NYREN18 Antibody - #AF0272

(2)  
Product: NYREN18 Antibody
Catalog: AF0272
Description: Rabbit polyclonal antibody to NYREN18
Application: WB IHC IF/ICC
Reactivity: Human, Mouse, Monkey
Prediction: Bovine, Sheep, Rabbit, Dog
Mol.Wt.: 70kDa; 71kD(Calculated).
Uniprot: Q9Y5A7
RRID: AB_2833353

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:3000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Monkey
Prediction:
Bovine(83%), Sheep(83%), Rabbit(92%), Dog(92%)
Clonality:
Polyclonal
Specificity:
NYREN18 Antibody detects endogenous levels of total NYREN18.
RRID:
AB_2833353
Cite Format: Affinity Biosciences Cat# AF0272, RRID:AB_2833353.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

BS4; NEDD8 ultimate buster 1; Negative regulator of ubiquitin like proteins 1; Negative regulator of ubiquitin-like proteins 1; NUB1; NUB1_HUMAN; NUB1L; NY REN 18; NYREN18; Renal carcinoma antigen NY-REN-18;

Immunogens

Immunogen:
Uniprot:

Q9Y5A7(NUB1_HUMAN) >>Visit HPA database.

Gene(ID):
NUB1(51667)
Expression:
Q9Y5A7 NUB1_HUMAN:

Widely expressed with lowest expression in the pancreas for isoform 1 and in leukocytes, liver, prostate and skeletal muscle for isoform 2.

Description:
NYREN18 Specific down-regulator of the NEDD8 conjugation system. Recruits NEDD8 and its conjugates to the proteasome for degradation. Isoform 1 promotes the degradation of NEDD8 more efficiently than isoform 2. Induced by beta and gamma interferons. Directly interacts with NEDD8 and PSMD4/S5a, a member of the regulatory subunit of the 26S proteasome.
Sequence:
MAQKKYLQAKLTQFLREDRIQLWKPPYTDENKKVGLALKDLAKQYSDRLECCENEVEKVIEEIRCKAIERGTGNDNYRTTGIATIEVFLPPRLKKDRKNLLETRLHITGRELRSKIAETFGLQENYIKIVINKKQLQLGKTLEEQGVAHNVKAMVLELKQSEEDARKNFQLEEEEQNEAKLKEKQIQRTKRGLEILAKRAAETVVDPEMTPYLDIANQTGRSIRIPPSERKALMLAMGYHEKGRAFLKRKEYGIALPCLLDADKYFCECCRELLDTVDNYAVLQLDIVWCYFRLEQLECLDDAEKKLNLAQKCFKNCYGENHQRLVHIKGNCGKEKVLFLRLYLLQGIRNYHSGNDVEAYEYLNKARQLFKELYIDPSKVDNLLQLGFTAQEARLGLRACDGNVDHAATHITNRREELAQIRKEEKEKKRRRLENIRFLKGMGYSTHAAQQVLHAASGNLDEALKILLSNPQMWWLNDSNPETDNRQESPSQENIDRLVYMGFDALVAEAALRVFRGNVQLAAQTLAHNGGSLPPELPLSPEDSLSPPATSPSDSAGTSSASTDEDMETEAVNEILEDIPEHEEDYLDSTLEDEEIIIAEYLSYVENRKSATKKN

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Rabbit
92
Dog
92
Bovine
83
Sheep
83
Pig
0
Horse
0
Xenopus
0
Zebrafish
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q9Y5A7 As Substrate

Site PTM Type Enzyme
K10 Ubiquitination
K39 Ubiquitination
K43 Ubiquitination
K58 Ubiquitination
T103 Phosphorylation
K115 Ubiquitination
Y126 Phosphorylation
K134 Ubiquitination
K140 Ubiquitination
K152 Ubiquitination
K159 Ubiquitination
K167 Ubiquitination
K180 Ubiquitination
K184 Ubiquitination
K198 Acetylation
K198 Ubiquitination
Y212 Phosphorylation
T219 Phosphorylation
S222 Phosphorylation
K231 Ubiquitination
Y239 Phosphorylation
K242 Ubiquitination
K248 Ubiquitination
K250 Ubiquitination
K306 Ubiquitination
K312 Ubiquitination
K315 Ubiquitination
K329 Ubiquitination
Y360 Phosphorylation
K365 Ubiquitination
K371 Ubiquitination
K379 Ubiquitination
S489 Phosphorylation
T569 Phosphorylation

Research Backgrounds

Function:

Specific down-regulator of the NEDD8 conjugation system. Recruits NEDD8, UBD, and their conjugates to the proteasome for degradation. Isoform 1 promotes the degradation of NEDD8 more efficiently than isoform 2.

Subcellular Location:

Nucleus.
Note: Predominantly nuclear.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Widely expressed with lowest expression in the pancreas for isoform 1 and in leukocytes, liver, prostate and skeletal muscle for isoform 2.

Subunit Structure:

Directly interacts with NEDD8 and PSMD4/S5a, a member of the regulatory subunit of the 26S proteasome. Isoform 1 binds to NEDD8 more efficiently than isoform 2. Interacts with AIPL1. The interaction with UBD via UBA domains facilitates the linking of UBD-conjugated target protein to the proteasome complex and accelerates UBD degradation and that of its conjugates.

Restrictive clause

 

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