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  • Product Name
    IRAK4 Antibody
  • Catalog No.
    DF2667
  • Source
    Rabbit
  • Application
    WB,IHC,ELISA
  • Reactivity
    Human, Mouse, Rat
  • Prediction
    Pig(100%), Bovine(100%), Horse(88%), Sheep(100%), Rabbit(100%), Dog(100%)
  • UniProt
  • Mol.Wt.
    51 kDa,52kDa
  • Concentration
    1mg/ml
  • Browse similar products>>

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Product Information

Alternative Names:Expand▼

IL-1 receptor-associated kinase 4; Interleukin 1 receptor associated kinase 4 mutant form 1; Interleukin-1 receptor-associated kinase 4; Interleukin1 receptor associated kinase 4; IPD1; IRAK 4; IRAK-4; IRAK4; IRAK4 mutated form 1; IRAK4_HUMAN; LOC 51135; NY REN 64; NY REN 64 antigen; NY-REN-64; REN64; Renal carcinoma antigen NY-REN-64;

Applications:

WB 1:500-1:2000, IHC 1:50-1:200, ELISA(peptide) 1:20000-1:40000

Reactivity:

Human, Mouse, Rat

Predicted Reactivity:

Pig(100%), Bovine(100%), Horse(88%), Sheep(100%), Rabbit(100%), Dog(100%)

Source:

Rabbit

Clonality:

Polyclonal

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Specificity:

IRAK4 Antibody detects endogenous levels of total IRAK4.

Format:

Liquid

Concentration:

1mg/ml

Storage Condition and Buffer:

Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Immunogen Information

Immunogen:

A synthesized peptide derived from human IRAK4, corresponding to a region within the internal amino acids.

Uniprot:



>>Visit The Human Protein Atlas

Gene ID:

Gene Name:

Q9NWZ3

Molecular Weight:

Observed Mol.Wt.: 51 kDa;.
Predicted Mol.Wt.: 52kDa.

Description:

Required for the efficient recruitment of IRAK1 to the IL-1 receptor complex following IL-1 engagement, triggering intracellular signaling cascades leading to transcriptional up-regulation and mRNA stabilization. Phosphorylates IRAK1.;

Sequence:
        10         20         30         40         50
MNKPITPSTY VRCLNVGLIR KLSDFIDPQE GWKKLAVAIK KPSGDDRYNQ
60 70 80 90 100
FHIRRFEALL QTGKSPTSEL LFDWGTTNCT VGDLVDLLIQ NEFFAPASLL
110 120 130 140 150
LPDAVPKTAN TLPSKEAITV QQKQMPFCDK DRTLMTPVQN LEQSYMPPDS
160 170 180 190 200
SSPENKSLEV SDTRFHSFSF YELKNVTNNF DERPISVGGN KMGEGGFGVV
210 220 230 240 250
YKGYVNNTTV AVKKLAAMVD ITTEELKQQF DQEIKVMAKC QHENLVELLG
260 270 280 290 300
FSSDGDDLCL VYVYMPNGSL LDRLSCLDGT PPLSWHMRCK IAQGAANGIN
310 320 330 340 350
FLHENHHIHR DIKSANILLD EAFTAKISDF GLARASEKFA QTVMTSRIVG
360 370 380 390 400
TTAYMAPEAL RGEITPKSDI YSFGVVLLEI ITGLPAVDEH REPQLLLDIK
410 420 430 440 450
EEIEDEEKTI EDYIDKKMND ADSTSVEAMY SVASQCLHEK KNKRPDIKKV
460
QQLLQEMTAS

Background

Function:

Serine/threonine-protein kinase that plays a critical role in initiating innate immune response against foreign pathogens. Involved in Toll-like receptor (TLR) and IL-1R signaling pathways (PubMed:17878374). Is rapidly recruited by MYD88 to the receptor-signaling complex upon TLR activation to form the Myddosome together with IRAK2. Phosphorylates initially IRAK1, thus stimulating the kinase activity and intensive autophosphorylation of IRAK1. Phosphorylates E3 ubiquitin ligases Pellino proteins (PELI1, PELI2 and PELI3) to promote pellino-mediated polyubiquitination of IRAK1. Then, the ubiquitin-binding domain of IKBKG/NEMO binds to polyubiquitinated IRAK1 bringing together the IRAK1-MAP3K7/TAK1-TRAF6 complex and the NEMO-IKKA-IKKB complex. In turn, MAP3K7/TAK1 activates IKKs (CHUK/IKKA and IKBKB/IKKB) leading to NF-kappa-B nuclear translocation and activation. Alternatively, phosphorylates TIRAP to promote its ubiquitination and subsequent degradation. Phosphorylates NCF1 and regulates NADPH oxidase activation after LPS stimulation suggesting a similar mechanism during microbial infections.

Post-translational Modifications:

Phosphorylated.

Subunit Structure:

Associates with MYD88 and IRAK2 to form a ternary complex called the Myddosome. Once phosphorylated, IRAK4 dissociates from the receptor complex and then associates with the TNF receptor-associated factor 6 (TRAF6), IRAK1, and PELI1; this intermediate complex is required for subsequent NF-kappa-B activation. Direct binding of SMAD6 to PELI1 prevents complex formation and hence negatively regulates IL1R-TLR signaling and eventually NF-kappa-B-mediated gene expression. Interacts with IL1RL1.

Similarity:

Belongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family. Pelle subfamily.

