Steroidogenic Factor 1 Antibody | Affinity Biosciences

1/1

Western blot analysis of extracts from Rat brain, using Steroidogenic Factor 1 Antibody at 1/1000 dilution.

Product:	Steroidogenic Factor 1 Antibody
Catalog:	DF2406
Description:	Rabbit polyclonal antibody to Steroidogenic Factor 1
Application:	WB
Reactivity:	Human, Mouse, Rat
Prediction:	Pig, Bovine, Horse, Sheep, Rabbit, Dog
Mol.Wt.:	55 kDa; 52kD(Calculated).
Uniprot:	Q13285
RRID:	AB_2839613

View similar products>>

Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$280	In stock
200ul	$350	In stock

Lead Time: Same day delivery

For pricing and ordering contact:
Local distributors

Related Downloads

MS Report

HPLC Report

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:500-1:2000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse,Rat

Prediction:

Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%)

Clonality:

Polyclonal

Specificity:

Steroidogenic Factor 1 Antibody detects endogenous levels of total Steroidogenic Factor 1.

RRID:

AB_2839613
Cite Format: Affinity Biosciences Cat# DF2406, RRID:AB_2839613.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

AD4BP; Adrenal 4 binding protein; Adrenal 4-binding protein; ELP; FTZ 1; FTZ1; FTZF 1; FTZF1; Fushi tarazu factor (Drosophila) homolog 1; Fushi tarazu factor homolog 1; hSF 1; NR5A1; Nuclear receptor AdBP4; Nuclear receptor subfamily 5 group A member 1; POF7; SF 1; SF-1; SF1; SPGF8; SRXY3; Steroid hormone receptor Ad4BP; Steroidogenic factor 1; Steroidogenic factor 1 nuclear receptor; STF 1; STF-1; STF1; STF1_HUMAN;

Immunogens

Immunogen:

Uniprot:

Q13285(STF1_HUMAN) >>Visit HPA database.

Gene(ID):

NR5A1(2516)

Expression:

Q13285 STF1_HUMAN:

High expressed in the adrenal cortex, the ovary, the testis, and the spleen (PubMed:9177385).

Description:

vTranscriptional activator. Seems to be essential for sexual differentiation and formation of the primary steroidogenic tissues. Binds to the Ad4 site found in the promoter region of steroidogenic P450 genes such as CYP11A, CYP11B and CYP21B. Also regulates the AMH/Muellerian inhibiting substance gene as well as the AHCH and STAR genes. 5'-YCAAGGYC-3' and 5'-RRAGGTCA-3' are the consensus sequences for the recognition by NR5A1. The SFPQ-NONO-NR5A1 complex binds to the CYP17 promoter and regulates basal and cAMP-dependent transcriptional avtivity. Binds phosphatidylcholine (By similarity). Binds phospholipids with a phosphatidylinositol (PI) headgroup, in particular PI(3,4)P2 and PI(3,4,5)P3.

Sequence:

Q13285 STF1_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MDYSYDEDLDELCPVCGDKVSGYHYGLLTCESCKGFFKRTVQNNKHYTCTESQSCKIDKTQRKRCPFCRFQKCLTVGMRLEAVRADRMRGGRNKFGPMYKRDRALKQQKKAQIRANGFKLETGPPMGVPPPPPPAPDYVLPPSLHGPEPKGLAAGPPAGPLGDFGAPALPMAVPGAHGPLAGYLYPAFPGRAIKSEYPEPYASPPQPGLPYGYPEPFSGGPNVPELILQLLQLEPDEDQVRARILGCLQEPTKSRPDQPAAFGLLCRMADQTFISIVDWARRCMVFKELEVADQMTLLQNCWSELLVFDHIYRQVQHGKEGSILLVTGQEVELTTVATQAGSLLHSLVLRAQELVLQLLALQLDRQEFVCLKFIILFSLDLKFLNNHILVKDAQEKANAALLDYTLCHYPHCGDKFQQLLLCLVEVRALSMQAKEYLYHKHLGNEMPRNNLLIEMLQAKQT

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Pig

100

Horse

100

Bovine

100

Sheep

100

Dog

100

Rabbit

100

Xenopus

Zebrafish

Chicken

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q13285 As Substrate

Q13285

Site	PTM Type	Enzyme	Source
K34	Acetylation		Uniprot
K38	Acetylation		Uniprot
K72	Acetylation		Uniprot
K100	Methylation		Uniprot
K106	Acetylation	PR:000007102 (EP300)	Uniprot
K109	Acetylation	PR:000007102 (EP300)	Uniprot
K110	Acetylation	PR:000007102 (EP300)	Uniprot
K119	Sumoylation		Uniprot
K119	Ubiquitination		Uniprot
K194	Sumoylation		Uniprot
S203	Phosphorylation	P28482 (MAPK1) , P50613 (CDK7)	Uniprot
S430	Phosphorylation		Uniprot

Research Backgrounds

Q13285_STF1_HUMAN

Function:

Transcriptional activator. Essential for sexual differentiation and formation of the primary steroidogenic tissues. Binds to the Ad4 site found in the promoter region of steroidogenic P450 genes such as CYP11A, CYP11B and CYP21B. Also regulates the AMH/Muellerian inhibiting substance gene as well as the AHCH and STAR genes. 5'-YCAAGGYC-3' and 5'-RRAGGTCA-3' are the consensus sequences for the recognition by NR5A1. The SFPQ-NONO-NR5A1 complex binds to the CYP17 promoter and regulates basal and cAMP-dependent transcriptional activity. Binds phosphatidylcholine (By similarity). Binds phospholipids with a phosphatidylinositol (PI) headgroup, in particular PI(3,4)P2 and PI(3,4,5)P3. Activated by the phosphorylation of NR5A1 by HIPK3 leading to increased steroidogenic gene expression upon cAMP signaling pathway stimulation.

PTMs:

Acetylation stimulates the transcriptional activity.

Sumoylation reduces CDK7-mediated phosphorylation on Ser-203.

Phosphorylated on Ser-203 by CDK7. This phosphorylation promotes transcriptional activity.

Subcellular Location:

Nucleus.

Tissue Specificity:

High expressed in the adrenal cortex, the ovary, the testis, and the spleen.

Subunit Structure:

Binds DNA as a monomer. Interacts with NR0B2 and PPARGC1A (By similarity). Part of a complex consisting of SFPQ, NONO and NR5A1. Interacts with NCOA2. Interacts with DGKQ and CDK7. Binds to and activated by HIPK3.

Family&Domains:

Belongs to the nuclear hormone receptor family. NR5 subfamily.

References

1). The miR-34b/MEK/ERK pathway is regulated by NR5A1 and promotes differentiation in primary bovine Sertoli cells. Theriogenology (PubMed: 38100994) [IF=2.8]

Restrictive clause

Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

For Research Use Only.
Not for use in diagnostic or therapeutic procedures. Not for resale. Not for distribution without written consent. Affinity Biosciences will not be held responsible for patent infringement or other violations that may occur with the use of our products. Affinity Biosciences, Affinity Biosciences Logo and all other trademarks are the property of Affinity Biosciences LTD.

Steroidogenic Factor 1 Antibody - #DF2406