GFAP Antibody - #BF0345
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| Product: | GFAP Antibody |
| Catalog: | BF0345 |
| Description: | Mouse monoclonal antibody to GFAP |
| Application: | WB IHC-P |
| Reactivity: | Human, Mouse, Rat |
| Mol.Wt.: | 50kDa; 50kD(Calculated). |
| Uniprot: | P14136 |
| RRID: | AB_2833847 |
Product Info
Source:
Mouse
Application:
WB 1:500-1:2000, IHC-p 1:200-1:1000
*The optimal dilutions should be determined by the end user.
*Tips:
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
Reactivity:
Human,Mouse,Rat
Clonality:
Monoclonal [AFB1823]
Specificity:
GFAP antibody detects endogenous levels of total GFAP.
RRID:
AB_2833847
Cite Format: Affinity Biosciences Cat# BF0345, RRID:AB_2833847.
Cite Format: Affinity Biosciences Cat# BF0345, RRID:AB_2833847.
Conjugate:
Unconjugated.
Purification:
Affinity-chromatography.
Storage:
Mouse IgG1 in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:
Fold/Unfold
wu:fb34h11; ALXDRD; cb345; etID36982.3; FLJ42474; FLJ45472; GFAP; GFAP_HUMAN; gfapl; Glial fibrillary acidic protein; Intermediate filament protein; wu:fk42c12; xx:af506734; zgc:110485;
Immunogens
Immunogen:
Purified recombinant fragment of human GFAP expressed in E. Coli.
Uniprot:
Gene(ID):
Expression:
Description:
This gene encodes one of the major intermediate filament proteins of mature astrocytes. It is used as a marker to distinguish astrocytes from other glial cells during development. Mutations in this gene cause Alexander disease, a rare disorder of astrocytes in the central nervous system. An additional transcript variant has been described, but its full length sequence has not been determined.
Sequence:
- P14136 GFAP_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MERRRITSAARRSYVSSGEMMVGGLAPGRRLGPGTRLSLARMPPPLPTRVDFSLAGALNAGFKETRASERAEMMELNDRFASYIEKVRFLEQQNKALAAELNQLRAKEPTKLADVYQAELRELRLRLDQLTANSARLEVERDNLAQDLATVRQKLQDETNLRLEAENNLAAYRQEADEATLARLDLERKIESLEEEIRFLRKIHEEEVRELQEQLARQQVHVELDVAKPDLTAALKEIRTQYEAMASSNMHEAEEWYRSKFADLTDAAARNAELLRQAKHEANDYRRQLQSLTCDLESLRGTNESLERQMREQEERHVREAASYQEALARLEEEGQSLKDEMARHLQEYQDLLNVKLALDIEIATYRKLLEGEENRITIPVQTFSNLQIRETSLDTKSVSEGHLKRNIVVKTVEMRDGEVIKESKQEHKDVM
PTMs - P14136 As Substrate
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| T7 | Phosphorylation | Q96GD4 (AURKB) , P17612 (PRKACA) , Q13464 (ROCK1) | Uniprot |
| S8 | Phosphorylation | P17612 (PRKACA) | Uniprot |
| R11 | Methylation | Uniprot | |
| R12 | Methylation | Uniprot | |
| S13 | Phosphorylation | Q13464 (ROCK1) , P17612 (PRKACA) , Q96GD4 (AURKB) | Uniprot |
| Y14 | Phosphorylation | Uniprot | |
| S17 | Phosphorylation | Uniprot | |
| S38 | Phosphorylation | Q96GD4 (AURKB) , Q13464 (ROCK1) , P17612 (PRKACA) | Uniprot |
| K95 | Acetylation | Uniprot | |
| Y116 | Phosphorylation | Uniprot | |
| T131 | Phosphorylation | Uniprot | |
| T150 | Phosphorylation | Uniprot | |
| K154 | Acetylation | Uniprot | |
| Y172 | Phosphorylation | Uniprot | |
| K189 | Acetylation | Uniprot | |
| K228 | Acetylation | Uniprot | |
| T240 | Phosphorylation | Uniprot | |
| Y242 | Phosphorylation | Uniprot | |
| K260 | Acetylation | Uniprot | |
| S305 | Phosphorylation | Uniprot | |
| Y324 | Phosphorylation | Uniprot | |
| K339 | Acetylation | Uniprot | |
| Y349 | Phosphorylation | Uniprot |
Research Backgrounds
Function:
GFAP, a class-III intermediate filament, is a cell-specific marker that, during the development of the central nervous system, distinguishes astrocytes from other glial cells.
