Phospho-CdGAP (Thr789) Antibody - #AF8252
(4)
| Product: | Phospho-CdGAP (Thr789) Antibody |
| Catalog: | AF8252 |
| Description: | Rabbit polyclonal antibody to Phospho-CdGAP (Thr789) |
| Application: | WB IHC |
| Reactivity: | Human, Mouse, Rat |
| Prediction: | Bovine, Sheep, Rabbit, Dog |
| Mol.Wt.: | 157KD; 157kD(Calculated). |
| Uniprot: | Q2M1Z3 |
| RRID: | AB_2840314 |
Related Downloads
Protocols
Product Info
Source:
Rabbit
Application:
WB 1:1000-3000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
Reactivity:
Human,Mouse,Rat
Prediction:
Bovine(100%), Sheep(100%), Rabbit(100%), Dog(100%)
Clonality:
Polyclonal
Specificity:
Phospho-CdGAP (Thr789) Antibody detects endogenous levels of CdGAP only when phosphorylated at Thr789.
RRID:
AB_2840314
Cite Format: Affinity Biosciences Cat# AF8252, RRID:AB_2840314.
Cite Format: Affinity Biosciences Cat# AF8252, RRID:AB_2840314.
Conjugate:
Unconjugated.
Purification:
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:
Fold/Unfold
ARHGAP31; Cdc42 GTPase-activating protein; RHG31_HUMAN; Rho GTPase-activating protein 31;
Immunogens
Immunogen:
Uniprot:
Gene(ID):
Sequence:
- Q2M1Z3 RHG31_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MKNKGAKQKLKRKGAASAFGCDLTEYLESSGQDVPYVLKSCAEFIETHGIVDGIYRLSGVTSNIQRLRQEFGSDQCPDLTREVYLQDIHCVGSLCKLYFRELPNPLLTYELYEKFTEAVSHCPEEGQLARIQNVIQELPPSHYRTLEYLIRHLAHIASFSSKTNMHARNLALVWAPNLLRSKEIEATGCNGDAAFLAVRVQQVVIEFILNHVDQIFNNGAPGSLENDENRPIMKSLTLPALSLPMKLVSLEEAQARSLATNHPARKERRENSLPEIVPPMGTLFHTVLELPDNKRKLSSKSKKWKSIFNLGRSGSDSKSKLSRNGSVFVRGQRLSVEKATIRPAKSMDSLCSVPVEGKETKGNFNRTVTTGGFFIPATKMHSTGTGSSCDLTKQEGEWGQEGMPPGAEGGFDVSSDRSHLQGAQARPPPEQLKVFRPVEDPESEQTAPKMLGMFYTSNDSPSKSVFTSSLFQMEPSPRNQRKALNISEPFAVSVPLRVSAVISTNSTPCRTPPKELQSLSSLEEFSFHGSESGGWPEEEKPLGAETSAASVPKKAGLEDAKAVPEAPGTVECSKGLSQEPGAHLEEKKTPESSLSSQHLNELEKRPNPEKVVEEGREAGEMESSTLQESPRARAEAVLLHEMDEDDLANALIWPEIQQELKIIESEEELSSLPPPALKTSPIQPILESSLGPFIPSEPPGSLPCGSFPAPVSTPLEVWTRDPANQSTQGASTAASREKPEPEQGLHPDLASLAPLEIVPFEKASPQATVEVGGPGNLSPPLPPAPPPPTPLEESTPVLLSKGGPEREDSSRKLRTDLYIDQLKSQDSPEISSLCQGEEATPRHSDKQNSKNAASEGKGCGFPSPTREVEIVSQEEEDVTHSVQEPSDCDEDDTVTDIAQHGLEMVEPWEEPQWVTSPLHSPTLKDAHKAQVQGLQGHQLEKRLSHRPSLRQSHSLDSKPTVKSQWTLEVPSSSSCANLETERNSDPLQPQAPRREITGWDEKALRSFREFSGLKGAEAPPNQKGPSGVQPNPAETSPISLAEGKELGTHLGHSSPQIRQGGVPGPESSKESSPSVQDSTSPGEHPAKLQLKSTECGPPKGKNRPSSLNLDPAIPIADLFWFENVASFSSPGMQVSEPGDPKVTWMTSSYCKADPWRVYSQDPQDLDIVAHALTGRRNSAPVSVSAVRTSFMVKMCQARAVPVIPPKIQYTQIPQPLPSQSSGENGVQPLERSQEGPSSTSGTTQKPAKDDSPSSLESSKEEKPKQDPGAIKSSPVDATAPCMCEGPTLSPEPGSSNLLSTQDAVVQCRKRMSETEPSGDNLLSSKLERPSGGSKPFHRSRPGRPQSLILFSPPFPIMDHLPPSSTVTDSKVLLSPIRSPTQTVSPGLLCGELAENTWVTPEGVTLRNKMTIPKNGQRLETSTSCFYQPQRRSVILDGRSGRQIE
