Phospho-POU2F1/OCT1 (Ser385) Antibody - #AF8088
| Product: | Phospho-POU2F1/OCT1 (Ser385) Antibody |
| Catalog: | AF8088 |
| Description: | Rabbit polyclonal antibody to Phospho-POU2F1/OCT1 (Ser385) |
| Application: | WB |
| Reactivity: | Human, Mouse, Rat |
| Prediction: | Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus |
| Mol.Wt.: | 76KD; 76kD,51kD(Calculated). |
| Uniprot: | P14859 | P09086 |
| RRID: | AB_2840151 |
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Protocols
Product Info
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
Cite Format: Affinity Biosciences Cat# AF8088, RRID:AB_2840151.
Fold/Unfold
FLJ42836; NF A1; NF-A1; Oct 1; Oct 1B; Oct-1; OCT1; Octamer binding protein 1; Octamer binding transcription factor 1; Octamer-binding protein 1; Octamer-binding transcription factor 1; OTF 1; OTF-1; OTF1; OTTHUMP00000032348; OTTHUMP00000032350; OTTHUMP00000032351; PO21; PO2F1; PO2F1_HUMAN; POU class 2 homeobox 1; POU domain class 2 transcription factor 1; POU domain, class 2, transcription factor 1; POU2F1; class 2; Lymphoid restricted immunoglobulin octamer binding protein; Lymphoid-restricted immunoglobulin octamer-binding protein NF-A2; NF A2; Oct 2; Oct-2; Octamer binding transcription factor 2; Octamer-binding protein 2; Octamer-binding transcription factor 2; OTF-2; OTF2; PO2F2_HUMAN; POU domain; POU domain class 2 transcription factor 2; POU2F2; transcription factor 2;
Immunogens
Ubiquitous. Isoform 2 is lymphocyte-specific.
P09086 PO2F2_HUMAN:Isoform 3 is B-cell specific. Isoform 5 is expressed in B-cells and the immunoglobulin-expressing T-cell line MOLT-4, but not in the T-cell line BW5147.
- P14859 PO2F1_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MNNPSETSKPSMESGDGNTGTQTNGLDFQKQPVPVGGAISTAQAQAFLGHLHQVQLAGTSLQAAAQSLNVQSKSNEESGDSQQPSQPSQQPSVQAAIPQTQLMLAGGQITGLTLTPAQQQLLLQQAQAQAQLLAAAVQQHSASQQHSAAGATISASAATPMTQIPLSQPIQIAQDLQQLQQLQQQNLNLQQFVLVHPTTNLQPAQFIISQTPQGQQGLLQAQNLLTQLPQQSQANLLQSQPSITLTSQPATPTRTIAATPIQTLPQSQSTPKRIDTPSLEEPSDLEELEQFAKTFKQRRIKLGFTQGDVGLAMGKLYGNDFSQTTISRFEALNLSFKNMCKLKPLLEKWLNDAENLSSDSSLSSPSALNSPGIEGLSRRRKKRTSIETNIRVALEKSFLENQKPTSEEITMIADQLNMEKEVIRVWFCNRRQKEKRINPPSSGGTSSSPIKAIFPSPTSLVATTPSLVTSSAATTLTVSPVLPLTSAAVTNLSVTGTSDTTSNNTATVISTAPPASSAVTSPSLSPSPSASASTSEASSASETSTTQTTSTPLSSPLGTSQVMVTASGLQTAAAAALQGAAQLPANASLAAMAAAAGLNPSLMAPSQFAAGGALLSLNPGTLSGALSPALMSNSTLATIQALASGGSLPITSLDATGNLVFANAGGAPNIVTAPLFLNPQNLSLLTSNPVSLVSAAAASAGNSAPVASLHATSTSAESIQNSLFTVASASGAASTTTTASKAQ
- P09086 PO2F2_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MVHSSMGAPEIRMSKPLEAEKQGLDSPSEHTDTERNGPDTNHQNPQNKTSPFSVSPTGPSTKIKAEDPSGDSAPAAPLPPQPAQPHLPQAQLMLTGSQLAGDIQQLLQLQQLVLVPGHHLQPPAQFLLPQAQQSQPGLLPTPNLFQLPQQTQGALLTSQPRAGLPTQAVTRPTLPDPHLSHPQPPKCLEPPSHPEEPSDLEELEQFARTFKQRRIKLGFTQGDVGLAMGKLYGNDFSQTTISRFEALNLSFKNMCKLKPLLEKWLNDAETMSVDSSLPSPNQLSSPSLGFDGLPGRRRKKRTSIETNVRFALEKSFLANQKPTSEEILLIAEQLHMEKEVIRVWFCNRRQKEKRINPCSAAPMLPSPGKPASYSPHMVTPQGGAGTLPLSQASSSLSTTVTTLSSAVGTLHPSRTAGGGGGGGGAAPPLNSIPSVTPPPPATTNSTNPSPQGSHSAIGLSGLNPSTGPGLWWNPAPYQP
Predictions
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - P14859/P09086 As Substrate
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| S78 | Phosphorylation | P78527 (PRKDC) | Uniprot |
| S81 | Phosphorylation | P78527 (PRKDC) | Uniprot |
| S85 | Phosphorylation | P78527 (PRKDC) | Uniprot |
| S88 | Phosphorylation | P78527 (PRKDC) | Uniprot |
| S92 | Phosphorylation | P78527 (PRKDC) | Uniprot |
| T100 | Phosphorylation | P78527 (PRKDC) | Uniprot |
| S141 | Phosphorylation | P78527 (PRKDC) | Uniprot |
| S143 | Phosphorylation | P78527 (PRKDC) | Uniprot |
| S147 | Phosphorylation | P78527 (PRKDC) | Uniprot |
