Product: EPHA7 Antibody
Catalog: AF0627
Description: Rabbit polyclonal antibody to EPHA7
Application: WB IF/ICC
Reactivity: Human, Mouse, Rat
Prediction: Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus
Mol.Wt.: 112kDa; 112kD(Calculated).
Uniprot: Q15375
RRID: AB_2834259

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 100ul $280 In stock
 200ul $350 In stock

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Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(100%), Zebrafish(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(100%), Xenopus(100%)
Clonality:
Polyclonal
Specificity:
EPHA7 Antibody detects endogenous levels of total EPHA7.
RRID:
AB_2834259
Cite Format: Affinity Biosciences Cat# AF0627, RRID:AB_2834259.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

Cek 11; Developmental kinase 1; EBK; EHK-3; EHK3; EK11; Embryonic brain kinase; EPH homology kinase 3; EPH-like kinase 11; Epha7; EPHA7_HUMAN; Ephrin receptor Eph A7; Ephrin type A receptor 7; Ephrin type-A receptor 7; hEK11; MDK 1; Receptor protein tyrosine kinase HEK 11; Tyrosine protein kinase receptor EHK 3;

Immunogens

Immunogen:
Uniprot:
Gene(ID):
Expression:
Q15375 EPHA7_HUMAN:

Widely expressed.

Description:
This gene belongs to the ephrin receptor subfamily of the protein-tyrosine kinase family. EPH and EPH-related receptors have been implicated in mediating developmental events, particularly in the nervous system. Receptors in the EPH subfamily typically have a single kinase domain and an extracellular region containing a Cys-rich domain and 2 fibronectin type III repeats. The ephrin receptors are divided into 2 groups based on the similarity of their extracellular domain sequences and their affinities for binding ephrin-A and ephrin-B ligands.
Sequence:
MVFQTRYPSWIILCYIWLLRFAHTGEAQAAKEVLLLDSKAQQTELEWISSPPNGWEEISGLDENYTPIRTYQVCQVMEPNQNNWLRTNWISKGNAQRIFVELKFTLRDCNSLPGVLGTCKETFNLYYYETDYDTGRNIRENLYVKIDTIAADESFTQGDLGERKMKLNTEVREIGPLSKKGFYLAFQDVGACIALVSVKVYYKKCWSIIENLAIFPDTVTGSEFSSLVEVRGTCVSSAEEEAENAPRMHCSAEGEWLVPIGKCICKAGYQQKGDTCEPCGRGFYKSSSQDLQCSRCPTHSFSDKEGSSRCECEDGYYRAPSDPPYVACTRPPSAPQNLIFNINQTTVSLEWSPPADNGGRNDVTYRILCKRCSWEQGECVPCGSNIGYMPQQTGLEDNYVTVMDLLAHANYTFEVEAVNGVSDLSRSQRLFAAVSITTGQAAPSQVSGVMKERVLQRSVELSWQEPEHPNGVITEYEIKYYEKDQRERTYSTVKTKSTSASINNLKPGTVYVFQIRAFTAAGYGNYSPRLDVATLEEATGKMFEATAVSSEQNPVIIIAVVAVAGTIILVFMVFGFIIGRRHCGYSKADQEGDEELYFHFKFPGTKTYIDPETYEDPNRAVHQFAKELDASCIKIERVIGAGEFGEVCSGRLKLPGKRDVAVAIKTLKVGYTEKQRRDFLCEASIMGQFDHPNVVHLEGVVTRGKPVMIVIEFMENGALDAFLRKHDGQFTVIQLVGMLRGIAAGMRYLADMGYVHRDLAARNILVNSNLVCKVSDFGLSRVIEDDPEAVYTTTGGKIPVRWTAPEAIQYRKFTSASDVWSYGIVMWEVMSYGERPYWDMSNQDVIKAIEEGYRLPAPMDCPAGLHQLMLDCWQKERAERPKFEQIVGILDKMIRNPNSLKTPLGTCSRPISPLLDQNTPDFTTFCSVGEWLQAIKMERYKDNFTAAGYNSLESVARMTIEDVMSLGITLVGHQKKIMSSIQTMRAQMLHLHGTGIQV

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Dog
100
Xenopus
100
Zebrafish
100
Chicken
100
Rabbit
100
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q15375 As Substrate

