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$280 100ul
$350 200ul

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  • Product Name
    GCLM Antibody
  • Catalog No.
    DF7268
  • RRID
    AB_2839207
  • Source
    Rabbit
  • Application
    WB,IHC,ELISA(peptide)
  • Reactivity
    Human, Mouse, Rat, Pig
  • Prediction
    Pig, Bovine, Rabbit
  • UniProt
  • Mol.Wt
    30kD;
    31kDa(Calculated).
  • Concentration
    1mg/ml
  • Browse similar products>>

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Product Information

Alternative Names:Expand▼

Gamma ECS regulatory subunit; Gamma-ECS regulatory subunit; Gamma-glutamylcysteine synthetase regulatory subunit; GCLM; GCS light chain; GLCLR; Glutamate cysteine ligase regulatory subunit; Glutamate--cysteine ligase modifier subunit; Glutamate--cysteine ligase regulatory subunit; GSC light chain; GSH0_HUMAN;

Applications:

WB 1:500-1:2000, IHC 1:50-1:200, ELISA(peptide) 1:20000-1:40000
*The optimal dilutions should be determined by the end user.

Reactivity:

Human, Mouse, Rat, Pig

Predicted Reactivity:

Pig, Bovine, Rabbit

Source:

Rabbit

Clonality:

Polyclonal

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Specificity:

GCLM Antibody detects endogenous levels of total GCLM.

RRID:

AB_2839207
Please cite this product as: Affinity Biosciences Cat# DF7268, RRID:AB_2839207.

Format:

Liquid

Concentration:

1mg/ml

Storage Condition and Buffer:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt.

Immunogen Information in 3D

Immunogen:

A synthesized peptide derived from human GCLM, corresponding to a region within N-terminal amino acids.

Uniprot:



>>Visit The Human Protein Atlas

Gene ID:

Gene Name:

GCLM

Molecular Weight:

Observed Mol.Wt.: 30kD.
Predicted Mol.Wt.: 31kDa(Calculated)..

Tissue Specificity:

P48507 GSH0_HUMAN:
In all tissues examined. Highest levels in skeletal muscle.

Description:

Glutamate-cysteine ligase, also known as gamma-glutamylcysteine synthetase, is the first rate limiting enzyme of glutathione synthesis. The enzyme consists of two subunits, a heavy catalytic subunit and a light regulatory subunit. Gamma glutamylcysteine synthetase deficiency has been implicated in some forms of hemolytic anemia.

Sequence:
MGTDSRAAKALLARARTLHLQTGNLLNWGRLRKKCPSTHSEELHDCIQKTLNEWSSQINPDLVREFPDVLECTVSHAVEKINPDEREEMKVSAKLFIVESNSSSSTRSAVDMACSVLGVAQLDSVIIASPPIEDGVNLSLEHLQPYWEELENLVQSKKIVAIGTSDLDKTQLEQLYQWAQVKPNSNQVNLASCCVMPPDLTAFAKQFDIQLLTHNDPKELLSEASFQEALQESIPDIQAHEWVPLWLLRYSVIVKSRGIIKSKGYILQAKRRGS

Research Background

Tissue Specificity:

In all tissues examined. Highest levels in skeletal muscle.

Subunit Structure:

Heterodimer of a catalytic heavy chain and a regulatory light chain.

Similarity:

Belongs to the aldo/keto reductase family. Glutamate--cysteine ligase light chain subfamily.

Research Fields

Research Fields:

· Cellular Processes > Cell growth and death > Ferroptosis.(View pathway)
· Metabolism > Amino acid metabolism > Cysteine and methionine metabolism.
· Metabolism > Metabolism of other amino acids > Glutathione metabolism.
· Metabolism > Global and overview maps > Metabolic pathways.

