B Raf Antibody - Internal - DF7134
-
- Product Name
- B Raf Antibody
-
- Catalog No.
- DF7134
-
- RRID
- AB_2839088
-
- Source
- Rabbit
-
- Application
- WB,IHC,ELISA(peptide)
-
- Reactivity
- Human, Mouse, Rat
-
- Prediction
- Pig(100%), Zebrafish(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(100%), Xenopus(100%)
-
- UniProt
-
- Mol.Wt
- 84kD;
84kDa(Calculated). -
- Concentration
- 1mg/ml
Related Products
Product Information
Alternative Names:Expand▼
FLJ95109; 94 kDa B raf protein; B raf 1; B Raf proto oncogene serine threonine protein kinase; B Raf proto oncogene, serine/threonine kinase; B RAF1; B-Raf proto-oncogene serine/threonine-protein kinase (p94); BRAF 1; BRAF; BRAF_HUMAN; BRAF1; cRmil; MGC126806; MGC138284; Murine sarcoma viral (v-raf) oncogene homolog B1; Murine sarcoma viral v raf oncogene homolog B1; NS7; Oncogen BRAF; oncogene BRAF1; p94; Proto-oncogene B-Raf; Proto-oncogene c-Rmil; RAFB 1; RAFB1; RMIL; Serine/threonine-protein kinase B-raf; v raf murine sarcoma viral oncogene homolog B; v raf murine sarcoma viral oncogene homolog B1; v-Raf murine sarcoma viral oncogene homolog B1;
Applications:
WB 1:500-1:2000, IHC 1:50-1:200, ELISA(peptide) 1:20000-1:40000
*The optimal dilutions should be determined by the end user.
Reactivity:
Human, Mouse, Rat
Predicted Reactivity:
Pig(100%), Zebrafish(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(100%), Xenopus(100%)
Source:
Rabbit
Clonality:
Polyclonal
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Specificity:
B Raf Antibody detects endogenous levels of total B Raf.
RRID:
AB_2839088
Please cite this product as: Affinity Biosciences Cat# DF7134, RRID:AB_2839088.
Format:
Liquid
Concentration:
1mg/ml
Storage Condition and Buffer:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt.
Immunogen Information
Immunogen:
A synthesized peptide derived from human B Raf, corresponding to a region within the internal amino acids.
Uniprot:
>>Visit The Human Protein Atlas
Gene ID:
Gene Name:
BRAF
Molecular Weight:
Observed Mol.Wt.: 84kD.
Predicted Mol.Wt.: 84kDa(Calculated)..
Subcellular Location:
Nucleus. Cytoplasm. Cell membrane. Colocalizes with RGS14 and RAF1 in both the cytoplasm and membranes.
Tissue Specificity:
P15056 BRAF_HUMAN:
Brain and testis.
Description:
BRAF: v-raf murine sarcoma viral oncogene homolog B1, also known as BRAF1; RAFB1; B-RAF1; FLJ95109. Entrez Protein NP_004324. It is the main effectors recruited by GTP-bound Ras to activate the MEK-MAP kinase pathway. B-Raf contains three consensus Akt phosphorylationsites (Ser364, Ser428, and Thr439). B-Raf is a key regulatory molecule of the mitogen-activated protein kinase kinase (MEK), it has a long amino-terminal region,the region is essential for homo-dimerization of B-Raf and hetero-dimerization of B-Raf and c-Raf at the plasma membrane, followed by phosphorylation of Thr118 in the amino-terminal B-Raf-specific region. Notably, in calcium ionophore-stimulated HeLa cells, B-Raf could propagate signals to MEK under the basal level of GTP-Ras. Expression of Raf-B is highly restricted with highestlevels in the cerebrum and testes and defects in braf are involved in a wide range of cancers. The BRAF gene mutation is frequently detected in papillary thyroid carcinoma,melanocytic nevi, primary cutaneous melanomas and colorectal cancers.
