JMJD1B Antibody - #DF2417

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Product: JMJD1B Antibody
Catalog: DF2417
Description: Rabbit polyclonal antibody to JMJD1B
Application: WB IF/ICC
Reactivity: Human, Mouse
Prediction: Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken
Mol.Wt.: 240 kDa; 192kD(Calculated).
Uniprot: Q7LBC6
RRID: AB_2839623

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 100ul $280 In stock
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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse
Prediction:
Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(83%)
Clonality:
Polyclonal
Specificity:
JMJD1B Antibody detects endogenous levels of total JMJD1B.
RRID:
AB_2839623
Cite Format: Affinity Biosciences Cat# DF2417, RRID:AB_2839623.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

5qNCA; C5orf7; JHDM2B; JmjC domain containing histone demethylation protein 2B; JmjC domain-containing histone demethylation protein 2B; jmjd1b; Jumonji domain containing 1B; Jumonji domain containing protein 1B; Jumonji domain-containing protein 1B; KDM3B; KDM3B_HUMAN; KIAA1082; Lysine (K) specific demethylase 3B; Lysine-specific demethylase 3B; NET22; Nuclear protein 5qNCA;

Immunogens

Immunogen:
Uniprot:

Q7LBC6(KDM3B_HUMAN) >>Visit HPA database.

Gene(ID):
KDM3B(51780)
Expression:
Q7LBC6 KDM3B_HUMAN:

Ubiquitous. Highly expressed in placenta, skeletal muscle, kidney, heart and liver.

Description:
Histone demethylase that specifically demethylates 'Lys-9' of histone H3, thereby playing a central role in histone code. Demethylation of Lys residue generates formaldehyde and succinate. May have tumor suppressor activity.
Sequence:
MADAAASPVGKRLLLLFADTAASASASAPAAAAASGDPGPALRTRAWRAGTVRAMSGAVPQDLAIFVEFDGCNWKQHSWVKVHAEEVIVLLLEGSLVWAPREDPVLLQGIRVSIAQWPALTFTPLVDKLGLGSVVPVEYLLDRELRFLSDANGLHLFQMGTDSQNQILLEHAALRETVNALISDQKLQEIFSRGPYSVQGHRVKIYQPEGEEGWLYGVVSHQDSITRLMEVSVTESGEIKSVDPRLIHVMLMDNSAPQSEGGTLKAVKSSKGKKKRESIEGKDGRRRKSASDSGCDPASKKLKGDRGEVDSNGSDGGEASRGPWKGGNASGEPGLDQRAKQPPSTFVPQINRNIRFATYTKENGRTLVVQDEPVGGDTPASFTPYSTATGQTPLAPEVGGAENKEAGKTLEQVGQGIVASAAVVTTASSTPNTVRISDTGLAAGTVPEKQKGSRSQASGENSRNSILASSGFGAPLPSSSQPLTFGSGRSQSNGVLATENKPLGFSFGCSSAQEAQKDTDLSKNLFFQCMSQTLPTSNYFTTVSESLADDSSSRDSFKQSLESLSSGLCKGRSVLGTDTKPGSKAGSSVDRKVPAESMPTLTPAFPRSLLNARTPENHENLFLQPPKLSREEPSNPFLAFVEKVEHSPFSSFASQASGSSSSATTVTSKVAPSWPESHSSADSASLAKKKPLFITTDSSKLVSGVLGSALTSGGPSLSAMGNGRSSSPTSSLTQPIEMPTLSSSPTEERPTVGPGQQDNPLLKTFSNVFGRHSGGFLSSPADFSQENKAPFEAVKRFSLDERSLACRQDSDSSTNSDLSDLSDSEEQLQAKTGLKGIPEHLMGKLGPNGERSAELLLGKSKGKQAPKGRPRTAPLKVGQSVLKDVSKVKKLKQSGEPFLQDGSCINVAPHLHKCRECRLERYRKFKEQEQDDSTVACRFFHFRRLIFTRKGVLRVEGFLSPQQSDPDAMNLWIPSSSLAEGIDLETSKYILANVGDQFCQLVMSEKEAMMMVEPHQKVAWKRAVRGVREMCDVCETTLFNIHWVCRKCGFGVCLDCYRLRKSRPRSETEEMGDEEVFSWLKCAKGQSHEPENLMPTQIIPGTALYNIGDMVHAARGKWGIKANCPCISRQNKSVLRPAVTNGMSQLPSINPSASSGNETTFSGGGGPAPVTTPEPDHVPKADSTDIRSEEPLKTDSSASNSNSELKAIRPPCPDTAPPSSALHWLADLATQKAKEETKEAGSLRSVLNKESHSPFGLDSFNSTAKVSPLTPKLFNSLLLGPTASNNKTEGSSLRDLLHSGPGKLPQTPLDTGIPFPPVFSTSSAGVKSKASLPNFLDHIIASVVENKKTSDASKRACNLTDTQKEVKEMVMGLNVLDPHTSHSWLCDGRLLCLHDPSNKNNWKIFRECWKQGQPVLVSGVHKKLKSELWKPEAFSQEFGDQDVDLVNCRNCAIISDVKVRDFWDGFEIICKRLRSEDGQPMVLKLKDWPPGEDFRDMMPTRFEDLMENLPLPEYTKRDGRLNLASRLPSYFVRPDLGPKMYNAYGLITAEDRRVGTTNLHLDVSDAVNVMVYVGIPIGEGAHDEEVLKTIDEGDADEVTKQRIHDGKEKPGALWHIYAAKDAEKIRELLRKVGEEQGQENPPDHDPIHDQSWYLDQTLRKRLYEEYGVQGWAIVQFLGDAVFIPAGAPHQVHNLYSCIKVAEDFVSPEHVKHCFRLTQEFRHLSNTHTNHEDKLQVKNIIYHAVKDAVGTLKAHESKLARS

