MIBP1 Antibody - #DF2398

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Product: MIBP1 Antibody
Catalog: DF2398
Description: Rabbit polyclonal antibody to MIBP1
Application: WB
Reactivity: Human, Mouse, Rat
Prediction: Bovine, Horse, Sheep, Rabbit, Chicken
Mol.Wt.: 35,39 kDa; 269kD(Calculated).
Uniprot: P31629 | Q38G99
RRID: AB_2839605

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 100ul $280 In stock
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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Bovine(88%), Horse(88%), Sheep(88%), Rabbit(100%), Chicken(100%)
Clonality:
Polyclonal
Specificity:
MIBP1 Antibody detects endogenous levels of total MIBP1.
RRID:
AB_2839605
Cite Format: Affinity Biosciences Cat# DF2398, RRID:AB_2839605.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

HIV EP2; HIV-EP2; HIVEP2; human immunodeficiency virus type I enhancer binding protein 2; Human immunodeficiency virus type I enhancer-binding protein 2; MBP 2; MBP-2; MHC binding protein 2; MHC-binding protein 2; MIBP1; SHN2; Transcription factor HIVEP2; ZAS2; ZEP2_HUMAN; ZNF40B;

Immunogens

Immunogen:

A synthesized peptide derived from human MIBP1, corresponding to a region within the internal amino acids.

Uniprot:

P31629(ZEP2_HUMAN) >>Visit HPA database.

Gene(ID):
HIVEP2(3097)
Expression:
P31629 ZEP2_HUMAN:

Expressed in brain and skeletal muscle.

