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  • Product Name
    beta Actin Antibody
  • Catalog No.
    AF7018
  • RRID
    AB_2839420
  • Source
    Rabbit
  • Application
    WB,IHC,IF/ICC
  • Reactivity
    Human, Mouse, Rat, Pig, Zebrafish, Bovine, Rabbit, Dog, Monkey, Fish
  • Prediction
    Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus
  • UniProt
  • Mol.Wt
    43kD;
    42kDa(Calculated).
  • Concentration
    1mg/ml
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Product Information

Alternative Names:Expand▼

ACTB; Actin; cytoplasmic 1; Beta-actin; Beta actin; BRWS1; β actin;b actin; Actin beta; Beta cytoskeletal actin; PS1TP5-binding protein 1; PS1TP5BP1;

Applications:

WB 1:3000-1:20000, IHC 1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.

Reactivity:

Human, Mouse, Rat, Pig, Zebrafish, Bovine, Rabbit, Dog, Monkey, Fish

Predicted Reactivity:

Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus

Source:

Rabbit

Clonality:

Polyclonal

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Specificity:

Beta actin antibody detects endogenous levels of total Beta actin.

RRID:

AB_2839420
Please cite this product as: Affinity Biosciences Cat# AF7018, RRID:AB_2839420.

Format:

Liquid

Concentration:

1mg/ml

Storage Condition and Buffer:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt.

Immunogen Information in 3D

Immunogen:

A synthesized peptide derived from human Beta actin.

Uniprot:



>>Visit The Human Protein Atlas

Gene ID:

Gene Name:

ACTB

Molecular Weight:

Observed Mol.Wt.: 43kD.
Predicted Mol.Wt.: 42kDa(Calculated)..

Subcellular Location:

Cytoplasm > cytoskeleton. Localized in cytoplasmic mRNP granules containing untranslated mRNAs.

Description:

Actin, a ubiquitous eukaryotic protein, is the major component of the cytoskeleton. At least six isoforms are known in mammals. Nonmuscle β- and γ-actin, also known as cytoplasmic actin, are predominantly expressed in nonmuscle cells, controlling cell structure and motility.

Sequence:
MDDDIAALVVDNGSGMCKAGFAGDDAPRAVFPSIVGRPRHQGVMVGMGQKDSYVGDEAQSKRGILTLKYPIEHGIVTNWDDMEKIWHHTFYNELRVAPEEHPVLLTEAPLNPKANREKMTQIMFETFNTPAMYVAIQAVLSLYASGRTTGIVMDSGDGVTHTVPIYEGYALPHAILRLDLAGRDLTDYLMKILTERGYSFTTTAEREIVRDIKEKLCYVALDFEQEMATAASSSSLEKSYELPDGQVITIGNERFRCPEALFQPSFLGMESCGIHETTFNSIMKCDVDIRKDLYANTVLSGGTTMYPGIADRMQKEITALAPSTMKIKIIAPPERKYSVWIGGSILASLSTFQQMWISKQEYDESGPSIVHRKCF

Research Background

Function:

Actin is a highly conserved protein that polymerizes to produce filaments that form cross-linked networks in the cytoplasm of cells. Actin exists in both monomeric (G-actin) and polymeric (F-actin) forms, both forms playing key functions, such as cell motility and contraction. In addition to their role in the cytoplasmic cytoskeleton, G- and F-actin also localize in the nucleus, and regulate gene transcription and motility and repair of damaged DNA.

Post-translational Modifications:

ISGylated.

Oxidation of Met-44 and Met-47 by MICALs (MICAL1, MICAL2 or MICAL3) to form methionine sulfoxide promotes actin filament depolymerization. MICAL1 and MICAL2 produce the (R)-S-oxide form. The (R)-S-oxide form is reverted by MSRB1 and MSRB2, which promote actin repolymerization.

Monomethylation at Lys-84 (K84me1) regulates actin-myosin interaction and actomyosin-dependent processes. Demethylation by ALKBH4 is required for maintaining actomyosin dynamics supporting normal cleavage furrow ingression during cytokinesis and cell migration.

Methylated at His-73 by SETD3. Methylation at His-73 is required for smooth muscle contraction of the laboring uterus during delivery (By similarity).

N-terminal acetylation by NAA80 affects actin filament depolymerization and elongation, including elongation driven by formins. In contrast, filament nucleation by the Arp2/3 complex is not affected.

(Microbial infection) Monomeric actin is cross-linked by V.cholerae toxins RtxA and VgrG1 in case of infection: bacterial toxins mediate the cross-link between Lys-50 of one monomer and Glu-270 of another actin monomer, resulting in formation of highly toxic actin oligomers that cause cell rounding. The toxin can be highly efficient at very low concentrations by acting on formin homology family proteins: toxic actin oligomers bind with high affinity to formins and adversely affect both nucleation and elongation abilities of formins, causing their potent inhibition in both profilin-dependent and independent manners.

Subcellular Location:

Cytoplasm>Cytoskeleton. Nucleus.
Note: Localized in cytoplasmic mRNP granules containing untranslated mRNAs.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte

Subunit Structure:

Polymerization of globular actin (G-actin) leads to a structural filament (F-actin) in the form of a two-stranded helix. Each actin can bind to 4 others. Identified in a IGF2BP1-dependent mRNP granule complex containing untranslated mRNAs. Component of the BAF complex, which includes at least actin (ACTB), ARID1A, ARID1B/BAF250, SMARCA2, SMARCA4/BRG1, ACTL6A/BAF53, ACTL6B/BAF53B, SMARCE1/BAF57 SMARCC1/BAF155, SMARCC2/BAF170, SMARCB1/SNF5/INI1, and one or more of SMARCD1/BAF60A, SMARCD2/BAF60B, or SMARCD3/BAF60C. In muscle cells, the BAF complex also contains DPF3. Found in a complex with XPO6, Ran, ACTB and PFN1. Interacts with XPO6 and EMD. Interacts with ERBB2. Interacts with GCSAM. Interacts with TBC1D21 (By similarity). Interacts with CPNE1 (via VWFA domain) and CPNE4 (via VWFA domain) (By similarity). Interacts with DHX9 (via C-terminus); this interaction is direct and mediates the attachment to nuclear ribonucleoprotein complexes. Interacts with FAM107A.

Similarity:

Belongs to the actin family.

Research Fields

Research Fields:

· Cellular Processes > Transport and catabolism > Phagosome.(View pathway)
· Cellular Processes > Cell growth and death > Apoptosis.(View pathway)
· Cellular Processes > Cellular community - eukaryotes > Focal adhesion.(View pathway)
· Cellular Processes > Cellular community - eukaryotes > Adherens junction.(View pathway)
· Cellular Processes > Cellular community - eukaryotes > Tight junction.(View pathway)
· Cellular Processes > Cell motility > Regulation of actin cytoskeleton.(View pathway)
· Environmental Information Processing > Signal transduction > Rap1 signaling pathway.(View pathway)
· Environmental Information Processing > Signal transduction > Hippo signaling pathway.(View pathway)
· Human Diseases > Infectious diseases: Bacterial > Bacterial invasion of epithelial cells.
· Human Diseases > Infectious diseases: Bacterial > Vibrio cholerae infection.
· Human Diseases > Infectious diseases: Bacterial > Pathogenic Escherichia coli infection.
· Human Diseases > Infectious diseases: Bacterial > Shigellosis.
· Human Diseases > Infectious diseases: Bacterial > Salmonella infection.
· Human Diseases > Infectious diseases: Viral > Influenza A.
· Human Diseases > Cancers: Overview > Proteoglycans in cancer.
· Human Diseases > Cancers: Specific types > Hepatocellular carcinoma.(View pathway)
· Human Diseases > Cardiovascular diseases > Hypertrophic cardiomyopathy (HCM).
· Human Diseases > Cardiovascular diseases > Arrhythmogenic right ventricular cardiomyopathy (ARVC).
· Human Diseases > Cardiovascular diseases > Dilated cardiomyopathy (DCM).
· Human Diseases > Cardiovascular diseases > Viral myocarditis.
· Organismal Systems > Immune system > Platelet activation.(View pathway)
· Organismal Systems > Immune system > Leukocyte transendothelial migration.(View pathway)
· Organismal Systems > Endocrine system > Thyroid hormone signaling pathway.(View pathway)
· Organismal Systems > Endocrine system > Oxytocin signaling pathway.
· Organismal Systems > Digestive system > Gastric acid secretion.

Reference Citations:

1). Tang H et al. circKIF4A acts as a prognostic factor and mediator to regulate the progression of triple-negative breast cancer. Mol Cancer 2019 Feb 11;18(1):23 (PubMed: 30744636) [IF=15.302]

2). Li X;Wang J;Gong X;Zhang M;Kang S;Shu B;Wei Z;Huang ZS;Li D; et al. Upregulation of BCL-2 by acridone derivative through gene promoter i-motif for alleviating liver damage of NAFLD/NASH. Nucleic Acids Res 2020 Sep 4;48(15):8255-8268. (PubMed: 32710621) [IF=11.501]

Application: WB    Species:mouse;    Sample:liver

Figure 7. Effect of A22 on ameliorating apoptosis, ER stress, inflammation, metabolic syndrome, and fibrogenesis in HF diet-fed mice. (A) Effect of A22 on BCL-2 gene transcription. (B) Effect of A22 on BAX gene transcription. (C) Effect of A22 on expressions of apoptosis-related proteins in liver. The extracted proteins from the liver were immunoblotted with specific antibodies, and quantified based on the loading control of ACTIN. (D) Effect of A22 on ER stress. The UPR proteins (IRE-1, PERK, elF-2 and CHOP) were analyzed by using western Blot. (E) Effect of A22 on expressions of inflammatory factors. (F) Effect of A22 on expressions of fibrogenic proteins.


3). Zhou P et al. Multifunctional nanoparticles based on a polymeric copper chelator for combination treatment of metastatic breast cancer. Biomaterials 2019 Jan 4;195:86-99 (PubMed: 30623789) [IF=10.317]

4). Hou B;Wang F;Liu T;Wang Z; et al. Reproductive toxicity of polystyrene microplastics: In vivo experimental study on testicular toxicity in mice. J Hazard Mater 2020 Oct 5;124028. (PubMed: 33087287) [IF=9.038]

5). Zhang D et al. Cold to Hot: Rational Design of Minimalist Multifunctional Photo-Immunotherapy Nanoplatform toward Boosting Immunotherapy Capability. ACS Appl Mater Interfaces 2019 Aug 20 (PubMed: 31429272) [IF=8.758]

6). Zhong W;Hou H;Liu T;Su S;Xi X;Liao Y;Xie R;Jin G;Liu X;Zhu L;Zhang H;Song X;Yang C;Sun T;Cao H;Wang B; et al. Cartilage Oligomeric Matrix Protein promotes epithelial-mesenchymal transition by interacting with Transgelin in Colorectal Cancer. Theranostics 2020 Jul 9;10(19):8790-8806. (PubMed: 32754278) [IF=8.579]

7). Hu M;Wu Y;Yang C;Wang X;Wang W;Zhou L;Zeng T;Zhou J;Wang C;Lao G;Yan L;Ren M; et al. Novel Long Non-Coding RNA lnc-URIDS Delays Diabetic Wound Healing By Targeting Plod1. Diabetes 2020 Aug 14;db200147. (PubMed: 32801140) [IF=7.720]

8). He R et al. circGFRA1 and GFRA1 act as ceRNAs in triple negative breast cancer by regulating miR-34a. J Exp Clin Cancer Res 2017 Oct 16;36(1):145 (PubMed: 29037220) [IF=7.068]