Reference Citations:

1). Zou Y et al. Oregano Essential Oil Induces SOD1 and GSH Expression through Nrf2 Activation and Alleviates Hydrogen Peroxide-Induced Oxidative Damage in IPEC-J2 Cells. Oxid Med Cell Longev 2016;2016:5987183 (PubMed: 28105249) [IF=5.076]

Application: WB    Species:pig;    Sample:IPEC-J2 Cells

Figure 3: Effects of oregano essential oil (OEO) on g-glutamylcysteine ligase (GCLM, GCLC), and Cu/Zn-superoxide dismutase (SOD1) expression in IPEC-J2 cells. (a–d) IPEC-J2 cells were incubated with OEO 10


2). Li L et al. Silymarin protects against acrylamide-induced neurotoxicity via Nrf2 signalling in PC12 cells. Food Chem Toxicol 2017 Apr;102:93-101 (PubMed: 28137608) [IF=4.679]

Application: WB    Species:rat;    Sample:PC12 cells


3). Luo DD et al. Tetrahydrocurcumin and octahydrocurcumin, the primary and final hydrogenated metabolites of curcumin, possess superior hepatic-protective effect against acetaminophen-induced liver injury: Role of CYP2E1 and Keap1-Nrf2 pathway. Food Chem Toxicol 2018 Nov 10 (PubMed: 30423402) [IF=4.679]

4). Shi Z et al. Hepatoprotective and Anti-Oxidative Effects of Total Flavonoids From Qu Zhi Qiao (Fruit of Citrus Paradisi cv.Changshanhuyou) on Nonalcoholic Steatohepatitis In Vivo and In Vitro Through Nrf2-ARE Signaling Pathway. Front Pharmacol 2020 Apr 22;11:483 (PubMed: 32390839) [IF=4.225]

5). Lin X;Yang F;Huang J;Jiang S;Tang Y;Li J; et al. Ameliorate effect of pyrroloquinoline quinone against cyclophosphamide-induced nephrotoxicity by activating the Nrf2 pathway and inhibiting the NLRP3 pathway. Life Sci 2020 Jun 3;256:117901. (PubMed: 32504759) [IF=3.647]

6). Zhang S;Zhao J;Ma W; et al. Circ-Sirt1 inhibits proliferation, induces apoptosis, and ameliorates inflammation in human rheumatoid arthritis fibroblast-like synoviocytes. Autoimmunity 2021 Aug 25;1-12. (PubMed: 34431434)

7). et al. Hepatoprotective effect of pyrroloquinoline quinone against alcoholic liver injury through activating Nrf2-mediated antioxidant and inhibiting TLR4-mediated inflammation responses.

8). et al. Gly-Pro-Ala peptide and FGSHF3 exert protective effects in DON-induced toxicity and intestinal damage via decreasing oxidative stress.

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Catalog Number :

DF7268-BP
(Blocking peptide available as DF7268-BP)

Price/Size :

$350/1mg.
Tips: For modified antibodies, we provide modified peptides(0.5mg) and non-modified peptides(0.5mg).

Function :

Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. This mechanism is useful when non-specific binding is an issue, for example, in Western blotting (immunoblot) and immunohistochemistry (IHC). By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific; Specific binding will be absent from the western blot or immunostaining performed with the neutralized antibody.

Format and storage :

Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 10 mg/ml.The purity is >90%,tested by HPLC and MS.Storage Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C.

Precautions :

This product is for research use only. Not for use in diagnostic or therapeutic procedures.

Pig
100%
Bovine
100%
Rabbit
100%
Xenopus
78%
Horse
0%
Sheep
0%
Dog
0%
Zebrafish
0%
Chicken
0%
High similarity Medium similarity Low similarity No similarity
P48507 as Substrate
Site PTM Type Enzyme
K9 Sumoylation
K9 Ubiquitination
T17 Phosphorylation
K34 Ubiquitination
C35 S-Nitrosylation
K49 Ubiquitination
C72 S-Nitrosylation
K80 Ubiquitination
K90 Ubiquitination
K158 Sumoylation
K158 Ubiquitination
T164 Phosphorylation
S165 Phosphorylation
K169 Ubiquitination
K205 Sumoylation
K263 Acetylation
K263 Ubiquitination
Y265 Phosphorylation
K270 Ubiquitination
IMPORTANT: For western blots, incubate membrane with diluted antibody in 5% w/v milk , 1X TBS, 0.1% Tween®20 at 4°C with gentle shaking, overnight.

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