AFfirm™ XPA Mouse Monoclonal Antibody - #BF9701

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Product: XPA Mouse Monoclonal Antibody
Catalog: BF9701
Description: Mouse monoclonal antibody to XPA
Application: WB
Reactivity: Human
Prediction: Pig, Zebrafish, Bovine, Horse, Sheep, Dog, Xenopus
Mol.Wt.: 40kDa(Observed); 31kD(Calculated).
Uniprot: P23025

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#BF9701-BP

XPA Monoclonal Peptide

1mg
Related Downloads
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Mouse
Application:
WB 1:500-1:3000
*The optimal dilutions should be determined by the end user. For optimal experimental results, antibody reuse is not recommended.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human
Clonality:
Monoclonal [AFfirm9701]
Specificity:
XPA Mouse Monoclonal Antibody detects endogenous levels of total XPA.
Conjugate:
Unconjugated.
Purification:
Affinity-chromatography.
Storage:
Mouse IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

DNA repair protein complementing XP A cells; DNA repair protein complementing XP-A cells; DNA repair protein complementing XPA cells; Excision repair controlling; Xeroderma pigmentosum 1; Xeroderma pigmentosum complementation group A; Xeroderma pigmentosum group A complementing protein; Xeroderma pigmentosum group A-complementing protein; XP 1; XP1; xpa; XPA_HUMAN; Xpac;

Immunogens

Immunogen:

A synthesized peptide derived from human XPA, corresponding to a region within the internal amino acids.

Uniprot:

P23025(XPA_HUMAN) >>Visit HPA database.

Gene(ID):
XPA(7507)
Expression:
P23025 XPA_HUMAN:

Expressed in various cell lines and in skin fibroblasts.

Description:
XPA Involved in DNA excision repair. Initiates repair by binding to damaged sites with various affinities, depending on the photoproduct and the transcriptional state of the region. Required for UV-induced CHK1 phosphorylation and the recruitment of CEP164 to cyclobutane pyrimidine dimmers (CPD), sites of DNA damage after UV irradiation.
Sequence:
MAAADGALPEAAALEQPAELPASVRASIERKRQRALMLRQARLAARPYSATAAAATGGMANVKAAPKIIDTGGGFILEEEEEEEQKIGKVVHQPGPVMEFDYVICEECGKEFMDSYLMNHFDLPTCDNCRDADDKHKLITKTEAKQEYLLKDCDLEKREPPLKFIVKKNPHHSQWGDMKLYLKLQIVKRSLEVWGSQEALEEAKEVRQENREKMKQKKFDKKVKELRRAVRSSVWKRETIVHQHEYGPEENLEDDMYRKTCTMCGHELTYEKM

Research Backgrounds

Function:

Involved in DNA excision repair. Initiates repair by binding to damaged sites with various affinities, depending on the photoproduct and the transcriptional state of the region. Required for UV-induced CHEK1 phosphorylation and the recruitment of CEP164 to cyclobutane pyrimidine dimmers (CPD), sites of DNA damage after UV irradiation.

PTMs:

ATR-dependent phosphorylation of XPA at Ser-196 is important for cell survival in response to UV damage.

Ubiquitinated by HERC2 leading to degradation by the proteasome.

Subcellular Location:

Nucleus.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Expressed in various cell lines and in skin fibroblasts.

Family&Domains:

Belongs to the XPA family.

Research Fields

· Genetic Information Processing > Replication and repair > Nucleotide excision repair.

· Human Diseases > Drug resistance: Antineoplastic > Platinum drug resistance.

Restrictive clause

 

Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

For Research Use Only.
Not for use in diagnostic or therapeutic procedures. Not for resale. Not for distribution without written consent. Affinity Biosciences will not be held responsible for patent infringement or other violations that may occur with the use of our products. Affinity Biosciences, Affinity Biosciences Logo and all other trademarks are the property of Affinity Biosciences LTD.