Description:
This protein specifically binds to the DNA sequence 5'-GGGACTTTCC-3' which is found in the enhancer elements of numerous viral promoters such as those of SV40, CMV, or HIV1. In addition, related sequences are found in the enhancer elements of a number of cellular promoters, including those of the class I MHC, interleukin-2 receptor, somatostatin receptor II, and interferon-beta genes. It may act in T-cell activation.
Sequence:
MDTGDTALGQKATSRSGETDKASGRWRQEQSAVIKMSTFGSHEGQRQPQIEPEQIGNTASAQLFGSGKLASPSEVVQQVAEKQYPPHRPSPYSCQHSLSFPQHSLPQGVMHSTKPHQSLEGPPWLFPGPLPSVASEDLFPFPIHGHSGGYPRKKISSLNPAYSQYSQKSIEQAEEAHKKEHKPKKPGKYICPYCSRACAKPSVLKKHIRSHTGERPYPCIPCGFSFKTKSNLYKHRKSHAHAIKAGLVPFTESAVSKLDLEAGFIDVEAEIHSDGEQSTDTDEESSLFAEASDKMSPGPPIPLDIASRGGYHGSLEESLGGPMKVPILIIPKSGIPLPNESSQYIGPDMLPNPSLNTKADDSHTVKQKLALRLSEKKGQDSEPSLNLLSPHSKGSTDSGYFSRSESAEQQISPPNTNAKSYEEIIFGKYCRLSPRNALSVTTTSQERAAMGRKGIMEPLPHVNTRLDVKMFEDPVSQLIPSKGDVDPSQTSMLKSTKFNSESRQPQIIPSSIRNEGKLYPANFQGSNPVLLEAPVDSSPLIRSNSVPTSSATNLTIPPSLRGSHSFDERMTGSDDVFYPGTVGIPPQRMLRRQAAFELPSVQEGHVEVEHHGRMLKGISSSSLKEKKLSPGDRVGYDYDVCRKPYKKWEDSETPKQNYRDISCLSSLKHGGEYFMDPVVPLQGVPSMFGTTCENRKRRKEKSVGDEEDTPMICSSIVSTPVGIMASDYDPKLQMQEGVRSGFAMAGHENLSHGHTERFDPCRPQLQPGSPSLVSEESPSAIDSDKMSDLGGRKPPGNVISVIQHTNSLSRPNSFERSESAELVACTQDKAPSPSETCDSEISEAPVSPEWAPPGDGAESGGKPSPSQQVQQQSYHTQPRLVRQHNIQVPEIRVTEEPDKPEKEKEAQSKEPEKPVEEFQWPQRSETLSQLPAEKLPPKKKRLRLADMEHSSGESSFESTGTGLSRSPSQESNLSHSSSFSMSFEREETSKLSALPKQDEFGKHSEFLTVPAGSYSLSVPGHHHQKEMRRCSSEQMPCPHPAEVPEVRSKSFDYGNLSHAPVSGAAASTVSPSRERKKCFLVRQASFSGSPEISQGEVGMDQSVKQEQLEHLHAGLRSGWHHGPPAVLPPLQQEDPGKQVAGPCPPLSSGPLHLAQPQIMHMDSQESLRNPLIQPTSYMTSKHLPEQPHLFPHQETIPFSPIQNALFQFQYPTVCMVHLPAQQPPWWQAHFPHPFAQHPQKSYGKPSFQTEIHSSYPLEHVAEHTGKKPAEYAHTKEQTYPCYSGASGLHPKNLLPKFPSDQSSKSTETPSEQVLQEDFASANAGSLQSLPGTVVPVRIQTHVPSYGSVMYTSISQILGQNSPAIVICKVDENMTQRTLVTNAAMQGIGFNIAQVLGQHAGLEKYPIWKAPQTLPLGLESSIPLCLPSTSDSVATLGGSKRMLSPASSLELFMETKQQKRVKEEKMYGQIVEELSAVELTNSDIKKDLSRPQKPQLVRQGCASEPKDGLQSGSSSFSSLSPSSSQDYPSVSPSSREPFLPSKEMLSGSRAPLPGQKSSGPSESKESSDELDIDETASDMSMSPQSSSLPAGDGQLEEEGKGHKRPVGMLVRMASAPSGNVADSTLLLTDMADFQQILQFPSLRTTTTVSWCFLNYTKPNYVQQATFKSSVYASWCISSCNPNPSGLNTKTTLALLRSKQKITAEIYTLAAMHRPGTGKLTSSSAWKQFTQMKPDASFLFGSKLERKLVGNILKERGKGDIHGDKDIGSKQTEPIRIKIFEGGYKSNEDYVYVRGRGRGKYICEECGIRCKKPSMLKKHIRTHTDVRPYVCKLCNFAFKTKGNLTKHMKSKAHMKKCLELGVSMTSVDDTETEEAENLEDLHKAAEKHSMSSISTDHQFSDAEESDGEDGDDNDDDDEDEDDFDDQGDLTPKTRSRSTSPQPPRFSSLPVNVGAVPHGVPSDSSLGHSSLISYLVTLPSIRVTQLMTPSDSCEDTQMTEYQRLFQSKSTDSEPDKDRLDIPSCMDEECMLPSEPSSSPRDFSPSSHHSSPGYDSSPCRDNSPKRYLIPKGDLSPRRHLSPRRDLSPMRHLSPRKEAALRREMSQRDVSPRRHLSPRRPVSPGKDITARRDLSPRRERRYMTTIRAPSPRRALYHNPPLSMGQYLQAEPIVLGPPNLRRGLPQVPYFSLYGDQEGAYEHPGSSLFPEGPNDYVFSHLPLHSQQQVRAPIPMVPVGGIQMVHSMPPALSSLHPSPTLPLPMEGFEEKKGASGESFSKDPYVLSKQHEKRGPHALQSSGPPSTPSSPRLLMKQSTSEDSLNATEREQEENIQTCTKAIASLRIATEEAALLGPDQPARVQEPHQNPLGSAHVSIRHFSRPEPGQPCTSATHPDLHDGEKDNFGTSQTPLAHSTFYSKSCVDDKQLDFHSSKELSSSTEESKDPSSEKSQLH

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Rabbit
100
Chicken
100
Horse
88
Bovine
88
Sheep
88
Pig
0
Dog
0
Xenopus
0
Zebrafish
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P31629/Q38G99 As Substrate

