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$350 200ul

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  • Product Name
    RRM2 Antibody
  • Catalog No.
    DF7248
  • RRID
    AB_2839187
  • Source
    Rabbit
  • Application
    WB,IHC,ELISA(peptide)
  • Reactivity
    Human, Mouse, Rat
  • Prediction
    Bovine, Horse, Chicken
  • UniProt
  • Mol.Wt
    45kD;
    45kDa(Calculated).
  • Concentration
    1mg/ml
  • Browse similar products>>

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Product Information

Alternative Names:Expand▼

cb111; chunp6884; etID309896.20; R2; reductase M2 polypeptide variant 1; reductase M2 polypeptide variant 2; reductase M2 polypeptide variant 3a; reductase M2 polypeptide variant 3b; reductase M2 polypeptide variant 3c; reductase M2 polypeptide variant 3d; Ribonucleoside-diphosphate reductase subunit M2; Ribonucleotide reductase M2; Ribonucleotide reductase M2 polypeptide; Ribonucleotide reductase M2 subunit; ribonucleotide reductase protein r2 class I; Ribonucleotide reductase regulatory subunit M2; Ribonucleotide reductase small chain; Ribonucleotide reductase small subunit; Ribonucleotide reductase, R2 subunit; RIR2_HUMAN; RR2; RR2M; RRM2;

Applications:

WB 1:500-1:2000, IHC 1:50-1:200, ELISA(peptide) 1:20000-1:40000
*The optimal dilutions should be determined by the end user.

Reactivity:

Human, Mouse, Rat

Predicted Reactivity:

Bovine, Horse, Chicken

Source:

Rabbit

Clonality:

Polyclonal

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Specificity:

RRM2 Antibody detects endogenous levels of total RRM2.

RRID:

AB_2839187
Please cite this product as: Affinity Biosciences Cat# DF7248, RRID:AB_2839187.

Format:

Liquid

Concentration:

1mg/ml

Storage Condition and Buffer:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt.

Immunogen Information in 3D

Immunogen:

A synthesized peptide derived from human RRM2, corresponding to a region within C-terminal amino acids.

Uniprot:



>>Visit The Human Protein Atlas

Gene ID:

Gene Name:

RRM2

Molecular Weight:

Observed Mol.Wt.: 45kD.
Predicted Mol.Wt.: 45kDa(Calculated)..

Subcellular Location:

Cytoplasm.

Description:

Ribonucleotide reductase M2 subunit is one of two subunits that constitute ribonucleotide reductase, the enzyme that catalyzes the conversion of ribonucleotide 5'-diphosphates into 2'-deoxyribonucleotides, a rate-limiting step in the production of 2'-deoxyribonucleoside 5'-diphosphates (dNTP) required for DNA synthesis and repair that is required for DNA synthesis and repair [PMID:20825972, 19250552]. RRM2 is only expressed during the late G1/early S phase, and degraded in late S phase, and the activity of RNR, and therefore DNA synthesis and cell proliferation, is controlled during the cell cycle by the synthesis and degradation of RRM2 subunit.

Sequence:
MLSLRVPLAPITDPQQLQLSPLKGLSLVDKENTPPALSGTRVLASKTARRIFQEPTEPKTKAAAPGVEDEPLLRENPRRFVIFPIEYHDIWQMYKKAEASFWTAEEVDLSKDIQHWESLKPEERYFISHVLAFFAASDGIVNENLVERFSQEVQITEARCFYGFQIAMENIHSEMYSLLIDTYIKDPKEREFLFNAIETMPCVKKKADWALRWIGDKEATYGERVVAFAAVEGIFFSGSFASIFWLKKRGLMPGLTFSNELISRDEGLHCDFACLMFKHLVHKPSEERVREIIINAVRIEQEFLTEALPVKLIGMNCTLMKQYIEFVADRLMLELGFSKVFRVENPFDFMENISLEGKTNFFEKRVGEYQRMGVMSSPTENSFTLDADF

Research Background

Function:

Provides the precursors necessary for DNA synthesis. Catalyzes the biosynthesis of deoxyribonucleotides from the corresponding ribonucleotides. Inhibits Wnt signaling.

