Product: IKK alpha Antibody
Catalog: DF6792
Description: Rabbit polyclonal antibody to IKK alpha
Application: WB IHC IF/ICC
Reactivity: Human
Prediction: Pig, Zebrafish, Bovine, Sheep, Rabbit, Dog, Chicken, Xenopus
Mol.Wt.: 85kDa; 85kD(Calculated).
Uniprot: O15111
RRID: AB_2838754

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Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human
Prediction:
Pig(100%), Zebrafish(86%), Bovine(100%), Sheep(86%), Rabbit(100%), Dog(100%), Chicken(100%), Xenopus(100%)
Clonality:
Polyclonal
Specificity:
IKK alpha Antibody detects endogenous levels of total IKK alpha.
RRID:
AB_2838754
Cite Format: Affinity Biosciences Cat# DF6792, RRID:AB_2838754.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

chuk; CHUK1; Conserved Helix Loop Helix Ubiquitous Kinase; Conserved helix loop ubiquitous kinase; Conserved helix-loop-helix ubiquitous kinase; I Kappa B Kinase 1; I Kappa B Kinase Alpha; I-kappa-B kinase 1; I-kappa-B kinase alpha; IkappaB kinase; IkB kinase alpha subunit; IkBKA; IKK 1; IKK A; IKK a kinase; IKK-A; IKK-alpha; IKK1; IKKA; IKKA_HUMAN; Inhibitor Of Kappa Light Polypeptide Gene Enhancer In B Cells; Inhibitor Of Nuclear Factor Kappa B Kinase Alpha Subunit; Inhibitor of nuclear factor kappa-B kinase subunit alpha; NFKBIKA; Nuclear Factor Kappa B Inhibitor Kinase Alpha; Nuclear factor NF kappa B inhibitor kinase alpha; Nuclear factor NF-kappa-B inhibitor kinase alpha; Nuclear factor NFkappaB inhibitor kinase alpha; Nuclear Factor Of Kappa Light Chain Gene Enhancer In B Cells Inhibitor; TCF-16; TCF16; Transcription factor 16;

Immunogens

Immunogen:
Uniprot:
Gene(ID):
Expression:
O15111 IKKA_HUMAN:

Widely expressed.

Description:
The NF-κB/Rel transcription factors are present in the cytosol in an inactive state, complexed with the inhibitory IκB proteins (1-3). Most agents that activate NF-κB do so through a common pathway based on phosphorylation-induced, proteasome-mediated degradation of IκB (3-7). The key regulatory step in this pathway involves activation of a high molecular weight IκB kinase (IKK) complex whose catalysis is generally carried out by three tightly associated IKK subunits. IKKα and IKKβ serve as the catalytic subunits of the kinase and IKKγ serves as the regulatory subunit (8,9). Activation of IKK depends upon phosphorylation at Ser177 and Ser181 in the activation loop of IKKβ (Ser176 and Ser180 in IKKα), which causes conformational changes, resulting in kinase activation (10-13).
Sequence:
MERPPGLRPGAGGPWEMRERLGTGGFGNVCLYQHRELDLKIAIKSCRLELSTKNRERWCHEIQIMKKLNHANVVKACDVPEELNILIHDVPLLAMEYCSGGDLRKLLNKPENCCGLKESQILSLLSDIGSGIRYLHENKIIHRDLKPENIVLQDVGGKIIHKIIDLGYAKDVDQGSLCTSFVGTLQYLAPELFENKPYTATVDYWSFGTMVFECIAGYRPFLHHLQPFTWHEKIKKKDPKCIFACEEMSGEVRFSSHLPQPNSLCSLVVEPMENWLQLMLNWDPQQRGGPVDLTLKQPRCFVLMDHILNLKIVHILNMTSAKIISFLLPPDESLHSLQSRIERETGINTGSQELLSETGISLDPRKPASQCVLDGVRGCDSYMVYLFDKSKTVYEGPFASRSLSDCVNYIVQDSKIQLPIIQLRKVWAEAVHYVSGLKEDYSRLFQGQRAAMLSLLRYNANLTKMKNTLISASQQLKAKLEFFHKSIQLDLERYSEQMTYGISSEKMLKAWKEMEEKAIHYAEVGVIGYLEDQIMSLHAEIMELQKSPYGRRQGDLMESLEQRAIDLYKQLKHRPSDHSYSDSTEMVKIIVHTVQSQDRVLKELFGHLSKLLGCKQKIIDLLPKVEVALSNIKEADNTVMFMQGKRQKEIWHLLKIACTQSSARSLVGSSLEGAVTPQTSAWLPPTSAEHDHSLSCVVTPQDGETSAQMIEENLNCLGHLSTIIHEANEEQGNSMMNLDWSWLTE

