PARP4 Antibody - #DF2386

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Product: PARP4 Antibody
Catalog: DF2386
Description: Rabbit polyclonal antibody to PARP4
Application: WB IHC IF/ICC
Reactivity: Human, Mouse
Mol.Wt.: 193 kDa; 193kD(Calculated).
Uniprot: Q9UKK3
RRID: AB_2839594

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse
Clonality:
Polyclonal
Specificity:
PARP4 Antibody detects endogenous levels of total PARP4.
RRID:
AB_2839594
Cite Format: Affinity Biosciences Cat# DF2386, RRID:AB_2839594.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

193kDa vault protein; ADP ribosyltransferase (NAD+ poly (ADP ribose) polymerase) like 1; ADP ribosyltransferase like 1; ADP-ribosyltransferase diphtheria toxin-like 4; ADPRT L1; ADPRTL 1; ADPRTL1; ARTD4; H5 proline rich; I alpha I related; KIAA0177; Minor vault p193 protein; OTTHUMP00000042288; p193; PARP 4; PARP related / IalphaI related H5 / proline rich; PARP related; PARPL; PH 5P; PH5P; Poly (ADP ribose) polymerase 4; Poly (ADP ribose) polymerase family member 4; Poly (ADP ribose) synthetase; Poly (ADP ribosyl) transferase like 1; Poly ADP ribose polymerase 4; Poly ADP ribose polymerase family member 4; Vault 3; Vault; Vault poly (ADP ribose) polymerase; Vault poly ADP ribose polymerase; Vault protein 193 kDa; Vault3; von Willebrand factor A domain containing 5C; VPARP; VWA5C;

Immunogens

Immunogen:
Uniprot:

Q9UKK3(PARP4_HUMAN) >>Visit HPA database.

Gene(ID):
PARP4(143)
Expression:
Q9UKK3 PARP4_HUMAN:

Widely expressed; the highest levels are in the kidney; also detected in heart, placenta, lung, liver, skeletal muscle, spleen, leukocytes and pancreas.

Sequence:
MVMGIFANCIFCLKVKYLPQQQKKKLQTDIKENGGKFSFSLNPQCTHIILDNADVLSQYQLNSIQKNHVHIANPDFIWKSIREKRLLDVKNYDPYKPLDITPPPDQKASSSEVKTEGLCPDSATEEEDTVELTEFGMQNVEIPHLPQDFEVAKYNTLEKVGMEGGQEAVVVELQCSRDSRDCPFLISSHFLLDDGMETRRQFAIKKTSEDASEYFENYIEELKKQGFLLREHFTPEATQLASEQLQALLLEEVMNSSTLSQEVSDLVEMIWAEALGHLEHMLLKPVNRISLNDVSKAEGILLLVKAALKNGETAEQLQKMMTEFYRLIPHKGTMPKEVNLGLLAKKADLCQLIRDMVNVCETNLSKPNPPSLAKYRALRCKIEHVEQNTEEFLRVRKEVLQNHHSKSPVDVLQIFRVGRVNETTEFLSKLGNVRPLLHGSPVQNIVGILCRGLLLPKVVEDRGVQRTDVGNLGSGIYFSDSLSTSIKYSHPGETDGTRLLLICDVALGKCMDLHEKDFSLTEAPPGYDSVHGVSQTASVTTDFEDDEFVVYKTNQVKMKYIIKFSMPGDQIKDFHPSDHTELEEYRPEFSNFSKVEDYQLPDAKTSSSTKAGLQDASGNLVPLEDVHIKGRIIDTVAQVIVFQTYTNKSHVPIEAKYIFPLDDKAAVCGFEAFINGKHIVGEIKEKEEAQQEYLEAVTQGHGAYLMSQDAPDVFTVSVGNLPPKAKVLIKITYITELSILGTVGVFFMPATVAPWQQDKALNENLQDTVEKICIKEIGTKQSFSLTMSIEMPYVIEFIFSDTHELKQKRTDCKAVISTMEGSSLDSSGFSLHIGLSAAYLPRMWVEKHPEKESEACMLVFQPDLDVDLPDLASESEVIICLDCSSSMEGVTFLQAKQIALHALSLVGEKQKVNIIQFGTGYKELFSYPKHITSNTMAAEFIMSATPTMGNTDFWKTLRYLSLLYPARGSRNILLVSDGHLQDESLTLQLVKRSRPHTRLFACGIGSTANRHVLRILSQCGAGVFEYFNAKSKHSWRKQIEDQMTRLCSPSCHSVSVKWQQLNPDVPEALQAPAQVPSLFLNDRLLVYGFIPHCTQATLCALIQEKEFRTMVSTTELQKTTGTMIHKLAARALIRDYEDGILHENETSHEMKKQTLKSLIIKLSKENSLITQFTSFVAVEKRDENESPFPDIPKVSELIAKEDVDFLPYMSWQGEPQEAVRNQSLLASSEWPELRLSKRKHRKIPFSKRKMELSQPEVSEDFEEDGLGVLPAFTSNLERGGVEKLLDLSWTESCKPTATEPLFKKVSPWETSTSSFFPILAPAVGSYLPPTARAHSPASLSFASYRQVASFGSAAPPRQFDASQFSQGPVPGTCADWIPQSASCPTGPPQNPPSSPYCGIVFSGSSLSSAQSAPLQHPGGFTTRPSAGTFPELDSPQLHFSLPTDPDPIRGFGSYHPSASSPFHFQPSAASLTANLRLPMASALPEALCSQSRTTPVDLCLLEESVGSLEGSRCPVFAFQSSDTESDELSEVLQDSCFLQIKCDTKDDSILCFLEVKEEDEIVCIQHWQDAVPWTELLSLQTEDGFWKLTPELGLILNLNTNGLHSFLKQKGIQSLGVKGRECLLDLIATMLVLQFIRTRLEKEGIVFKSLMKMDDASISRNIPWAFEAIKQASEWVRRTEGQYPSICPRLELGNDWDSATKQLLGLQPISTVSPLHRVLHYSQG