9). Liu P et al. Melatonin Regulates Breast Cancer Progression by the lnc010561/miR-30/FKBP3 Axis. Mol Ther Nucleic Acids 2019 Dec 24;19:765-774 (PubMed: 31955008) [IF=7.032]

10). Wang J;Yu P;Xie X;Wu L;Zhou M;Huan F;Jiang L;Gao R; et al. Bisphenol F induces nonalcoholic fatty liver disease-like changes: Involvement of lysosome disorder in lipid droplet deposition. Environ Pollut 2021 Feb 15;271:116304. (PubMed: 33401208) [IF=6.792]

11). Ding S et al. Sensitive and Selective Measurement of Hydroxyl Radicals at Subcellular Level with Tungsten Nanoelectrodes. Anal Chem 2020 Jan 13 (PubMed: 31927939) [IF=6.785]

12). Fan H et al. Bacteroides fragilis Strain ZY-312 Defense against Cronobacter sakazakii-Induced Necrotizing Enterocolitis In Vitro and in a Neonatal Rat Model. mSystems 2019 Aug 6;4(4) (PubMed: 31387931) [IF=6.633]

13). Duan X et al. Inhibition of keratinocyte necroptosis mediated by RIPK1/RIPK3/MLKL provides a protective effect against psoriatic inflammation. Cell Death Dis 2020 Feb 19;11(2):134 (PubMed: 32075957) [IF=6.304]

14). Chen Z;Wu C;Liu Y;Li H;Zhu Y;Huang C;Lin H;Qiao Q;Huang M;Zhu Q;Wang L; et al. ELABELA attenuates deoxycorticosterone acetate/salt-induced hypertension and renal injury by inhibition of NADPH oxidase/ROS/NLRP3 inflammasome pathway. Cell Death Dis 2020 Aug 22;11(8):698. (PubMed: 32829380) [IF=6.304]

15). He XF;Xu JH;Li G;Li MY;Li LL;Pei Z;Zhang LY;Hu XQ; et al. NLRP3-dependent microglial training impaired the clearance of amyloid-beta and aggravated the cognitive decline in Alzheimer’s disease. Cell Death Dis 2020 Oct 13;11(10):849. (PubMed: 33051464) [IF=6.304]

16). Cai Y;Leng S;Ma Y;Xu T;Chang D;Ju S; et al. Dynamic change of MMP-9 in diabetic stroke visualized by optical imaging and treated with CD28 superagonist. Biomater Sci 2021 Apr 7;9(7):2562-2570. (PubMed: 33585848) [IF=6.183]

17). Yi S et al. Tcf12, A Member of Basic Helix-Loop-Helix Transcription Factors, Mediates Bone Marrow Mesenchymal Stem Cell Osteogenic Differentiation In Vitro and In Vivo. Stem Cells 2017 Feb;35(2):386-397 (PubMed: 27574032) [IF=6.022]

Application: WB    Species:mouse;    Sample:Not available

Figure 5. Down regulation of Tcf12 accelerate and potentiate bone repair in vivo. (A‐B) Quantification of Alp activity at day 14 in U‐0126 and LDN‐193189 treated shTcf12 and scrambled groups.


18). Li C et al. Oxyberberine, a novel gut microbiota-mediated metabolite of berberine, possesses superior anti-colitis effect: impact on intestinal epithelial barrier, gut microbiota profile and TLR4-MyD88-NF-κB pathway. Pharmacol Res 2019 Dec 18:104603 (PubMed: 31863867) [IF=5.893]

19). Zhang P;Tao W;Lu C;Fan L;Jiang Q;Yang C;Shang E;Cheng H;Che C;Duan J;Zhao M; et al. Bruceine A induces cell growth inhibition and apoptosis through PFKFB4/GSK3β signaling in pancreatic cancer. Pharmacol Res 2021 May 13;105658. (PubMed: 33992797) [IF=5.893]

20). Zhou H et al. miR-506 enhances the sensitivity of human colorectal cancer cells to oxaliplatin by suppressing MDR1/P-gp expression. Cell Prolif 2017 Jun;50(3) (PubMed: 28217977) [IF=5.753]

Application: WB    Species:human;    Sample:Not available

FIGURE 3  miR-506 down-regulated MDR1/P-gp expression in HCT116-OxR. A, The mRNA level of MDR1 was decreased after transfection with the miR-506 mimic of the relative chemoresistant genes as demonstrated by qRT-PCR. B, The protein level of MDR1 was decreased after transfection with the miR-506 mimic of the relative chemoresistant proteins as demonstrated by Western blot. C, Expression of P-gp detected by immunofluorescence staining.HCT116-OxR-miR-506 cells showed low levels of fluorescent staining of P-gp, whereas maximal staining of P-gp was observed in HCT116-OxR cells, readily distinguished from background. Zoom: 200×. *P<.05)


21). Ma S;Zhu L;Fan X;Luo T;Liu D;Liang Z;Hu X;Shi T;Tan W;Wang Z; et al. Melatonin derivatives combat with inflammation-related cancer by targeting the Main Culprit STAT3. Eur J Med Chem 2020 Nov 17;113027. (PubMed: 33248852) [IF=5.572]

22). Fan X;Li J;Long L;Shi T;Liu D;Tan W;Zhang H;Wu X;Lei X;Wang Z; et al. Design, synthesis and biological evaluation of N-anthraniloyl tryptamine derivatives as pleiotropic molecules for the therapy of malignant glioma. Eur J Med Chem 2021 May 29;222:113564. (PubMed: 34091208) [IF=5.572]

23). He F et al. Irradiation-induced osteocyte damage promotes HMGB1-mediated osteoclastogenesis in vitro. J Cell Physiol 2019 Feb 20 (PubMed: 30786022) [IF=5.546]

24). Ding L et al. Noncoding transcribed ultraconserved region (T-UCR) UC.48+ is a novel regulator of high-fat diet induced myocardial ischemia/reperfusion injury. J Cell Physiol 2019 Jun;234(6):9849-9861 (PubMed: 30417395) [IF=5.546]

25). Zhao YR et al. HEG1 indicates poor prognosis and promotes hepatocellular carcinoma invasion, metastasis, and EMT by activating Wnt/β-catenin signaling. Clin Sci (Lond) 2019 Jul 5 (PubMed: 31278131) [IF=5.223]

26). Yan L;Gao S;Shui S;Liu S;Qu H;Liu C;Zheng L; et al. Small interfering RNA-loaded chitosan hydrochloride/carboxymethyl chitosan nanoparticles for ultrasound-triggered release to hamper colorectal cancer growth in vitro. Int J Biol Macromol 2020 Jun 27;S0141-8130(20)33694-1. (PubMed: 32603733) [IF=5.162]

27). Wu Z et al. In vitro and in vivo anti-Listeria effect of Succinoglycan Riclin through regulating MAPK/IL-6 axis and metabolic profiling. Int J Biol Macromol 2020 May 1;150:802-813 (PubMed: 32057883) [IF=5.162]

28). Wang L et al. Development of anisamide-targeted PEGylated gold nanorods to deliver epirubicin for chemo-photothermal therapy in tumor-bearing mice. Int J Nanomedicine 2019 Mar 8;14:1817-1833 (PubMed: 30880982) [IF=5.115]

29). Tao HC et al. CD47 Deficiency in Mice Exacerbates Chronic Fatty Diet-Induced Steatohepatitis Through Its Role in Regulating Hepatic Inflammation and Lipid Metabolism. Front Immunol 2020 Feb 25;11:148 (PubMed: 32158445) [IF=5.085]

30). Zhang J;Hu X;Dong X;Chen W;Zhang L;Chang Y;Wu Y;Wei W; et al. Regulation of T Cell Activities in Rheumatoid Arthritis by the Novel Fusion Protein IgD-Fc-Ig. Front Immunol 2020 May 15;11:755. (PubMed: 32499775) [IF=5.085]

Application: WB    Species:mouse;    Sample:spleen

FIGURE 9 | Effects of IgD-Fc-Ig (DG) on the protein expression of Lck, p-Lck, ZAP70 and p-ZAP70. (A–C) Western blot analysis of p-Lck and p-ZAP70 expression in PBMCs of RA patients, PBMCs were treated with IgD (3µg/mL) and IgD-Fc-Ig (1, 3, 10µg/mL) for 48h and lysed. (D–F) Western blot analysis of Lck, p-Lck, ZAP70 and p-ZAP70 expression in CIA mice. Mice spleens from each group were isolated and homogenized in lysis buffer.


31). Huang H et al. Nutritional Preconditioning of Apigenin Alleviates Myocardial Ischemia/Reperfusion Injury via the Mitochondrial Pathway Mediated by Notch1/Hes1. Oxid Med Cell Longev 2019 Mar 20;2019:7973098 (PubMed: 31015891) [IF=5.076]

32). Cai BB et al. Acid sphingomyelinase downregulation alleviates vascular endothelial leptin resistance in rats. Acta Pharmacol Sin 2019 Dec 17 (PubMed: 31848475) [IF=5.064]

33). Zhou WJ;Wang DD;Tao J;Tai Y;Zhou ZW;Wang Z;Guo PP;Sun WY;Chen JY;Wu HX;Yan SX;Zhang LL;Wang QT;Wei W; et al. Deficiency of β-arrestin2 exacerbates inflammatory arthritis by facilitating plasma cell formation. Acta Pharmacol Sin 2020 Aug 27. (PubMed: 32855529) [IF=5.064]

34). Liu N;Sun Q;Xu H;Yu X;Chen W;Wei H;Jiang J;Xu Y;Lu W; et al. Hyperuricemia induces lipid disturbances mediated by LPCAT3 upregulation in the liver. FASEB J 2020 Aug 11. (PubMed: 32780898) [IF=4.966]

35). Li C;Chen Q;Zhou Y;Niu Y;Wang X;Li X;Zheng H;Wei T;Zhao L;Gao H; et al. S100A2 promotes glycolysis and proliferation via GLUT1 regulation in colorectal cancer. FASEB J 2020 Aug 20. (PubMed: 32816365) [IF=4.966]

36). Du P;Hu T;An Z;Li P;Liu L; et al. In vitro and in vivo synergistic efficacy of ceritinib combined with programmed cell death ligand‐1 inhibitor in anaplastic lymphoma kinase‐rearranged non‐small‐cell lung cancer. Cancer Sci 2020 Jun;111(6):1887-1898. (PubMed: 32227409) [IF=4.966]

37). Jiang D;Mo Q;Sun X;Wang X;Dong M;Zhang G;Chen F;Zhao Q; et al. Pyruvate dehydrogenase kinase 4‐mediated metabolic reprogramming is involved in rituximab resistance in diffuse large B‐cell lymphoma by affecting the expression of MS4A1/CD20. Cancer Sci 2021 Sep;112(9):3585-3597. (PubMed: 34252986) [IF=4.966]

38). Lin H et al. Overcoming Taxol-resistance in A549 Cells: A Comprehensive Strategy of Targeting P-gp transporter, AKT/ERK Pathways, and Cytochrome P450 Enzyme CYP1B1 by 4-hydroxyemodin. Biochem Pharmacol 2019 Nov 26:113733 (PubMed: 31783010) [IF=4.960]

39). Hong H;Cheung YM;Cao X;Wu Y;Li C;Tian XY; et al. REV-ERBα agonist SR9009 suppresses IL-1β production in macrophages through BMAL1-dependent inhibition of inflammasome. Biochem Pharmacol 2021 Jul 26;192:114701. (PubMed: 34324866) [IF=4.960]

40). Yang Z et al. CCL2/CCR2 Axis Promotes the Progression of Salivary Adenoid Cystic Carcinoma via Recruiting and Reprogramming the Tumor-Associated Macrophages. Front Oncol 2019 Apr 9;9:231 (PubMed: 31024838) [IF=4.848]