Predictions
Predictions:
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
Species
Results
Score
Bovine
100
Sheep
100
Dog
100
Rabbit
100
Pig
0
Horse
0
Xenopus
0
Zebrafish
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - Q2M1Z3 As Substrate
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| T237 | Phosphorylation | Uniprot | |
| S242 | Phosphorylation | Uniprot | |
| S249 | Phosphorylation | Uniprot | |
| S272 | Phosphorylation | Uniprot | |
| S306 | Phosphorylation | Uniprot | |
| S326 | Phosphorylation | Uniprot | |
| S335 | Phosphorylation | Uniprot | |
| T340 | Phosphorylation | Uniprot | |
| S346 | Phosphorylation | Uniprot | |
| S349 | Phosphorylation | Uniprot | |
| S387 | Phosphorylation | Uniprot | |
| S388 | Phosphorylation | Uniprot | |
| S476 | Phosphorylation | Uniprot | |
| S499 | Phosphorylation | Uniprot | |
| S503 | Phosphorylation | Uniprot | |
| T511 | Phosphorylation | Uniprot | |
| S593 | Phosphorylation | Uniprot | |
| S629 | Phosphorylation | Uniprot | |
| S665 | Phosphorylation | Uniprot | |
| S778 | Phosphorylation | Uniprot | |
| T789 | Phosphorylation | P49840 (GSK3A) , P27361 (MAPK3) | Uniprot |
| S827 | Phosphorylation | Uniprot | |
| T840 | Phosphorylation | Uniprot | |
| S844 | Phosphorylation | Uniprot | |
| S849 | Phosphorylation | Uniprot | |
| S863 | Phosphorylation | Uniprot | |
| S920 | Phosphorylation | Uniprot | |
| S944 | Phosphorylation | Uniprot | |
| S948 | Phosphorylation | Uniprot | |
| S954 | Phosphorylation | Uniprot | |
| K1002 | Methylation | Uniprot | |
| S1036 | Phosphorylation | Uniprot | |
| S1053 | Phosphorylation | Uniprot | |
| S1080 | Phosphorylation | Uniprot | |
| S1106 | Phosphorylation | Uniprot | |
| S1178 | Phosphorylation | Uniprot | |
| S1182 | Phosphorylation | Uniprot | |
| S1184 | Phosphorylation | Uniprot | |
| S1312 | Phosphorylation | Uniprot | |
| S1317 | Phosphorylation | Uniprot | |
| S1339 | Phosphorylation | Uniprot | |
| S1374 | Phosphorylation | Uniprot | |
| S1432 | Phosphorylation | Uniprot |
Research Backgrounds
Function:
Functions as a GTPase-activating protein (GAP) for RAC1 and CDC42. Required for cell spreading, polarized lamellipodia formation and cell migration.
PTMs:
Phosphorylation on Thr-789 reduces GAP activity.
Subcellular Location:
Cell projection>Lamellipodium. Cell junction>Focal adhesion.
Subunit Structure:
Interacts with ITSN1, which inhibits GAP activity. Interacts with PARVA (By similarity). Interacts with GTP-loaded RHOU.
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