AIMP1 Antibody | Affinity Biosciences

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Western blot analysis of extracts from Hela cells, using AIMP1 Antibody.

DF7261 at 1/100 staining Human spleen tissue by IHC-P.

Product:	AIMP1 Antibody
Catalog:	DF7261
Description:	Rabbit polyclonal antibody to AIMP1
Application:	WB IHC IF/ICC IP FACS
Reactivity:	Human, Mouse, Rat
Prediction:	Pig, Bovine, Horse, Dog
Mol.Wt.:	34kDa; 34kD(Calculated).
Uniprot:	Q12904
RRID:	AB_2839200

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$280	In stock
200ul	$350	In stock

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Related Downloads

MS Report

HPLC Report

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse,Rat

Prediction:

Pig(90%), Bovine(80%), Horse(80%), Dog(90%)

Clonality:

Polyclonal

Specificity:

AIMP1 Antibody detects endogenous levels of total AIMP1.

RRID:

AB_2839200
Cite Format: Affinity Biosciences Cat# DF7261, RRID:AB_2839200.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

AIMP 1; Aimp1; AIMP1/p43; AIMP1_HUMAN; Aminoacyl tRNA synthetase complex-interacting multifunctional protein 1; ARS interacting multifunctional protein 1; EMAP 2; EMAP-2; EMAP-II; EMAP2; EMAPII; Endothelial monocyte activating polypeptide 2; Endothelial monocyte activating polypeptide; Endothelial monocyte-activating polypeptide 2; Endothelial monocyte-activating polypeptide II; HLD3; Multisynthase complex auxiliary component p43; Multisynthetase complex auxiliary component p43; OTTHUMP00000219673; p43; Scye 1; Scye1; Scye1, formerly; Small inducible cytokine subfamily E member 1; Small inducible cytokine subfamily E, member 1 (endothelial monocyte-activating); Small inducible cytokine subfamily E, member 1, formerly;

Immunogens

Immunogen:

Uniprot:

Q12904(AIMP1_HUMAN) >>Visit HPA database.

Gene(ID):

AIMP1(9255)

Description:

The protein encoded by this gene is a cytokine that is specifically induced by apoptosis, and it is involved in the control of angiogenesis, inflammation, and wound healing. The release of this cytokine renders the tumor-associated vasculature sensitive to tumor necrosis factor. The precursor protein is identical to the p43 subunit, which is associated with the multi-tRNA synthetase complex, and it modulates aminoacylation activity of tRNA synthetase in normal cells. This protein is also involved in the stimulation of inflammatory responses after proteolytic cleavage in tumor cells. Multiple transcript variants encoding different isoforms have been found for this gene. A pseudogene has been identified on chromosome 20.

Sequence:

Q12904 AIMP1_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MANNDAVLKRLEQKGAEADQIIEYLKQQVSLLKEKAILQATLREEKKLRVENAKLKKEIEELKQELIQAEIQNGVKQIPFPSGTPLHANSMVSENVIQSTAVTTVSSGTKEQIKGGTGDEKKAKEKIEKKGEKKEKKQQSIAGSADSKPIDVSRLDLRIGCIITARKHPDADSLYVEEVDVGEIAPRTVVSGLVNHVPLEQMQNRMVILLCNLKPAKMRGVLSQAMVMCASSPEKIEILAPPNGSVPGDRITFDAFPGEPDKELNPKKKIWEQIQPDLHTNDECVATYKGVPFEVKGKGVCRAQTMSNSGIK

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Pig

Dog

Horse

Bovine

Chicken

Rabbit

Sheep

Xenopus

Zebrafish

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q12904 As Substrate

Q12904

Site	PTM Type	Enzyme	Source
A2	Acetylation		Uniprot
K9	Ubiquitination		Uniprot
Y24	Phosphorylation		Uniprot
K26	Ubiquitination		Uniprot
K33	Acetylation		Uniprot
K33	Ubiquitination		Uniprot
K35	Ubiquitination		Uniprot
T41	Phosphorylation		Uniprot
K63	Acetylation		Uniprot
K63	Ubiquitination		Uniprot
S90	Phosphorylation		Uniprot
K137	Sumoylation		Uniprot
S140	Phosphorylation	P45983 (MAPK8)	Uniprot
S144	Phosphorylation		Uniprot
C161	S-Nitrosylation		Uniprot
T164	Phosphorylation	P45983 (MAPK8)	Uniprot
S173	Phosphorylation		Uniprot
Y175	Phosphorylation		Uniprot
R187	Methylation		Uniprot
R205	Methylation		Uniprot
S223	Phosphorylation		Uniprot
R250	Methylation		Uniprot
K262	Acetylation		Uniprot
K267	Acetylation		Uniprot
K269	Ubiquitination		Uniprot
K312	Ubiquitination		Uniprot

Research Backgrounds

Q12904_AIMP1_HUMAN

Function:

Non-catalytic component of the multisynthase complex. Stimulates the catalytic activity of cytoplasmic arginyl-tRNA synthase. Binds tRNA. Possesses inflammatory cytokine activity. Negatively regulates TGF-beta signaling through stabilization of SMURF2 by binding to SMURF2 and inhibiting its SMAD7-mediated degradation (By similarity). Involved in glucose homeostasis through induction of glucagon secretion at low glucose levels (By similarity). Promotes dermal fibroblast proliferation and wound repair. Regulates KDELR1-mediated retention of HSP90B1/gp96 in the endoplasmic reticulum (By similarity). Plays a role in angiogenesis by inducing endothelial cell migration at low concentrations and endothelian cell apoptosis at high concentrations. Induces maturation of dendritic cells and monocyte cell adhesion. Modulates endothelial cell responses by degrading HIF-1A through interaction with PSMA7.

PTMs:

Cleaved by caspase-7 in response to apoptosis to produce EMAP-II.

Subcellular Location:

Nucleus. Cytoplasm>Cytosol. Secreted. Endoplasmic reticulum. Golgi apparatus.
Note: Enriched in secretory vesicles of pancreatic alpha cells and secreted from the pancreas in response to low glucose levels (By similarity). Secreted in response to hypoxia (PubMed:10850427). Also secreted in response to both apoptotic and necrotic cell death.

Subunit Structure:

Homodimer. Part of the multisynthetase complex (MSC), a multisubunit complex that groups tRNA ligases for Arg (RARS1), Asp (DARS1), Gln (QARS1), Ile (IARS1), Leu (LARS1), Lys (KARS1), Met (MARS1) the bifunctional ligase for Glu and Pro (EPRS1) and the auxiliary subunits AIMP1/p43, AIMP2/p38 and EEF1E1/p18. Interacts (via N-terminus) with RARS1 (via N-terminus). Part of a complex composed of RARS1, QARS1 and AIMP1. Interacts (via C-terminus) with SMURF2. Interacts (via N-terminus) with HSP90B1/gp96 (via C-terminus) (By similarity). Interacts with PSMA7. Interacts with TARS3.

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AIMP1 Antibody - #DF7261