41). Ding J;Wang Q;Guo N;Wang H;Chen H;Ni G;Li P; et al. CircRNA circ_0072995 promotes the progression of epithelial ovarian cancer by modulating miR-147a/CDK6 axis. Aging (Albany NY) 2020 Sep 2;12. (PubMed: 32877369) [IF=4.831]

42). Li Y;Jiang B;He Z;Zhu H;He R;Fan S;Wu X;Xie L;He X; et al. circIQCH sponges miR-145 to promote breast cancer progression by upregulating DNMT3A expression. Aging (Albany NY) 2020 Aug 3;12(15):15532-15545. (PubMed: 32756009) [IF=4.831]

43). Shen Y;Sun Z;Mao S;Zhang Y;Jiang W;Wang H; et al. IRF-1 contributes to the pathological phenotype of VSMCs during atherogenesis by increasing CCL19 transcription. Aging (Albany NY) 2020 Nov 16;13(1):933-943. (PubMed: 33424012) [IF=4.831]

44). Gong Q;Jiang Y;Pan X;You Y; et al. Fractalkine aggravates LPS‐induced macrophage activation and acute kidney injury via Wnt/β‐catenin signalling pathway. J Cell Mol Med 2021 Jun 7. (PubMed: 34101346) [IF=4.658]

45). Luan X et al. Anisamide-targeted PEGylated gold nanoparticles designed to target prostate cancer mediate: Enhanced systemic exposure of siRNA, tumour growth suppression and a synergistic therapeutic response in combination with paclitaxel in mice. Eur J Pharm Biopharm 2019 Feb 16 (PubMed: 30779980) [IF=4.604]

46). Wang X et al. Autophagy enhanced antitumor effect in K562 and K562/ADM cells using realgar transforming solution. Biomed Pharmacother 2018 Feb;98:252-264 (PubMed: 29272786) [IF=4.545]

47). Zhang L et al. Emodin targets mitochondrial cyclophilin D to induce apoptosis in HepG2 cells. Biomed Pharmacother 2017 Jun;90:222-228 (PubMed: 28363167) [IF=4.545]

Application: WB    Species:human;    Sample:Not available

Fig. 2. Effects of CsA on emodin-induced apoptosis. The cells were treated with emodin for 48 h in the presence or absence of CsA (5mM), then assays were performed. (A) Analysis of apoptosis by nuclear condensation. The Hoechst 33342 staining showed typical apoptotic morphology changes after emodin treatment. The images were acquired by inverted fluorescence microscopy. (B) Analysis of apoptosis by Annexin V/PI double-staining assay. (C) Determination of Cyto-C level in mitochondria and cytosol by western blots. b-actin and VDAC1 were used as internal control.


48). Huang YF et al. Therapeutic effect of Brucea javanica oil emulsion on experimental Crohn's disease in rats: Involvement of TLR4/ NF-κB signaling pathway. Biomed Pharmacother 2019 Mar 19;114:108766 (PubMed: 30901719) [IF=4.545]

49). Duan S et al. Effect of vitexin on alleviating liver inflammation in a dextran sulfate sodium (DSS)-induced colitis model. Biomed Pharmacother 2020 Jan;121:109683 (PubMed: 31810123) [IF=4.545]

50). Zhou Q et al. PolyG mitigates silica-induced pulmonary fibrosis by inhibiting nucleolin and regulating DNA damage repair pathway. Biomed Pharmacother 2020 Feb 6;125:109953 (PubMed: 32036217) [IF=4.545]

51). Zhong L;Zhang H;Ding ZF;Li J;Lv JW;Pan ZJ;Xu DX;Yin ZS; et al. Erythropoietin-Induced Autophagy Protects Against Spinal Cord Injury and Improves Neurological Function via the Extracellular-Regulated Protein Kinase Signaling Pathway. Mol Neurobiol 2020 Jul 9. (PubMed: 32647973) [IF=4.500]

52). Xie J et al. circEPSTI1 regulates ovarian cancer progression via decoying miR-942. J Cell Mol Med 2019 Mar 19 (PubMed: 30887698) [IF=4.486]

53). Peng H et al. circCPA4 acts as a prognostic factor and regulates the proliferation and metastasis of glioma. J Cell Mol Med 2019 Aug 19 (PubMed: 31424161) [IF=4.486]

54). Wang S et al. Effect of (R)-salbutamol on the switch of phenotype and metabolic pattern in LPS-induced macrophage cells. J Cell Mol Med 2020 Jan;24(1):722-736 (PubMed: 31680470) [IF=4.486]

55). Gong Q;Jiang Y;Pan X;You Y; et al. Fractalkine aggravates LPS‐induced macrophage activation and acute kidney injury via Wnt/β‐catenin signalling pathway. J Cell Mol Med 2021 Jun 7. (PubMed: 34101346) [IF=4.486]

56). Cai Y et al. Diffusion Tensor Imaging Evaluation of Axonal/White Matter Remodeling in a Mouse Model of Diabetic Stroke Treated with Novel p38 MAPK Inhibitor, VCP979. J Biomed Nanotechnol 2018 Mar 1;14(3):585-593 (PubMed: 29663930) [IF=4.483]

57). Lv C et al. Long-Term DL-3-n-Butylphthalide Treatment Alleviates Cognitive Impairment Correlate With Improving Synaptic Plasticity in SAMP8 Mice. Front Aging Neurosci 2018 Jul 5;10:200 (PubMed: 30026693) [IF=4.362]

58). Yang B;Yu Q;Chang B;Guo Q;Xu S;Yi X;Cao S; et al. MOTS-c interacts synergistically with exercise intervention to regulate PGC-1α expression, attenuate insulin resistance and enhance glucose metabolism in mice via AMPK signaling pathway. Biochim Biophys Acta Mol Basis Dis 2021 Mar 12;166126. (PubMed: 33722744) [IF=4.352]

59). Li CL et al. Comparison of anti-inflammatory effects of berberine, and its natural oxidative and reduced derivatives from Rhizoma Coptidis in vitro and in vivo. Phytomedicine 2019 Jan;52:272-283 (PubMed: 30599908) [IF=4.268]

60). Ma J et al. Long noncoding RNA lnc-ABCA12-3 promotes cell migration, invasion, and proliferation by regulating fibronectin 1 in esophageal squamous cell carcinoma. J Cell Biochem 2019 Sep 12 (PubMed: 31512786) [IF=4.237]

61). Li C;Bai X;Liu X;Zhang Y;Liu L;Zhang L;Xu F;Yang Y;Liu M; et al. Disruption of Epithelial Barrier of Caco-2 Cell Monolayers by excretory secretory products of Trichinella Spiralis might be Related to serine Protease. Front Microbiol 2021 Mar 17;12:634185. (PubMed: 33815318) [IF=4.235]

62). Gong J et al. Berberine Attenuates Intestinal Mucosal Barrier Dysfunction in Type 2 Diabetic Rats. Front Pharmacol 2017 Feb 3;8:42 (PubMed: 28217099) [IF=4.225]

63). Wu X et al. Protective Effect of Patchouli Alcohol Against High-Fat Diet Induced Hepatic Steatosis by Alleviating Endoplasmic Reticulum Stress and Regulating VLDL Metabolism in Rats. Front Pharmacol 2019 Oct 1;10:1134 (PubMed: 31632274) [IF=4.225]

64). Zhu H et al. Synthesis of Chalcone Derivatives: Inducing Apoptosis of HepG2 Cells via Regulating Reactive Oxygen Species and Mitochondrial Pathway. Front Pharmacol 2019 Nov 15;10:1341 (PubMed: 31803052) [IF=4.225]

65). You Y;He Q;Lu H;Zhou X;Chen L;Liu H;Lu Z;Liu D;Liu Y;Zuo D;Fu X;Kwan H;Zhao X; et al. Silibinin Induces G2/M Cell Cycle Arrest by Activating Drp1-Dependent Mitochondrial Fission in Cervical Cancer. Front Pharmacol 2020 Mar 12;11:271. (PubMed: 32226384) [IF=4.225]

66). Liu X et al. Silencing c-Myc Enhances the Antitumor Activity of Bufalin by Suppressing the HIF-1α/SDF-1/CXCR4 Pathway in Pancreatic Cancer Cells. Front Pharmacol 2020 Apr 17;11:495 (PubMed: 32362830) [IF=4.225]

67). Shi YR;Wu Z;Xiong K;Liao QJ;Ye X;Yang P;Zu XB; et al. Circular RNA circKIF4A sponges miR-375/1231 to promote bladder cancer progression by upregulating NOTCH2 expression. Front Pharmacol 2020 May 8;11:605. (PubMed: 32457613) [IF=4.225]

68). Zheng JN;Zhuo JY;Nie J;Liu YL;Chen BY;Wu AZ;Li YC; et al. Phenylethanoid Glycosides From Callicarpa kwangtungensis Chun Attenuate TNF-α-Induced Cell Damage by Inhibiting NF-κB Pathway and Enhancing Nrf2 Pathway in A549 Cells. Front Pharmacol 2021 Jul 7;12:693983. (PubMed: 34305604) [IF=4.225]

69). Jia Y et al. Overexpression of CD59 inhibits apoptosis of T-acute lymphoblastic leukemia via AKT/Notch1 signaling pathway. Cancer Cell Int 2019 Jan 8;19:9 (PubMed: 30636930) [IF=4.175]

70). Wang W;Wang M;Xu J;Long F;Zhan X; et al. Overexpressed GATA3 enhances the sensitivity of colorectal cancer cells to oxaliplatin through regulating MiR-29b. Cancer Cell Int 2020 Jul 24;20:339. (PubMed: 32760217) [IF=4.175]

71). Zhu M;Wang Y;Wang F;Li L;Qiu X; et al. CircFBXL5 promotes the 5-FU resistance of breast cancer via modulating miR-216b/HMGA2 axis. Cancer Cell Int 2021 Jul 19;21(1):384. (PubMed: 34281530) [IF=4.175]

72). Dai Z et al. Carnosine ameliorates age-related dementia via improving mitochondrial dysfunction in SAMP8 mice. Food Funct 2020 Mar 5 (PubMed: 32134423) [IF=4.171]

73). Fu C;Zheng Y;Lin K;Wang H;Chen T;Li L;Huang J;Lin W;Zhu J;Li P;Fu X;Lin Z; et al. Neuroprotective Effect of Apigenin Against Hypoxic-Ischemic Brain Injury in Neonatal Rats via the PI3K/Akt/Nrf2 signaling pathway. Food Funct 2021 Feb 18. (PubMed: 33599218) [IF=4.171]

74). Hu F;Chen X;Gao J;Shen Y;Yang J; et al. CircDIP2C ameliorates oxidized low-density lipoprotein-induced cell dysfunction by binding to miR-556-5p to induce TET2 in human umbilical vein endothelial cells. Vascul Pharmacol 2021 Jun 18;106887. (PubMed: 34147657) [IF=4.152]

75). Jiang D;He Y;Mo Q;Liu E;Li X;Huang L;Zhang Q;Chen F;Li Y;Shao H; et al. PRICKLE1, a Wnt/PCP signaling component, is overexpressed and associated with inferior prognosis in acute myeloid leukemia. J Transl Med 2021 May 17;19(1):211. (PubMed: 34001134) [IF=4.124]

76). Kong Y et al. CircPLK1 sponges miR-296-5p to facilitate triple-negative breast cancer progression. Epigenomics 2019 Jul 24 (PubMed: 31337246) [IF=4.112]

77). Li Z et al. The role of PGC-1α and MRP1 in lead-induced mitochondrial toxicity in testicular Sertoli cells. Toxicology 2016 Apr 29;355-356:39-48 (PubMed: 27236077) [IF=4.099]

Application: WB    Species:mouse;    Sample:Not available

Fig. 6. Role of MRP1 on lead-induced mitochondrial toxicity. (A) Expression of MRP1 in the mitochondria of Sertoli cells with various concentrations of lead acetate. (B) Expression of MRP1 in the mitochondria of Sertoli cells with continuous time exposure to lead acetate (20mM). (C) Expression of MRP1 in the mitochondria of the TM4 cells and MRP1()TM4 cells. (D) Transport activity of MRP1 in the mitochondria of the TM4 cells and MRP1()TM4 cells. (E) The accumulation of lead in the mitochondria of TM4 cells and MRP1()TM4 cells. Data represent mean  SD of at least three independent experiments (*P < 0.05, **P < 0.01 vs. control).