PTMs:
Phosphorylated by PKN1.
Subcellular Location:
Cytoplasm.
Note: Associated with intermediate filaments.
Tissue Specificity:
Expressed in cells lacking fibronectin.
Subunit Structure:
Interacts with SYNM (By similarity). Isoform 3 interacts with PSEN1 (via N-terminus).
Family&Domains:
Belongs to the intermediate filament family.
Research Fields
· Environmental Information Processing > Signal transduction > Jak-STAT signaling pathway. (View pathway)
References
1). Design, synthesis and bioactivity study of N-salicyloyl tryptamine derivatives as multifunctional agents for the treatment of neuroinflammation. EUROPEAN JOURNAL OF MEDICINAL CHEMISTRY
(PubMed: 32182488)
[IF=6.7]
Application: IF/ICC Species: Mouse Sample: BV2 and C6 cells
Fig. 4. Effects of compounds on GFAP and Iba-1 expression in the LPS-induced astrocytes (C6, treated with 10 mg/mL of LPS) and microglia (BV2, treated with 1 mg/mL of LPS). A.
Representative fluorescence microscopy images of GFAP immunostaining (Magnification 20). B. Representative fluorescence microscopy images of Iba-1 immunostaining
(Magnification 40). C. Histograms show relative changes of GFAP expressed as mean ± SD of the three independent experiments (n ¼ 3). Ctrl. ¼ Control, LPS ¼ Lipopolysaccharide
(10 mg/mL); 3, 13, 16 ¼ treatments by compounds 3, 13, 16 (20 mM) along with LPS (10 mg/mL). D. Histograms show relative changes of Iba-1 expressed as mean ± SD of the three
independent experiments (n ¼ 3). All the data were analyzed using Image Pro Plus and expressed as a percent of LPS group values (fluorescence intensity). (#) Significant difference
(##p < 0.01, ###p < 0.001) vs. control and (*) significant difference (*p < 0.05 and **p < 0.01) vs. LPS.
2). LY294002 alleviates bone cancer pain by reducing mitochondrial dysfunction and the inflammatory response. International Journal of Molecular Medicine
(PubMed: 37026522)
[IF=5.4]
3). Carbenoxolone has the potential to ameliorate acute incision pain in rats. Molecular Medicine Reports
(PubMed: 34013377)
[IF=3.4]
Application: IF/ICC Species: rat Sample:
Figure 3. |Co‑localization of Px1 and GFAP in IP model rats. Px1 (green) and GFAP (red)expression was analyzed using an immunofluorescence assay.Magnification, x200; scale bar, 10 µm. C,control group; IP, incision pain; CBX, carbenoxolone; 10panx, pannexin‑1 mimetic inhibitory peptide; Px1, pannexin 1;GFAP, glial fibrillary acidic protein
Application: IF/ICC Species: Sample:
Figure 3. |Co‑localization of Px1 and GFAP in IP model rats. Px1 (green) and GFAP (red) expression was analyzed using an immunofluorescence assay. Magnification, x200; scale bar, 10 µm. C, control group; IP, incision pain; CBX, carbenoxolone; 10panx, pannexin‑1 mimetic inhibitory peptide; Px1, pannexin 1;GFAP, glial fibrillary acidic protein.
4). Emodin inhibits HDAC6 mediated NLRP3 signaling and relieves chronic inflammatory pain in mice. Experimental and therapeutic medicine
(PubMed: 38144917)
[IF=2.7]
5). Electroacupuncture prevents the development or establishment of chronic pain via IL-33/ST2 signaling in hyperalgesic priming model rats. Neuroscience letters
(PubMed: 38142925)
[IF=2.5]
6). The effect of AQP4 on tau protein aggregation in neurodegeneration and persistent neuroinflammation after cerebral microinfarcts. Open medicine (Warsaw, Poland)
(PubMed: 37873537)
[IF=2.1]
Application: IF/ICC Species: Mouse Sample:
Figure 1 Effects of AQP4 on GFAP and p-tau aggregation. (a and b) Histology, qPCR, and Western blot assays verify adenovirus efficiency; (c and d) GFAP and AQP4 and p-tau aggregation (pSer202/pThr205, p-tau) and neuronal marker (NeuN) co-expression were evaluated by Immunofluorescence double staining. *p < 0.05 vs control. All experiments were repeated 3 times. Each group has 7 mice.
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