PTMs - Q9UKK3 As Substrate

Site PTM Type Enzyme
K16 Ubiquitination
Y17 Phosphorylation
K23 Ubiquitination
T28 Phosphorylation
K31 Ubiquitination
S63 Phosphorylation
K79 Ubiquitination
K90 Ubiquitination
Y95 Phosphorylation
K96 Ubiquitination
T101 Phosphorylation
K107 Ubiquitination
K206 Ubiquitination
Y214 Phosphorylation
Y218 Phosphorylation
K223 Ubiquitination
K224 Ubiquitination
K296 Ubiquitination
K305 Ubiquitination
K309 Ubiquitination
K331 Ubiquitination
T333 Phosphorylation
K336 Ubiquitination
K346 Ubiquitination
T362 Phosphorylation
S365 Phosphorylation
S371 Phosphorylation
K397 Ubiquitination
K406 Ubiquitination
K429 Ubiquitination
K457 Ubiquitination
T467 Phosphorylation
K572 Ubiquitination
K594 Ubiquitination
K604 Ubiquitination
K610 Ubiquitination
K629 Ubiquitination
K656 Ubiquitination
K664 Ubiquitination
K677 Ubiquitination
T768 Phosphorylation
K771 Ubiquitination
K775 Ubiquitination
S830 Phosphorylation
S836 Phosphorylation
S904 Phosphorylation
Y959 Phosphorylation
K1030 Ubiquitination
K1037 Ubiquitination
S1048 Phosphorylation
T1109 Phosphorylation
S1112 Phosphorylation
T1120 Phosphorylation
T1122 Phosphorylation
K1126 Ubiquitination
K1151 Ubiquitination
T1173 Phosphorylation
S1174 Phosphorylation
S1186 Phosphorylation
K1193 Ubiquitination
K1200 Ubiquitination
S1223 Phosphorylation
S1227 Phosphorylation
S1228 Phosphorylation
S1236 Phosphorylation
S1253 Phosphorylation
S1288 Phosphorylation
S1292 Phosphorylation
K1294 Ubiquitination
K1303 Ubiquitination
K1304 Ubiquitination
R1332 Methylation
S1335 Phosphorylation
S1338 Phosphorylation
S1340 Phosphorylation
S1343 Phosphorylation
S1349 Phosphorylation
S1434 Phosphorylation
R1449 Methylation
R1476 Methylation
T1493 Phosphorylation
T1494 Phosphorylation
S1504 Phosphorylation
S1507 Phosphorylation
S1535 Phosphorylation
S1548 Phosphorylation
T1600 Phosphorylation
S1605 Phosphorylation
S1614 Phosphorylation
T1629 Phosphorylation
K1648 Ubiquitination
S1649 Phosphorylation
S1710 Phosphorylation
S1713 Phosphorylation
Y1721 Phosphorylation
S1722 Phosphorylation

Research Backgrounds

Function:

Mono-ADP-ribosyltransferase that mediates mono-ADP-ribosylation of target proteins.

Subcellular Location:

Cytoplasm. Nucleus. Cytoplasm>Cytoskeleton>Spindle.
Note: Also found in the nucleus, associated with mitotic spindles.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Widely expressed; the highest levels are in the kidney; also detected in heart, placenta, lung, liver, skeletal muscle, spleen, leukocytes and pancreas.

Subunit Structure:

Component of the vault ribonucleoprotein particle, at least composed of MVP, PARP4 and one or more vault RNAs (vRNAs). Interacts with TEP1.

Research Fields

· Cellular Processes > Cell growth and death > Apoptosis.   (View pathway)

· Cellular Processes > Cell growth and death > Necroptosis.   (View pathway)

· Genetic Information Processing > Replication and repair > Base excision repair.

Restrictive clause

 

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