78). Zhang T;He X;Sun L;Wang D;Zhang S;Mao J;Zhang F; et al. Insight into the Practical Models for Prediciting the Essential Role of the Cytochrome P450-mediated Biotransformation in Emodin-associated Hepatotoxicity. Toxicology 2021 Sep 4;462:152930. (PubMed: 34492313) [IF=4.099]

79). Shan L;Xu X;Zhang J;Cai P;Gao H;Lu Y;Shi J;Guo Y;Su Y; et al. Increased hemoglobin and heme in MALDI-TOF MS analysis induce ferroptosis and promote degeneration of herniated human nucleus pulposus. Mol Med 2021 Sep 8;27(1):103. (PubMed: 34496740) [IF=4.096]

80). Liu Y et al. Oligo-Porphyran Ameliorates Neurobehavioral Deficits in Parkinsonian Mice by Regulating the PI3K/Akt/Bcl-2 Pathway. Mar Drugs 2018 Mar 6;16(3) (PubMed: 29509717) [IF=4.073]

81). Xiao X et al. The miR-34a-LDHA axis regulates glucose metabolism and tumor growth in breast cancer. Sci Rep 2016 Feb 23;6:21735 (PubMed: 26902416) [IF=3.998]

Application: WB    Species:human;    Sample:breast cancer


82). Rajala A et al. Metabolic and Non-metabolic Roles of Pyruvate Kinase M2 Isoform in Diabetic Retinopathy. Sci Rep 2020 May 4;10(1):7456 (PubMed: 32366925) [IF=3.998]

83). Zhou J et al. Brusatol ameliorates 2, 4, 6-trinitrobenzenesulfonic acid-induced experimental colitis in rats: Involvement of NF-κB pathway and NLRP3 inflammasome. Int Immunopharmacol 2018 Sep 12;64:264-274 (PubMed: 30218953) [IF=3.943]

84). Zhang D et al. Wnt-3a alleviates neuroinflammation after ischemic stroke by modulating the responses of microglia/macrophages and astrocytes. Int Immunopharmacol 2019 Jul 16;75:105760 (PubMed: 31323530) [IF=3.943]

85). Tan L et al. Dihydroberberine, a hydrogenated derivative of berberine firstly identified in Phellodendri Chinese Cortex, exerts anti-inflammatory effect via dual modulation of NF-κB and MAPK signaling pathways. Int Immunopharmacol 2019 Aug 8;75:105802 (PubMed: 31401380) [IF=3.943]

86). Zhang D et al. A likely protective effect of dimethyl itaconate on cerebral ischemia/reperfusion injury. Int Immunopharmacol 2019 Oct 31;77:105924 (PubMed: 31678864) [IF=3.943]

87). Wei X;Sun C;Zhou RP;Ma GG;Yang Y;Lu C;Hu W; et al. Nerve growth factor promotes ASIC1a expression via the NF-κB pathway and enhances acid-induced chondrocyte apoptosis. Int Immunopharmacol 2020 Mar 5;82:106340. (PubMed: 32146316) [IF=3.943]

88). Liang R;Chen W;Fan H;Chen X;Zhang J;Zhu JS; et al. Dihydroartemisinin prevents dextran sodium sulphate-induced colitis through inhibition of the activation of NLRP3 inflammasome and p38 MAPK signaling. Int Immunopharmacol 2020 Sep 3;88:106949. (PubMed: 32892075) [IF=3.943]

89). Zan Liu et al. Novel circRNA_0071196/miRNA‑19b‑3p/CIT axis is associated with proliferation and migration of bladder cancer. INT J ONCOL 2020 Jul; [IF=3.899]

90). Huang X et al. High glucose disrupts autophagy lysosomal pathway in gingival epithelial cells via ATP6V0C. J Periodontol 2019 Aug 30 (PubMed: 31471894) [IF=3.742]

91). Ye QL;Wang DM;Wang X;Zhang ZQ;Tian QX;Feng SY;Zhang ZH;Yu DX;Ding DM;Xie DD; et al. Sirt1 inhibits kidney stones formation by attenuating calcium oxalate-induced cell injury. Chem Biol Interact 2021 Jul 29;347:109605. (PubMed: 34333021) [IF=3.723]

92). Ni H et al. Exosomes Derived From Bone Mesenchymal Stem Cells Ameliorate Early Inflammatory Responses Following Traumatic Brain Injury. Front Neurosci 2019 Jan 24;13:14 (PubMed: 30733666) [IF=3.707]

93). Zheng Z;Bian Y;Zhang Y;Ren G;Li G; et al. Metformin activates AMPK/SIRT1/NF-κB pathway and induces mitochondrial dysfunction to drive caspase3/GSDME-mediated cancer cell pyroptosis. Cell Cycle 2020 May;19(10):1089-1104. (PubMed: 32286137) [IF=3.699]

94). Wu M et al. Essential oils from Inula japonica and Angelicae dahuricae enhance sensitivity of MCF-7/ADR breast cancer cells to doxorubicin via multiple mechanisms. J Ethnopharmacol 2016 Mar 2;180:18-27 (PubMed: 26795076) [IF=3.690]

95). Zhang C;Li L;Hou S;Shi Z;Xu W;Wang Q;He Y;Gong Y;Fang Z;Yang Y; et al. Astragaloside IV inhibits hepatocellular carcinoma by continually suppressing the development of fibrosis and regulating pSmad3C/3L and Nrf2/HO-1 pathways. J Ethnopharmacol 2021 Jun 19;279:114350. (PubMed: 34157326) [IF=3.690]

96). Li Y;Yu P;Fu W;Cai L;Yu Y;Feng Z;Wang Y;Zhang F;Yu X;Xu H;Sui D; et al. Ginseng–Astragalus–oxymatrine injection ameliorates cyclophosphamide-induced immunosuppression in mice and enhances the immune activity of RAW264. 7 cells. J Ethnopharmacol 2021 Oct 28;279:114387. (PubMed: 34216728) [IF=3.690]

97). Tian YM et al. Anti-diabetes effect of chronic intermittent hypobaric hypoxia through improving liver insulin resistance in diabetic rats. Life Sci 2016 Apr 1;150:1-7 (PubMed: 26883978) [IF=3.647]

98). Li F et al. Icariin improves the cognitive function of APP/PS1 mice via suppressing endoplasmic reticulum stress. Life Sci 2019 Aug 7:116739 (PubMed: 31400352) [IF=3.647]

99). Peng J;Wang H;Gong Z;Li X;He L;Shen Q;Pan J;Peng Y; et al. Idebenone attenuates cerebral inflammatory injury in ischemia and reperfusion via dampening NLRP3 inflammasome activity. Mol Immunol 2020 May 18;123:74-87. (PubMed: 32438202) [IF=3.641]

100). Liu X;Qiu Y;Liu Y;Huang N;Hua C;Wang Q;Wu Z;Lu J;Song P;Xu J;Li P;Yin Y; et al. Citronellal ameliorates doxorubicin‐induced hepatotoxicity via antioxidative stress, antiapoptosis, and proangiogenesis in rats. J Biochem Mol Toxicol 2021 Feb;35(2):e22639. (PubMed: 33051984) [IF=3.606]

101). Chen Z et al. Lower Expression of Gelsolin in Colon Cancer and Its Diagnostic Value in Colon Cancer Patients. J Cancer 2019 Jan 30;10(5):1288-1296 (PubMed: 30854138) [IF=3.565]

102). Bai N et al. CircABCC2 Regulates Hepatocellular Cancer Progression by Decoying MiR-665. J Cancer 2019 Jun 10;10(17):3893-3898 (PubMed: 31417632) [IF=3.565]

103). Liu J et al. circGFRA1 Promotes Ovarian Cancer Progression By Sponging miR-449a. J Cancer 2019 Jun 24;10(17):3908-3913 (PubMed: 31417634) [IF=3.565]

104). Bai J et al. Irradiation-induced senescence of bone marrow mesenchymal stem cells aggravates osteogenic differentiation dysfunction via paracrine signaling. Am J Physiol Cell Physiol 2020 Apr 1 (PubMed: 32233952) [IF=3.485]

105). Wang DD;Li Y;Wu YJ;Wu YL;Han J;Olatunji OJ;Wang L;Zuo J; et al. Xanthones from securidaca inappendiculata antagonized the anti-rheumatic effects of methotrexate in vivo by promoting its secretion into urine. Expert Opin Drug Metab Toxicol 2020 Nov 12;1-10. (PubMed: 33107357) [IF=3.470]

106). Zhao CC;Guo H;Wang Y;Li JH; et al. Comprehensive upstream and downstream regulatory analyses identify miR-675-3p as a potential prognostic biomarker in melanoma. Hum Cell 2021 Jan 5. (PubMed: 33400243) [IF=3.463]

107). He Z;Chen D;Wu J;Sui C;Deng X;Zhang P;Chen Z;Liu D;Yu J;Shi J;Li G;Yao X; et al. Yes associated protein 1 promotes resistance to 5-fluorouracil in gastric cancer by regulating GLUT3-dependent glycometabolism reprogramming of tumor-associated macrophages. Arch Biochem Biophys 2021 May 15;702:108838. (PubMed: 33727040) [IF=3.391]

108). Ren W et al. Arginine inhibits the malignant transformation induced by interferon-gamma through the NF-κB-GCN2/eIF2α signaling pathway in mammary epithelial cells in vitro and in vivo. Exp Cell Res 2018 Jul 15;368(2):236-247 (PubMed: 29746817) [IF=3.383]

109). Lang J;Yang C;Liu L;Li L;Wu L;Liu Y;Luo H;Yan L;Chen S;Ning J;Yang C; et al. High Glucose Activates ERK1/2 to Stabilize AP1 and Increase MMP9 Expression in Diabetic Foot Ulcers. Exp Cell Res 2021 Mar 3;112550. (PubMed: 33675806) [IF=3.383]

110). Han S et al. High CCL7 expression is associated with migration, invasion and bone metastasis of non-small cell lung cancer cells. Am J Transl Res 2019 Jan 15;11(1):442-452 (PubMed: 30788000) [IF=3.375]

111). Yan J et al. The role of SIRT1 in neuroinflammation and cognitive dysfunction in aged rats after anesthesia and surgery. Am J Transl Res 2019 Mar 15;11(3):1555-1568 (PubMed: 30972182) [IF=3.375]

112). Luoreng ZM;Wei DW;Wang XP; et al. MiR-125b regulates inflammation in bovine mammary epithelial cells by targeting the NKIRAS2 gene. Vet Res 2021 Sep 17;52(1):122. (PubMed: 34535180) [IF=3.357]

113). Wu CZ et al. HMGB1/RAGE axis mediates the apoptosis, invasion, autophagy, and angiogenesis of the renal cell carcinoma. Onco Targets Ther 2018 Aug 1;11:4501-4510 (PubMed: 30122942) [IF=3.337]