Site PTM Type Enzyme
S41 Phosphorylation
S71 Phosphorylation
K154 Ubiquitination
S166 Phosphorylation
K168 Ubiquitination
S169 Phosphorylation
K178 Ubiquitination
K179 Acetylation
Y189 Phosphorylation
Y193 Phosphorylation
S195 Phosphorylation
T212 Phosphorylation
S296 Phosphorylation
S314 Phosphorylation
K332 Ubiquitination
K358 Ubiquitination
K366 Ubiquitination
K368 Ubiquitination
S374 Phosphorylation
S389 Phosphorylation
S402 Phosphorylation
S406 Phosphorylation
S412 Phosphorylation
S420 Phosphorylation
Y421 Phosphorylation
K428 Ubiquitination
S433 Phosphorylation
K453 Ubiquitination
K469 Ubiquitination
K482 Ubiquitination
K517 Ubiquitination
S526 Phosphorylation
T548 Phosphorylation
T552 Phosphorylation
S565 Phosphorylation
K616 Methylation
S619 Phosphorylation
S622 Phosphorylation
K624 Methylation
K626 Methylation
S629 Phosphorylation
Y638 Phosphorylation
Y645 Phosphorylation
K655 Ubiquitination
S702 Phosphorylation
S769 Phosphorylation
S771 Phosphorylation
S777 Phosphorylation
S807 Phosphorylation
S813 Phosphorylation
S819 Phosphorylation
T926 Phosphorylation
S950 Phosphorylation
S951 Phosphorylation
S955 Phosphorylation
S964 Phosphorylation
S968 Phosphorylation
S1031 Phosphorylation
S1032 Phosphorylation
S1048 Phosphorylation
S1050 Phosphorylation
S1085 Phosphorylation
S1087 Phosphorylation
K1291 Ubiquitination
K1296 Ubiquitination
S1299 Phosphorylation
S1443 Phosphorylation
S1446 Phosphorylation
S1447 Phosphorylation
T1454 Phosphorylation
K1455 Ubiquitination
S1579 Phosphorylation
S1586 Phosphorylation
K1666 Methylation
K1717 Ubiquitination
K1725 Ubiquitination
K1731 Ubiquitination
K1741 Ubiquitination
K1745 Ubiquitination
K1752 Ubiquitination
K1768 Ubiquitination
Y1782 Phosphorylation
K1783 Ubiquitination
S1784 Phosphorylation
Y1788 Phosphorylation
K1798 Ubiquitination
T1820 Phosphorylation
T1822 Phosphorylation
T1936 Phosphorylation
S1937 Phosphorylation
T1981 Phosphorylation
T1985 Phosphorylation
S1987 Phosphorylation
T1996 Phosphorylation
Y1998 Phosphorylation
S2034 Phosphorylation
S2035 Phosphorylation
S2059 Phosphorylation
S2071 Phosphorylation
S2077 Phosphorylation
S2083 Phosphorylation
K2092 Ubiquitination
S2112 Phosphorylation
S2118 Phosphorylation
S2130 Phosphorylation
S2297 Phosphorylation
T2298 Phosphorylation
S2300 Phosphorylation
S2301 Phosphorylation
K2331 Acetylation
T2402 Phosphorylation
S2435 Phosphorylation
S2440 Phosphorylation
S2443 Phosphorylation

Research Backgrounds

Function:

This protein specifically binds to the DNA sequence 5'-GGGACTTTCC-3' which is found in the enhancer elements of numerous viral promoters such as those of SV40, CMV, or HIV1. In addition, related sequences are found in the enhancer elements of a number of cellular promoters, including those of the class I MHC, interleukin-2 receptor, somatostatin receptor II, and interferon-beta genes. It may act in T-cell activation.

Subcellular Location:

Nucleus.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Expressed in brain and skeletal muscle.

Subunit Structure:

Interacts with TCF4.

Restrictive clause

 

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For Research Use Only.
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