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Dog
100
Rabbit
100
Chicken
83
Xenopus
78
Zebrafish
73
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q7LBC6 As Substrate

Site PTM Type Enzyme
A2 Acetylation
S7 Phosphorylation
K11 Ubiquitination
R143 Methylation
K186 Ubiquitination
K204 Ubiquitination
T263 Phosphorylation
S278 Phosphorylation
S289 Phosphorylation
S291 Phosphorylation
S299 Phosphorylation
K300 Acetylation
S311 Phosphorylation
S314 Phosphorylation
S320 Phosphorylation
K325 Ubiquitination
R355 Methylation
T358 Phosphorylation
K361 Acetylation
T378 Phosphorylation
T383 Phosphorylation
S437 Phosphorylation
T439 Phosphorylation
S480 Phosphorylation
T484 Phosphorylation
S487 Phosphorylation
S492 Phosphorylation
K501 Acetylation
S506 Phosphorylation
S551 Phosphorylation
S552 Phosphorylation
S553 Phosphorylation
S556 Phosphorylation
K558 Ubiquitination
S560 Phosphorylation
K570 Ubiquitination
S573 Phosphorylation
T579 Phosphorylation
K580 Ubiquitination
S583 Phosphorylation
K584 Ubiquitination
S588 Phosphorylation
K592 Sumoylation
K592 Ubiquitination
T602 Phosphorylation
S608 Phosphorylation
T614 Phosphorylation
S647 Phosphorylation
S654 Phosphorylation
K688 Ubiquitination
S703 Phosphorylation
S708 Phosphorylation
T711 Phosphorylation
S725 Phosphorylation
S726 Phosphorylation
S727 Phosphorylation
T729 Phosphorylation
S730 Phosphorylation
S731 Phosphorylation
T733 Phosphorylation
T740 Phosphorylation
S742 Phosphorylation
S743 Phosphorylation
S744 Phosphorylation
T751 Phosphorylation
S766 Phosphorylation
R771 Methylation
S773 Phosphorylation
S778 Phosphorylation
S779 Phosphorylation
S784 Phosphorylation
K788 Sumoylation
K788 Ubiquitination
K795 Acetylation
K795 Ubiquitination
S798 Phosphorylation
K835 Ubiquitination
K844 Ubiquitination
K859 Ubiquitination
K876 Ubiquitination
K883 Ubiquitination
K892 Ubiquitination
K913 Ubiquitination
K924 Ubiquitination
K926 Methylation
K926 Ubiquitination
K1121 Ubiquitination
T1171 Phosphorylation
T1172 Phosphorylation
K1193 Ubiquitination
T1194 Phosphorylation
S1196 Phosphorylation
S1197 Phosphorylation
S1199 Phosphorylation
S1203 Phosphorylation
K1206 Ubiquitination
K1232 Ubiquitination
S1245 Phosphorylation
K1249 Ubiquitination
S1251 Phosphorylation
S1253 Phosphorylation
S1259 Phosphorylation
S1262 Phosphorylation
T1263 Phosphorylation
K1265 Ubiquitination
S1267 Phosphorylation
T1270 Phosphorylation
K1272 Ubiquitination
S1276 Phosphorylation
S1284 Phosphorylation
K1287 Ubiquitination
T1288 Phosphorylation
S1299 Phosphorylation
K1303 Ubiquitination
T1307 Phosphorylation
T1311 Phosphorylation
K1327 Ubiquitination
K1329 Ubiquitination
S1331 Phosphorylation
K1347 Ubiquitination
K1354 Ubiquitination
K1364 Ubiquitination
K1399 Ubiquitination
K1410 Ubiquitination
K1422 Ubiquitination
K1425 Ubiquitination
K1430 Ubiquitination
K1458 Ubiquitination
K1471 Ubiquitination
K1484 Ubiquitination
K1486 Ubiquitination
K1516 Ubiquitination
Y1541 Phosphorylation
K1600 Ubiquitination
K1620 Ubiquitination
K1631 Ubiquitination
S1706 Phosphorylation
K1711 Ubiquitination
K1733 Ubiquitination
K1752 Methylation
K1752 Ubiquitination
K1757 Ubiquitination

Research Backgrounds

Function:

Histone demethylase that specifically demethylates 'Lys-9' of histone H3, thereby playing a central role in histone code. Demethylation of Lys residue generates formaldehyde and succinate. May have tumor suppressor activity.

Subcellular Location:

Nucleus.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Ubiquitous. Highly expressed in placenta, skeletal muscle, kidney, heart and liver.

Family&Domains:

Leu-Xaa-Xaa-Leu-Leu (LXXLL) motifs are known to mediate the association with nuclear receptors.

Belongs to the JHDM2 histone demethylase family.

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