Collagen III Antibody | Affinity Biosciences

Product:	Collagen III Antibody
Catalog:	AF0136
Description:	Rabbit polyclonal antibody to Collagen III
Application:	WB IHC IF/ICC
Cited expt.:	WB, IHC, IF/ICC
Reactivity:	Human, Mouse, Rat
Prediction:	Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus
Mol.Wt.:	138kDa; 139kD(Calculated).
Uniprot:	P02461
RRID:	AB_2813770

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$280	In stock
200ul	$350	In stock

Lead Time: Same day delivery

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Related Downloads

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

IHC 1:50-1:200, IF/ICC 1:100-1:500, WB 1:1000
*The optimal dilutions should be determined by the end user. For optimal experimental results, antibody reuse is not recommended.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse,Rat

Prediction:

Pig(86%), Bovine(83%), Horse(83%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(100%), Xenopus(83%)

Clonality:

Polyclonal

Specificity:

Collagen III Antibody detects endogenous levels of total Collagen III.

RRID:

AB_2813770
Cite Format: Affinity Biosciences Cat# AF0136, RRID:AB_2813770.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

Alpha 1 type III collagen; Alpha1 (III) collagen; CO3A1_HUMAN; COL 3A1; COL3A1; Collagen alpha 1(III) chain; Collagen alpha-1(III) chain; Collagen III alpha 1 chain precursor; Collagen III alpha 1 polypeptide; Collagen type III alpha 1 (Ehlers Danlos syndrome type IV autosomal dominant); Collagen type III alpha 1; Collagen type III alpha 1 chain; Collagen type III alpha; Collagen, fetal; EDS4A; Ehlers Danlos syndrome type IV, autosomal dominant; Fetal collagen; Type III collagen;

Immunogens

Immunogen:

A synthesized peptide derived from human Collagen III, corresponding to a region within N-terminal amino acids.

Uniprot:

P02461(CO3A1_HUMAN) >>Visit HPA database.

Gene(ID):

COL3A1(1281)

Description:

CO3A1 Collagen type III occurs in most soft connective tissues along with type I collagen. Belongs to the fibrillar collagen family. Trimers of identical alpha 1(III) chains. The chains are linked to each other by interchain disulfide bonds. Trimers are also cross-linked via hydroxylysines. 2 isoforms of the human protein are produced by alternative splicing. Note: This description may include information from UniProtKB.

Sequence:

P02461 CO3A1_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MMSFVQKGSWLLLALLHPTIILAQQEAVEGGCSHLGQSYADRDVWKPEPCQICVCDSGSVLCDDIICDDQELDCPNPEIPFGECCAVCPQPPTAPTRPPNGQGPQGPKGDPGPPGIPGRNGDPGIPGQPGSPGSPGPPGICESCPTGPQNYSPQYDSYDVKSGVAVGGLAGYPGPAGPPGPPGPPGTSGHPGSPGSPGYQGPPGEPGQAGPSGPPGPPGAIGPSGPAGKDGESGRPGRPGERGLPGPPGIKGPAGIPGFPGMKGHRGFDGRNGEKGETGAPGLKGENGLPGENGAPGPMGPRGAPGERGRPGLPGAAGARGNDGARGSDGQPGPPGPPGTAGFPGSPGAKGEVGPAGSPGSNGAPGQRGEPGPQGHAGAQGPPGPPGINGSPGGKGEMGPAGIPGAPGLMGARGPPGPAGANGAPGLRGGAGEPGKNGAKGEPGPRGERGEAGIPGVPGAKGEDGKDGSPGEPGANGLPGAAGERGAPGFRGPAGPNGIPGEKGPAGERGAPGPAGPRGAAGEPGRDGVPGGPGMRGMPGSPGGPGSDGKPGPPGSQGESGRPGPPGPSGPRGQPGVMGFPGPKGNDGAPGKNGERGGPGGPGPQGPPGKNGETGPQGPPGPTGPGGDKGDTGPPGPQGLQGLPGTGGPPGENGKPGEPGPKGDAGAPGAPGGKGDAGAPGERGPPGLAGAPGLRGGAGPPGPEGGKGAAGPPGPPGAAGTPGLQGMPGERGGLGSPGPKGDKGEPGGPGADGVPGKDGPRGPTGPIGPPGPAGQPGDKGEGGAPGLPGIAGPRGSPGERGETGPPGPAGFPGAPGQNGEPGGKGERGAPGEKGEGGPPGVAGPPGGSGPAGPPGPQGVKGERGSPGGPGAAGFPGARGLPGPPGSNGNPGPPGPSGSPGKDGPPGPAGNTGAPGSPGVSGPKGDAGQPGEKGSPGAQGPPGAPGPLGIAGITGARGLAGPPGMPGPRGSPGPQGVKGESGKPGANGLSGERGPPGPQGLPGLAGTAGEPGRDGNPGSDGLPGRDGSPGGKGDRGENGSPGAPGAPGHPGPPGPVGPAGKSGDRGESGPAGPAGAPGPAGSRGAPGPQGPRGDKGETGERGAAGIKGHRGFPGNPGAPGSPGPAGQQGAIGSPGPAGPRGPVGPSGPPGKDGTSGHPGPIGPPGPRGNRGERGSEGSPGHPGQPGPPGPPGAPGPCCGGVGAAAIAGIGGEKAGGFAPYYGDEPMDFKINTDEIMTSLKSVNGQIESLISPDGSRKNPARNCRDLKFCHPELKSGEYWVDPNQGCKLDAIKVFCNMETGETCISANPLNVPRKHWWTDSSAEKKHVWFGESMDGGFQFSYGNPELPEDVLDVHLAFLRLLSSRASQNITYHCKNSIAYMDQASGNVKKALKLMGSNEGEFKAEGNSKFTYTVLEDGCTKHTGEWSKTVFEYRTRKAVRLPIVDIAPYDIGGPDQEFGVDVGPVCFL