Sequence:
- P15056 BRAF_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MAALSGGGGGGAEPGQALFNGDMEPEAGAGAGAAASSAADPAIPEEVWNIKQMIKLTQEHIEALLDKFGGEHNPPSIYLEAYEEYTSKLDALQQREQQLLESLGNGTDFSVSSSASMDTVTSSSSSSLSVLPSSLSVFQNPTDVARSNPKSPQKPIVRVFLPNKQRTVVPARCGVTVRDSLKKALMMRGLIPECCAVYRIQDGEKKPIGWDTDISWLTGEELHVEVLENVPLTTHNFVRKTFFTLAFCDFCRKLLFQGFRCQTCGYKFHQRCSTEVPLMCVNYDQLDLLFVSKFFEHHPIPQEEASLAETALTSGSSPSAPASDSIGPQILTSPSPSKSIPIPQPFRPADEDHRNQFGQRDRSSSAPNVHINTIEPVNIDDLIRDQGFRGDGGSTTGLSATPPASLPGSLTNVKALQKSPGPQRERKSSSSSEDRNRMKTLGRRDSSDDWEIPDGQITVGQRIGSGSFGTVYKGKWHGDVAVKMLNVTAPTPQQLQAFKNEVGVLRKTRHVNILLFMGYSTKPQLAIVTQWCEGSSLYHHLHIIETKFEMIKLIDIARQTAQGMDYLHAKSIIHRDLKSNNIFLHEDLTVKIGDFGLATVKSRWSGSHQFEQLSGSILWMAPEVIRMQDKNPYSFQSDVYAFGIVLYELMTGQLPYSNINNRDQIIFMVGRGYLSPDLSKVRSNCPKAMKRLMAECLKKKRDERPLFPQILASIELLARSLPKIHRSASEPSLNRAGFQTEDFSLYACASPKTPIQAGGYGAFPVH
Research Background
Function:
Protein kinase involved in the transduction of mitogenic signals from the cell membrane to the nucleus (Probable). Phosphorylates MAP2K1, and thereby activates the MAP kinase signal transduction pathway. May play a role in the postsynaptic responses of hippocampal neurons.
Post-translational Modifications:
Phosphorylation at Ser-365 by SGK1 inhibits its activity.
Methylation at Arg-671 decreases stability and kinase activity.
Ubiquitinated by RNF149; which leads to proteasomal degradation. Polyubiquitinated at Lys-578 in response to EGF.
Subcellular Location:
Nucleus. Cytoplasm. Cell membrane.
Note: Colocalizes with RGS14 and RAF1 in both the cytoplasm and membranes.
Tissue Specificity:
Brain and testis.
Subunit Structure:
Monomer. Homodimer. Heterodimerizes with RAF1, and the heterodimer possesses a highly increased kinase activity compared to the respective homodimers or monomers. Heterodimerization is mitogen-regulated and enhanced by 14-3-3 proteins. MAPK1/ERK2 activation can induce a negative feedback that promotes the dissociation of the heterodimer by phosphorylating BRAF at Thr-753. Heterodimerizes (via N-terminus) with KSR1 (via N-terminus) or KSR2 (via N-terminus) in a MAP2K1-dependent manner. Interacts with MAP2K1 and MAP2K2. Found in a complex with at least BRAF, HRAS, MAP2K1, MAPK3 and RGS14. Interacts with RIT1. Interacts (via N-terminus) with RGS14 (via RBD domains); the interaction mediates the formation of a ternary complex with RAF1, a ternary complex inhibited by GNAI1 (By similarity). Interacts with DGKH. Interacts with PRMT5. Interacts with KSR2. Interacts with AKAP13, MAP2K1 and KSR1. Identified in a complex with AKAP13, MAP2K1 and KSR1. Interacts with FNIP1 and FNIP2.
Similarity:
Belongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family. RAF subfamily.
Research Fields
Research Fields:
· Cellular Processes > Cellular community - eukaryotes > Focal adhesion.(View pathway)
· Cellular Processes > Cell motility > Regulation of actin cytoskeleton.(View pathway)
· Environmental Information Processing > Signal transduction > ErbB signaling pathway.(View pathway)
· Environmental Information Processing > Signal transduction > FoxO signaling pathway.(View pathway)
· Environmental Information Processing > Signal transduction > mTOR signaling pathway.(View pathway)
· Environmental Information Processing > Signal transduction > Rap1 signaling pathway.(View pathway)
· Environmental Information Processing > Signal transduction > cAMP signaling pathway.(View pathway)
· Environmental Information Processing > Signal transduction > MAPK signaling pathway.(View pathway)
· Human Diseases > Cancers: Specific types > Glioma.(View pathway)
· Human Diseases > Cancers: Specific types > Prostate cancer.(View pathway)
· Human Diseases > Cancers: Specific types > Colorectal cancer.(View pathway)
· Human Diseases > Cancers: Overview > Proteoglycans in cancer.