Research Fields

Research Fields:

· Environmental Information Processing > Signal transduction > NF-kappa B signaling pathway.(View pathway)
· Environmental Information Processing > Signal transduction > MAPK signaling pathway.(View pathway)
· Human Diseases > Infectious diseases: Bacterial > Pertussis.
· Human Diseases > Infectious diseases: Viral > Measles.
· Human Diseases > Infectious diseases: Parasitic > Chagas disease (American trypanosomiasis).
· Human Diseases > Infectious diseases: Bacterial > Tuberculosis.
· Human Diseases > Infectious diseases: Viral > Influenza A.
· Human Diseases > Infectious diseases: Parasitic > Leishmaniasis.
· Human Diseases > Infectious diseases: Parasitic > Toxoplasmosis.
· Organismal Systems > Immune system > Toll-like receptor signaling pathway.(View pathway)
· Organismal Systems > Nervous system > Neurotrophin signaling pathway.(View pathway)
· Organismal Systems > Immune system > NOD-like receptor signaling pathway.(View pathway)

Reference Citations:

1). Gan Y et al. Patchouli oil ameliorates 5-fluorouracil-induced intestinal mucositis in rats via protecting intestinal barrier and regulating water transport. J Ethnopharmacol 2019 Dec 25;250:112519 (PubMed: 31883475) [IF=3.414]

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Catalog Number :

DF2667-BP
(Blocking peptide available as DF2667-BP)

Price/Size :

$200/1mg.
Tips: For phospho antibody, we provide phospho peptide(0.5mg) and non-phospho peptide(0.5mg).

Function :

Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. This mechanism is useful when non-specific binding is an issue, for example, in Western blotting (immunoblot) and immunohistochemistry (IHC). By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific; Specific binding will be absent from the western blot or immunostaining performed with the neutralized antibody.

Format and storage :

Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 10 mg/ml.The purity is >90%,tested by HPLC and MS.Storage Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C.

Precautions :

This product is for research use only. Not for use in diagnostic or therapeutic procedures.

Pig
100%
Rabbit
100%
Sheep
100%
Dog
100%
Bovine
100%
Horse
88%
Chicken
0%
Zebrafish
0%
Xenopus
0%
High similarity Medium similarity Low similarity No similarity
Q9NWZ3 as Substrate
Site PTM Type Enzyme
M1 Acetylation
K3 Acetylation
K3 Ubiquitination
T6 Phosphorylation Q9NWZ3 (IRAK4)
S8 Phosphorylation Q9NWZ3 (IRAK4)
K33 Ubiquitination
K34 Acetylation
S114 Phosphorylation Q9NWZ3 (IRAK4)
K115 Ubiquitination
S150 Phosphorylation Q9NWZ3 (IRAK4)
S151 Phosphorylation Q9NWZ3 (IRAK4)
S152 Phosphorylation Q9NWZ3 (IRAK4)
S157 Phosphorylation
T208 Phosphorylation Q9NWZ3 (IRAK4)
T209 Phosphorylation Q9NWZ3 (IRAK4)
K313 Ubiquitination
S336 Phosphorylation
K338 Ubiquitination
T342 Phosphorylation Q9NWZ3 (IRAK4)
T345 Phosphorylation Q9NWZ3 (IRAK4)
S346 Phosphorylation Q9NWZ3 (IRAK4)
T352 Phosphorylation Q9NWZ3 (IRAK4)
K400 Sumoylation
K416 Ubiquitination
Q9NWZ3 as PTM Enzyme
Substrate Site Source
P14598 (NCF1) T133 Uniprot
P14598 (NCF1) T138 Uniprot
P14598 (NCF1) T153 Uniprot
P14598 (NCF1) S288 Uniprot
P14598 (NCF1) S320 Uniprot
P14598 (NCF1) S345 Uniprot
P14598 (NCF1) S348 Uniprot
P14598 (NCF1) T356 Uniprot
P14598 (NCF1) S359 Uniprot
P14598 (NCF1) S370 Uniprot
P51617-4 (IRAK1) S376 Uniprot
P51617-1 (IRAK1) T387 Uniprot
P58753 (TIRAP) T28 Uniprot
Q96FA3 (PELI1) S70 Uniprot
Q96FA3 (PELI1) S76 Uniprot
Q96FA3 (PELI1) S78 Uniprot
Q96FA3 (PELI1) T80 Uniprot
Q96FA3 (PELI1) S82 Uniprot
Q96FA3 (PELI1) T86 Uniprot
Q96FA3 (PELI1) T127 Uniprot
Q96FA3 (PELI1) T288 Uniprot
Q96FA3 (PELI1) S293 Uniprot
Q9NWZ3 (IRAK4) T6 Uniprot
Q9NWZ3 (IRAK4) S8 Uniprot
Q9NWZ3 (IRAK4) S114 Uniprot
Q9NWZ3 (IRAK4) S150 Uniprot
Q9NWZ3 (IRAK4) S151 Uniprot
Q9NWZ3 (IRAK4) S152 Uniprot
Q9NWZ3 (IRAK4) T208 Uniprot
Q9NWZ3 (IRAK4) T209 Uniprot
Q9NWZ3 (IRAK4) T342 Uniprot
Q9NWZ3 (IRAK4) T345 Uniprot
Q9NWZ3 (IRAK4) S346 Uniprot
Q9NWZ3 (IRAK4) T352 Uniprot
IMPORTANT: For western blots, incubate membrane with diluted antibody in 5% w/v milk , 1X TBS, 0.1% Tween®20 at 4°C with gentle shaking, overnight.

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