Post-translational Modifications:

Phosphorylation on Ser-20 relieves the inhibitory effect on Wnt signaling.

Subcellular Location:

Cytoplasm.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte

Subunit Structure:

Heterodimer of a large and a small subunit.

Similarity:

Belongs to the ribonucleoside diphosphate reductase small chain family.

Research Fields

Research Fields:

· Cellular Processes > Cell growth and death > p53 signaling pathway.(View pathway)
· Metabolism > Nucleotide metabolism > Purine metabolism.
· Metabolism > Nucleotide metabolism > Pyrimidine metabolism.
· Metabolism > Metabolism of other amino acids > Glutathione metabolism.
· Metabolism > Xenobiotics biodegradation and metabolism > Drug metabolism - other enzymes.
· Metabolism > Global and overview maps > Metabolic pathways.

Reference Citations:

1). Wang Y;Liang F;Zhou Y;Qiu J;Lv Q;Du Z; et al. Sharp Downregulation of Hub Genes Associated With the Pathogenesis of Breast Cancer From Ductal Carcinoma In Situ to Invasive Ductal Carcinoma. Front Oncol 2021 May 21;11:634569. (PubMed: 34094915) [IF=4.848]

2). Xia G et al. Gambogic acid sensitizes gemcitabine efficacy in pancreatic cancer by reducing the expression of ribonucleotide reductase subunit-M2 (RRM2). J Exp Clin Cancer Res 2017 Aug 10;36(1):107 (PubMed: 28797284)

Application: WB    Species:human;    Sample:Not available


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Catalog Number :

DF7248-BP
(Blocking peptide available as DF7248-BP)

Price/Size :

$350/1mg.
Tips: For modified antibodies, we provide modified peptides(0.5mg) and non-modified peptides(0.5mg).

Function :

Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. This mechanism is useful when non-specific binding is an issue, for example, in Western blotting (immunoblot) and immunohistochemistry (IHC). By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific; Specific binding will be absent from the western blot or immunostaining performed with the neutralized antibody.

Format and storage :

Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 10 mg/ml.The purity is >90%,tested by HPLC and MS.Storage Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C.

Precautions :

This product is for research use only. Not for use in diagnostic or therapeutic procedures.

Horse
100%
Bovine
100%
Chicken
83%
Zebrafish
67%
Pig
0%
Sheep
0%
Dog
0%
Xenopus
0%
Rabbit
0%
High similarity Medium similarity Low similarity No similarity
P31350 as Substrate
Site PTM Type Enzyme
T12 Phosphorylation
S20 Phosphorylation P06493 (CDK1) , P24941 (CDK2)
K23 Ubiquitination
S26 Phosphorylation
K30 Ubiquitination
T33 Phosphorylation P06493 (CDK1) , P24941 (CDK2)
S38 Phosphorylation
T40 Phosphorylation
S45 Phosphorylation
K46 Ubiquitination
T47 Phosphorylation
T56 Phosphorylation
K59 Ubiquitination
K61 Ubiquitination
K120 Ubiquitination
K204 Ubiquitination
K205 Ubiquitination
K206 Ubiquitination
R212 Methylation
K217 Ubiquitination
Y221 Phosphorylation
S263 Phosphorylation
K278 Ubiquitination
K283 Ubiquitination
K311 Ubiquitination
T318 Phosphorylation
K358 Ubiquitination
K364 Acetylation
K364 Ubiquitination
Y369 Phosphorylation
S377 Phosphorylation
IMPORTANT: For western blots, incubate membrane with diluted antibody in 5% w/v milk , 1X TBS, 0.1% Tween®20 at 4°C with gentle shaking, overnight.

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