STEAP4 Antibody - #AF0574

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Product: STEAP4 Antibody
Catalog: AF0574
Description: Rabbit polyclonal antibody to STEAP4
Application: WB IHC IF/ICC
Reactivity: Human
Prediction: Bovine, Horse, Sheep, Rabbit, Dog, Chicken
Mol.Wt.: 51kDa; 52kD(Calculated).
Uniprot: Q687X5
RRID: AB_2834285

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
IHC 1:50-1:200, IF/ICC 1:100-1:500, WB 1:500-1:2000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human
Prediction:
Bovine(83%), Horse(92%), Sheep(83%), Rabbit(83%), Dog(92%), Chicken(83%)
Clonality:
Polyclonal
Specificity:
STEAP4 Antibody detects endogenous levels of total STEAP4.
RRID:
AB_2834285
Cite Format: Affinity Biosciences Cat# AF0574, RRID:AB_2834285.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

alpha-induced protein 9; Metalloreductase STEAP4; six transmembrane prostate protein 2; Six-transmembrane epithelial antigen of prostate 4; SixTransMembrane protein of prostate 2; STAMP 2; STAMP2; STEA4_HUMAN; STEAP 4; STEAP family member 4; STEAP4; TIARP; TNFAIP 9; TNFAIP9; Tumor necrosis factor; tumor necrosis factor, alpha-induced protein 9; tumor necrosis-alpha-induced adipose-related protein;

Immunogens

Immunogen:
Uniprot:

Q687X5(STEA4_HUMAN) >>Visit HPA database.

Gene(ID):
STEAP4(79689)
Expression:
Q687X5 STEA4_HUMAN:

Ubiquitous. Highly expressed in adipose tissue. Expressed in placenta, lung, heart and prostate. Detected at lower levels in liver, skeletal muscle, pancreas, testis and small intestine. Highly expressed in joints of patients with rheumatoid arthritis and localized with CD68 cells, a marker for macrophages.

Description:
STEAP4 Metalloreductase that has the ability to reduce both Fe(3+) to Fe(2+) and Cu(2+) to Cu(1+). Uses NAD(+) as acceptor. Play a role in systemic metabolic homeostasis, integrating inflammatory and metabolic responses. Associated with obesity and insulin-resistance. Involved in inflammatory arthritis, through the regulation of inflammatory cytokines. Inhibits anchorage-independent cell proliferation. Belongs to the STEAP family.
Sequence:
MEKTCIDALPLTMNSSEKQETVCIFGTGDFGRSLGLKMLQCGYSVVFGSRNPQKTTLLPSGAEVLSYSEAAKKSGIIIIAIHREHYDFLTELTEVLNGKILVDISNNLKINQYPESNAEYLAHLVPGAHVVKAFNTISAWALQSGALDASRQVFVCGNDSKAKQRVMDIVRNLGLTPMDQGSLMAAKEIEKYPLQLFPMWRFPFYLSAVLCVFLFFYCVIRDVIYPYVYEKKDNTFRMAISIPNRIFPITALTLLALVYLPGVIAAILQLYRGTKYRRFPDWLDHWMLCRKQLGLVALGFAFLHVLYTLVIPIRYYVRWRLGNLTVTQAILKKENPFSTSSAWLSDSYVALGILGFFLFVLLGITSLPSVSNAVNWREFRFVQSKLGYLTLILCTAHTLVYGGKRFLSPSNLRWYLPAAYVLGLIIPCTVLVIKFVLIMPCVDNTLTRIRQGWERNSKH

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Horse
92
Dog
92
Bovine
83
Sheep
83
Chicken
83
Rabbit
83
Zebrafish
75
Xenopus
67
Pig
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q687X5 As Substrate

Site PTM Type Enzyme
K3 Ubiquitination
K18 Ubiquitination
S33 Phosphorylation
K54 Ubiquitination
S60 Phosphorylation
K72 Ubiquitination
K109 Ubiquitination
K161 Ubiquitination
T176 Phosphorylation
S182 Phosphorylation
K187 Ubiquitination
Y225 Phosphorylation
N323 N-Glycosylation
S408 Phosphorylation

Research Backgrounds

Function:

Integral membrane protein that functions as NADPH-dependent ferric-chelate reductase, using NADPH from one side of the membrane to reduce a Fe(3+) chelate that is bound on the other side of the membrane. Mediates sequential transmembrane electron transfer from NADPH to FAD and onto heme, and finally to the Fe(3+) chelate. Can also reduce Cu(2+) to Cu(1+) (By similarity). Plays a role in systemic metabolic homeostasis, integrating inflammatory and metabolic responses (By similarity). Associated with obesity and insulin-resistance. Involved in inflammatory arthritis, through the regulation of inflammatory cytokines. Inhibits anchorage-independent cell proliferation.

Subcellular Location:

Cell membrane>Multi-pass membrane protein. Golgi apparatus membrane>Multi-pass membrane protein. Early endosome membrane>Multi-pass membrane protein.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Ubiquitous. Highly expressed in adipose tissue. Expressed in placenta, lung, heart and prostate. Detected at lower levels in liver, skeletal muscle, pancreas, testis and small intestine. Highly expressed in joints of patients with rheumatoid arthritis and localized with CD68 cells, a marker for macrophages.

Subunit Structure:

Homotrimer. Interacts with PTK2/FAK1; the interaction may regulate PTK2 phosphorylation.

Family&Domains:

Belongs to the STEAP family.

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