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$280 100ul
$350 200ul

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  • Product Name
    Zyxin Antibody
  • Catalog No.
    DF6858
  • RRID
    AB_2838817
  • Source
    Rabbit
  • Application
    WB,IHC,IF/ICC,ELISA
  • Reactivity
    Human, Mouse, Rat
  • Prediction
    Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%)
  • UniProt
  • Mol.Wt
    80kD,70kD;
    61kDa(Calculated).
  • Concentration
    1mg/ml
  • Browse similar products>>

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Product Information

Alternative Names:Expand▼

ESP 2; ESP2; HED 2; HED2; ZYX; ZYX protein; Zyxin 2; Zyxin2;

Applications:

WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500, ELISA(peptide) 1:20000-1:40000
*The optimal dilutions should be determined by the end user.

Reactivity:

Human, Mouse, Rat

Predicted Reactivity:

Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%)

Source:

Rabbit

Clonality:

Polyclonal

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Specificity:

Zyxin Antibody detects endogenous levels of total Zyxin.

RRID:

AB_2838817
Please cite this product as: Affinity Biosciences Cat# DF6858, RRID:AB_2838817.

Format:

Liquid

Concentration:

1mg/ml

Storage Condition and Buffer:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt.

Immunogen Information

Immunogen:

A synthesized peptide derived from human Zyxin, corresponding to a region within the internal amino acids.

Uniprot:



>>Visit The Human Protein Atlas

Gene ID:

Gene Name:

ZYX

Molecular Weight:

Observed Mol.Wt.: 80kD,70kD.
Predicted Mol.Wt.: 61kDa(Calculated)..

Subcellular Location:

Cytoplasmic, cytoskeletal and Nuclear.

Description:

The zyxin family of proteins includes LIMD1, ajuba, trip6 and zyxin, each of which contains three LIM domains at the carboxy-terminus. Zyxin family members associate with the actin cytoskeleton and are components of both the cell-cell junction adhesive complex and the integrin-mediated adhesive complex. They shuttle in and out of the nucleus where they may function in transcriptional activation (1).Zyxin is involved in the regulation of mechanical force-induced actin polymerization at focal adhesions (2), and in regulation of adhesion and migration, possibly through recruitment of Ena/VASP proteins to focal adhesions (3). Zyxin interacts with and may regulate the function of the tumor suppressor myopodin, which inhibits tumor growth and metastasis (4).

Sequence:
MAAPRPSPAISVSVSAPAFYAPQKKFGPVVAPKPKVNPFRPGDSEPPPAPGAQRAQMGRVGEIPPPPPEDFPLPPPPLAGDGDDAEGALGGAFPPPPPPIEESFPPAPLEEEIFPSPPPPPEEEGGPEAPIPPPPQPREKVSSIDLEIDSLSSLLDDMTKNDPFKARVSSGYVPPPVATPFSSKSSTKPAAGGTAPLPPWKSPSSSQPLPQVPAPAQSQTQFHVQPQPQPKPQVQLHVQSQTQPVSLANTQPRGPPASSPAPAPKFSPVTPKFTPVASKFSPGAPGGSGSQPNQKLGHPEALSAGTGSPQPPSFTYAQQREKPRVQEKQHPVPPPAQNQNQVRSPGAPGPLTLKEVEELEQLTQQLMQDMEHPQRQNVAVNELCGRCHQPLARAQPAVRALGQLFHIACFTCHQCAQQLQGQQFYSLEGAPYCEGCYTDTLEKCNTCGEPITDRMLRATGKAYHPHCFTCVVCARPLEGTSFIVDQANRPHCVPDYHKQYAPRCSVCSEPIMPEPGRDETVRVVALDKNFHMKCYKCEDCGKPLSIEADDNGCFPLDGHVLCRKCHTARAQT

Research Background

Function:

Adhesion plaque protein. Binds alpha-actinin and the CRP protein. Important for targeting TES and ENA/VASP family members to focal adhesions and for the formation of actin-rich structures. May be a component of a signal transduction pathway that mediates adhesion-stimulated changes in gene expression (By similarity).

