Phospho-Claudin 1 (Tyr210) Antibody - #AF8256
| Product: | Phospho-Claudin 1 (Tyr210) Antibody |
| Catalog: | AF8256 |
| Description: | Rabbit polyclonal antibody to Phospho-Claudin 1 (Tyr210) |
| Application: | WB IHC |
| Reactivity: | Human, Mouse, Rat |
| Prediction: | Pig, Bovine, Horse, Sheep, Rabbit, Chicken, Xenopus |
| Mol.Wt.: | 22kDa; 23kD(Calculated). |
| Uniprot: | O95832 |
| RRID: | AB_2840318 |
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Protocols
Product Info
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
Cite Format: Affinity Biosciences Cat# AF8256, RRID:AB_2840318.
Fold/Unfold
Claudin-1; Claudin1; CLD 1; CLD1; CLD1_HUMAN; CLDN 1; Cldn1; ILVASC; SEMP 1; SEMP1; Senescence associated epithelial membrane protein 1; Senescence associated epithelial membrane protein; Senescence-associated epithelial membrane protein;
Immunogens
Strongly expressed in liver and kidney. Expressed in heart, brain, spleen, lung and testis.
- O95832 CLD1_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MANAGLQLLGFILAFLGWIGAIVSTALPQWRIYSYAGDNIVTAQAMYEGLWMSCVSQSTGQIQCKVFDSLLNLSSTLQATRALMVVGILLGVIAIFVATVGMKCMKCLEDDEVQKMRMAVIGGAIFLLAGLAILVATAWYGNRIVQEFYDPMTPVNARYEFGQALFTGWAAASLCLLGGALLCCSCPRKTTSYPTPRPYPKPAPSSGKDYV
Predictions
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - O95832 As Substrate
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| K115 | Ubiquitination | Uniprot | |
| T153 | Phosphorylation | Uniprot | |
| K189 | Ubiquitination | Uniprot | |
| T191 | O-Glycosylation | Uniprot | |
| T191 | Phosphorylation | Uniprot | |
| S192 | O-Glycosylation | Uniprot | |
| S192 | Phosphorylation | Uniprot | |
| T195 | Phosphorylation | Uniprot | |
| K201 | Ubiquitination | Uniprot | |
| S205 | O-Glycosylation | Uniprot | |
| S205 | Phosphorylation | Uniprot | |
| S206 | O-Glycosylation | Uniprot | |
| S206 | Phosphorylation | Uniprot | |
| K208 | Ubiquitination | Uniprot | |
| Y210 | Phosphorylation | Uniprot |
Research Backgrounds
Claudins function as major constituents of the tight junction complexes that regulate the permeability of epithelia. While some claudin family members play essential roles in the formation of impermeable barriers, others mediate the permeability to ions and small molecules. Often, several claudin family members are coexpressed and interact with each other, and this determines the overall permeability. CLDN1 is required to prevent the paracellular diffusion of small molecules through tight junctions in the epidermis and is required for the normal barrier function of the skin. Required for normal water homeostasis and to prevent excessive water loss through the skin, probably via an indirect effect on the expression levels of other proteins, since CLDN1 itself seems to be dispensable for water barrier formation in keratinocyte tight junctions.
(Microbial infection) Acts as a receptor for hepatitis C virus (HCV) in hepatocytes. Associates with CD81 and the CLDN1-CD81 receptor complex is essential for HCV entry into host cell. Acts as a receptor for dengue virus.
Cell junction>Tight junction. Cell membrane>Multi-pass membrane protein. Basolateral cell membrane.
Note: Associates with CD81 and the CLDN1-CD81 complex localizes to the basolateral cell membrane.
Strongly expressed in liver and kidney. Expressed in heart, brain, spleen, lung and testis.
Homopolymers interact with CLDN3, but not CLDN2, homopolymers. Can form homo- and heteropolymers with other claudin family members. Directly interacts with TJP1/ZO-1, TJP2/ZO-2 and TJP3/ZO-3. Interacts with MPDZ and PATJ (By similarity). Interacts with OCLN, CLDN4, CLDN6 and CLDN9. Interacts with CD81.
(Microbial infection) Interacts with hepatitis c virus E1 and E2 proteins.
(Microbial infection) Interacts with dengue virus small envelope protein M.
Belongs to the claudin family.
Research Fields
· Cellular Processes > Cellular community - eukaryotes > Tight junction. (View pathway)
· Environmental Information Processing > Signaling molecules and interaction > Cell adhesion molecules (CAMs). (View pathway)
· Human Diseases > Infectious diseases: Bacterial > Pathogenic Escherichia coli infection.
· Human Diseases > Infectious diseases: Viral > Hepatitis C.
· Organismal Systems > Immune system > Leukocyte transendothelial migration. (View pathway)
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