· Human Diseases > Cancers: Specific types > Hepatocellular carcinoma.(View pathway)
· Human Diseases > Cancers: Specific types > Acute myeloid leukemia.(View pathway)
· Human Diseases > Cancers: Specific types > Endometrial cancer.(View pathway)
· Human Diseases > Cancers: Specific types > Breast cancer.(View pathway)
· Human Diseases > Cancers: Specific types > Chronic myeloid leukemia.(View pathway)
· Human Diseases > Infectious diseases: Viral > Hepatitis C.
· Human Diseases > Cancers: Specific types > Melanoma.(View pathway)
· Human Diseases > Drug resistance: Antineoplastic > EGFR tyrosine kinase inhibitor resistance.
· Human Diseases > Substance dependence > Alcoholism.
· Human Diseases > Drug resistance: Antineoplastic > Endocrine resistance.
· Human Diseases > Cancers: Specific types > Non-small cell lung cancer.(View pathway)
· Human Diseases > Cancers: Specific types > Gastric cancer.(View pathway)
· Human Diseases > Cancers: Overview > Pathways in cancer.(View pathway)
· Human Diseases > Cancers: Specific types > Bladder cancer.(View pathway)
· Human Diseases > Cancers: Specific types > Thyroid cancer.(View pathway)
· Human Diseases > Cancers: Specific types > Renal cell carcinoma.(View pathway)
· Human Diseases > Cancers: Specific types > Pancreatic cancer.(View pathway)
· Organismal Systems > Immune system > Chemokine signaling pathway.(View pathway)
· Organismal Systems > Nervous system > Serotonergic synapse.
· Organismal Systems > Nervous system > Long-term depression.
· Organismal Systems > Circulatory system > Vascular smooth muscle contraction.(View pathway)
· Organismal Systems > Nervous system > Neurotrophin signaling pathway.(View pathway)
· Organismal Systems > Nervous system > Long-term potentiation.
· Organismal Systems > Endocrine system > Insulin signaling pathway.(View pathway)
· Organismal Systems > Immune system > Natural killer cell mediated cytotoxicity.(View pathway)
· Organismal Systems > Endocrine system > Progesterone-mediated oocyte maturation.
Protocols & Troubleshooting
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Catalog Number :
DF7134-BP
(Blocking peptide available as DF7134-BP)
Price/Size :
$350/1mg.
Tips: For phospho antibody, we provide phospho peptide（0.5mg) and non-phospho peptide(0.5mg).
Function :
Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. This mechanism is useful when non-specific binding is an issue, for example, in Western blotting (immunoblot) and immunohistochemistry (IHC). By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific; Specific binding will be absent from the western blot or immunostaining performed with the neutralized antibody.
Format and storage :
Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 10 mg/ml.The purity is >90%,tested by HPLC and MS.Storage Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C.
Precautions :
This product is for research use only. Not for use in diagnostic or therapeutic procedures.
