GPR40 Antibody | Affinity Biosciences

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Product:	GPR40 Antibody
Catalog:	DF2745
Description:	Rabbit polyclonal antibody to GPR40
Application:	ELISA(peptide)
Cited expt.:
Reactivity:	Human, Mouse, Rat
Prediction:	Pig, Bovine, Sheep, Rabbit, Dog
Mol.Wt.:	28 kDa(Observed); 31kD(Calculated).
Uniprot:	O14842
RRID:	AB_2839951

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$280	In stock
200ul	$350	In stock

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Control Products

#DF2745-BP

GPR40 Peptide

1mg

Related Downloads

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Reactivity:

Human, Mouse, Rat

Prediction:

Pig(100%), Bovine(%), Sheep(%), Rabbit(%), Dog(%)

Clonality:

Polyclonal

Specificity:

GPR40 Antibody detects endogenous levels of total GPR40.

RRID:

AB_2839951
Cite Format: Affinity Biosciences Cat# DF2745, RRID:AB_2839951.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin.

Storage:

Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

FFA1R; FFAR 1; Ffar1; FFAR1_HUMAN; Free fatty acid receptor 1; G protein coupled receptor 40; G-protein coupled receptor 40; GPCR40; GPR 40; GPR40;

Immunogens

Immunogen:

A synthesized peptide derived from human GPR40, corresponding to a region within the internal amino acids.

Uniprot:

O14842(FFAR1_HUMAN) >>Visit HPA database.

Gene(ID):

FFAR1(2864)

Expression:

O14842 FFAR1_HUMAN:

Detected in brain and pancreas. Detected in pancreatic beta cells.

Description:

Receptor for medium and long chain saturated and unsaturated fatty acids. Binding of the ligand increase intracellular calcium concentration and amplify glucose-stimulated insulin secretion. The activity of this receptor is mediated by G-proteins that activate phospholipase C. Seems to act through a G(q) and G(i)-mediated pathway.

Sequence:

O14842 FFAR1_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MDLPPQLSFGLYVAAFALGFPLNVLAIRGATAHARLRLTPSLVYALNLGCSDLLLTVSLPLKAVEALASGAWPLPASLCPVFAVAHFFPLYAGGGFLAALSAGRYLGAAFPLGYQAFRRPCYSWGVCAAIWALVLCHLGLVFGLEAPGGWLDHSNTSLGINTPVNGSPVCLEAWDPASAGPARFSLSLLLFFLPLAITAFCYVGCLRALARSGLTHRRKLRAAWVAGGALLTLLLCVGPYNASNVASFLYPNLGGSWRKLGLITGAWSVVLNPLVTGYLGRGPGLKTVCAARTQGGKSQK

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Pig

100

Dog

100

Rabbit

100

Bovine

Sheep

Horse

Xenopus

Zebrafish

Chicken

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

Research Backgrounds

O14842_FFAR1_HUMAN

Function:

G-protein coupled receptor for medium and long chain saturated and unsaturated fatty acids that plays an important role in glucose homeostasis. Fatty acid binding increases glucose-stimulated insulin secretion, and may also enhance the secretion of glucagon-like peptide 1 (GLP-1). May also play a role in bone homeostasis; receptor signaling activates pathways that inhibit osteoclast differentiation (By similarity). Ligand binding leads to a conformation change that triggers signaling via G-proteins that activate phospholipase C, leading to an increase of the intracellular calcium concentration. Seems to act through a G(q) and G(i)-mediated pathway.

Subcellular Location:

Cell membrane>Multi-pass membrane protein.

Tissue Specificity:

Detected in brain and pancreas. Detected in pancreatic beta cells.

Family&Domains:

Belongs to the G-protein coupled receptor 1 family.

Research Fields

· Organismal Systems > Endocrine system > Insulin secretion. (View pathway)

References

1). Targeting of G-protein coupled receptor 40 alleviates airway hyperresponsiveness through RhoA/ROCK1 signaling pathway in obese asthmatic mice. Respiratory Research, 2023 (PubMed: 36803977) [IF=4.7]

Application: WB Species: Mouse Sample: lung tissues

Fig. 6 DC260126 alleviates AHR in obese asthmatic mice through RhoA/ROCK1 signal pathway. A Within 24 h after last challenge, airway resistance was measured by inhalation of methacholine (0 to 50 mg/ml) to determine airway resistance (n = 6 per group). B Severity of inflammation cell infiltration in the airways was assessed by H&E staining, and semi-quantitative pathology scores were shown. Mouse lung tissues from different treatment groups were harvested, the protein expression of GPR40 (C), the activity of RhoA (GTP-RhoA) (D) and protein expression ROCK1 (E) were evaluated by western blot (n = 4 per group). The data were presented as the mean ± S.E.M. *P

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GPR40 Antibody - #DF2745