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Bovine
100
Dog
100
Xenopus
100
Chicken
100
Rabbit
100
Sheep
86
Zebrafish
86
Horse
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - O15111 As Substrate

Site PTM Type Enzyme
T23 Phosphorylation Q9Y243 (AKT3) , O00141 (SGK1) , P31749 (AKT1) , P31751 (AKT2)
K44 Ubiquitination
K53 Ubiquitination
K67 Ubiquitination
K105 Ubiquitination
K109 Ubiquitination
K117 Ubiquitination
S119 Acetylation
K139 Ubiquitination
K146 Ubiquitination
K158 Ubiquitination
Y168 Phosphorylation
S176 Phosphorylation Q04759 (PRKCQ) , Q99558 (MAP3K14)
T179 Acetylation
S180 Phosphorylation Q04759 (PRKCQ) , Q99558 (MAP3K14)
K296 Ubiquitination
K322 Acetylation
K366 Ubiquitination
K391 Ubiquitination
K415 Ubiquitination
K438 Acetylation
T463 Phosphorylation
S473 Phosphorylation Q13315 (ATM)
Y500 Phosphorylation
K506 Ubiquitination
S536 Phosphorylation
K569 Ubiquitination
K572 Ubiquitination
S576 Phosphorylation
T593 Phosphorylation
K602 Ubiquitination
K615 Ubiquitination
K617 Ubiquitination
K624 Ubiquitination
T722 Acetylation

PTMs - O15111 As Enzyme

Substrate Site Source
O14920-1 (IKBKB) S177 Uniprot
O14920-1 (IKBKB) S181 Uniprot
O43524 (FOXO3) S644 Uniprot
O75925-1 (PIAS1) S90 Uniprot
P03372 (ESR1) S118 Uniprot
P19838-2 (NFKB1) S922 Uniprot
P19838 (NFKB1) S923 Uniprot
P19838-2 (NFKB1) S924 Uniprot
P19838 (NFKB1) S927 Uniprot
P19838-2 (NFKB1) S928 Uniprot
P19838 (NFKB1) T931 Uniprot
P19838 (NFKB1) S932 Uniprot
P19838-2 (NFKB1) T932 Uniprot
P19838-2 (NFKB1) S933 Uniprot
P24385 (CCND1) T286 Uniprot
P25963 (NFKBIA) S32 Uniprot
P25963 (NFKBIA) S36 Uniprot
P35222 (CTNNB1) S33 Uniprot
P35222 (CTNNB1) S37 Uniprot
P35222 (CTNNB1) T41 Uniprot
P35222 (CTNNB1) S45 Uniprot
P42345 (MTOR) S1415 Uniprot
P84243 (H3F3B) S11 Uniprot
Q00653 (NFKB2) S99 Uniprot
Q00653 (NFKB2) S108 Uniprot
Q00653 (NFKB2) S115 Uniprot
Q00653 (NFKB2) S123 Uniprot
Q00653 (NFKB2) S872 Uniprot
Q01094 (E2F1) S403 Uniprot
Q01201 (RELB) S472 Uniprot
Q04206 (RELA) S536 Uniprot
Q04864 (REL) S557 Uniprot
Q14164 (IKBKE) S172 Uniprot
Q15653-1 (NFKBIB) S19 Uniprot
Q15653-1 (NFKBIB) S23 Uniprot
Q15717 (ELAVL1) S304 Uniprot
Q5S007 (LRRK2) S910 Uniprot
Q5S007 (LRRK2) S935 Uniprot
Q86VP1-2 (TAX1BP1) S593 Uniprot
Q86VP1-2 (TAX1BP1) S624 Uniprot
Q86VP1 (TAX1BP1) S666 Uniprot
Q92793 (CREBBP) S1382 Uniprot
Q92793 (CREBBP) S1386 Uniprot
Q99558 (MAP3K14) T559 Uniprot
Q9BUZ4 (TRAF4) S426 Uniprot
Q9BXH1 (BBC3) S10 Uniprot
Q9NQC7 (CYLD) S418 Uniprot
Q9NQC7 (CYLD) S422 Uniprot
Q9NQC7 (CYLD) S432 Uniprot
Q9NQC7 (CYLD) S436 Uniprot
Q9NQC7 (CYLD) S439 Uniprot
Q9NQC7 (CYLD) S441 Uniprot
Q9NQC7 (CYLD) S444 Uniprot
Q9Y261 (FOXA2) S107 Uniprot
Q9Y261 (FOXA2) S111 Uniprot
Q9Y6Q9-5 (NCOA3) S857 Uniprot