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Sheep

100

Dog

100

Chicken

100

Rabbit

100

Pig

Horse

Bovine

Xenopus

Zebrafish

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

Research Backgrounds

P02461_CO3A1_HUMAN

Function:

Collagen type III occurs in most soft connective tissues along with type I collagen. Involved in regulation of cortical development. Is the major ligand of ADGRG1 in the developing brain and binding to ADGRG1 inhibits neuronal migration and activates the RhoA pathway by coupling ADGRG1 to GNA13 and possibly GNA12.

PTMs:

Proline residues at the third position of the tripeptide repeating unit (G-X-Y) are hydroxylated in some or all of the chains.

O-linked glycan consists of a Glc-Gal disaccharide bound to the oxygen atom of a post-translationally added hydroxyl group.

Subcellular Location:

Secreted>Extracellular space>Extracellular matrix.

Family&Domains:

The C-terminal propeptide, also known as COLFI domain, have crucial roles in tissue growth and repair by controlling both the intracellular assembly of procollagen molecules and the extracellular assembly of collagen fibrils. It binds a calcium ion which is essential for its function.

Belongs to the fibrillar collagen family.

Research Fields

· Human Diseases > Infectious diseases: Parasitic > Amoebiasis.

· Organismal Systems > Immune system > Platelet activation. (View pathway)

· Organismal Systems > Endocrine system > Relaxin signaling pathway.

· Organismal Systems > Digestive system > Protein digestion and absorption.

References

1). Long-term simulated microgravity fosters carotid aging-like changes via Piezo1. Cardiovascular research, 2024 (PubMed: 38271270) [IF=10.2]

2). An asymmetric chitosan scaffold for tendon tissue engineering: In vitro and in vivo evaluation with rat tendon stem/progenitor cells. Acta Biomaterialia, 2018 (PubMed: 29678676) [IF=9.4]

Application: IF/ICC Species: rat Sample: tendon

Fig. 6. | Analyses of tendon tissue by immunofluorescence. Fluorescence immunohistochemistry at the regenerated tendon in each group at 4 weeks. (A-D) The production of COL1; (E-H) The production of COL3; (I-L) The production of TNMD; (M-P) The production of CD31. Blue: DAPI (cell nuclei); Green:COL1/COL3/CD31; Red: TNMD. Scale bar = 100 μm.

