Product: Tri-Methyl-Histone H3 (Lys27)/H3K27me3 Antibody
Catalog: DF6941
Description: Rabbit polyclonal antibody to Tri-Methyl-Histone H3 (Lys27)/H3K27me3
Application: WB IHC IF/ICC IP CHIP
Reactivity: Human, Mouse, Rat
Mol.Wt.: 15kDa; 16kD(Calculated).
Uniprot: Q16695
RRID: AB_2838900

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 100ul $280 In stock
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Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:50-1:200, IP 1:50-1:200, CHIP 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Clonality:
Polyclonal
Specificity:
Tri-Methyl-Histone H3 (Lys27)/H3K27me3 Antibody detects endogenous levels of Tri-Methyl-Histone H3 only when methylated at Lys27.
RRID:
AB_2838900
Cite Format: Affinity Biosciences Cat# DF6941, RRID:AB_2838900.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

;H3.3A; HIST1 cluster, H3E; H3 histone family, member A; H3.1; H3/l; H3F3; H3FF; H3FJ; H3FL; Histone gene cluster 1, H3 histone family, member E; histone H3.1t; Histone H3/o; FLJ92264; H 3; H3; H3 histone family, member B; H3 histone family, member C; H3 histone family, member D; H3 histone family, member F; H3 histone family, member H; H3 histone family, member I; H3 histone family, member J; H3 histone family, member K; H3 histone family, member L; H3 histone family, member T; H3 histone, family 3A; H3/A; H3/b; H3/c; H3/d; h3/f; H3/h; H3/i; H3/j; H3/k; H3/t; H31_HUMAN; H3F1K; H3F3A; H3FA; H3FB; H3FC; H3FD; H3FH; H3FI; H3FK; HIST1 cluster, H3A; HIST1 cluster, H3B; HIST1 cluster, H3C; HIST1 cluster, H3D; HIST1 cluster, H3F; HIST1 cluster, H3G; HIST1 cluster, H3H; HIST1 cluster, H3I; HIST1 cluster, H3J; HIST1H3A; HIST1H3B; HIST1H3C; HIST1H3D; HIST1H3E; HIST1H3F; HIST1H3G; HIST1H3H; HIST1H3I; HIST1H3J; HIST3H3; Histone 1, H3a; Histone 1, H3b; Histone 1, H3c; Histone 1, H3d; Histone 1, H3e; Histone 1, H3f; Histone 1, H3g; Histone 1, H3h; Histone 1, H3i; Histone 3, H3; histone cluster 1 H3 family member a; histone cluster 1 H3 family member b; histone cluster 1 H3 family member c; histone cluster 1 H3 family member d; histone cluster 1 H3 family member e; histone cluster 1 H3 family member f; histone cluster 1 H3 family member g; histone cluster 1 H3 family member h; histone cluster 1 H3 family member i; histone cluster 1 H3 family member j; Histone cluster 1, H3a; Histone cluster 1, H3b; Histone cluster 1, H3c; Histone cluster 1, H3d; Histone cluster 1, H3e; Histone cluster 1, H3f; Histone cluster 1, H3g; Histone cluster 1, H3i; Histone cluster 1, H3j; Histone gene cluster 1, H3 histone family, member A; Histone gene cluster 1, H3 histone family, member B; Histone gene cluster 1, H3 histone family, member C; Histone gene cluster 1, H3 histone family, member D; Histone gene cluster 1, H3 histone family, member F; Histone gene cluster 1, H3 histone family, member G; Histone gene cluster 1, H3 histone family, member H; Histone gene cluster 1, H3 histone family, member I; Histone gene cluster 1, H3 histone family, member J; Histone gene cluster 1, H3A; Histone gene cluster 1, H3B; Histone gene cluster 1, H3C; Histone gene cluster 1, H3D; Histone gene cluster 1, H3E; Histone gene cluster 1, H3F; Histone gene cluster 1, H3G; Histone gene cluster 1, H3H; Histone gene cluster 1, H3I; Histone gene cluster 1, H3J; Histone H 3; Histone H3.1; Histone H3.2; Histone H3.3; Histone H3/a; Histone H3/b; Histone H3/c; Histone H3/d; Histone H3/f; Histone H3/h; Histone H3/i; Histone H3/j; Histone H3/k; Histone H3/l; Histone H3/m;

Immunogens

Immunogen:
Uniprot:
Gene(ID):
Expression:
Q16695 H31T_HUMAN:

Expressed in testicular cells.