Subcellular Location:

Cytoplasm. Cytoplasm>Cytoskeleton. Nucleus. Cell junction>Focal adhesion.
Note: Associates with the actin cytoskeleton near the adhesion plaques. Enters the nucleus in the presence of HESX1.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte

Subunit Structure:

Interacts with HPV type 6 protein E6. Does not interact significantly with E6 proteins from HPV types 11, 16, or 18. Interacts, via the Pro-rich regions, with the EVH1 domains of ENAH, EVL and VASP. Interacts with the first LIM domain of TES. Interacts with NEBL (isoform 2). Interacts with SYNPO2.

(Microbial infection) Interacts with human papillomavirus type 6/HPV6 protein E6. Does not interact significantly with E6 proteins from HPV types 11, 16, or 18.

Similarity:

Belongs to the zyxin/ajuba family.

Research Fields

Research Fields:

· Cellular Processes > Cellular community - eukaryotes > Focal adhesion.(View pathway)

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Catalog Number :

DF6858-BP
(Blocking peptide available as DF6858-BP)

Price/Size :

$350/1mg.
Tips: For phospho antibody, we provide phospho peptide(0.5mg) and non-phospho peptide(0.5mg).

Function :

Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. This mechanism is useful when non-specific binding is an issue, for example, in Western blotting (immunoblot) and immunohistochemistry (IHC). By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific; Specific binding will be absent from the western blot or immunostaining performed with the neutralized antibody.

Format and storage :

Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 10 mg/ml.The purity is >90%,tested by HPLC and MS.Storage Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C.

Precautions :

This product is for research use only. Not for use in diagnostic or therapeutic procedures.

Pig
100%
Rabbit
100%
Sheep
100%
Dog
100%
Bovine
100%
Horse
100%
Chicken
0%
Zebrafish
0%
Xenopus
0%
High similarity Medium similarity Low similarity No similarity
Q15942 as Substrate
Site PTM Type Enzyme
A2 Acetylation
R5 Methylation
S7 Phosphorylation
S13 Phosphorylation
S15 Phosphorylation
Y20 Phosphorylation
K24 Acetylation
K25 Acetylation
K25 Methylation
S44 Phosphorylation
S116 Phosphorylation
S142 Phosphorylation P31749 (AKT1)
S143 Phosphorylation
S150 Phosphorylation
K165 Acetylation
K165 Methylation
K165 Ubiquitination
S169 Phosphorylation
S170 Phosphorylation
Y172 Phosphorylation
T179 Phosphorylation
K201 Methylation
S202 Phosphorylation
S206 Phosphorylation
T250 Phosphorylation
S258 Phosphorylation
S259 Phosphorylation
K265 Acetylation
K265 Ubiquitination
S267 Phosphorylation
T270 Phosphorylation
K272 Acetylation
K272 Methylation
K272 Ubiquitination
T274 Phosphorylation
S278 Phosphorylation
K279 Acetylation
K279 Methylation
K279 Ubiquitination
S281 Phosphorylation
S288 Phosphorylation
S290 Phosphorylation
K295 Ubiquitination
S303 Phosphorylation P24941 (CDK2)
T306 Phosphorylation P24941 (CDK2)
S308 Phosphorylation P24941 (CDK2)
S313 Phosphorylation
T315 Phosphorylation
Y316 Phosphorylation
K328 Ubiquitination
R343 Methylation
S344 Phosphorylation
T352 Phosphorylation
S505 Phosphorylation
K528 Acetylation
IMPORTANT: For western blots, incubate membrane with diluted antibody in 5% w/v milk , 1X TBS, 0.1% Tween®20 at 4°C with gentle shaking, overnight.

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