| T162 | Phosphorylation | P78527 (PRKDC) | Uniprot |
| S167 | Phosphorylation | P78527 (PRKDC) | Uniprot |
| T226 | Phosphorylation | P78527 (PRKDC) | Uniprot |
| S232 | Phosphorylation | P78527 (PRKDC) | Uniprot |
| T251 | Phosphorylation | Uniprot | |
| T255 | Phosphorylation | Uniprot | |
| T259 | Phosphorylation | Uniprot | |
| S267 | Phosphorylation | Uniprot | |
| S269 | Phosphorylation | P24941 (CDK2) | Uniprot |
| T270 | Phosphorylation | P24941 (CDK2) | Uniprot |
| K272 | Acetylation | Uniprot | |
| K272 | Ubiquitination | Uniprot | |
| T276 | Phosphorylation | Uniprot | |
| S278 | Phosphorylation | Uniprot | |
| S283 | Phosphorylation | Uniprot | |
| K293 | Ubiquitination | Uniprot | |
| K301 | Ubiquitination | Uniprot | |
| K315 | Ubiquitination | Uniprot | |
| S335 | Phosphorylation | Q9HC98 (NEK6) , Q9Y478 (PRKAB1) , Q13131 (PRKAA1) | Uniprot |
| S366 | Phosphorylation | Uniprot | |
| S370 | Phosphorylation | Uniprot | |
| S385 | Phosphorylation | Q13131 (PRKAA1) , P17612 (PRKACA) , P50613 (CDK7) | Uniprot |
| T445 | Phosphorylation | Uniprot | |
| S446 | Phosphorylation | Uniprot | |
| S447 | Phosphorylation | Uniprot | |
| S448 | Phosphorylation | Uniprot |
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| S4 | Phosphorylation | Uniprot | |
| S5 | Phosphorylation | Uniprot | |
| S14 | Phosphorylation | Uniprot | |
| S26 | Phosphorylation | Uniprot | |
| S53 | Phosphorylation | Uniprot | |
| S55 | Phosphorylation | Uniprot | |
| S192 | O-Glycosylation | Uniprot | |
| K216 | Ubiquitination | Uniprot | |
| K230 | Ubiquitination | Uniprot | |
| S250 | Phosphorylation | Uniprot | |
| S272 | O-Glycosylation | Uniprot | |
| S275 | O-Glycosylation | Uniprot | |
| T302 | O-Glycosylation | Uniprot | |
| T306 | Phosphorylation | Uniprot | |
| R309 | Methylation | Uniprot | |
| S324 | O-Glycosylation | Uniprot | |
| S372 | O-Glycosylation | Uniprot | |
| S390 | O-Glycosylation | Uniprot | |
| S395 | O-Glycosylation | Uniprot |
Research Backgrounds
Transcription factor that binds to the octamer motif (5'-ATTTGCAT-3') and activates the promoters of the genes for some small nuclear RNAs (snRNA) and of genes such as those for histone H2B and immunoglobulins. Modulates transcription transactivation by NR3C1, AR and PGR (By similarity). In case of human herpes simplex virus (HSV) infection, POU2F1 forms a multiprotein-DNA complex with the viral transactivator protein VP16 and HCFC1 thereby enabling the transcription of the viral immediate early genes.
Phosphorylated by PRKDC.
Nucleus.
Ubiquitous. Isoform 2 is lymphocyte-specific.
Interacts with NR3C1, AR, PGR and HCFC1. Associates with the herpes simplex virus VP16-induced complex; binding to HCFC1 activates the viral transcriptional activator VP16 for association with POU2F1, to form a multiprotein-DNA complex responsible for activating transcription of the viral immediate early genes.
Belongs to the POU transcription factor family. Class-2 subfamily.
Transcription factor that specifically binds to the octamer motif (5'-ATTTGCAT-3'). Regulates transcription in a number of tissues in addition to activating immunoglobulin gene expression. Modulates transcription transactivation by NR3C1, AR and PGR. Isoform 5 activates the U2 small nuclear RNA (snRNA) promoter.
Cytoplasm. Nucleus.
Isoform 3 is B-cell specific. Isoform 5 is expressed in B-cells and the immunoglobulin-expressing T-cell line MOLT-4, but not in the T-cell line BW5147.
Interacts with NR3C1, AR and PGR.
Belongs to the POU transcription factor family. Class-2 subfamily.
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