CENPM Antibody - #DF2314

(1)   (2)  
Product: CENPM Antibody
Catalog: DF2314
Description: Rabbit polyclonal antibody to CENPM
Application: WB IHC
Reactivity: Human, Mouse
Prediction: Pig, Bovine, Sheep, Dog
Mol.Wt.: 20kDa; 20kD(Calculated).
Uniprot: Q9NSP4
RRID: AB_2839538

View similar products>>

   Size Price Inventory
 100ul $280 In stock
 200ul $350 In stock

Lead Time: Same day delivery

For pricing and ordering contact:
Local distributors
Related Downloads
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse
Prediction:
Pig(100%), Bovine(88%), Sheep(88%), Dog(88%)
Clonality:
Polyclonal
Specificity:
CENPM Antibody detects endogenous levels of total CENPM.
RRID:
AB_2839538
Cite Format: Affinity Biosciences Cat# DF2314, RRID:AB_2839538.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

bK250D10.2; C22orf18; CENP M; Centromere protein M; ICEN 39; ICEN39; Interphase centromere complex protein 39; MGC861; OTTHUMP00000028741; PANE 1; PANE1; Proliferation associated nuclear element 1; Proliferation associated nuclear element protein 1;

Immunogens

Immunogen:
Uniprot:

Q9NSP4(CENPM_HUMAN) >>Visit HPA database.

Gene(ID):
CENPM(79019)
Expression:
Q9NSP4 CENPM_HUMAN:

Isoform 3 is highly expressed in spleen, and intermediately in heart, prostate and ovary. Isoform 3 is highly expressed in resting CD19 B-cells and B-lineage chronic lymphocytic leukemia (B-CLL) cells and weakly expressed in activated B-cells. Isoform 1 is selectively expressed in activated CD19 cells and weakly in resting CD19 B-cells.

Sequence:
MSVLRPLDKLPGLNTATILLVGTEDALLQQLADSMLKEDCASELKVHLAKSLPLPSSVNRPRIDLIVFVVNLHSKYSLQNTEESLRHVDASFFLGKVCFLATGAGRESHCSIHRHTVVKLAHTYQSPLLYCDLEVEGFRATMAQRLVRVLQICAGHVPGVSALNLLSLLRSSEGPSLEDL

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Bovine
88
Sheep
88
Dog
88
Horse
75
Rabbit
67
Xenopus
0
Zebrafish
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q9NSP4 As Substrate

Site PTM Type Enzyme
S2 Phosphorylation
K45 Ubiquitination
S176 Phosphorylation

Research Backgrounds

Function:

Component of the CENPA-NAC (nucleosome-associated) complex, a complex that plays a central role in assembly of kinetochore proteins, mitotic progression and chromosome segregation. The CENPA-NAC complex recruits the CENPA-CAD (nucleosome distal) complex and may be involved in incorporation of newly synthesized CENPA into centromeres.

Subcellular Location:

Nucleus. Cytoplasm. Chromosome>Centromere>Kinetochore.
Note: Nuclear in non-confluent cells and cytoplasmic in confluent or dividing cells (By similarity). Localizes in the kinetochore domain of centromeres.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Isoform 3 is highly expressed in spleen, and intermediately in heart, prostate and ovary. Isoform 3 is highly expressed in resting CD19 B-cells and B-lineage chronic lymphocytic leukemia (B-CLL) cells and weakly expressed in activated B-cells. Isoform 1 is selectively expressed in activated CD19 cells and weakly in resting CD19 B-cells.

Subunit Structure:

Component of the CENPA-NAC complex, at least composed of CENPA, CENPC, CENPH, CENPM, CENPN, CENPT and CENPU. The CENPA-NAC complex interacts with the CENPA-CAD complex, composed of CENPI, CENPK, CENPL, CENPO, CENPP, CENPQ, CENPR and CENPS.

References

1). Upregulation of CENPM facilitates tumor metastasis via the mTOR/p70S6K signaling pathway in pancreatic cancer. ONCOLOGY REPORTS, 2020 (PubMed: 32705259) [IF=3.8]

Application: WB    Species: human    Sample: PANC-1 and CFPAC-1 cells

Figure 2. |Expression level of CENPM in pancreatic cancer cell lines. (A) The relative expression levels of CENPM in pancreatic cancer cell lines were increased compared to the HPNE cell line. *P<0.05, **P<0.001, ***P<0.0005, ****P<0.0001 compared to HPNE. (B and C) The cell morphologies of PANC-1 and CFPAC-1 were altered when CENPM was knocked down by si-RNAs. (D-F) The effect of CENPM-knockdown by si-RNA in PANC-1 and CFPAC-1 cells. The data were obtained from at least three independent experiments. ****P<0.0001 compared to si-NC. CENPM, centromere protein M; si-RNA, small interfering RNA.

Restrictive clause

 

Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

For Research Use Only.
Not for use in diagnostic or therapeutic procedures. Not for resale. Not for distribution without written consent. Affinity Biosciences will not be held responsible for patent infringement or other violations that may occur with the use of our products. Affinity Biosciences, Affinity Biosciences Logo and all other trademarks are the property of Affinity Biosciences LTD.