMAN) >>Visit HPA database.

Gene(ID):
CD69(969)
Expression:
Q07108 CD69_HUMAN:

Expressed on the surface of activated T-cells, B-cells, natural killer cells, neutrophils, eosinophils, epidermal Langerhans cells and platelets.

Description:
This gene encodes a member of the calcium dependent lectin superfamily of type II transmembrane receptors. Expression of the encoded protein is induced upon activation of T lymphocytes, and may play a role in proliferation. Furthermore, the protein may act to transmit signals in natural killer cells and platelets. [provided by RefSeq, Aug 2011]
Sequence:
MSSENCFVAENSSLHPESGQENDATSPHFSTRHEGSFQVPVLCAVMNVVFITILIIALIALSVGQYNCPGQYTFSMPSDSHVSSCSEDWVGYQRKCYFISTVKRSWTSAQNACSEHGATLAVIDSEKDMNFLKRYAGREEHWVGLKKEPGHPWKWSNGKEFNNWFNVTGSDKCVFLKNTEVSSMECEKNLYWICNKPYK

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Dog
88
Sheep
86
Bovine
75
Rabbit
71
Pig
63
Horse
0
Xenopus
0
Zebrafish
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

Research Backgrounds

Function:

Involved in lymphocyte proliferation and functions as a signal transmitting receptor in lymphocytes, natural killer (NK) cells, and platelets.

PTMs:

Constitutive Ser/Thr phosphorylation in both mature thymocytes and activated T-lymphocytes.

Subcellular Location:

Membrane>Single-pass type II membrane protein.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Expressed on the surface of activated T-cells, B-cells, natural killer cells, neutrophils, eosinophils, epidermal Langerhans cells and platelets.

References

1). Excessive dissection of non-metastatic tumor-draining lymph nodes impairs immunotherapy efficacy in recurrent biliary tract cancer. Clinical cancer research : an official journal of the American Association for Cancer Research, 2025 (PubMed: 41460246) [IF=10.0]

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