114). Guan L et al. HSP90 Inhibitor Ganetespib (STA-9090) Inhibits Tumor Growth in c-Myc-Dependent Esophageal Squamous Cell Carcinoma. Onco Targets Ther 2020 Apr 8;13:2997-3011 (PubMed: 32308431) [IF=3.337]

115). Zhao M;Sun Y;Gao Z;Cui H;Chen J;Wang M;Wang Z; et al. Gigantol Attenuates the Metastasis of Human Bladder Cancer Cells, Possibly Through Wnt/EMT Signaling. Onco Targets Ther 2020 Nov 4;13:11337-11346. (PubMed: 33177841) [IF=3.337]

116). Li M;Feng L;Jiang WD;Wu P;Liu Y;Jiang J;Kuang SY;Tang L;Zhou XQ; et al. Betaine supplementations enhance the intestinal immunity of on-growing grass carp (Ctenopharyngodon idella): partly related to TOR and NF-κB signaling pathways . Br J Nutr 2020 Apr 14;123(7):737-755. (PubMed: 31831090) [IF=3.334]

117). Li M;Feng L;Jiang WD;Wu P;Liu Y;Jiang J;Kuang SY;Tang L;Zhou XQ; et al. Betaine supplementations enhance the intestinal immunity of on-growing grass carp (Ctenopharyngodon idella): partly related to TOR and NF-κB signaling pathways . Br J Nutr 2020 Apr 14;123(7):737-755. (PubMed: 31831090) [IF=3.334]

118). Zheng L et al. Selenium deficiency impaired immune function of the immune organs in young grass carp (Ctenopharyngodon idella). Fish Shellfish Immunol 2018 Jun;77:53-70 (PubMed: 29559270) [IF=3.298]

119). Zheng L et al. Selenium deficiency impaired structural integrity of the head kidney, spleen and skin in young grass carp (Ctenopharyngodon idella). Fish Shellfish Immunol 2018 Nov;82:408-420 (PubMed: 30142391) [IF=3.298]

120). Zhong JR et al. Phytic acid disrupted intestinal immune status and suppressed growth performance in on-growing grass carp (Ctenopharyngodon idella). Fish Shellfish Immunol 2019 Jun 24;92:536-551 (PubMed: 31247320) [IF=3.298]

121). Peng XR et al. Supplementation exogenous bile acid improved growth and intestinal immune function associated with NF-κB and TOR signalling pathways in on-growing grass carp (Ctenopharyngodon idella): Enhancement the effect of protein-sparing by dietary lipid. Fish Shellfish Immunol 2019 Jun 26;92:552-569 (PubMed: 31252043) [IF=3.298]

122). Liu XW;Zhang JX;Feng L;Jiang WD;Wu P;Kuang SY;Tang L;Shi HQ;Zhou XQ;Liu Y; et al. Protective effects and potential mechanisms of (2-Carboxyethyl) dimethylsulfonium Bromide (Br-DMPT) on gill health status of on-growing grass carp (Ctenopharyngodon idella) after infection with Flavobacterium columnare. Fish Shellfish Immunol 2020 Aug 6;S1050-4648(20)30517-9. (PubMed: 32771611) [IF=3.298]

123). Ma G;Li G;Gou A;Xiao Z;Xu Y;Song S;Guo K;Liu Z; et al. Long non-coding RNA ELFN1-AS1 in the pathogenesis of pancreatic cancer. Ann Transl Med 2021 May;9(10):877. (PubMed: 34164511) [IF=3.297]

124). Wang HF;Ma JX;Shang QL;An JB;Chen HT; et al. Crocetin inhibits the proliferation, migration and TGF-β 2-induced epithelial-mesenchymal transition of retinal pigment epithelial cells . Eur J Pharmacol 2017 Nov 15;815:391-398. (PubMed: 28970011) [IF=3.263]

125). Wang HF;Ma JX;Shang QL;An JB;Chen HT; et al. Crocetin inhibits the proliferation, migration and TGF-β 2-induced epithelial-mesenchymal transition of retinal pigment epithelial cells . Eur J Pharmacol 2017 Nov 15;815:391-398. (PubMed: 28970011) [IF=3.263]

Application: WB    Species:human;    Sample:Not available

Figure 2. Crocetin induces cell cycle arrest and influences PCNA, p21, and p53 expression in RPE cells. (A) Cells were harvested after treatment with or without 50 and 100 μM crocetin for 24 h. DNA was stained with PI for flow cytometric analysis. The number of cells in G1 phase was significantly increased in the crocetin-treated group compared with that in the untreated group. (B) Cells were treated or without with 50, 100 and 200 μM crocetin for 48 h. Western blot analysis was used to evaluated the expression of PCNA, p21, p53 and the housekeeping protein β-actin. *P< 0.05 vs 0 μM crocetin, **P< 0.01 vs 0 μM crocetin. The data are presented as the mean ± S.D. (n = 3/group).


126). Zhang H et al. Crocetin inhibits PDGF-BB-induced proliferation and migration of retinal pigment epithelial cells. Eur J Pharmacol 2018 Nov 2 (PubMed: 30395849) [IF=3.263]

127). Yin Q;Gu J;Qi Y;Lu Y;Yang L;Liu J;Liang X; et al. ADAM28 from both endothelium and gastric cancer cleaves von Willebrand Factor to eliminate von Willebrand Factor-induced apoptosis of gastric cancer cells. Eur J Pharmacol 2021 May 5;898:173994. (PubMed: 33675784) [IF=3.263]

128). Liu P;Zou Y;Li X;Yang A;Ye F;Zhang J;Wei W;Kong Y; et al. circGNB1 Facilitates Triple-Negative Breast Cancer Progression by Regulating miR-141-5p-IGF1R Axis. Front Genet 2020 Mar 5;11:193. (PubMed: 32194644) [IF=3.258]

129). Wen L et al. Increased expression of long noncoding RNA GAS6-AS2 promotes proliferation and inhibits apoptosis of melanoma cells via upregulating GAS6 expression. IUBMB Life 2019 Jun 4 (PubMed: 31162889) [IF=3.244]

130). LeiGan et al. Erucic acid inhibits growth performance and disrupts intestinal structural integrity of on-growing grass carp (Ctenopharyngodon idella). Aquaculture 2019;513:734437 [IF=3.224]

131). Zhang M et al. Chronic inflammation promotes proliferation in the prostatic stroma in rats with experimental autoimmune prostatitis: study for a novel method of inducing benign prostatic hyperplasia in a rat model. World J Urol 2020 Jan 21 (PubMed: 31965289) [IF=3.217]

132). Feng SH;Zhao B;Zhan X;Motanyane R;Wang SM;Li A; et al. Danggui Buxue Decoction in the Treatment of Metastatic Colon Cancer: Network Pharmacology Analysis and Experimental Validation. Drug Des Devel Ther 2021 Feb 24;15:705-720. (PubMed: 33658761) [IF=3.216]

133). Zhu L et al. miR-199b-5p Regulates Immune-Mediated Allograft Rejection after Lung Transplantation Through the GSK3β and NF-κB Pathways. Inflammation 2018 Aug;41(4):1524-1535 (PubMed: 29779167) [IF=3.212]

134). Zhu L et al. miR-199b-5p Regulates Immune-Mediated Allograft Rejection after Lung Transplantation Through the GSK3β and NF-κB Pathways. Inflammation 2018 Aug;41(4):1524-1535 (PubMed: 29779167) [IF=3.212]

135). Lu M et al. CTGF Triggers Rat Astrocyte Activation and Astrocyte-Mediated Inflammatory Response in Culture Conditions. Inflammation 2019 Jun 10 (PubMed: 31183597) [IF=3.212]

136). Chen G et al. SOSTDC1 inhibits bone metastasis in non-small cell lung cancer and may serve as a clinical therapeutic target. Int J Mol Med 2018 Oct 10 (PubMed: 30320379) [IF=3.098]

137). Wu Y et al. miR‑139‑5p affects cell proliferation, migration and adipogenesis by targeting insulin‑like growth factor 1 receptor in hemangioma stem cells. Int J Mol Med 2020 Feb;45(2):569-577 (PubMed: 31894289) [IF=3.098]

138). Guo Y;Zhu H;Li X;Ma C;Li Y;Sun T;Wang Y;Wang C;Guan W;Liu C; et al. RepSox effectively promotes the induced differentiation of sheep fibroblasts into adipocytes via the inhibition of the TGF‑β1/Smad pathway. Int J Mol Med 2021 Aug;48(2):148. (PubMed: 34132357) [IF=3.098]

139). Liu YL;Yang WH;Chen BY;Nie J;Su ZR;Zheng JN;Gong ST;Chen JN;Jiang D;Li Y; et al. miR‑29b suppresses proliferation and induces apoptosis of hepatocellular carcinoma ascites H22 cells via regulating TGF‑β1 and p53 signaling pathway. Int J Mol Med 2021 Aug;48(2):157. (PubMed: 34184070) [IF=3.098]

140). Wang X;Shi Z;Zhao Q;Yun Y; et al. Study on the Structure and Properties of Biofunctional Keratin from Rabbit Hair. Materials (Basel) 2021 Jan 14;14(2):E379. (PubMed: 33466740) [IF=3.057]

141). Lu Z;Zhang D;Cui K;Fu X;Man J;Lu H;Yu L;Gao Y;Liu X;Liao L;Li X;Liu C;Zhang Y;Zhang Z;Wang J; et al. Neuroprotective action of teriflunomide in a mouse model of transient middle cerebral artery occlusion. Neuroscience 2020 Jan 21;428:228-241. (PubMed: 31887363) [IF=3.056]

142). Liu DH et al. Anticonvulsant and Neuroprotective Effects of Paeonol in Epileptic Rats. Neurochem Res 2019 Sep 13 (PubMed: 31520267) [IF=3.038]

143). Wan LY et al. miR-320 enhances the sensitivity of human colon cancer cells to chemoradiotherapy in vitro by targeting FOXM1. Biochem Biophys Res Commun 2015 Feb 6;457(2):125-32 (PubMed: 25446103)

Application: WB    Species:Not available;    Sample:Not available


144). Wu Z et al. MFAP5 promotes tumor progression and bone metastasis by regulating ERK/MMP signaling pathways in breast cancer. Biochem Biophys Res Commun 2018 Apr 6;498(3):495-501 (PubMed: 29526753)

145). Xin Y et al. Versatile Pt NCs-based chemotherapeutic agents significantly induce the apoptosis of cisplatin-resistant non-small cell lung cancer. Biochem Biophys Res Commun 2019 Apr 30;512(2):218-223 (PubMed: 30885437)

146). Lei X et al. Effects of cyclic fluid stress at different frequencies on behaviors of cells incubated on titanium alloy. Biochem Biophys Res Commun 2020 Jan 29;522(1):100-106 (PubMed: 31740003)

147). Zhang N;Liu C;Zhang Y;Xu D;Gui L;Lu Y;Zhang Q; et al. Liraglutide regulates lipid metabolism via FGF21-LKB1-AMPK-ACC1 pathway in white adipose tissues and macrophage of type 2 diabetic mice. Biochem Biophys Res Commun 2021 Feb 25;548:120-126. (PubMed: 33640604)

148). Li L et al. MiR-93-5p promotes gastric cancer-cell progression via inactivation of the Hippo signaling pathway. Gene 2018 Jan 30;641:240-247 (PubMed: 29045821)

Application: WB    Species:human;    Sample:Not available

(C) miR-93-5p overexpression suppressed and increased E-cadherin and N-cadherin expression, respectively. The opposite result was observed in response to miR-93-5p downregulation.