Research Backgrounds

Function:

Serine kinase that plays an essential role in the NF-kappa-B signaling pathway which is activated by multiple stimuli such as inflammatory cytokines, bacterial or viral products, DNA damages or other cellular stresses. Acts as part of the canonical IKK complex in the conventional pathway of NF-kappa-B activation and phosphorylates inhibitors of NF-kappa-B on serine residues. These modifications allow polyubiquitination of the inhibitors and subsequent degradation by the proteasome. In turn, free NF-kappa-B is translocated into the nucleus and activates the transcription of hundreds of genes involved in immune response, growth control, or protection against apoptosis. Negatively regulates the pathway by phosphorylating the scaffold protein TAXBP1 and thus promoting the assembly of the A20/TNFAIP3 ubiquitin-editing complex (composed of A20/TNFAIP3, TAX1BP1, and the E3 ligases ITCH and RNF11). Therefore, CHUK plays a key role in the negative feedback of NF-kappa-B canonical signaling to limit inflammatory gene activation. As part of the non-canonical pathway of NF-kappa-B activation, the MAP3K14-activated CHUK/IKKA homodimer phosphorylates NFKB2/p100 associated with RelB, inducing its proteolytic processing to NFKB2/p52 and the formation of NF-kappa-B RelB-p52 complexes. In turn, these complexes regulate genes encoding molecules involved in B-cell survival and lymphoid organogenesis. Participates also in the negative feedback of the non-canonical NF-kappa-B signaling pathway by phosphorylating and destabilizing MAP3K14/NIK. Within the nucleus, phosphorylates CREBBP and consequently increases both its transcriptional and histone acetyltransferase activities. Modulates chromatin accessibility at NF-kappa-B-responsive promoters by phosphorylating histones H3 at 'Ser-10' that are subsequently acetylated at 'Lys-14' by CREBBP. Additionally, phosphorylates the CREBBP-interacting protein NCOA3. Also phosphorylates FOXO3 and may regulate this pro-apoptotic transcription factor. Phosphorylates RIPK1 at 'Ser-25' which represses its kinase activity and consequently prevents TNF-mediated RIPK1-dependent cell death (By similarity).

PTMs:

Phosphorylated by MAP3K14/NIK, AKT and to a lesser extent by MEKK1, and dephosphorylated by PP2A. Autophosphorylated.

(Microbial infection) Acetylation of Thr-179 by Yersinia yopJ prevents phosphorylation and activation, thus blocking the I-kappa-B signaling pathway.

Subcellular Location:

Cytoplasm. Nucleus.
Note: Shuttles between the cytoplasm and the nucleus.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Widely expressed.

