Tubulin gamma Antibody | Affinity Biosciences

WB
IF/ICC
Cites

1/5

Western blot analysis of Tubulin gamma expression in Rat brain lysate.

Western blot analysis of extracts from MCF7, using Tubulin gamma Antibody.

AF7012 staining MCF-7 cells by ICC/IF.

AF7012 staining Hela cells by IF/ICC.

Figure 4 Effects of FV on liver lipogenesis-related markers in the mice fed with HFD.

Product:	Tubulin gamma Antibody
Catalog:	AF7012
Description:	Rabbit polyclonal antibody to Tubulin gamma
Application:	WB IHC IF/ICC
Reactivity:	Human, Mouse, Rat
Prediction:	Zebrafish, Rabbit, Xenopus
Mol.Wt.:	51kDa; 51kD(Calculated).
Uniprot:	P23258
RRID:	AB_2839419

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

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100ul	$250	In stock
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1ml	$1200	In stock

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MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:000-1:5000, IHC 1:50-1:200, IF/ICC 1:200-500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse,Rat

Prediction:

Zebrafish(87%), Rabbit(87%), Xenopus(80%)

Clonality:

Polyclonal

Specificity:

Tubulin gamma Antibody detects endogenous levels of total Tubulin gamma.

RRID:

AB_2839419
Cite Format: Affinity Biosciences Cat# AF7012, RRID:AB_2839419.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

TUBG1, Gamma-1-tubulin, GCP-1, Gamma Tubulin, TUBGCP1, Tubulin gamma chain, TUBG, Tubulin, gamma polypeptide, Tubulin gamma-1 chain, Tubulin, gamma 1

Immunogens

Immunogen:

A synthesized peptide derived from human Tubulin gamma.

Uniprot:

P23258(TBG1_HUMAN) >>Visit HPA database.

Gene(ID):

TUBG1(7283)

Description:

TUBG1 Tubulin is the major constituent of microtubules. Gamma tubulin is found at microtubule organizing centers (MTOC) such as the spindle poles or the centrosome. Pericentriolar matrix component that regulates alpha/beta tubulin minus-end nucleation, centrosome duplication and spindle formation. Interacts with GCP2 and GCP3. Interacts with B9D2. Interacts with CDK5RAP2;

Sequence:

P23258 TBG1_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MPREIITLQLGQCGNQIGFEFWKQLCAEHGISPEGIVEEFATEGTDRKDVFFYQADDEHYIPRAVLLDLEPRVIHSILNSPYAKLYNPENIYLSEHGGGAGNNWASGFSQGEKIHEDIFDIIDREADGSDSLEGFVLCHSIAGGTGSGLGSYLLERLNDRYPKKLVQTYSVFPNQDEMSDVVVQPYNSLLTLKRLTQNADCVVVLDNTALNRIATDRLHIQNPSFSQINQLVSTIMSASTTTLRYPGYMNNDLIGLIASLIPTPRLHFLMTGYTPLTTDQSVASVRKTTVLDVMRRLLQPKNVMVSTGRDRQTNHCYIAILNIIQGEVDPTQVHKSLQRIRERKLANFIPWGPASIQVALSRKSPYLPSAHRVSGLMMANHTSISSLFERTCRQYDKLRKREAFLEQFRKEDMFKDNFDEMDTSREIVQQLIDEYHAATRPDYISWGTQEQ

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Rabbit

Zebrafish

Xenopus

Pig

Horse

Bovine

Sheep

Dog

Chicken

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P23258 As Substrate

P23258

Site	PTM Type	Enzyme	Source
S32	Phosphorylation		Uniprot
K48	Ubiquitination		Uniprot
S80	Phosphorylation	P24941 (CDK2)	Uniprot
K84	Ubiquitination		Uniprot
K113	Ubiquitination		Uniprot
S129	Phosphorylation		Uniprot
S131	Phosphorylation	Q8TDC3-2 (BRSK1)	Uniprot
K164	Ubiquitination		Uniprot
K193	Ubiquitination		Uniprot
S284	Phosphorylation		Uniprot
K287	Ubiquitination		Uniprot
R295	Methylation		Uniprot
K301	Ubiquitination		Uniprot
S306	Phosphorylation		Uniprot
T307	Phosphorylation		Uniprot
K335	Ubiquitination		Uniprot
S336	Phosphorylation		Uniprot
K344	Ubiquitination		Uniprot
K363	Ubiquitination		Uniprot
S364	Phosphorylation		Uniprot
S385	Phosphorylation	Q8TDC3 (BRSK1)	Uniprot
K397	Acetylation		Uniprot
K400	Acetylation		Uniprot
K400	Ubiquitination		Uniprot
K410	Ubiquitination		Uniprot
K415	Ubiquitination		Uniprot
T423	Phosphorylation		Uniprot
S424	Phosphorylation		Uniprot

Research Backgrounds

P23258_TBG1_HUMAN

Function:

Tubulin is the major constituent of microtubules. The gamma chain is found at microtubule organizing centers (MTOC) such as the spindle poles or the centrosome. Pericentriolar matrix component that regulates alpha/beta chain minus-end nucleation, centrosome duplication and spindle formation.

PTMs:

Phosphorylation at Ser-131 by BRSK1 regulates centrosome duplication, possibly by mediating relocation of gamma-tubulin and its associated proteins from the cytoplasm to the centrosome.

Subcellular Location:

Cytoplasm>Cytoskeleton>Microtubule organizing center>Centrosome.

Subunit Structure:

Interacts with TUBGCP2 and TUBGCP3. Interacts with B9D2 (By similarity). Interacts with CDK5RAP2; the interaction is leading to centrosomal localization of TUBG1 and CDK5RAP2. Interacts with PIFO. Interacts with SAS6 and NUP62 at the centrosome.

Family&Domains:

Belongs to the tubulin family.

Research Fields

· Human Diseases > Infectious diseases: Viral > Human papillomavirus infection.

References

1). Ficus hirta Vahl. Ameliorates Nonalcoholic Fatty Liver Disease through Regulating Lipid Metabolism and Gut Microbiota. Oxidative Medicine and Cellular Longevity (PubMed: 35592528)

Application: WB Species: Mouse Sample:

Figure 4 Effects of FV on liver lipogenesis-related markers in the mice fed with HFD. (a–h) The relative mRNA expression levels of Acaca, Cd36, Cpt1α, Srebp-1, Hmgcr, Pparα, Pparγ, and Scd1 were determined by qRT-PCR. (i) The lipid metabolism relevant protein levels of SREBP-1, ACOX1, CD36, CPT1α, and HMGCR were analyzed by western blotting, and the relative ratios were calculated. (j–l) Serum IL-6, IL-1β, and TNF-α were identified by ELISA kits. (m, n) The relative mRNA expression levels of IL-1β, TNF-α, and Ccl5 were identified by qRT-PCR. (p) The proinflammatory factor protein levels of IL-1β, IL-6, and TNF-α were analyzed by western blotting, and their relative ratios were calculated. The densitometry was obtained by averaging repeated experiments results, normalized to GAPDH. The data are expressed as the mean ± SD, n = 3. #P < 0.05 means there is significant difference between the NFD group and the HFD group. ∗P < 0.05 means the difference between the HFD group and the FV-L group or the FV-H group is significant.

Restrictive clause

Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

For Research Use Only.
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Tubulin gamma Antibody - #AF7012