Product: TRIP13 Antibody
Catalog: AF0570
Description: Rabbit polyclonal antibody to TRIP13
Application: WB IHC IF/ICC
Reactivity: Human, Mouse, Rat
Prediction: Pig, Bovine, Horse, Sheep, Dog, Chicken
Mol.Wt.: 48kDa; 49kD(Calculated).
Uniprot: Q15645
RRID: AB_2834261

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 100ul $280 In stock
 200ul $350 In stock

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Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000, IF/ICC 1:100-1:500, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(82%), Bovine(91%), Horse(100%), Sheep(91%), Dog(100%), Chicken(91%)
Clonality:
Polyclonal
Specificity:
TRIP13 Antibody detects endogenous levels of total TRIP13.
RRID:
AB_2834261
Cite Format: Affinity Biosciences Cat# AF0570, RRID:AB_2834261.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

16E1-BP; 16E1BP; Homo sapiens HPV16 E1 protein binding protein mRNA complete cds; HPV16 E1 protein binding protein; HPV16 E1 protein-binding protein; Human papillomavirus type 16 E1 protein binding protein; Human papillomavirus type 16 E1 protein-binding protein; Pachytene checkpoint protein 2 homolog; PCH2; Thyroid hormone receptor interactor 13; Thyroid receptor interacting protein 13; Thyroid receptor-interacting protein 13; TR-interacting protein 13; TRIP-13; Trip13; TRP13_HUMAN;

Immunogens

Immunogen:
Uniprot:
Gene(ID):
Description:
Thyroid receptor interacting proteins (TRIPs) specifically interact with the ligand binding domain of the thyroid receptor (TR). TRIP13 does not require the presence of thyroid hormone for its interaction.
Sequence:
MDEAVGDLKQALPCVAESPTVHVEVHQRGSSTAKKEDINLSVRKLLNRHNIVFGDYTWTEFDEPFLTRNVQSVSIIDTELKVKDSQPIDLSACTVALHIFQLNEDGPSSENLEEETENIIAANHWVLPAAEFHGLWDSLVYDVEVKSHLLDYVMTTLLFSDKNVNSNLITWNRVVLLHGPPGTGKTSLCKALAQKLTIRLSSRYRYGQLIEINSHSLFSKWFSESGKLVTKMFQKIQDLIDDKDALVFVLIDEVESLTAARNACRAGTEPSDAIRVVNAVLTQIDQIKRHSNVVILTTSNITEKIDVAFVDRADIKQYIGPPSAAAIFKIYLSCLEELMKCQIIYPRQQLLTLRELEMIGFIENNVSKLSLLLNDISRKSEGLSGRVLRKLPFLAHALYVQAPTVTIEGFLQALSLAVDKQFEERKKLAAYI

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Horse
100
Dog
100
Bovine
91
Sheep
91
Chicken
91
Pig
82
Xenopus
0
Zebrafish
0
Rabbit
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q15645 As Substrate

Site PTM Type Enzyme
M1 Acetylation
S18 Phosphorylation
K34 Ubiquitination
K35 Ubiquitination
S41 Phosphorylation
Y56 Phosphorylation
S74 Phosphorylation
K81 Ubiquitination
T183 Phosphorylation
K185 Ubiquitination
K190 Ubiquitination
K195 Ubiquitination
Y206 Phosphorylation
S216 Phosphorylation
K227 Ubiquitination
K231 Ubiquitination
K235 Ubiquitination
K288 Ubiquitination
K316 Ubiquitination
S367 Phosphorylation
S370 Phosphorylation
S377 Phosphorylation
S384 Phosphorylation

Research Backgrounds

Function:

Plays a key role in chromosome recombination and chromosome structure development during meiosis. Required at early steps in meiotic recombination that leads to non-crossovers pathways. Also needed for efficient completion of homologous synapsis by influencing crossover distribution along the chromosomes affecting both crossovers and non-crossovers pathways. Also required for development of higher-order chromosome structures and is needed for synaptonemal-complex formation. In males, required for efficient synapsis of the sex chromosomes and for sex body formation. Promotes early steps of the DNA double-strand breaks (DSBs) repair process upstream of the assembly of RAD51 complexes. Required for depletion of HORMAD1 and HORMAD2 from synapsed chromosomes (By similarity). Plays a role in mitotic spindle assembly checkpoint (SAC) activation.

Subunit Structure:

Specifically interacts with the ligand binding domain of the thyroid receptor (TR). This interaction does not require the presence of thyroid hormone for its interaction. Interacts with HPV16 E1. Interacts with proteasome subunit PSMA8; to participate in meiosis progression during spermatogenesis (By similarity).

Family&Domains:

Belongs to the AAA ATPase family. PCH2 subfamily.

References

1). MMP-9-mediated regulation of hypoxia-reperfusion injury-related neutrophil inflammation in an in vitro proximal tubular cell model. RENAL FAILURE, 2021 (PubMed: 34057033) [IF=3.0]

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