HSP105 Antibody | Affinity Biosciences

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IHC
Cites

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Western blot analysis on HeLa cell lysates using HSP105 Antibody,The lane on the left was treated with the antigen-specific peptide.

AF0617 at 1/100 staining human testis tissue sections by IHC-P.

Fig.

Product:	HSP105 Antibody
Catalog:	AF0617
Description:	Rabbit polyclonal antibody to HSP105
Application:	WB IHC IF/ICC
Reactivity:	Human, Mouse
Prediction:	Bovine, Horse, Sheep, Rabbit, Dog, Xenopus
Mol.Wt.:	100kDa; 97kD(Calculated).
Uniprot:	Q92598
RRID:	AB_2834147

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

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200ul	$350	In stock

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MS Report

HPLC Report

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse

Prediction:

Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Xenopus(83%)

Clonality:

Polyclonal

Specificity:

HSP105 Antibody detects endogenous levels of total HSP105.

RRID:

AB_2834147
Cite Format: Affinity Biosciences Cat# AF0617, RRID:AB_2834147.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

Antigen NY CO 25; Antigen NY-CO-25; DKFZp686M05240; Heat shock 105kD alpha; Heat shock 105kD; Heat shock 105kD beta; Heat shock 105kDa protein 1; Heat shock 105kDa protein; Heat shock 105kDa/110kDa protein 1; Heat shock 110 kDa protein; Heat shock 110kDa protein; Heat shock protein 105 kDa; HS105_HUMAN; HSP105; HSP105A; HSP105B; HSP110; HSPH 1; Hsph1; KIAA0201; NY CO 25;

Immunogens

Immunogen:

Uniprot:

Q92598(HS105_HUMAN) >>Visit HPA database.

Gene(ID):

HSPH1(10808)

Expression:

Q92598 HS105_HUMAN:

Highly expressed in testis. Present at lower levels in most brain regions, except cerebellum. Overexpressed in cancer cells.

Description:

HSP105 a molecular chaperone of the heat shock protein 70 family. Has ATPase activity. Expressed at especially high levels in mammalian brain and has been shown to suppress apoptosis in neuronal cells and to prevent the aggregation of proteins following heat shock. Known to interact with a variety of proteins including alpha-tubulin and the androgen receptor.

Sequence:

Q92598 HS105_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MSVVGLDVGSQSCYIAVARAGGIETIANEFSDRCTPSVISFGSKNRTIGVAAKNQQITHANNTVSNFKRFHGRAFNDPFIQKEKENLSYDLVPLKNGGVGIKVMYMGEEHLFSVEQITAMLLTKLKETAENSLKKPVTDCVISVPSFFTDAERRSVLDAAQIVGLNCLRLMNDMTAVALNYGIYKQDLPSLDEKPRIVVFVDMGHSAFQVSACAFNKGKLKVLGTAFDPFLGGKNFDEKLVEHFCAEFKTKYKLDAKSKIRALLRLYQECEKLKKLMSSNSTDLPLNIECFMNDKDVSGKMNRSQFEELCAELLQKIEVPLYSLLEQTHLKVEDVSAVEIVGGATRIPAVKERIAKFFGKDISTTLNADEAVARGCALQCAILSPAFKVREFSVTDAVPFPISLIWNHDSEDTEGVHEVFSRNHAAPFSKVLTFLRRGPFELEAFYSDPQGVPYPEAKIGRFVVQNVSAQKDGEKSRVKVKVRVNTHGIFTISTASMVEKVPTEENEMSSEADMECLNQRPPENPDTDKNVQQDNSEAGTQPQVQTDAQQTSQSPPSPELTSEENKIPDADKANEKKVDQPPEAKKPKIKVVNVELPIEANLVWQLGKDLLNMYIETEGKMIMQDKLEKERNDAKNAVEEYVYEFRDKLCGPYEKFICEQDHQNFLRLLTETEDWLYEEGEDQAKQAYVDKLEELMKIGTPVKVRFQEAEERPKMFEELGQRLQHYAKIAADFRNKDEKYNHIDESEMKKVEKSVNEVMEWMNNVMNAQAKKSLDQDPVVRAQEIKTKIKELNNTCEPVVTQPKPKIESPKLERTPNGPNIDKKEEDLEDKNNFGAEPPHQNGECYPNEKNSVNMDLD

