Product: NDF2 Antibody
Catalog: AF0635
Description: Rabbit polyclonal antibody to NDF2
Application: WB IHC IF/ICC
Reactivity: Human, Mouse, Rat
Prediction: Pig, Bovine, Horse, Dog
Mol.Wt.: 48kDa; 41kD(Calculated).
Uniprot: Q15784
RRID: AB_2834265

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 100ul $280 In stock
 200ul $350 In stock

Lead Time: Same day delivery

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Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000, IF/ICC 1:100-1:500, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(100%), Bovine(100%), Horse(100%), Dog(100%)
Clonality:
Polyclonal
Specificity:
NDF2 Antibody detects endogenous levels of total NDF2.
RRID:
AB_2834265
Cite Format: Affinity Biosciences Cat# AF0635, RRID:AB_2834265.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

bHLHa1; class A basic helix loop helix protein 1; Class A basic helix-loop-helix protein 1; NDF2_HUMAN; NDR2; NDRF; neuroD related factor; NeuroD-related factor; NeuroD2; neurogenic basic helix loop helix protein; neurogenic differentiation 2; Neurogenic differentiation factor 2; neuronal differentiation 2;

Immunogens

Immunogen:
Uniprot:
Gene(ID):
Description:
NEUROD2 Appears to mediate neuronal differentiation. Efficient DNA binding requires dimerization with another bHLH protein.
Sequence:
MLTRLFSEPGLLSDVPKFASWGDGEDDEPRSDKGDAPPPPPPAPGPGAPGPARAAKPVPLRGEEGTEATLAEVKEEGELGGEEEEEEEEEEGLDEAEGERPKKRGPKKRKMTKARLERSKLRRQKANARERNRMHDLNAALDNLRKVVPCYSKTQKLSKIETLRLAKNYIWALSEILRSGKRPDLVSYVQTLCKGLSQPTTNLVAGCLQLNSRNFLTEQGADGAGRFHGSGGPFAMHPYPYPCSRLAGAQCQAAGGLGGGAAHALRTHGYCAAYETLYAAAGGGGASPDYNSSEYEGPLSPPLCLNGNFSLKQDSSPDHEKSYHYSMHYSALPGSRPTGHGLVFGSSAVRGGVHSENLLSYDMHLHHDRGPMYEELNAFFHN

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Dog
100
Sheep
0
Xenopus
0
Zebrafish
0
Chicken
0
Rabbit
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q15784 As Substrate

Site PTM Type Enzyme
S7 Phosphorylation
S20 Phosphorylation
K156 Ubiquitination
Y169 Phosphorylation
S212 Phosphorylation

Research Backgrounds

Function:

Transcriptional regulator implicated in neuronal determination. Mediates calcium-dependent transcription activation by binding to E box-containing promoter. Critical factor essential for the repression of the genetic program for neuronal differentiation; prevents the formation of synaptic vesicle clustering at active zone to the presynaptic membrane in postmitotic neurons. Induces transcription of ZEB1, which in turn represses neuronal differentiation by down-regulating REST expression. Plays a role in the establishment and maturation of thalamocortical connections; involved in the segregation of thalamic afferents into distinct barrel domains within layer VI of the somatosensory cortex. Involved in the development of the cerebellar and hippocampal granular neurons, neurons in the basolateral nucleus of amygdala and the hypothalamic-pituitary axis. Associates with chromatin to the DPYSL3 E box-containing promoter (By similarity).

Subcellular Location:

Nucleus.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Subunit Structure:

Interacts with TCF3, TCF4 and TCF12. Interacts with CDC20. Efficient DNA-binding and transcription activation require dimerization with another bHLH protein (By similarity).

Family&Domains:

The C-terminal region is necessary for depolarization-induced and calcium-dependent transcription activation.

References

1). Comparative Metabolomics Analysis Reveals Key Metabolic Mechanisms and Protein Biomarkers in Alzheimer’s Disease. Frontiers in Pharmacology (PubMed: 35694256) [IF=5.6]

Application: IF/ICC    Species: Mouse    Sample: brain tissue

FIGURE 7 Experimental validation of HER2 and NDF2. (A) Immunofluorescence expression of HER2 (8.5 months). (B) Immunofluorescence expression of NDF2 (8.5 months). (C) Western blot results of NDF2 and HER2 (17 months). (D,E) Statistical analysis of immunofluorescence and western blot.

Application: WB    Species: Mouse    Sample: brain tissue

FIGURE 7 Experimental validation of HER2 and NDF2. (A) Immunofluorescence expression of HER2 (8.5 months). (B) Immunofluorescence expression of NDF2 (8.5 months). (C) Western blot results of NDF2 and HER2 (17 months). (D,E) Statistical analysis of immunofluorescence and western blot.

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Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

For Research Use Only.
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