Cytochrome P450 reductase Antibody - #DF2613

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Product: Cytochrome P450 reductase Antibody
Catalog: DF2613
Description: Rabbit polyclonal antibody to Cytochrome P450 reductase
Application: WB
Reactivity: Human, Mouse, Rat
Prediction: Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus
Mol.Wt.: 78 kDa; 77kD(Calculated).
Uniprot: P16435
RRID: AB_2839819

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 100ul $280 In stock
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Related Downloads
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(88%), Bovine(100%), Horse(100%), Sheep(88%), Rabbit(100%), Dog(100%), Chicken(100%), Xenopus(100%)
Clonality:
Polyclonal
Specificity:
Cytochrome P450 reductase Antibody detects endogenous levels of total Cytochrome P450 reductase.
RRID:
AB_2839819
Cite Format: Affinity Biosciences Cat# DF2613, RRID:AB_2839819.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

CPR; CYPOR; DKFZp686G04235; FLJ26468; NADPH Cytochrome P450 Reductase; NADPH dependent cytochrome P450 reductase; NADPH--cytochrome P450 reductase; NCPR_HUMAN; P450 (cytochrome) oxidoreductase; P450 Cytochrome Oxidoreductase; P450R; por;

Immunogens

Immunogen:
Uniprot:

P16435(NCPR_HUMAN) >>Visit HPA database.

Gene(ID):
POR(5447)
Description:
This enzyme is required for electron transfer from NADP to cytochrome P450 in microsomes. It can also provide electron transfer to heme oxygenase and cytochrome B5.
Sequence:
MGDSHVDTSSTVSEAVAEEVSLFSMTDMILFSLIVGLLTYWFLFRKKKEEVPEFTKIQTLTSSVRESSFVEKMKKTGRNIIVFYGSQTGTAEEFANRLSKDAHRYGMRGMSADPEEYDLADLSSLPEIDNALVVFCMATYGEGDPTDNAQDFYDWLQETDVDLSGVKFAVFGLGNKTYEHFNAMGKYVDKRLEQLGAQRIFELGLGDDDGNLEEDFITWREQFWPAVCEHFGVEATGEESSIRQYELVVHTDIDAAKVYMGEMGRLKSYENQKPPFDAKNPFLAAVTTNRKLNQGTERHLMHLELDISDSKIRYESGDHVAVYPANDSALVNQLGKILGADLDVVMSLNNLDEESNKKHPFPCPTSYRTALTYYLDITNPPRTNVLYELAQYASEPSEQELLRKMASSSGEGKELYLSWVVEARRHILAILQDCPSLRPPIDHLCELLPRLQARYYSIASSSKVHPNSVHICAVVVEYETKAGRINKGVATNWLRAKEPAGENGGRALVPMFVRKSQFRLPFKATTPVIMVGPGTGVAPFIGFIQERAWLRQQGKEVGETLLYYGCRRSDEDYLYREELAQFHRDGALTQLNVAFSREQSHKVYVQHLLKQDREHLWKLIEGGAHIYVCGDARNMARDVQNTFYDIVAELGAMEHAQAVDYIKKLMTKGRYSLDVWS

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Horse
100
Bovine
100
Dog
100
Xenopus
100
Chicken
100
Rabbit
100
Pig
88
Sheep
88
Zebrafish
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P16435 As Substrate

Site PTM Type Enzyme
G2 Acetylation
K56 Ubiquitination
T59 Phosphorylation
S62 Phosphorylation
S63 Phosphorylation
S68 Phosphorylation
K72 Acetylation
K186 Ubiquitination
Y245 Phosphorylation
T251 Phosphorylation
Y259 Phosphorylation
K267 Methylation
K267 Ubiquitination
K273 Ubiquitination
T296 Phosphorylation
K311 Ubiquitination
Y373 Phosphorylation
Y374 Phosphorylation
Y416 Phosphorylation
K487 Ubiquitination
K523 Ubiquitination
T525 Phosphorylation
T526 Phosphorylation
Y564 Phosphorylation
Y573 Phosphorylation
Y575 Phosphorylation
Y604 Phosphorylation
K610 Ubiquitination

Research Backgrounds

Function:

This enzyme is required for electron transfer from NADP to cytochrome P450 in microsomes. It can also provide electron transfer to heme oxygenase and cytochrome B5.

Subcellular Location:

Endoplasmic reticulum membrane>Single-pass membrane protein>Cytoplasmic side.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Family&Domains:

Belongs to the NADPH--cytochrome P450 reductase family.

In the N-terminal section; belongs to the flavodoxin family.

In the C-terminal section; belongs to the flavoprotein pyridine nucleotide cytochrome reductase family.

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