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Horse

100

Bovine

100

Sheep

100

Dog

100

Rabbit

100

Xenopus

Pig

Zebrafish

Chicken

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q92598 As Substrate

Q92598

Site	PTM Type	Source
S2	Acetylation	Uniprot
S2	Phosphorylation	Uniprot
T25	Phosphorylation	Uniprot
S31	Phosphorylation	Uniprot
R33	Methylation	Uniprot
S43	Phosphorylation	Uniprot
K44	Ubiquitination	Uniprot
K53	Ubiquitination	Uniprot
K68	Acetylation	Uniprot
K68	Methylation	Uniprot
K68	Ubiquitination	Uniprot
K82	Ubiquitination	Uniprot
K84	Ubiquitination	Uniprot
Y89	Phosphorylation	Uniprot
K95	Ubiquitination	Uniprot
K102	Ubiquitination	Uniprot
K126	Ubiquitination	Uniprot
S132	Phosphorylation	Uniprot
T138	Phosphorylation	Uniprot
T149	Phosphorylation	Uniprot
K185	Ubiquitination	Uniprot
K194	Acetylation	Uniprot
K194	Ubiquitination	Uniprot
K221	Ubiquitination	Uniprot
K234	Acetylation	Uniprot
K234	Ubiquitination	Uniprot
K239	Ubiquitination	Uniprot
K249	Acetylation	Uniprot
K249	Ubiquitination	Uniprot
R265	Methylation	Uniprot
K272	Acetylation	Uniprot
K272	Ubiquitination	Uniprot
K274	Acetylation	Uniprot
K275	Ubiquitination	Uniprot
K295	Ubiquitination	Uniprot
K300	Ubiquitination	Uniprot
S304	Phosphorylation	Uniprot
Y322	Phosphorylation	Uniprot
K351	Ubiquitination	Uniprot
K356	Ubiquitination	Uniprot
K360	Acetylation	Uniprot
K360	Ubiquitination	Uniprot
T365	Phosphorylation	Uniprot
S384	Phosphorylation	Uniprot
K388	Methylation	Uniprot
K388	Ubiquitination	Uniprot
S429	Phosphorylation	Uniprot
K430	Acetylation	Uniprot
K430	Ubiquitination	Uniprot
T433	Phosphorylation	Uniprot
Y446	Phosphorylation	Uniprot
K458	Ubiquitination	Uniprot
S468	Phosphorylation	Uniprot
K471	Ubiquitination	Uniprot
K475	Ubiquitination	Uniprot
T486	Phosphorylation	Uniprot
S493	Phosphorylation	Uniprot
T494	Phosphorylation	Uniprot
T503	Phosphorylation	Uniprot
S554	Phosphorylation	Uniprot
S557	Phosphorylation	Uniprot
T561	Phosphorylation	Uniprot
S562	Phosphorylation	Uniprot
K588	Ubiquitination	Uniprot
K590	Ubiquitination	Uniprot
Y614	Phosphorylation	Uniprot
K626	Ubiquitination	Uniprot
K635	Ubiquitination	Uniprot
Y641	Phosphorylation	Uniprot
Y643	Phosphorylation	Uniprot
K648	Ubiquitination	Uniprot
K655	Ubiquitination	Uniprot
T670	Phosphorylation	Uniprot
T672	Phosphorylation	Uniprot
Y677	Phosphorylation	Uniprot
Y688	Phosphorylation	Uniprot
K691	Ubiquitination	Uniprot
K697	Ubiquitination	Uniprot
T700	Phosphorylation	Uniprot
K703	Ubiquitination	Uniprot
K714	Ubiquitination	Uniprot
K749	Ubiquitination	Uniprot
K772	Ubiquitination	Uniprot
K790	Ubiquitination	Uniprot
K804	Ubiquitination	Uniprot
K806	Ubiquitination	Uniprot
S809	Phosphorylation	Uniprot
K811	Acetylation	Uniprot
K811	Ubiquitination	Uniprot
T815	Phosphorylation	Uniprot
K823	Ubiquitination	Uniprot
K824	Ubiquitination	Uniprot
S852	Phosphorylation	Uniprot

Research Backgrounds

Q92598_HS105_HUMAN

Function:

Acts as a nucleotide-exchange factor (NEF) for chaperone proteins HSPA1A and HSPA1B, promoting the release of ADP from HSPA1A/B thereby triggering client/substrate protein release. Prevents the aggregation of denatured proteins in cells under severe stress, on which the ATP levels decrease markedly. Inhibits HSPA8/HSC70 ATPase and chaperone activities (By similarity).

PTMs:

Phosphorylation on Ser-509 may be important for regulation of the HSPA8/HSC70 chaperone activity.

Subcellular Location:

Cytoplasm.

Tissue Specificity:

Highly expressed in testis. Present at lower levels in most brain regions, except cerebellum. Overexpressed in cancer cells.

Subunit Structure:

Interacts with HSPA8/HSC70 (By similarity). Interacts with HSPA1A (via NBD) and HSPA1B (via NBD).

Family&Domains:

Belongs to the heat shock protein 70 family.

Research Fields

· Genetic Information Processing > Folding, sorting and degradation > Protein processing in endoplasmic reticulum. (View pathway)

References

1). Knockdown of HSP110 attenuates hypoxia-induced pulmonary hypertension in mice through suppression of YAP/TAZ-TEAD4 pathway. RESPIRATORY RESEARCH, 2022 (PubMed: 35986277) [IF=5.8]

Application: IF/ICC Species: Mouse Sample: lung tissues

Fig. 1HSP110 is upregulated in lung tissues of the hypoxia-induced PH in mice. a The RVSP of the normoxia and hypoxia groups in mice (N = 8). b H&E staining of pulmonary arteries in lung tissues in normoxia and hypoxia group (N = 8). Scale bars, 50 μm. c Quantification of ratio of pulmonary arterial medial thickness to total vessel size (media/CSA) (N = 8). Relative mRNA level (d) and protein level (e, f) of HSP110 in pulmonary arteries (N = 8). g Double immunofluorescence staining of YAP (green) and HSP110 (red) in pulmonary arteries (N = 8). Scale bars, 50 μm. White arrows pointed to HSP110 and YAP double-positive cells. h Double immunofluorescence staining of α-SMA (green) and HSP110 (red) in pulmonary arteries (N = 8). Scale bars, 50 μm. White arrows pointed to HSP110 and α-SMA double-positive cells. Data are means ± SD from 8 mice per group. *p

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HSP105 Antibody - #AF0617