149). Li L et al. MiR-93-5p promotes gastric cancer-cell progression via inactivation of the Hippo signaling pathway. Gene 2018 Jan 30;641:240-247 (PubMed: 29045821)

150). Chi S et al. Knockdown of long non-coding HOTAIR enhances the sensitivity to progesterone in endometrial cancer by epigenetic regulation of progesterone receptor isoform B. Cancer Chemother Pharmacol 2019 Feb;83(2):277-287 (PubMed: 30443761)

151). Liang H;Huang H;Li Y;Lu Y;Ye T; et al. CircRNA_0058063 functions as a ceRNA in bladder cancer progression via targeting miR-486-3p/FOXP4 axi s. Biosci Rep 2020 Mar 27;40(3):BSR20193484. (PubMed: 32181485)

152). Deng L;Ou Z;Huang D;Li C;Lu Z;Liu W;Wu F;Nong C;Gao J;Peng Y; et al. Diverse effects of different Akkermansia muciniphila genotypes on Brown adipose tissue inflammation and whitening in a high-fat-diet murine model. Microb Pathog 2020 Jun 24;104353. (PubMed: 32592821)

153). Zhu C et al. Downregulation of Proline Hydroxylase 2 and Upregulation of Hypoxia-Inducible Factor 1α are Associated with Endometrial Cancer Aggressiveness. Cancer Manag Res 2019 Nov 22;11:9907-9912 (PubMed: 31819628)

154). Huang J;Shen C;Ye R;Shi Y;Li W; et al. The Effect of Early Maternal Separation Combined With Adolescent Chronic Unpredictable Mild Stress on Behavior and Synaptic Plasticity in Adult Female Rats. Front Psychiatry 2021 Mar 5;12:539299. (PubMed: 33746787)

155). Chen W et al. MicroRNA-214 protects L6 skeletal myoblasts against hydrogen peroxide-induced apoptosis. Free Radic Res 2020 Mar 5:1-11 (PubMed: 32131653)

156). Locatelli FM et al. Resveratrol-loaded nanoemulsion prevents cognitive decline after abdominal surgery in aged rats. J Pharmacol Sci 2018 Aug;137(4):395-402 (PubMed: 30196020)

157). Shang J et al. CircPAN3 mediates drug resistance in acute myeloid leukemia through the miR-153-5p/miR-183-5p-XIAP axis. Exp Hematol 2018 Nov 2 (PubMed: 30395908)

158). Ding S et al. Combined effects of ambient particulate matter exposure and a high-fat diet on oxidative stress and steatohepatitis in mice. PLoS One 2019 Mar 28;14(3):e0214680 (PubMed: 30921449)

159). Zhang W et al. Microencapsulated olfactory ensheathing cell transplantation reduces P2X4 receptor overexpression and inhibits neuropathic pain in rats. Brain Res 2019 Sep 17:146465 (PubMed: 31539546)

160). Ge QD et al. Differential Expression of miRNAs in the Hippocampi of Offspring Rats Exposed to Fluorine Combined with Aluminum during the Embryonic Stage and into Adulthood. Biol Trace Elem Res 2018 Jul 22 (PubMed: 30033483)

161). Gan H et al. KIF2C exerts an oncogenic role in nonsmall cell lung cancer and is negatively regulated by miR-325-3p. Cell Biochem Funct 2019 Jul 22 (PubMed: 31328811)

162). Ma YD;Cui ZH;Zhao D;Wang YC;Zhang LX;Zhang XQ;Guo WJ;Yuan DZ;Zhang JH;Yue LM;Nie L; et al. The Effects of Altered Endometrial Glucose Homeostasis on Embryo Implantation in Type 2 Diabetic Mice. Reprod Sci 2020 Nov 5. (PubMed: 33151524)

163). Gao Y et al. TGF-β1 promotes bovine mammary fibroblast proliferation through the ERK 1/2 signalling pathway. Cell Biol Int 2016 Jul;40(7):750-60 (PubMed: 27063575)

Application: WB    Species:Not available;    Sample:Not available


164). Wang JL;Chen WG;Zhang JJ;Xu CJ; et al. Nogo-A-Δ20/EphA4 interaction antagonizes apoptosis of neural stem cells by integrating p38 and JNK MAPK signaling. J Mol Histol 2021 Feb 8. (PubMed: 33555537)

165). Wang Y et al. The Role of YB1 in Renal Cell Carcinoma Cell Adhesion. Int J Med Sci 2018 Aug 6;15(12):1304-1311 (PubMed: 30275756)

166). Wang Y et al. The Role of YB1 in Renal Cell Carcinoma Cell Adhesion. Int J Med Sci 2018 Aug 6;15(12):1304-1311 (PubMed: 30275756)

167). Cao L;Zhang X;Ji B;Ding S;Qi Z; et al. Moderate endurance training reduced hepatic tumourigenesis associated with lower lactate overload compared to high‐intensity interval training. Clin Exp Pharmacol Physiol 2021 Jun 6. (PubMed: 34096088)

168). Lei Z et al. Proteomic Analysis of Beef Tenderloin and Flank Assessed Using an Isobaric Tag for Relative and Absolute Quantitation (iTRAQ). Animals (Basel) 2020 Jan 16;10(1) (PubMed: 31963250)

169). Gong T;Wang W;Xu H;Yang Y;Chen X;Meng L;Xu Y;Li Z;Wan S;Mu Q; et al. Longitudinal Expression of Testicular TAS1R3 from Prepuberty to Sexual Maturity in Congjiang Xiang Pigs. Animals (Basel) 2021 Feb 8;11(2):437. (PubMed: 33567555)

170). Gong T;Wang W;Xu H;Yang Y;Chen X;Meng L;Xu Y;Li Z;Wan S;Mu Q; et al. Longitudinal Expression of Testicular TAS1R3 from Prepuberty to Sexual Maturity in Congjiang Xiang Pigs. Animals (Basel) 2021 Feb 8;11(2):437. (PubMed: 33567555)

171). Hu Y et al. Expression and clinical relevance of SPOPL in medulloblastoma. Oncol Lett 2017 Sep;14(3):3051-3056 (PubMed: 28928843)

Application: WB    Species:human;    Sample:Not available


172). Wu K et al. Antitumor effect of ginsenoside Rg3 on gallbladder cancer by inducing endoplasmic reticulum stress-mediated apoptosis in vitro and in vivo. Oncol Lett 2018 Nov;16(5):5687-5696 (PubMed: 30344724)

173). Cai W et al. Uric Acid Induces Endothelial Dysfunction by Activating the HMGB1/RAGE Signaling Pathway. Biomed Res Int 2017;2017:4391920 (PubMed: 28116308)

Application: WB    Species:human;    Sample:Not available


174). Wang X;Zeng HC;Huang YR;He QZ; et al. Chlamydia muridarum Alleviates Colitis via the IL-22/Occludin Signal Pathway. Biomed Res Int 2020 Dec 17;2020:8894331. (PubMed: 33381598)

175). Zhang W et al. Effects of microencapsulated olfactory ensheathing cell transplantation on neuropathic pain and P2X7 receptor expression in the L4-5 spinal cord segment. Neurosci Lett 2019 Feb 9 (PubMed: 30753909)

176). Zhang WJ;Luo HL;Zhu ZM; et al. Effect of olfactory ensheathing cells combined with chitosan on inhibition of P2× 4 receptor over-expression-mediated neuropathic pain. Neurosci Lett 2020 Mar 23;722:134859. (PubMed: 32097702)

177). Li J;Kong L;Huang H;Luan S;Jin R;Wu F; et al. ASIC1a inhibits cell pyroptosis induced by acid‐induced activation of rat hepatic stellate cells. FEBS Open Bio 2020 Jun;10(6):1044-1055. (PubMed: 32237041)

178). Wang J et al. Interfering Expression of Chimeric Transcript SEPT7P2-PSPH Promotes Cell Proliferation in Patients with Nasopharyngeal Carcinoma. J Oncol 2019 Apr 1;2019:1654724 (PubMed: 31057610)

179). Zhang S;Zhao J;Ma W; et al. Circ-Sirt1 inhibits proliferation, induces apoptosis, and ameliorates inflammation in human rheumatoid arthritis fibroblast-like synoviocytes. Autoimmunity 2021 Aug 25;1-12. (PubMed: 34431434)

180). Zhang WJ et al. Transplantation of microencapsulated olfactory ensheathing cells inhibits the P2X2 receptor over-expression mediated neuropathic pain in the L4-5 spinal cord segment. Int J Neurosci 2020 Jan 8:1-10 (PubMed: 31914345)

181). Feng Y et al. Neuroprotective effects of resveratrol against traumatic brain injury in rats: Involvement of synaptic proteins and neuronal autophagy. Mol Med Rep 2016 Jun;13(6):5248-54 (PubMed: 27122047)

182). Feng Y et al. Bone marrow stromal cells promote neuromotor functional recovery, via upregulation of neurotrophic factors and synapse proteins following traumatic brain injury in rats. Mol Med Rep 2017 Jul;16(1):654-660 (PubMed: 28560414)

183). Zhang G et al. Acute stimulatory effect of tumor necrosis factor on the basolateral 50 pS K channels in the thick ascending limb of the rat kidney. Mol Med Rep 2018 Sep 10 (PubMed: 30221721)

184). Jia J;Sun J;Liao W;Qin L;Su K;He Y;Zhang J;Yang R;Zhang Z;Sun Y; et al. Knockdown of long non‑coding RNA AK094629 attenuates the interleukin‑1β induced expression of interleukin‑6 in synovium‑derived mesenchymal stem cells from the temporomandibular joint. Mol Med Rep 2020 May 28. (PubMed: 32468015)

185). Chen Y;Wei B;Xu P;Tang H;Yang L;Wang Y;Fu Y;Yang X;Mao Y; et al. Schistosoma japonicum cystatin suppresses osteoclastogenesis via manipulating the NF‑κB signaling pathway. Mol Med Rep 2021 Apr;23(4):273. (PubMed: 33576450)

186). Zhu Y;Su Y;Zhang J;Zhang Y;Li Y;Han Y;Dong X;Li W;Li W; et al. Astragaloside IV alleviates liver injury in type 2 diabetes due to promotion of AMPK/mTOR‑mediated autophagy. Mol Med Rep 2021 Jun;23(6):437. (PubMed: 33846768)

187). Ren WB et al. Interferon-γ regulates cell malignant growth via the c-Abl/HDAC2 signaling pathway in mammary epithelial cells. J Zhejiang Univ Sci B 2019 Jan (PubMed: 30614229)

188). Feifei W et al. MAGP2, a Component of Extracellular Matrix, Is Upregulated in Colorectal Cancer and Negatively Modulated by miR-200b-3p. Technol Cancer Res Treat 2019 Jan 1;18:1533033819870777 (PubMed: 31426719)

189). Li Y et al. Theaflavic acid from black tea protects PC12 cells against ROS-mediated mitochondrial apoptosis induced by OGD/R via activating Nrf2/ARE signaling pathway. J Nat Med 2019 Jun 21 (PubMed: 31227974)

190). Han J;Shi G;Li W;Xie Y;Li F;Jiang D; et al. Preventive effect of dioscin against monosodium urate-mediated gouty arthritis through inhibiting inflammasome NLRP3 and TLR4/NF-κB signaling pathway activation: an in vivo and in vitro study. J Nat Med 2020 Aug 6. (PubMed: 32761488)

191). Yang C et al. Expression of glucose-regulated protein 78 as prognostic biomarkers for triple-negative breast cancer. Histol Histopathol 2019 Nov 19:18185 (PubMed: 31745967)

192). Chen Y;Ding Y;Wang LM; et al. Tripartite motif-containing 35 (TRIM35) is up-regulated in UUO-induced renal fibrosis animal model. Histol Histopathol 2020 Sep 21;18255. (PubMed: 32955098)

193). Lu Y et al. Lycopene alleviates disc degeneration under oxidative stress through the Nrf2 signaling pathway. Mol Cell Probes 2020 Mar 6:101559 (PubMed: 32151764)