Subunit Structure:

Component of the I-kappa-B-kinase (IKK) core complex consisting of CHUK, IKBKB and IKBKG; probably four alpha/CHUK-beta/IKBKB dimers are associated with four gamma/IKBKG subunits. The IKK core complex seems to associate with regulatory or adapter proteins to form a IKK-signalosome holo-complex. The IKK complex associates with TERF2IP/RAP1, leading to promote IKK-mediated phosphorylation of RELA/p65 (By similarity). Part of a complex composed of NCOA2, NCOA3, CHUK/IKKA, IKBKB, IKBKG and CREBBP. Part of a 70-90 kDa complex at least consisting of CHUK/IKKA, IKBKB, NFKBIA, RELA, ELP1 and MAP3K14. Directly interacts with TRPC4AP (By similarity). May interact with TRAF2. Interacts with NALP2. May interact with MAVS/IPS1. Interacts with ARRB1 and ARRB2. Interacts with NLRC5; prevents CHUK phosphorylation and kinase activity. Interacts with PIAS1; this interaction induces PIAS1 phosphorylation. Interacts with ZNF268 isoform 2; the interaction is further increased in a TNF-alpha-dependent manner. Interacts with FOXO3. Interacts with IFIT5; the interaction synergizes the recruitment of IKK to MAP3K7 and enhances IKK phosphorylation. Interacts with LRRC14. Interacts with SASH1.

(Microbial infection) Interacts with InlC of Listeria monocytogenes.

Family&Domains:

The kinase domain is located in the N-terminal region. The leucine zipper is important to allow homo- and hetero-dimerization. At the C-terminal region is located the region responsible for the interaction with NEMO/IKBKG.

Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. I-kappa-B kinase subfamily.

Research Fields

· Cellular Processes > Cell growth and death > Apoptosis.   (View pathway)

· Environmental Information Processing > Signal transduction > MAPK signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > Ras signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > NF-kappa B signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > FoxO signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > mTOR signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > PI3K-Akt signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > TNF signaling pathway.   (View pathway)

· Human Diseases > Drug resistance: Antineoplastic > Antifolate resistance.

· Human Diseases > Infectious diseases: Bacterial > Epithelial cell signaling in Helicobacter pylori infection.

· Human Diseases > Infectious diseases: Bacterial > Shigellosis.

· Human Diseases > Infectious diseases: Parasitic > Chagas disease (American trypanosomiasis).

· Human Diseases > Infectious diseases: Parasitic > Toxoplasmosis.

· Human Diseases > Infectious diseases: Viral > Hepatitis C.

· Human Diseases > Infectious diseases: Viral > Hepatitis B.

· Human Diseases > Infectious diseases: Viral > Measles.

· Human Diseases > Infectious diseases: Viral > Human papillomavirus infection.

· Human Diseases > Infectious diseases: Viral > HTLV-I infection.

· Human Diseases > Infectious diseases: Viral > Herpes simplex infection.

· Human Diseases > Infectious diseases: Viral > Epstein-Barr virus infection.

· Human Diseases > Cancers: Overview > Pathways in cancer.   (View pathway)

· Human Diseases > Cancers: Specific types > Pancreatic cancer.   (View pathway)

· Human Diseases > Cancers: Specific types > Prostate cancer.   (View pathway)

· Human Diseases > Cancers: Specific types > Chronic myeloid leukemia.   (View pathway)

· Human Diseases > Cancers: Specific types > Acute myeloid leukemia.   (View pathway)

· Human Diseases > Cancers: Specific types > Small cell lung cancer.   (View pathway)

· Organismal Systems > Immune system > Chemokine signaling pathway.   (View pathway)

· Organismal Systems > Development > Osteoclast differentiation.   (View pathway)

· Organismal Systems > Immune system > Toll-like receptor signaling pathway.   (View pathway)

· Organismal Systems > Immune system > NOD-like receptor signaling pathway.   (View pathway)

· Organismal Systems > Immune system > RIG-I-like receptor signaling pathway.   (View pathway)

· Organismal Systems > Immune system > Cytosolic DNA-sensing pathway.   (View pathway)

· Organismal Systems > Immune system > IL-17 signaling pathway.   (View pathway)

· Organismal Systems > Immune system > Th1 and Th2 cell differentiation.   (View pathway)

· Organismal Systems > Immune system > Th17 cell differentiation.   (View pathway)

· Organismal Systems > Immune system > T cell receptor signaling pathway.   (View pathway)

· Organismal Systems > Immune system > B cell receptor signaling pathway.   (View pathway)

· Organismal Systems > Endocrine system > Adipocytokine signaling pathway.

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