Affinity-Purified phospho-specific polyclonal antibody production $2500/ project(From Peptide Synthesis to ELISA titer assay)
Polyclonal phospho-specific Antibody Development Schedules
I. Peptide synthesis
1. Phosphospecific peptide synthesis (up to 15 amino acids)--10 mg used for phosphospecific epitope affinity purification, 10 mg used for antibody production, and 10 mg delivered to client
2. 10 mg non-phosphospecific peptide (up to 15 amino acids)used for affinity purification.
Conjugation of 10 mg phosphopeptide (using Cysteine) to KLH and BSA(for pep-elisa), 5mg non-phosphopeptide(for pep-elisa).
III. Immunization and anti-sera production
Eight-week protocol to produce antibodies in 2 rabbits.
Pre-immune 5 ml/per rabbit and total final bleeds from two rabbits (50 ml antiserum) will be provided to client.
Ⅳ.Peptide affinity purification and ELISA test.
Separating the phosphorylated antibodies from the non-phosphorylated
antibodies is performed by immuno-affinity purification, using two columns ,which does not cross-react with non-phosphospecific peptides
(confirmed by ELISA)
13 weeks ($2500)
All the products of this project shall become the exclusive property of the client at the conclusion of the project.Full confidentiality is guaranteed
Quotations and Ordering:
For quotation requests and questions, you may contact us by our secured online quotation Our customer service representatives are available 24 hours Monday through Sunday.
You may contact us anytime for assistance.