ING4 Antibody - #DF2635

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Product: ING4 Antibody
Catalog: DF2635
Description: Rabbit polyclonal antibody to ING4
Application: WB IF/ICC
Reactivity: Human, Mouse, Rat
Prediction: Bovine, Horse, Sheep, Rabbit, Dog, Chicken
Mol.Wt.: 29 kDa; 29kD(Calculated).
Uniprot: Q9UNL4
RRID: AB_2839841

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 100ul $280 In stock
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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Bovine(100%), Horse(100%), Sheep(100%), Rabbit(92%), Dog(100%), Chicken(100%)
Clonality:
Polyclonal
Specificity:
ING4 Antibody detects endogenous levels of total ING4.
RRID:
AB_2839841
Cite Format: Affinity Biosciences Cat# DF2635, RRID:AB_2839841.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

Brain my036 protein; Candidate tumor suppressor p33 ING 1 homolog; Candidate tumor suppressor p33 ING1 homolog; D6Wsu147e; D6Xrf92; ING 1 like protein; ING 4; ING1 like protein; ING4; ING4_HUMAN; Inhibitor of growth family member 4; Inhibitor of growth family member 4 long isoform; Inhibitor of growth protein 4; MGC12557; my036; p29 ING 4; p29 ING4; p29ING4;

Immunogens

Immunogen:
Uniprot:

Q9UNL4(ING4_HUMAN) >>Visit HPA database.

Gene(ID):
ING4(51147)
Description:
Component of the HBO1 complex which has a histone H4-specific acetyltransferase activity, a reduced activity toward histone H3 and is responsible for the bulk of histone H4 acetylation in vivo. Through chromatin acetylation it may function in DNA replication. May inhibit tumor progression by modulating the transcriptional output of signaling pathways which regulate cell proliferation. Can suppress brain tumor angiogenesis through transcriptional repression of RELA/NFKB3 target genes when complexed with RELA. May also specifically suppress loss of contact inhibition elicited by activated oncogenes such as MYC. Represses hypoxia inducible factor's (HIF) activity by interacting with HIF prolyl hydroxylase 2 (EGLN1).
Sequence:
MAAGMYLEHYLDSIENLPFELQRNFQLMRDLDQRTEDLKAEIDKLATEYMSSARSLSSEEKLALLKQIQEAYGKCKEFGDDKVQLAMQTYEMVDKHIRRLDTDLARFEADLKEKQIESSDYDSSSSKGKKKGRTQKEKKAARARSKGKNSDEEAPKTAQKKLKLVRTSPEYGMPSVTFGSVHPSDVLDMPVDPNEPTYCLCHQVSYGEMIGCDNPDCSIEWFHFACVGLTTKPRGKWFCPRCSQERKKK

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Horse
100
Bovine
100
Sheep
100
Dog
100
Chicken
100
Rabbit
92
Zebrafish
64
Pig
0
Xenopus
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q9UNL4 As Substrate

Site PTM Type Enzyme
K44 Ubiquitination
T47 Phosphorylation
Y49 Phosphorylation
S51 Phosphorylation
K61 Ubiquitination
K66 Ubiquitination
K74 Ubiquitination
K95 Ubiquitination
K112 Acetylation
K114 Acetylation
S123 Phosphorylation
K127 Acetylation
K129 Acetylation
K130 Acetylation
K131 Acetylation
K136 Acetylation
K139 Acetylation
K146 Acetylation
K148 Acetylation
S150 Phosphorylation
K156 Acetylation
K160 Acetylation
K161 Acetylation
K236 Acetylation

Research Backgrounds

Function:

Component of the HBO1 complex which has a histone H4-specific acetyltransferase activity, a reduced activity toward histone H3 and is responsible for the bulk of histone H4 acetylation in vivo. Through chromatin acetylation it may function in DNA replication. May inhibit tumor progression by modulating the transcriptional output of signaling pathways which regulate cell proliferation. Can suppress brain tumor angiogenesis through transcriptional repression of RELA/NFKB3 target genes when complexed with RELA. May also specifically suppress loss of contact inhibition elicited by activated oncogenes such as MYC. Represses hypoxia inducible factor's (HIF) activity by interacting with HIF prolyl hydroxylase 2 (EGLN1). Can enhance apoptosis induced by serum starvation in mammary epithelial cell line HC11 (By similarity).

PTMs:

Citrullination by PADI4 within the nuclear localization signal disrupts the interaction with p53 and increases susceptibility to degradation.

Subcellular Location:

Nucleus.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Subunit Structure:

Homodimer. Interacts with H3K4me3 and to a lesser extent with H3K4me2, the interaction augments HBO1 acetylation activity on H3 tails. Component of the HBO1 complex composed at least of ING4 or ING5, KAT7/HBO1, MEAF6, and one of JADE1, JADE2 and JADE3. Interacts with EP300, RELA and TP53; these interactions may be indirect. Interacts with EGLN1. Interacts with BCL2A1 (By similarity).

Family&Domains:

The PHD-type zinc finger mediates the binding to H3K4me3.

The N-terminal coiled-coil domain mediates homodimerization.

Belongs to the ING family.

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