Phospho-RAD9 (Ser272) Antibody - #AF8019

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Product: Phospho-RAD9 (Ser272) Antibody
Catalog: AF8019
Description: Rabbit polyclonal antibody to Phospho-RAD9 (Ser272)
Application: WB
Reactivity: Human
Mol.Wt.: 43kDa; 43kD(Calculated).
Uniprot: Q99638
RRID: AB_2840082

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:1000-3000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human
Clonality:
Polyclonal
Specificity:
Phospho-RAD9 (Ser272) Antibody detects endogenous levels of RAD9 only when phosphorylated at Ser272.
RRID:
AB_2840082
Cite Format: Affinity Biosciences Cat# AF8019, RRID:AB_2840082.
Conjugate:
Unconjugated.
Purification:
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

Cell cycle checkpoint control protein; Cell cycle checkpoint control protein RAD9A; DNA repair exonuclease rad9 homolog A; hRAD 9; hRAD9; Rad 9; RAD 9A; RAD9 (S pombe) homolog; RAD9 homolog A; RAD9 homolog; Rad9a; RAD9A_HUMAN;

Immunogens

Immunogen:
Uniprot:

Q99638(RAD9A_HUMAN) >>Visit HPA database.

Gene(ID):
RAD9A(5883)
Sequence:
MKCLVTGGNVKVLGKAVHSLSRIGDELYLEPLEDGLSLRTVNSSRSAYACFLFAPLFFQQYQAATPGQDLLRCKILMKSFLSVFRSLAMLEKTVEKCCISLNGRSSRLVVQLHCKFGVRKTHNLSFQDCESLQAVFDPASCPHMLRAPARVLGEAVLPFSPALAEVTLGIGRGRRVILRSYHEEEADSTAKAMVTEMCLGEEDFQQLQAQEGVAITFCLKEFRGLLSFAESANLNLSIHFDAPGRPAIFTIKDSLLDGHFVLATLSDTDSHSQDLGSPERHQPVPQLQAHSTPHPDDFANDDIDSYMIAMETTIGNEGSRVLPSISLSPGPQPPKSPGPHSEEEDEAEPSTVPGTPPPKKFRSLFFGSILAPVRSPQGPSPVLAEDSEGEG

PTMs - Q99638 As Substrate

Site PTM Type Enzyme
K2 Methylation
K15 Ubiquitination
Y28 Phosphorylation P00519 (ABL1) , A0A173G4P4 (Abl fusion)
K96 Ubiquitination
T167 Phosphorylation
S254 Phosphorylation
S266 Phosphorylation
S270 Phosphorylation
S272 Phosphorylation Q13315 (ATM)
S277 Phosphorylation P06493 (CDK1)
T292 Phosphorylation P06493 (CDK1)
T313 Phosphorylation
S324 Phosphorylation
S326 Phosphorylation
S328 Phosphorylation P06493 (CDK1) , Q9UKI8 (TLK1) , P24941 (CDK2)
S336 Phosphorylation P06493 (CDK1)
S341 Phosphorylation P68400 (CSNK2A1)
S350 Phosphorylation
T351 Phosphorylation
T355 Phosphorylation Q9UKI8-2 (TLK1) , P06493 (CDK1)
S363 Phosphorylation
S375 Phosphorylation
S380 Phosphorylation
S387 Phosphorylation P68400 (CSNK2A1)

Research Backgrounds

Function:

Component of the 9-1-1 cell-cycle checkpoint response complex that plays a major role in DNA repair. The 9-1-1 complex is recruited to DNA lesion upon damage by the RAD17-replication factor C (RFC) clamp loader complex. Acts then as a sliding clamp platform on DNA for several proteins involved in long-patch base excision repair (LP-BER). The 9-1-1 complex stimulates DNA polymerase beta (POLB) activity by increasing its affinity for the 3'-OH end of the primer-template and stabilizes POLB to those sites where LP-BER proceeds; endonuclease FEN1 cleavage activity on substrates with double, nick, or gap flaps of distinct sequences and lengths; and DNA ligase I (LIG1) on long-patch base excision repair substrates. The 9-1-1 complex is necessary for the recruitment of RHNO1 to sites of double-stranded breaks (DSB) occurring during the S phase. RAD9A possesses 3'->5' double stranded DNA exonuclease activity. Its phosphorylation by PRKCD may be required for the formation of the 9-1-1 complex.

PTMs:

Constitutively phosphorylated on serine and threonine amino acids in absence of DNA damage. Hyperphosphorylated by PRKCD and ABL1 upon DNA damage. Its phosphorylation by PRKCD may be required for the formation of the 9-1-1 complex.

Subcellular Location:

Nucleus.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Subunit Structure:

Component of the toroidal 9-1-1 (RAD9-RAD1-HUS1) complex, composed of RAD9A, RAD1 and HUS1. The 9-1-1 complex associates with LIG1, POLB, FEN1, RAD17, HDAC1, RPA1 and RPA2. The 9-1-1 complex associates with the RAD17-RFC complex. RAD9A interacts with BCL2L1, FEN1, PRKCD, RAD9B, HUS1, RAD1, ABL1, RPA1, ATAD5 and RPA2. Interacts with DNAJC7 and RHNO1.

Family&Domains:

Belongs to the rad9 family.

Research Fields

· Cellular Processes > Cell growth and death > Cellular senescence.   (View pathway)

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