Description:
Modulation of chromatin structure plays an important role in the regulation of transcription in eukaryotes. The nucleosome, made up of DNA wound around eight core histone proteins (two each of H2A, H2B, H3, and H4), is the primary building block of chromatin (1). The amino-terminal tails of core histones undergo various post-translational modifications, including acetylation, phosphorylation, methylation, and ubiquitination (2-5). These modifications occur in response to various stimuli and have a direct effect on the accessibility of chromatin to transcription factors and, therefore, gene expression (6). In most species, histone H2B is primarily acetylated at Lys5, 12, 15, and 20 (4,7). Histone H3 is primarily acetylated at Lys9, 14, 18, 23, 27, and 56. Acetylation of H3 at Lys9 appears to have a dominant role in histone deposition and chromatin assembly in some organisms (2,3). Phosphorylation at Ser10, Ser28, and Thr11 of histone H3 is tightly correlated with chromosome condensation during both mitosis and meiosis (8-10). Phosphorylation at Thr3 of histone H3 is highly conserved among many species and is catalyzed by the kinase haspin. Immunostaining with phospho-specific antibodies in mammalian cells reveals mitotic phosphorylation at Thr3 of H3 in prophase and its dephosphorylation during anaphase (11).
Sequence:
MARTKQTARKSTGGKAPRKQLATKVARKSAPATGGVKKPHRYRPGTVALREIRRYQKSTELLIRKLPFQRLMREIAQDFKTDLRFQSSAVMALQEACESYLVGLFEDTNLCVIHAKRVTIMPKDIQLARRIRGERA

PTMs - Q16695 As Substrate

Site PTM Type Enzyme
K5 Methylation
K10 Acetylation
K10 Methylation
S11 Phosphorylation Q13557 (CAMK2D) , O75582 (RPS6KA5) , P51812 (RPS6KA3)
T12 Phosphorylation
K15 Acetylation
K15 Methylation
K15 Sumoylation
K15 Ubiquitination
K19 Acetylation
K24 Acetylation
K24 Methylation
K28 Acetylation
K28 Methylation
K28 Ubiquitination
S29 Phosphorylation O75582 (RPS6KA5)
T33 Phosphorylation
K37 Acetylation
K37 Methylation
K37 Ubiquitination
K38 Methylation
K38 Ubiquitination
R41 Methylation
Y42 Phosphorylation
R43 Methylation
T46 Phosphorylation
R50 Methylation
K57 Acetylation
K57 Sumoylation
K57 Ubiquitination
S58 Phosphorylation
T59 Phosphorylation
R64 Methylation
K65 Methylation
K80 Acetylation
K80 Methylation
K80 Sumoylation
K80 Ubiquitination
T81 Phosphorylation
R84 Methylation
S87 Phosphorylation
S88 Phosphorylation
S99 Phosphorylation
T108 Phosphorylation
T119 Phosphorylation
K123 Acetylation
K123 Sumoylation
K123 Ubiquitination
R129 Methylation

Research Backgrounds

Function:

Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.

PTMs:

Acetylation is generally linked to gene activation. Acetylation on Lys-10 (H3K9ac) impairs methylation at Arg-9 (H3R8me2s). Acetylation on Lys-19 (H3K18ac) and Lys-24 (H3K24ac) favors methylation at Arg-18 (H3R17me). Acetylation at Lys-123 (H3K122ac) by EP300/p300 plays a central role in chromatin structure: localizes at the surface of the histone octamer and stimulates transcription, possibly by promoting nucleosome instability (By similarity).

Citrullination at Arg-9 (H3R8ci) and/or Arg-18 (H3R17ci) by PADI4 impairs methylation and represses transcription.

Asymmetric dimethylation at Arg-18 (H3R17me2a) by CARM1 is linked to gene activation. Symmetric dimethylation at Arg-9 (H3R8me2s) by PRMT5 is linked to gene repression. Asymmetric dimethylation at Arg-3 (H3R2me2a) by PRMT6 is linked to gene repression and is mutually exclusive with H3 Lys-5 methylation (H3K4me2 and H3K4me3). H3R2me2a is present at the 3' of genes regardless of their transcription state and is enriched on inactive promoters, while it is absent on active promoters (By similarity).