Site PTM Type Enzyme
T87 Phosphorylation
S91 Phosphorylation
Y201 Phosphorylation
Y202 Phosphorylation
S307 Phosphorylation
S308 Phosphorylation
S501 Phosphorylation
Y597 Phosphorylation
K601 Ubiquitination
K606 Ubiquitination
T607 Phosphorylation
Y608 Phosphorylation
T613 Phosphorylation
Y614 Phosphorylation
K634 Ubiquitination
K657 Ubiquitination
T666 Phosphorylation
K668 Ubiquitination
Y671 Phosphorylation
Y748 Phosphorylation
Y754 Phosphorylation
S768 Phosphorylation
S775 Phosphorylation
S780 Phosphorylation
Y791 Phosphorylation
T792 Phosphorylation
T794 Phosphorylation
K797 Ubiquitination
Y810 Phosphorylation
K892 Ubiquitination

Research Backgrounds

Function:

Receptor tyrosine kinase which binds promiscuously GPI-anchored ephrin-A family ligands residing on adjacent cells, leading to contact-dependent bidirectional signaling into neighboring cells. The signaling pathway downstream of the receptor is referred to as forward signaling while the signaling pathway downstream of the ephrin ligand is referred to as reverse signaling. Among GPI-anchored ephrin-A ligands, EFNA5 is a cognate/functional ligand for EPHA7 and their interaction regulates brain development modulating cell-cell adhesion and repulsion. Has a repellent activity on axons and is for instance involved in the guidance of corticothalamic axons and in the proper topographic mapping of retinal axons to the colliculus. May also regulate brain development through a caspase(CASP3)-dependent proapoptotic activity. Forward signaling may result in activation of components of the ERK signaling pathway including MAP2K1, MAP2K2, MAPK1 AND MAPK3 which are phosphorylated upon activation of EPHA7.

PTMs:

Phosphorylated.

Subcellular Location:

Cell membrane>Single-pass type I membrane protein.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Widely expressed.

Subunit Structure:

Heterotetramer upon binding of the ligand. The heterotetramer is composed of an ephrin dimer and a receptor dimer. Oligomerization is probably required to induce biological responses (By similarity). Interacts (via PDZ-binding motif) with GRIP1 and PICK1 (via PDZ domain) (By similarity).

Family&Domains:

Belongs to the protein kinase superfamily. Tyr protein kinase family. Ephrin receptor subfamily.

Research Fields

· Organismal Systems > Development > Axon guidance.   (View pathway)

References

1). Senescent immune cells accumulation promotes brown adipose tissue dysfunction during aging. Nature Communications (PubMed: 37268694) [IF=16.6]

Application: WB    Species: Mouse    Sample:

Fig. 5 Adipose RBM3 mediates the action of S100A8+ immune cells in sympathetic innervation. a Schematic diagram of experimental processes. This diagram was created with BioRender.com. b Representative images of TH staining in the BAT of mice injected with AAV-RBM3 or AAV-NC and transferred with S100A8+ immune cells. Scale bar, 100 µm. c Representative immunoblots of UCP1, TH, p-TH, TUBB3 and RBM3 in the BAT of mice injected with AAV-RBM3 or AAV-NC and transferred with S100A8+ immune cells (n = 3). d Relative mRNA levels of Rbm3, Th Ucp1, Ppargc1α, p16, and p21 in the BAT of mice injected with AAV-RBM3 or AAV-NC and transferred with S100A8+ immune cells (n = 5). e Oxygen consumption of mice injected with AAV-RBM3 or AAV-NC and transferred with S100A8+ immune cells or S100A8- immune cells (n = 6–7). f Core body temperature of mice injected with AAV-RBM3 or AAV-NC were transferred with S100A8+ immune cells and subjected to cold challenge (n = 5). g Venn diagram showing overlapped genes which are RBM3-bounded and differentially expressed upon Rbm3 knockdown. h KEGG analysis of the 909 overlapped genes shown in (g). i Heatmap of axon guidance-related gene expressions based on RNA-seq data of Rbm3 knockdown differentiated brown adipocytes. j Relative mRNA levels of Rbm3 and axon guidance-related genes Nrp1 and Epha7 in differentiated brown adipocytes transfected with si-Rbm3 or si-NC (n = 3). k Representative immunoblots of NRP1 and EPHA7 in differentiated brown adipocytes transfected with si-Rbm3 or si-NC (n = 4). l RNA immunoprecipitation (RIP) assay assessing RBM3 binding on 3´UTR of Nrp1 and Epha7 in differentiated brown adipocytes (n = 4). m Relative mRNA level of Nrp1 and Epha7 in si-Rbm3 or si-NC transfected brown adipocytes upon transcriptional inhibition with actinomycin D at indicated time (n = 3). n Representative images of TUBB3 staining in PC12 cells cocultured with differentiated brown adipocytes overexpressed with RBM3 and transfected with si-Nrp1 or si-Epha7 (n = 4). Scale bar, 100 µm. Data shown are representative of three independent experiments with similar results. n indicates the number of biologically independent samples examined. Data are shown as the mean ± SEM. Statistical differences were supposed to be significant when P 

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