194). Huang H et al. Astragaloside IV protects cardiomyocytes from anoxia/reoxygenation injury by upregulating the expression of Hes1 protein. Can J Physiol Pharmacol 2016 May;94(5):542-53 (PubMed: 27070866)

195). Wu X et al. Antioxidative and Anti-Inflammatory Effects of Water Extract of Acrostichum aureum Linn. against Ethanol-Induced Gastric Ulcer in Rats. Evid Based Complement Alternat Med 2018 Dec 12;2018:3585394 (PubMed: 30643529)

196). Zhong W et al. Camellia (Camellia oleifera Abel.) seed oil promotes milk fat and protein synthesis-related gene expression in bovine mammary epithelial cells. Food Sci Nutr 2019 Dec 5;8(1):419-427 (PubMed: 31993168)

197). Song C et al. Sericin enhances the insulin-PI3K/AKT signaling pathway in the liver of a type 2 diabetes rat model. Exp Ther Med 2018 Oct;16(4):3345-3352 (PubMed: 30250521)

198). Yang L et al. Angiogenic function of astragaloside IV in rats with myocardial infarction occurs via the PKD1-HDAC5-VEGF pathway. Exp Ther Med 2019 Apr;17(4):2511-2518 (PubMed: 30906439)

199). Wang W;Zheng S;He H;Ge H;Saeed BR; et al. N, N‑diethylaminobenzaldehyde targets aldehyde dehydrogenase to eradicate human pancreatic cancer cells. Exp Ther Med 2020 Jul;20(1):662-670. (PubMed: 32550888)

200). Liu YL;Chen BY;Nie J;Zhao GH;Zhuo JY;Yuan J;Li YC;Wang LL;Chen ZW; et al. Polydatin prevents bleomycin‑induced pulmonary fibrosis by inhibiting the TGF‑β/Smad/ERK signaling pathway. Exp Ther Med 2020 Nov;20(5):62. (PubMed: 32952652)

201). Gong N;Yang X;Li X;Jiang Y;Gu C;Ma S;Gao Q;Cheng X; et al. MicroRNA‑590‑3p relieves hypoxia/reoxygenation induced cardiomyocytes apoptosis and autophagy by targeting HIF‑1α. Exp Ther Med 2021 Oct;22(4):1077. (PubMed: 34447470)

202). Huankai Yao et al. Protective effects of hederagenic acid on PC12 cells against the OGD/R-induced apoptosis via activating Nrf2/ARE signaling pathway. Med Chem Res 2020 Jan

203). He J;He L;Lu F;Geng B;Xia Y; et al. Low-molecular-weight heparin calcium attenuates the tourniquet-induced ischemia-reperfusion injury in rats. Injury 2021 Mar 5;S0020-1383(21)00221-7. (PubMed: 33726921)

204). Tang C;Tao J;Sun J;Lv F;Lu Z;Lu Y; et al. Regulatory mechanisms of energy metabolism and inflammation in oleic acid‐treated HepG2 cells from Lactobacillus acidophilus NX2‐6 extract. J Food Biochem 2021 Sep 5;e13925. (PubMed: 34486133)

205). Li H et al. Impact of Taurine on the proliferation and apoptosis of human cervical carcinoma cells and its mechanism. Chin Med J (Engl) 2019 Apr 20;132(8):948-956 (PubMed: 30958437)

206). Zhou Y;Cheng Y;Li Y;Ma J;Wu Z;Chen Y;Mei J;Chen M; et al. Soluble β-amyloid impaired the GABA inhibition by mediating KCC2 in early APP/PS1 mice. Biosci Trends 2021 Sep 16. (PubMed: 34526443)

207). Yu J;Chen WX;Xie WJ;Chen RW;Lin DQ;You WW;Ye WL;Zhang HQ;Lin DH;Xu JP; et al. Silencing of the CrkL gene reverses the doxorubicin resistance of K562/ADR cells through regulating PI3K/Akt/MRP1 signaling. J Clin Lab Anal 2021 Jun 11;e23817. (PubMed: 34114685)

208). Chen M et al. The Therapeutic Effects and Possible Mechanism of Pranoprofen in Mouse Model of Corneal Alkali Burns. J Ophthalmol 2020 Apr 6;2020:7485912 (PubMed: 32322412)

209). Han H et al. Lamotrigine attenuates cerebral ischemia-induced cognitive impairment and decreases β-amyloid and phosphorylated tau in the hippocampus in rats. Neuroreport 2015 Aug 19;26(12):723-7 (PubMed: 26164461)

Application: WB    Species:rat;    Sample:rat

LTG decreased Aβ1–42 and phosphorylated tau protein levels in the ipsilateral hippocampus of MCAO rats. (a) Immunoblotting photographs of Aβ1–42, Tau-5, and AT8. (b–d) Quantitative analysis of immunoreactivity of Aβ1–42 (b), Tau-5 (c), and AT8 (d). (e) Immunofluorescence of the hippocampal CA1 zone for AT8. Scale bar=50 μm. *Means sham versus another group, *P<0.05, **P< 0.01, ***P<0.001; † LTG 20 versus vehicle, † P<0.05, †††P<0.001; ‡ LTG 40 versus vehicle, ‡‡P< 0.01, ‡‡‡P


210). Zhou J;Zhang D;Bai J;Li Z;Chen Y; et al. Altered Expressions of AQP3 and ADP Are Closely Related with the Risk of Preeclampsia Occurrence. Gynecol Obstet Invest 2020 Sep 21;85(4):362-370. (PubMed: 32957104)

211). Plaza A et al. Inflammatory stress and altered angiogenesis evoked by very high-fat diets in mouse liver. Endocrinol Diabetes Nutr 2019 Aug - Sep;66(7):434-442 (PubMed: 30833154)

212). Tang WJ;Hu WH;Huang Y;Wu BB;Peng XM;Zhai XW;Qian XW;Ye ZQ;Xia HJ;Wu J;Shi JR; et al. Potential protein–phenotype correlation in three lipopolysaccharide-responsive beige-like anchor protein-deficient patients. World J Clin Cases 2021 Jul 26;9(21):5873-5888. (PubMed: 34368306)

213). Chen J;Liu J;Wu S;Liu W;Xia Y;Zhao J;Yang Y;Wang Y;Peng Y;Zhao S; et al. Atrazine Promoted Epithelial Ovarian Cancer Cells Proliferation and Metastasis by Inducing Low Dose Reactive Oxygen Species (ROS). Iran J Biotechnol 2021 Apr 1;19(2):e2623. (PubMed: 34435054)

214). Huikun Xu et al. Chemerin promotes the viability and migration of human placental microvascular endothelial cells and activates MAPK/AKT signaling . Int J Clin Exp Med 2018;11(2):721-727

215). et al. Effects of Sancai Lianmei Particle on autophagy and apoptosis in testes of diabetic mice via the Nrf2/HO-1 pathway. Int J Clin Exp Med 2019;12(6):6720-6732

216). Qi H et al. Histone Demethylase JMJD2A Inhibition Attenuates Neointimal Hyperplasia in the Carotid Arteries of Balloon-Injured Diabetic Rats via Transcriptional Silencing: Inflammatory Gene Expression in Vascular Smooth Muscle Cells. Cell Physiol Biochem 2015;37(2):719-34 (PubMed: 26356263)

Application: WB    Species:Not available;    Sample:Not available


217). Li XH et al. Parthenolide attenuated bleomycin-induced pulmonary fibrosis via the NF-κB/Snail signaling pathway. Respir Res 2018 Jun 5;19(1):111 (PubMed: 29871641)

218). Liu J et al. Irisin Enhances Doxorubicin-Induced Cell Apoptosis in Pancreatic Cancer by Inhibiting the PI3K/AKT/NF-κB Pathway. Med Sci Monit 2019 Aug 14;25:6085-6096 (PubMed: 31412018)

219). Xiangli Yan et al. Calycosin-7-O-β-D-glucoside Attenuates OGD/R-Induced Damage by Preventing Oxidative Stress and Neuronal Apoptosis via the SIRT1/FOXO1/PGC-1α Pathway in HT22 Cells. NEURAL PLAST 2019, Article ID 8798069, 11 pages

220). Du P;Hu T;An Z;Li P;Liu L; et al. In vitro and in vivo synergistic efficacy of ceritinib combined with programmed cell death ligand‐1 inhibitor in anaplastic lymphoma kinase‐rearranged non‐small‐cell lung cancer. Cancer Sci 2020 Jun;111(6):1887-1898. (PubMed: 32227409)

221). Wang T;Lu H;Li D;Huang W; et al. TGF-β1-Mediated Activation of SERPINE1 is Involved in Hemin-Induced Apoptotic and Inflammatory Injury in HT22 Cells. Neuropsychiatr Dis Treat 2021 Feb 11;17:423-433. (PubMed: 33603380)

222). Zhao CC;Guo H;Wang Y;Li JH; et al. Comprehensive upstream and downstream regulatory analyses identify miR-675-3p as a potential prognostic biomarker in melanoma. Hum Cell 2021 Jan 5. (PubMed: 33400243)

223). Jiang D;Mo Q;Sun X;Wang X;Dong M;Zhang G;Chen F;Zhao Q; et al. Pyruvate dehydrogenase kinase 4‐mediated metabolic reprogramming is involved in rituximab resistance in diffuse large B‐cell lymphoma by affecting the expression of MS4A1/CD20. Cancer Sci 2021 Sep;112(9):3585-3597. (PubMed: 34252986)

224). Zhang Y;Dou S;Qi X;Zhang Z;Qiao Y;Wang Y;Xie J;Jiang H;Zhang B;Zhou Q;Wang Q;Xie L; et al. Transcriptional Network Analysis Reveals the Role of miR-223-5p During Diabetic Corneal Epithelial Regeneration. Front Mol Biosci 2021 Aug 26;8:737472. (PubMed: 34513931)

225). Gong Y et al. CUL4A promotes cell invasion in gastric cancer by activating the NF-κB signaling pathway. Biologics 2017 Apr 12;11:45-53 (PubMed: 28442889)

Application: WB    Species:human;    Sample:HGC27

Figure 4 CUL4A and NF-κB were overexpressed in GC tissues. Notes: (A) Representative images of gastric tumor tissues showing concordant positive staining of CUL4A and NF-κB in the same sample (200×). (B) Western blot analysis of CUL4A and NF-κB expressions in three paired primary GC and adjacent noncancerous tissue samples. These three patients were all diagnosed stage III. (C) CUL4A expression scores and NF-κB expression scores in 50 GC samples revealed that CUL4A expression positively correlated with NF-κB expression via correlation analysis


226). Huang X et al. PDL1 And LDHA act as ceRNAs in triple negative breast cancer by regulating miR-34a. J Exp Clin Cancer Res 2017 Sep 15;36(1):129 (PubMed: 28915924)

227). Zhuoqi Liu et al. Effect of targeted mammalian sterile 20-like kinase 1 regulation on proliferation and apoptosis of SW480 colorectal cancer cells. Biomedical Research 2017 Jun 8;28(15)

228). Bai N et al. circFBLIM1 act as a ceRNA to promote hepatocellular cancer progression by sponging miR-346. J Exp Clin Cancer Res 2018 Jul 27;37(1):172 (PubMed: 30053867)

229). Ren WB et al. Interferon-γ regulates cell malignant growth via the c-Abl/HDAC2 signaling pathway in mammary epithelial cells. J Zhejiang Univ Sci B 2019 Jan (PubMed: 30614229)

230). Deng J et al. MicroRNA-506 inhibits gastric cancer proliferation and invasion by directly targeting Yap1. Tumour Biol 2015 Sep;36(9):6823-31 (PubMed: 25846731)

231). Yu Gong;Meijiao Song;Jianguo Zhao;Jianping Xiong;Xiaojun Xiang;Ling Zhang;Feng Yu;Jun Chen;Miao Feng;Zhengyu Zhan et al. Targeting CUL4A inhibits HCT-116 colon cancer cell proliferation and invasion in vitro and in vivo. Oncology Letters

232). et al. Protective effect of Schisandra chinensis total lignans on acute alcoholic-induced liver injury related to inhibiting CYP2E1 activation and activating the Nrf2/ARE signaling pathway.