P15056 as Substrate
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| S76 | Phosphorylation | Uniprot | |
| T119 | Phosphorylation | Uniprot | |
| S147 | Phosphorylation | P15056 (BRAF) | Uniprot |
| S151 | Phosphorylation | P28482 (MAPK1) , P15056 (BRAF) , P27361 (MAPK3) | Uniprot |
| K164 | Ubiquitination | Uniprot | |
| T244 | Phosphorylation | Uniprot | |
| K253 | Acetylation | Uniprot | |
| K253 | Ubiquitination | Uniprot | |
| S314 | Phosphorylation | Uniprot | |
| S316 | Phosphorylation | Uniprot | |
| S317 | Phosphorylation | Uniprot | |
| S319 | Phosphorylation | Uniprot | |
| S333 | Phosphorylation | Uniprot | |
| S335 | Phosphorylation | Uniprot | |
| S339 | Phosphorylation | Uniprot | |
| S363 | Phosphorylation | Uniprot | |
| S364 | Phosphorylation | P31751 (AKT2) , Q9Y243 (AKT3) , O00141 (SGK1) | Uniprot |
| S365 | Phosphorylation | O00141 (SGK1) , P31749 (AKT1) , Q9Y243 (AKT3) | Uniprot |
| T373 | Phosphorylation | P15056 (BRAF) | Uniprot |
| S394 | Phosphorylation | Uniprot | |
| T396 | Phosphorylation | Uniprot | |
| S399 | Phosphorylation | Uniprot | |
| T401 | Phosphorylation | P28482 (MAPK1) , P27361 (MAPK3) | Uniprot |
| S405 | Phosphorylation | Uniprot | |
| K418 | Acetylation | Uniprot | |
| S419 | Phosphorylation | P15056 (BRAF) | Uniprot |
| S428 | Phosphorylation | Q9Y243 (AKT3) , P31751 (AKT2) | Uniprot |
| S429 | Phosphorylation | Q9Y243 (AKT3) , P31749 (AKT1) | Uniprot |
| S430 | Phosphorylation | P17612 (PRKACA) | Uniprot |
| S431 | Phosphorylation | Uniprot | |
| S432 | Phosphorylation | Uniprot | |
| T440 | Phosphorylation | P31751 (AKT2) | Uniprot |
| S445 | Phosphorylation | Uniprot | |
| S446 | Phosphorylation | Q13153 (PAK1) , Q15382 (RHEB) | Uniprot |
| S447 | Phosphorylation | Uniprot | |
| T458 | Phosphorylation | Uniprot | |
| S465 | Phosphorylation | Uniprot | |
| S467 | Phosphorylation | Uniprot | |
| T470 | Phosphorylation | Uniprot | |
| K473 | Ubiquitination | Uniprot | |
| K475 | Ubiquitination | Uniprot | |
| Y566 | Phosphorylation | Uniprot | |
| K578 | Ubiquitination | Uniprot | |
| S579 | Phosphorylation | Uniprot | |
| T599 | Phosphorylation | Uniprot | |
| K601 | Ubiquitination | Uniprot | |
| S602 | Phosphorylation | Uniprot | |
| S605 | Phosphorylation | Uniprot | |
| S614 | Phosphorylation | P15056 (BRAF) | Uniprot |
| R671 | Methylation | Uniprot | |
| S675 | Phosphorylation | Uniprot | |
| K687 | Ubiquitination | Uniprot | |
| S727 | Phosphorylation | Uniprot | |
| S729 | Phosphorylation | Q13131 (PRKAA1) | Uniprot |
| S732 | Phosphorylation | Uniprot | |
| Y746 | Phosphorylation | Uniprot | |
| S750 | Phosphorylation | P28482 (MAPK1) , P15056 (BRAF) | Uniprot |
| T753 | Phosphorylation | P28482 (MAPK1) , P27361 (MAPK3) | Uniprot |
| Y760 | Phosphorylation | Uniprot |
P15056 as PTM Enzyme
| Substrate | Site | Source |
|---|---|---|
| P15056 (BRAF) | S147 | Uniprot |
| P15056 (BRAF) | S151 | Uniprot |
| P15056 (BRAF) | T373 | Uniprot |
| P15056 (BRAF) | S419 | Uniprot |
| P15056 (BRAF) | S614 | Uniprot |
| P15056 (BRAF) | S750 | Uniprot |
| P33981 (TTK) | S281 | Uniprot |
| P33981 (TTK) | S436 | Uniprot |
| P36507 (MAP2K2) | S222 | Uniprot |
| P36507 (MAP2K2) | S226 | Uniprot |
| P68104 (EEF1A1) | S21 | Uniprot |
| P68104 (EEF1A1) | T88 | Uniprot |
| Q02750 (MAP2K1) | S218 | Uniprot |
| Q02750-1 (MAP2K1) | S222 | Uniprot |
| Q05639 (EEF1A2) | S21 | Uniprot |
| Q92934 (BAD) | S75 | Uniprot |
| Q92934 (BAD) | S99 | Uniprot |
| Q92934 (BAD) | S118 | Uniprot |
| Q92934 (BAD) | S134 | Uniprot |
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