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  • Product Name
    ZBTB16 Antibody
  • Catalog No.
    BF0221
  • RRID
    AB_2833826
  • Source
    Mouse
  • Application
    WB,IF/ICC,ELISA
  • Reactivity
    Human
  • UniProt
  • Mol.Wt
    74kD;
    74kDa(Calculated).
  • Concentration
    1mg/ml
  • Browse similar products>>

Product Information

Alternative Names:Expand▼

AI467657; lu; PLZF; Promyelocytic leukemia zinc finger protein; ZBT16_HUMAN; ZBTB16; Zfp145; Zinc finger and BTB domain containing 16; zinc finger and BTB domain containing protein 16; Zinc finger and BTB domain-containing protein 16; Zinc finger protein 145 (Kruppel like expressed in promyelocytic leukemia); Zinc finger protein 145; Zinc finger protein PLZF; Znf145;

Applications:

ELISA 1:10000, WB 1:500-1:2000, IF/ICC 1:200-1:1000
*The optimal dilutions should be determined by the end user.

Reactivity:

Human

Source:

Mouse

Clonality:

Monoclonal

Clone Number:

AFB1802

Purification:

Affinity-chromatography.

Specificity:

ZBTB16 antibody detects endogenous levels of total ZBTB16.

RRID:

AB_2833826
Please cite this product as: Affinity Biosciences Cat# BF0221, RRID:AB_2833826.

Format:

Liquid

Concentration:

1mg/ml

Storage Condition and Buffer:

Mouse IgG1 in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt.

Immunogen Information

Immunogen:

Purified recombinant fragment of human ZBTB16 expressed in E. Coli.

Uniprot:



>>Visit The Human Protein Atlas

Gene ID:

Gene Name:

ZBTB16

Molecular Weight:

Observed Mol.Wt.: 74kD.
Predicted Mol.Wt.: 74kDa(Calculated)..

Subcellular Location:

Nucleus.

Tissue Specificity:

Q05516 ZBT16_HUMAN:
Within the hematopoietic system, PLZF is expressed in bone marrow, early myeloid cell lines and peripheral blood mononuclear cells. Also expressed in the ovary, and at lower levels, in the kidney and lung.

Description:

This gene is a member of the Krueppel C2H2-type zinc-finger protein family and encodes a zinc finger transcription factor that contains nine Kruppel-type zinc finger domains at the carboxyl terminus. This protein is located in the nucleus, is involved in cell cycle progression, and interacts with a histone deacetylase. Specific instances of aberrant gene rearrangement at this locus have been associated with acute promyelocytic leukemia (APL). Alternate transcriptional splice variants have been characterized. Tissue specificity: Within the hematopoietic system, PLZF is expressed in bone marrow, early myeloid cell lines and peripheral blood mononuclear cells. Also expressed in the ovary, and at lower levels, in the kidney and lung.

Sequence:
MDLTKMGMIQLQNPSHPTGLLCKANQMRLAGTLCDVVIMVDSQEFHAHRTVLACTSKMFEILFHRNSQHYTLDFLSPKTFQQILEYAYTATLQAKAEDLDDLLYAAEILEIEYLEEQCLKMLETIQASDDNDTEATMADGGAEEEEDRKARYLKNIFISKHSSEESGYASVAGQSLPGPMVDQSPSVSTSFGLSAMSPTKAAVDSLMTIGQSLLQGTLQPPAGPEEPTLAGGGRHPGVAEVKTEMMQVDEVPSQDSPGAAESSISGGMGDKVEERGKEGPGTPTRSSVITSARELHYGREESAEQVPPPAEAGQAPTGRPEHPAPPPEKHLGIYSVLPNHKADAVLSMPSSVTSGLHVQPALAVSMDFSTYGGLLPQGFIQRELFSKLGELAVGMKSESRTIGEQCSVCGVELPDNEAVEQHRKLHSGMKTYGCELCGKRFLDSLRLRMHLLAHSAGAKAFVCDQCGAQFSKEDALETHRQTHTGTDMAVFCLLCGKRFQAQSALQQHMEVHAGVRSYICSECNRTFPSHTALKRHLRSHTGDHPYECEFCGSCFRDESTLKSHKRIHTGEKPYECNGCGKKFSLKHQLETHYRVHTGEKPFECKLCHQRSRDYSAMIKHLRTHNGASPYQCTICTEYCPSLSSMQKHMKGHKPEEIPPDWRIEKTYLYLCYV

Research Background

Function:

Acts as a transcriptional repressor. May play a role in myeloid maturation and in the development and/or maintenance of other differentiated tissues. Probable substrate-recognition component of an E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins.

Subcellular Location:

Nucleus. Nucleus>Nuclear body.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte

Tissue Specificity:

Within the hematopoietic system, PLZF is expressed in bone marrow, early myeloid cell lines and peripheral blood mononuclear cells. Also expressed in the ovary, and at lower levels, in the kidney and lung.

Subunit Structure:

Binds EPN1. Interacts with ZBTB32 and CUL3. Interacts with ATP7B. Interacts (via C2H2-type zinc finger domains 1 and 2) with RNF112.

Similarity:

Belongs to the krueppel C2H2-type zinc-finger protein family.

Research Fields

Research Fields:

· Human Diseases > Cancers: Overview > Pathways in cancer.(View pathway)
· Human Diseases > Cancers: Specific types > Acute myeloid leukemia.(View pathway)
· Human Diseases > Cancers: Overview > Transcriptional misregulation in cancer.

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Catalog Number :

BF0221-BP
(Blocking peptide available as BF0221-BP)

Price/Size :

$350/1mg.
Tips: For phospho antibody, we provide phospho peptide(0.5mg) and non-phospho peptide(0.5mg).

Function :

Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. This mechanism is useful when non-specific binding is an issue, for example, in Western blotting (immunoblot) and immunohistochemistry (IHC). By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific; Specific binding will be absent from the western blot or immunostaining performed with the neutralized antibody.

Format and storage :

Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 10 mg/ml.The purity is >90%,tested by HPLC and MS.Storage Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C.

Precautions :

This product is for research use only. Not for use in diagnostic or therapeutic procedures.

Q05516 as Substrate
Site PTM Type Enzyme
K154 Ubiquitination
S184 Phosphorylation
S197 Phosphorylation P24941 (CDK2)
K242 Sumoylation
T282 Phosphorylation P24941 (CDK2)
Y334 Phosphorylation
K387 Methylation
K387 Sumoylation
K396 Methylation
K396 Sumoylation
T569 Phosphorylation
T597 Phosphorylation
Y630 Phosphorylation
K647 Acetylation
K650 Acetylation
K653 Acetylation
IMPORTANT: For western blots, incubate membrane with diluted antibody in 5% w/v milk , 1X TBS, 0.1% Tween®20 at 4°C with gentle shaking, overnight.

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