Methylation at Lys-5 (H3K4me), Lys-37 (H3K36me) and Lys-80 (H3K79me) are linked to gene activation. Methylation at Lys-5 (H3K4me) facilitates subsequent acetylation of H3 and H4. Methylation at Lys-80 (H3K79me) is associated with DNA double-strand break (DSB) responses and is a specific target for TP53BP1. Methylation at Lys-10 (H3K9me) and Lys-28 (H3K27me) are linked to gene repression. Methylation at Lys-10 (H3K9me) is a specific target for HP1 proteins (CBX1, CBX3 and CBX5) and prevents subsequent phosphorylation at Ser-11 (H3S10ph) and acetylation of H3 and H4. Methylation at Lys-5 (H3K4me) and Lys-80 (H3K79me) require preliminary monoubiquitination of H2B at 'Lys-120'. Methylation at Lys-10 (H3K9me) and Lys-28 (H3K27me) are enriched in inactive X chromosome chromatin. Monomethylation at Lys-57 (H3K56me1) by EHMT2/G9A in G1 phase promotes interaction with PCNA and is required for DNA replication (By similarity).

Phosphorylated at Thr-4 (H3T3ph) by HASPIN during prophase and dephosphorylated during anaphase. Phosphorylation at Ser-11 (H3S10ph) by AURKB is crucial for chromosome condensation and cell-cycle progression during mitosis and meiosis. In addition phosphorylation at Ser-11 (H3S10ph) by RPS6KA4 and RPS6KA5 is important during interphase because it enables the transcription of genes following external stimulation, like mitogens, stress, growth factors or UV irradiation and result in the activation of genes, such as c-fos and c-jun. Phosphorylation at Ser-11 (H3S10ph), which is linked to gene activation, prevents methylation at Lys-10 (H3K9me) but facilitates acetylation of H3 and H4. Phosphorylation at Ser-11 (H3S10ph) by AURKB mediates the dissociation of HP1 proteins (CBX1, CBX3 and CBX5) from heterochromatin. Phosphorylation at Ser-11 (H3S10ph) is also an essential regulatory mechanism for neoplastic cell transformation. Phosphorylated at Ser-29 (H3S28ph) by MAP3K20 isoform 1, RPS6KA5 or AURKB during mitosis or upon ultraviolet B irradiation. Phosphorylation at Thr-7 (H3T6ph) by PRKCB is a specific tag for epigenetic transcriptional activation that prevents demethylation of Lys-5 (H3K4me) by LSD1/KDM1A. At centromeres, specifically phosphorylated at Thr-12 (H3T11ph) from prophase to early anaphase, by DAPK3 and PKN1. Phosphorylation at Thr-12 (H3T11ph) by PKN1 is a specific tag for epigenetic transcriptional activation that promotes demethylation of Lys-10 (H3K9me) by KDM4C/JMJD2C. Phosphorylation at Tyr-42 (H3Y41ph) by JAK2 promotes exclusion of CBX5 (HP1 alpha) from chromatin (By similarity).

Ubiquitinated.

Lysine deamination at Lys-5 (H3K4all) to form allysine is mediated by LOXL2. Allysine formation by LOXL2 only takes place on H3K4me3 and results in gene repression (By similarity).

Butyrylation of histones marks active promoters and competes with histone acetylation. It is present during late spermatogenesis.

Succinylation at Lys-80 (H3K79succ) by KAT2A takes place with a maximum frequency around the transcription start sites of genes. It gives a specific tag for epigenetic transcription activation. Desuccinylation at Lys-123 (H3K122succ) by SIRT7 in response to DNA damage promotes chromatin condensation and double-strand breaks (DSBs) repair.

Serine ADP-ribosylation constitutes the primary form of ADP-ribosylation of proteins in response to DNA damage. Serine ADP-ribosylation at Ser-11 (H3S10ADPr) is mutually exclusive with phosphorylation at Ser-11 (H3S10ph) and impairs acetylation at Lys-10 (H3K9ac).

Subcellular Location:

Nucleus. Chromosome.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Expressed in testicular cells.

Subunit Structure:

The nucleosome is a histone octamer containing two molecules each of H2A, H2B, H3 and H4 assembled in one H3-H4 heterotetramer and two H2A-H2B heterodimers. The octamer wraps approximately 147 bp of DNA.

Family&Domains:

Belongs to the histone H3 family.

Research Fields

· Human Diseases > Substance dependence > Alcoholism.

· Human Diseases > Cancers: Overview > Transcriptional misregulation in cancer.

· Human Diseases > Immune diseases > Systemic lupus erythematosus.

