CLK3 Antibody - #DF2662

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Product: CLK3 Antibody
Catalog: DF2662
Description: Rabbit polyclonal antibody to CLK3
Application: WB
Reactivity: Human, Mouse, Rat
Prediction: Pig, Bovine, Horse, Sheep, Rabbit, Dog, Xenopus
Mol.Wt.: 59 kDa; 74kD(Calculated).
Uniprot: P49761
RRID: AB_2839868

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 100ul $280 In stock
 200ul $350 In stock

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Xenopus(80%)
Clonality:
Polyclonal
Specificity:
CLK3 Antibody detects endogenous levels of total CLK3.
RRID:
AB_2839868
Cite Format: Affinity Biosciences Cat# DF2662, RRID:AB_2839868.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

CDC like kinase 3; CDC-like kinase 3; CLK3; CLK3_HUMAN; Dual specificity protein kinase CLK3; PHCLK3; PHCLK3/152;

Immunogens

Immunogen:
Uniprot:

P49761(CLK3_HUMAN) >>Visit HPA database.

Gene(ID):
CLK3(1198)
Expression:
P49761 CLK3_HUMAN:

Endothelial cells.

Description:
Phosphorylates serine- and arginine-rich (SR) proteins of the spliceosomal complex. May be a constituent of a network of regulatory mechanisms that enable SR proteins to control RNA splicing. Phosphorylates serines, threonines and tyrosines.
Sequence:
MPVLSARRRELADHAGSGRRSGPSPTARSGPHLSALRAQPARAAHLSGRGTYVRRDTAGGGPGQARPLGPPGTSLLGRGARRSGEGWCPGAFESGARAARPPSRVEPRLATAASREGAGLPRAEVAAGSGRGARSGEWGLAAAGAWETMHHCKRYRSPEPDPYLSYRWKRRRSYSREHEGRLRYPSRREPPPRRSRSRSHDRLPYQRRYRERRDSDTYRCEERSPSFGEDYYGPSRSRHRRRSRERGPYRTRKHAHHCHKRRTRSCSSASSRSQQSSKRSSRSVEDDKEGHLVCRIGDWLQERYEIVGNLGEGTFGKVVECLDHARGKSQVALKIIRNVGKYREAARLEINVLKKIKEKDKENKFLCVLMSDWFNFHGHMCIAFELLGKNTFEFLKENNFQPYPLPHVRHMAYQLCHALRFLHENQLTHTDLKPENILFVNSEFETLYNEHKSCEEKSVKNTSIRVADFGSATFDHEHHTTIVATRHYRPPEVILELGWAQPCDVWSIGCILFEYYRGFTLFQTHENREHLVMMEKILGPIPSHMIHRTRKQKYFYKGGLVWDENSSDGRYVKENCKPLKSYMLQDSLEHVQLFDLMRRMLEFDPAQRITLAEALLHPFFAGLTPEERSFHTSRNPSR

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Dog
100
Rabbit
100
Xenopus
80
Zebrafish
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P49761 As Substrate

Site PTM Type Enzyme
Y155 Phosphorylation
S157 Phosphorylation O14757 (CHEK1)
Y163 Phosphorylation
S165 Phosphorylation
Y166 Phosphorylation
S173 Phosphorylation
Y174 Phosphorylation
S175 Phosphorylation
S197 Phosphorylation
S199 Phosphorylation
S215 Phosphorylation
T217 Phosphorylation
Y218 Phosphorylation
S224 Phosphorylation
S226 Phosphorylation
Y232 Phosphorylation
S265 Phosphorylation
S270 Phosphorylation
S271 Phosphorylation
S280 Phosphorylation
S281 Phosphorylation
S283 Phosphorylation
K288 Ubiquitination
T314 Phosphorylation
K317 Ubiquitination
K328 Sumoylation
K328 Ubiquitination
K334 Ubiquitination
K396 Ubiquitination
K460 Ubiquitination
K553 Ubiquitination
K557 Ubiquitination
K573 Ubiquitination
K577 Ubiquitination
K580 Ubiquitination
S637 Phosphorylation

Research Backgrounds

Function:

Dual specificity kinase acting on both serine/threonine and tyrosine-containing substrates. Phosphorylates serine- and arginine-rich (SR) proteins of the spliceosomal complex. May be a constituent of a network of regulatory mechanisms that enable SR proteins to control RNA splicing and can cause redistribution of SR proteins from speckles to a diffuse nucleoplasmic distribution. Phosphorylates SRSF1 and SRSF3. Regulates the alternative splicing of tissue factor (F3) pre-mRNA in endothelial cells.

PTMs:

Autophosphorylates on all three types of residues.

Subcellular Location:

Nucleus. Cytoplasm. Cytoplasmic vesicle>Secretory vesicle>Acrosome.

Nucleus speckle.
Note: Co-localizes with serine- and arginine-rich (SR) proteins in the nuclear speckles.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Endothelial cells.

Family&Domains:

Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. Lammer subfamily.

Restrictive clause

 

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