233). et al. Effects and Mechanisms of Vitamin C Post-Conditioning on Platelet Activation after Hypoxia/Reoxygenation.

234). et al. Inflammatory stress and altered angiogenesis evoked by very high-fat diets in mouse liverLas dietas ricas en grasa estimulan procesos inflamatorios y angiogénicos en hígado de ratón.

235). et al. Erucic acid impairs intestinal immune function of on-growing grass carp (Ctenopharyngodon idella).

236). et al. Mannan oligosaccharides improved growth performance and antioxidant capacity in the intestine of on-growing grass carp (Ctenopharyngodon idella).

237). et al. Synthesis and Biological Evaluation of HDAC Inhibitors With a Novel Zinc Binding Group.

238). et al. NUSAP1 Promotes Gastric Cancer Progression Through Regulation of Yap Stability.

239). et al. Soybean glycinin caused NADPH-oxidase-regulated ROS overproduction and decreased ROS elimination capacity in the mid and distal intestine of juvenile grass carp (Ctenopharyngodon idella).

240). et al. Activation of cannabinoid receptor 2 protects rat hippocampal neurons against Aβ-induced neuronal toxicity.

241). et al. Improving the in vivo bioavailability and in vitro anti-inflammatory activity of tanshinone IIA by alginate solid dispersion.

242). et al. The role of long noncoding RNA AL161431. 1 in the development and progression of pancreatic cancer.

243). Fang CC;Feng L;Jiang WD;Wu P;Liu Y;Kuang SY;Tang L;Liu XA;Zhou XQ; et al. Effects of dietary methionine on growth performance, muscle nutritive deposition, muscle fibre growth and type I collagen synthesis of on-growing grass carp (Ctenopharyngodon idella). Br J Nutr 2020 Jul 28;1-36. (PubMed: 32718370)

244). Mo C;Xie S;Zhong W;Zeng T;Huang S;Lai Y;Deng G;Zhou C;Yan W;Chen Y;Huang S;Gao L;Lv Z; et al. Mutual antagonism between indoleamine 2, 3-dioxygenase 1 and nuclear factor E2-related factor 2 regulates the maturation status of DCs in liver fibrosis. Free Radic Biol Med 2020 Aug 6;S0891-5849(20)31185-0. (PubMed: 32771520)

245). et al. Interleukin 17A deficiency alleviates fluoride-induced testicular injury by inhibiting the immune response and apoptosis.

246). et al. Protective effects of Lactobacillus acidophilus NX2-6 against oleic acid-induced steatosis, mitochondrial dysfunction, endoplasmic reticulum stress and inflammatory responses.

247). et al. Calycosin-7-O-β-D-Glucoside Treatment Promotes Axonal Regeneration via Rho/ROCK Pathway After Ischemia/Reperfusion Injury.

248). et al. Effect of sericin on the p38MAPK signaling pathway and NLRP3 inflammasome in the kidney of type 2 diabetic rats.

249). et al. Apremilast prevents IL‑17‑induced cellular senescence in ATDC5 chondrocytes mediated by SIRT1.

250). et al. Insulin Receptor Substrate p53 Ameliorates High-Glucose-Induced Activation of NF-κB and Impaired Mobility of HUVECs.

251). et al. Kiwi Root Extract Inhibits the Development of Endometriosis in Mice by Downregulating Inflammatory Factors.

252). et al. Dietary nucleotides in the diets of on-growing grass carp (Ctenopharyngodon idella) suppress Aeromonas hydrophila induced intestinal inflammation and enhance intestinal disease-resistance via NF-κB and TOR signaling.

253). et al. Fecal Microbiota Transplantation Is a Promising Method to Restore Gut Microbiota Dysbiosis and Relieve Neurological Deficits after Traumatic Brain Injury.

254). Liang R;Chen W;Chen XY;Fan HN;Zhang J;Zhu JS; et al. Dihydroartemisinin inhibits the tumorigenesis and invasion of gastric cancer by regulating STAT1/KDR/MMP9 and P53/BCL2L1/CASP3/7 pathways. Pathol Res Pract 2021 Feb;218:153318. (PubMed: 33370709)

255). Xing Y;Wang Q;Zhang J;Li W;Duan A;Yang J;Liu Z; et al. Chromatin accessibility of kidney tubule cells under stress reveals key transcription factor mediating acute and chronic kidney disease. FEBS J 2021 Mar 13. (PubMed: 33713542)

256). et al. Heme-induced ferroptosis promotes human lumbar disc degeneration analyzed by MALDI-TOF MS.

257). et al. Study on the Structure and Properties of Biofunctional Keratin from Rabbit Hair. Materials 2021, 14, 379.

258). et al. Fractalkine Aggravates LPS-induced Macrophage Activation and Acute Kidney Injury via Wnt/β-catenin Signaling Pathway.

259). et al. Toxicity Investigation of Nano-SiO2 on Male Reproductive System in Pubertal Mice.

260). et al. ERRα Expression in Ovarian Cancer and Promotes Ovarian Cancer Cells Migration in Vitro.

261). et al. PDK4-Mediated Metabolic Reprogramming Promotes Rituximab Resistance in Diffuse Large B-Cell Lymphoma Via Negative Regulation of MS4A1/CD20.

262). et al. 10‐Gingerol alleviates hypoxia/reoxygenation‐induced cardiomyocyte injury through inhibition of the Wnt5a/Frizzled‐2 pathway.

263). Chen Y;Wen J;Chen Z; et al. H2S protects hippocampal neurons against hypoxia-reoxygenation injury by promoting RhoA phosphorylation at Ser188. Cell Death Discov 2021 Jun 4;7(1):132. (PubMed: 34088899)

264). et al. P38α Deficiency in Macrophages Ameliorates Murine Experimental Colitis by Regulating Inflammation and Immune Process.

265). et al. IGF-1 Promotes Epithelial-Mesenchymal Transition of Lens Epithelial Cells That is Conferred by miR-3666 Loss.

266). et al. Chidamide-Induced Accumulation of Reactive Oxygen Species Increases Lenalidomide Sensitivity Against Multiple Myeloma Cells.

267). et al. Cerebroprotein hydrolysate‐I protects senescence‐induced by D‐galactose in PC12 cells and mice.

268). et al. miR-129-5p Promotes Osteogenic Differentiation of BMSCs and Bone Regeneration via Repressing Dkk3.

269). Wu S;Shao M;Zhang Y;Shi D; et al. Activation of RSK2 upregulates SOX8 to promote methotrexate resistance in gestational trophoblastic neoplasia. Lab Invest 2021 Aug 9. (PubMed: 34373588)

270). et al. Therapeutic Effect of Macrophage-Derived Biomimetic Nanoparticles for Cytokine Release Syndrome.

271). et al. Chidamide, a subtype-selective histone deacetylase inhibitor, enhances Bortezomib effects in multiple myeloma therapy.

272). et al. Peptides released from bovine α-lactalbumin by simulated digestion alleviated free fatty acids-induced lipid accumulation in HepG2 cells.

273). Zhang K;Guo MY;Li QG;Wang XH;Wan YY;Yang ZJ;He M;Yi YM;Jiang LP;Qu XH;Han XJ; et al. Drp1-dependent mitochondrial fission mediates corneal injury induced by alkali burn. Free Radic Biol Med 2021 Sep 22;S0891-5849(21)00739-5. (PubMed: 34562609)

274). et al. Effects of Macamide B on the Expression of SIRT1 in Brain Tissue of Neonatal Mice with Hypoxic-ischemic Brain Damage.

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Catalog Number :

AF7018-BP
(Blocking peptide available as AF7018-BP)

Price/Size :

$350/1mg.
Tips: For phospho antibody, we provide phospho peptide(0.5mg) and non-phospho peptide(0.5mg).

Function :

Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. This mechanism is useful when non-specific binding is an issue, for example, in Western blotting (immunoblot) and immunohistochemistry (IHC). By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific; Specific binding will be absent from the western blot or immunostaining performed with the neutralized antibody.

Format and storage :

Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 10 mg/ml.The purity is >90%,tested by HPLC and MS.Storage Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C.

Precautions :

This product is for research use only. Not for use in diagnostic or therapeutic procedures.

Pig
100%
Horse
100%
Bovine
100%
Sheep
100%
Dog
100%
Xenopus
100%
Chicken
100%
Rabbit
100%
Zebrafish
90%
High similarity Medium similarity Low similarity No similarity
P60709 as Substrate
Site PTM Type Enzyme
M1 Acetylation
D2 Acetylation
S14 Phosphorylation
C17 S-Nitrosylation
K18 Methylation
K18 Ubiquitination
S33 Phosphorylation
K50 Acetylation
K50 Methylation
K50 Ubiquitination
S52 Phosphorylation
Y53 Phosphorylation
S60 Phosphorylation
K61 Acetylation
K61 Sumoylation
K61 Ubiquitination
T66 Phosphorylation
K68 Methylation
K68 Sumoylation
Y69 Phosphorylation
H73 Methylation
T77 Phosphorylation
K84 Methylation
K84 Sumoylation
K84 Ubiquitination
T89 Phosphorylation
Y91 Phosphorylation
T106 Phosphorylation
K113 Acetylation
K113 Sumoylation
K113 Ubiquitination
T120 Phosphorylation
Y143 Phosphorylation
S155 Phosphorylation
T160 Phosphorylation
T162 Phosphorylation
Y166 Phosphorylation
Y169 Phosphorylation
T186 Phosphorylation
Y188 Phosphorylation
K191 Acetylation
K191 Methylation
K191 Ubiquitination
T194 Phosphorylation
Y198 Phosphorylation
S199 Phosphorylation
T201 Phosphorylation
T202 Phosphorylation
T203 Phosphorylation
K213 Acetylation
K213 Ubiquitination
K215 Ubiquitination
C217 S-Nitrosylation
Y218 Phosphorylation
T229 Phosphorylation
S233 Phosphorylation
S235 Phosphorylation
K238 Ubiquitination
S239 Phosphorylation
Y240 Phosphorylation
T249 Phosphorylation
C257 S-Nitrosylation
S265 Phosphorylation
S271 Phosphorylation
C272 S-Nitrosylation
K284 Sumoylation
K284 Ubiquitination
C285 S-Nitrosylation
K291 Sumoylation
K291 Ubiquitination
Y294 Phosphorylation
T297 Phosphorylation
S300 Phosphorylation
T303 Phosphorylation
T304 Phosphorylation
Y306 Phosphorylation
K315 Acetylation
K315 Sumoylation
K315 Ubiquitination
T318 Phosphorylation
S323 Phosphorylation
T324 Phosphorylation
K326 Acetylation
K326 Methylation
K326 Sumoylation
K326 Ubiquitination
K328 Acetylation
K328 Sumoylation
K328 Ubiquitination
K336 Sumoylation
S348 Phosphorylation
K359 Ubiquitination
Y362 Phosphorylation
S365 Phosphorylation
S368 Phosphorylation
K373 Ubiquitination
C374 S-Nitrosylation
IMPORTANT: For western blots, incubate membrane with diluted antibody in 5% w/v milk , 1X TBS, 0.1% Tween®20 at 4°C with gentle shaking, overnight.

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