References

1). P21 and P27 promote tumorigenesis and progression via cell cycle acceleration in seminal vesicles of TRAMP mice. International Journal of Biological Sciences (PubMed: 31592235) [IF=9.2]

2). Triclocarban exposure affects mouse oocyte in vitro maturation through inducing mitochondrial dysfunction and oxidative stress. Environmental Pollution (PubMed: 32135433) [IF=8.9]

Application: IF/ICC    Species: mouse    Sample: oocytes

Fig. 8. TCC exposure altered epigenetic modification in mouse oocytes. (A) Images depicting H3K27me2 in control and TCC-treated oocytes. H3K27me2, red. Bar, 10 mm. (B) The fluorescence intensity of H3K27me2 in control and TCC- treated oocytes. Control, n ¼ 20; 12 mM, n ¼ 19. ***Significantly different (P < 0.0001). (C) Images depicting H3K27me3 in control and TCC-treated oocytes. H3K27me2, red. Bar, 10 mm. (D) The fluorescence intensity of H3K27me3 in control and TCC- treated oocytes. Control, n ¼ 30; 12 mM, n ¼ 24. ***Significantly different (P < 0.0001). (For interpretation of the references to colour in this figure legend, the reader is referred to the Web version of this article.)

3). HBx-mediated decrease of AIM2 contributes to hepatocellular carcinoma metastasis. Molecular Oncology (PubMed: 28580773) [IF=6.6]

Application: WB    Species:    Sample:

Figure 3. EZH2 is required for HBx-induced AIM2 decrease. A. AIM2 mRNA and protein expression levels were tested in Bel-7402 and SMMC-7721 cells treated with 5-Aza for 24 h. B. EZH2 and H3K27me3 level was detected in Bel-7402 and SMMC-7721 cells transfected with HBx. C. EZH2 was transiently knocked down by siRNAs in HBx-expressing Bel-7402 and SMMC-7721 cells. AIM2 expression was determined. D. EZH2 activity was blocked by its inhibitor GSK-126 in cells with or without the presence of HBx. The impact of EZH2 on AIM2 expression was checked.

4). Bisphenol B exposure disrupts mouse oocyte meiotic maturation in vitro through affecting spindle assembly and chromosome alignment. Frontiers in Cell and Developmental Biology (PubMed: 33392205) [IF=5.5]

Application: IF/ICC    Species: mouse    Sample: oocytes

FIGURE 7 | BPB exposure altered epigenetic modification in mouse oocytes.(A) Images depicting H3K27me3 in control and BPB-treated oocytes.H3K27me3, red. Bar, 10 µm.

5). Bisphenol F exposure affects mouse oocyte in vitro maturation through inducing oxidative stress and DNA damage. ENVIRONMENTAL TOXICOLOGY (PubMed: 35218298) [IF=4.5]

6). High-dose synthetic phenolic antioxidant propyl gallate impairs mouse oocyte meiotic maturation through inducing mitochondrial dysfunction and DNA damage. Environmental Toxicology (PubMed: 37052413) [IF=4.5]

7). Effects of Acute Fluorene-9-Bisphenol Exposure on Mouse Oocyte in vitro Maturation and Its Possible Mechanisms. ENVIRONMENTAL AND MOLECULAR MUTAGENESIS (PubMed: 30499614) [IF=2.8]

Application: IF/ICC    Species: mouse    Sample: oocytes

Fig. 6. |BHPF exposure alters H3K9me3 and H3K27me3 levels in mouse oocytes. (A) Immunofluorescent staining for H3K9me3 in control and BHPF-treated oocytes. H3K9me3 level was significantly higher in treated oocytes. Red, H3K9me3; Blue, DAPI. Scale bar, 5 μm.(B) Average H3K9me3 fluorescence intensity in control and BHPF-treated mouse oocytes. (C) Immunofluorescent staining for H3K27me3 in control and BHPF-treated oocytes. H3K27me3 level was significantly higher in treated oocytes. Red, H3K27me3; Blue, DAPI. Scale bar, 5 μm (D) Average H3K27me3 fluorescence intensity in control and BHPF-treated mouse oocytes. **P < 0.01.

8). Isobutylparaben negatively affects porcine oocyte maturation through increasing oxidative stress and cytoskeletal abnormalities. ENVIRONMENTAL AND MOLECULAR MUTAGENESIS (PubMed: 31922297) [IF=2.8]

Application: IF/ICC    Species: Pig    Sample: Cumulus cells

Fig. 6. IBP exposure alters H3K9me3 and H3K27me3 levels in porcine oocytes. (A) Immunofluorescent staining of H3K9me3 and H3K27me3 in control and IBP exposure group. The histone methylation level was significantly higher in treated oocytes. H3K9me3 and H3K27me3; Blue,DAPI. (B) Average H3K9me3 fluorescent intensity of H3K9me3 in control and IBP-treated oocytes. (C) Average fluorescent intensity of H3K27me3 in control and IBP-treated oocytes. **P < 0.01; ***P < 0.001; scale bar = 50 μm. DIC, differential interference contrast.

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