Application: IHC Species: rat Sample: tendon

Fig. 5.| Detailed analyses of tendon tissue organization and immunohistochemistry. (A-E) DAPI staining and chart of calculated cell density. Data were mean ± S.D. * p < 0.05 vs. ED group. (F-Q)Representative images of regenerated tendon in each group at 4 weeks. Scale bar =100 μm.

3). Exosome from indoleamine 2,3-dioxygenase-overexpressing bone marrow mesenchymal stem cells accelerates repair process of ischemia/reperfusion-induced acute kidney injury by regulating macrophages polarization. Stem cell research & therapy, 2022 (PubMed: 35902956) [IF=7.5]

Application: WB Species: Mouse Sample:

Fig. 4 Effect of MSCs-Exo-IDO on renal fibrosis in IRI mouse model. A Kidney tissues were harvested from the four groups on day 28 after IRI (n = 3/group). Kidney tissues in different groups were obtained and histopathology was monitored by Masson staining. Arrowheads indicated the collagen deposition and fibrotic lesions. B Quantization diagram of panel A as shown in the right. C Western Blot was used to detect the expression of α-SMA, collagen I and collagen III in the above kidney tissues (n = 3/group). D Quantization diagram of panel C as shown in the right. The histogram is presented by Means ± SD. *P

4). Modified citrus pectin ameliorates myocardial fibrosis and inflammation via suppressing galectin-3 and TLR4/MyD88/NF-κB signaling pathway. Biomedicine & Pharmacotherapy, 2020 (PubMed: 32172066) [IF=6.9]

Application: IHC Species: rat Sample: heart

Fig. 5. |Effects of MCP on ISO-induced myocardial fibrosis in rats. (A and B) Immunohistochemical analysis of collagen I and collagen Ⅲ protein in heart cross sections of different groups on day 15 and day 22 (200× magnification). Scale bars 100 μm.

Application: WB Species: rat Sample: heart

Fig. 6. |Effects of MCP on the expression of collagen Ⅰ and collagen Ⅲ induced by ISO in rats. Protein and mRNA levels of collagen Ⅰ and collagen Ⅲ were analyzed by western blot and qRT-PCR in each group on day 15 and day 22. (A) Protein expression of collagen Ⅰ and collagen Ⅲ were measured by western blot.

5). Amphibian-derived peptide RL-RF10 ameliorates paraquat-induced pulmonary fibrosis injury. Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie, 2024 (PubMed: 38244328) [IF=6.9]

6). Protective role of Gentianella acuta on isoprenaline induced myocardial fibrosis in rats via inhibition of NF-κB pathway. BIOMEDICINE & PHARMACOTHERAPY, 2019 (PubMed: 30554111) [IF=6.9]

7). Gentianella acuta improves TAC-induced cardiac remodelling by regulating the Notch and PI3K/Akt/FOXO1/3 pathways. Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie, 2022 (PubMed: 35988427) [IF=6.9]

Application: WB Species: Rat Sample:

Fig. 5. G. acuta prevented TAC-induced myocardial fibrosis in rats. (A,B) Masson′s trichrome staining to observe the interstitial collagen deposition in the left ventricle; scale bars = 100 µm, and bar graph showing the percentage of collagen deposition. (C,D) The protein expression levels of α-SMA, collagen I, and collagen III are detected via western blotting and quantitative analysis of the relative protein expression of α-SMA, collagen I, and collagen III. GAPDH is used as a loading control. (n = 3). (E-G) The mRNA levels of α-SMA, collagen I, and collagen III are measured by Real-time PCR in each group, and relative mRNA levels normalise to GAPDH (n = 6). Significance: * p

8). Ethyl ferulate suppresses post-myocardial infarction myocardial fibrosis by inhibiting transforming growth factor receptor 1. Phytomedicine, 2023 (PubMed: 37801895) [IF=6.7]

9). Targeting the Akt/PI3K/mTOR signaling pathway for complete eradication of keloid disease by sunitinib. Apoptosis, 2022 (PubMed: 35802302) [IF=6.1]

10). Bi-layered Composite Scaffold for Repair of the Osteochondral Defects. Advances in Wound Care, 2021 (PubMed: 33076773) [IF=5.8]

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Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

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Collagen III Antibody - #AF0136