IL18R1 Antibody | Affinity Biosciences

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IL18R1 Antibody - FIGURE 4 Expression of IL18R1 in the muscle tissues and blood of IIMs patients.

Product:	IL18R1 Antibody
Catalog:	DF7059
Description:	Rabbit polyclonal antibody to IL18R1
Application:	WB IHC IF/ICC
Cited expt.:	WB
Reactivity:	Human, Mouse, Rat
Prediction:	Pig
Mol.Wt.:	62kDa(Observed); 62kD(Calculated).
Uniprot:	Q13478
RRID:	AB_2839015

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

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200ul	$350	In stock

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Related Downloads

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:500-1:1000, IHC 1:50-1:100, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user. For optimal experimental results, antibody reuse is not recommended.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse,Rat

Prediction:

Pig(88%)

Clonality:

Polyclonal

Specificity:

IL18R1 Antibody detects endogenous levels of total IL18R1.

RRID:

AB_2839015
Cite Format: Affinity Biosciences Cat# DF7059, RRID:AB_2839015.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

CD218 antigen-like family member A; CD218a; CDw218a; CDw218a antigen; IL 1Rrp; IL-18R-1; IL-18R1; IL-1Rrp; IL1 receptor related protein; IL1 receptor-related protein; IL18R_HUMAN; IL18R1; IL18RA; Il18ralpha; IL1R-rp; IL1RRP; Interleukin 18 receptor 1; Interleukin 18 receptor, alpha chain; Interleukin-18 receptor 1;

Immunogens

Immunogen:

A synthesized peptide derived from human IL18R1, corresponding to a region within the internal amino acids.

Uniprot:

Q13478(IL18R_HUMAN) >>Visit HPA database.

Gene(ID):

IL18R1(8809)

Expression:

Q13478 IL18R_HUMAN:

Highly expressed in leukocytes, spleen, lung. Also expressed, but at lower levels, in liver, small intestine, colon, prostate, thymus, placenta, and heart. Specifically coexpressed with IL18R1 in Th1 cells (PubMed:10925275, PubMed:11046021, PubMed:10653850).

Description:

The protein encoded by this gene is a cytokine receptor that belongs to the interleukin 1 receptor family. This receptor specifically binds interleukin 18 (IL18), and is essential for IL18 mediated signal transduction. IFN-alpha and IL12 are reported to induce the expression of this receptor in NK and T cells. This gene along with four other members of the interleukin 1 receptor family, including IL1R2, IL1R1, ILRL2 (IL-1Rrp2), and IL1RL1 (T1/ST2), form a gene cluster on chromosome 2q. [provided by RefSeq, Jul 2008]

Sequence:

Q13478 IL18R_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MNCRELPLTLWVLISVSTAESCTSRPHITVVEGEPFYLKHCSCSLAHEIETTTKSWYKSSGSQEHVELNPRSSSRIALHDCVLEFWPVELNDTGSYFFQMKNYTQKWKLNVIRRNKHSCFTERQVTSKIVEVKKFFQITCENSYYQTLVNSTSLYKNCKKLLLENNKNPTIKKNAEFEDQGYYSCVHFLHHNGKLFNITKTFNITIVEDRSNIVPVLLGPKLNHVAVELGKNVRLNCSALLNEEDVIYWMFGEENGSDPNIHEEKEMRIMTPEGKWHASKVLRIENIGESNLNVLYNCTVASTGGTDTKSFILVRKADMADIPGHVFTRGMIIAVLILVAVVCLVTVCVIYRVDLVLFYRHLTRRDETLTDGKTYDAFVSYLKECRPENGEEHTFAVEILPRVLEKHFGYKLCIFERDVVPGGAVVDEIHSLIEKSRRLIIVLSKSYMSNEVRYELESGLHEALVERKIKIILIEFTPVTDFTFLPQSLKLLKSHRVLKWKADKSLSYNSRFWKNLLYLMPAKTVKPGRDEPEVLPVLSES

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Pig

Rabbit

Dog

Bovine

Horse

Sheep

Xenopus

Zebrafish

Chicken

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

Research Backgrounds

Q13478_IL18R_HUMAN

Function:

Within the IL18 receptor complex, responsible for the binding of the proinflammatory cytokine IL18, but not IL1A nor IL1B. Involved in IL18-mediated IFNG synthesis from T-helper 1 (Th1) cells. Contributes to IL18-induced cytokine production, either independently of SLC12A3, or as a complex with SLC12A3 (By similarity).

PTMs:

N-glycosylated. N-linked glycosyl chains contribute to ligand recognition and intra-receptor interactions required for formation of an active ternary receptor complex.

Subcellular Location:

Membrane>Single-pass type I membrane protein.

Tissue Specificity:

Family&Domains:

The TIR domain mediates NAD(+) hydrolase (NADase) activity. Self-association of TIR domains is required for NADase activity.

Belongs to the interleukin-1 receptor family.

Research Fields

· Environmental Information Processing > Signaling molecules and interaction > Cytokine-cytokine receptor interaction. (View pathway)

· Environmental Information Processing > Signal transduction > TNF signaling pathway. (View pathway)

· Human Diseases > Immune diseases > Inflammatory bowel disease (IBD).

References

1). The Expression of Cytokine Profiles and Related Receptors in Idiopathic Inflammatory Myopathies. Frontiers in Pharmacology, 2022 (PubMed: 35517781) [IF=5.6]

Application: WB Species: Human Sample: muscle tissues

FIGURE 4 Expression of IL18R1 in the muscle tissues and blood of IIMs patients. (A) The expression of IL18R1 mRNA in the muscle tissues of 19 DM patients and 16 HC. (B) Western blot showed the expression of IL18R1 protein in muscle tissues of 8 DM patients and 5 HC. (C) Flow cytometry analysis the expression of IL18R1 on lymphocytes among 19 untreated IIMs (9 anti-MDA5 positive) patients, 24 treated IIMs patients and 18 HC. Unpaired t-test was used for comparison. Pre-IIMs: IIMs before treatment; Post-IIMs: IIMs treatment.

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IL18R1 Antibody - #DF7059