Phospho-Kizuna (Thr379) Antibody | Affinity Biosciences

WB
pElisa

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Phospho-Kizuna (Thr379) Antibody - Western blot analysis of extracts from Rat brain, using Phospho-Kizuna (Thr379) Antibody at 1/1000 dilution.

Phospho-Kizuna (Thr379) Antibody - peptide-ELISA analysis of AF8277.

Product:	Phospho-Kizuna (Thr379) Antibody
Catalog:	AF8277
Description:	Rabbit polyclonal antibody to Phospho-Kizuna (Thr379)
Application:	WB
Reactivity:	Human
Prediction:	Rat, Pig, Xenopus
Mol.Wt.:	75KD; 75kD(Calculated).
Uniprot:	Q2M2Z5
RRID:	AB_2840339

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

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100ul	$350	In stock
200ul	$450	In stock

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Related Downloads

MS Report

HPLC Report

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:1000-3000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human

Prediction:

Rat(%), Pig(88%), Xenopus(88%)

Clonality:

Polyclonal

Specificity:

Phospho-Kizuna (Thr379) Antibody detects endogenous levels of Kizuna only when phosphorylated at Thr379.

RRID:

AB_2840339
Cite Format: Affinity Biosciences Cat# AF8277, RRID:AB_2840339.

Conjugate:

Unconjugated.

Purification:

The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

Centrosomal protein kizuna; DKFZP586H021; HT013; Kiz; KIZ_HUMAN; KIZUNA; MGC102941; MGC141930; NCRNA00153; Plk1s1; Polo-like kinase 1 substrate 1; Uncharacterized protein C20orf19;

Immunogens

Immunogen:

A synthesized peptide derived from human Kizuna around the phosphorylation site of Thr379.

Uniprot:

Q2M2Z5(KIZ_HUMAN) >>Visit HPA database.

Gene(ID):

KIZ(55857)

Sequence:

Q2M2Z5 KIZ_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MSRTLASAVPLSSPDYYERLGQLQHGLRDSEKKRLDLEKKLYEYNQSDTCRVKLKYVKLKNYLKEICESEKKAHTRNQEYLKRFERVQAHVVHFTTNTEKLQKLKLEYETQIKKMLCSKDSLGLKEELTDEDREKVAVHEGINSGTAMSRGLYQPATIFMGRQMSAILSMRDFSTEHKSPQPTKNFSIPDPHSHRQTAQSSNVTDSCVVQTSNDTQCLNKSDNIDGKASLQIGEKMPVTASVLSEEEQTHCLEIGSNTRHGKSNLSEGKKSAELNSPLRERLSPENRTTDLKCDSSSGSEGEILTREHIEVEEKRASPPVSPIPVSEYCESENKWSQEKHSPWEGVSDHLAHREPKSQKPFRKMQEEEEESWSTSSDLTISISEDDLILESPEPQPNPGGKMEGEDGIEALKLIHAEQERVALSTEKNCILQTLSSPDSEKESSTNAPTREPGQTPDSDVPRAQVGQHVATLKEHDNSVKEEATALLRKALTEECGRRSAIHSSESSCSLPSILNDNSGIKEAKPAVWLNSVPTREQEVSSGCGDKSKKENVAADIPITETEAYQLLKKATLQDNTNQTENRFQKTDASVSHLSGLNIGSGAFETKTANKIASEASFSSSEGSPLSRHENKKKPVINLKSNALWDESDDSNSEIEAALRPRNHNTDDSDDFYD

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Pig

Xenopus

Horse

Bovine

Sheep

Dog

Zebrafish

Chicken

Rabbit

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

Research Backgrounds

Q2M2Z5_KIZ_HUMAN

Function:

Centrosomal protein required for establishing a robust mitotic centrosome architecture that can endure the forces that converge on the centrosomes during spindle formation. Required for stabilizing the expanded pericentriolar material around the centriole.

PTMs:

Phosphorylation at Thr-379 by PLK1 is not needed for centrosomal localization or pericentriolar material expansion but is indispensable for spindle-pole stabilization.

Subcellular Location:

Cytoplasm>Cytoskeleton>Microtubule organizing center>Centrosome. Cytoplasm>Cytoskeleton>Cilium basal body.
Note: Localizes to centrosomes throughout the cell cycle. After centrosome duplication, it usually remains associated only with the mother centrosome, containing the older mature centriole and particles surrounding it. During prophase, additional particles accumulate around both separating centrosomes. Does not accumulate at the microtubule minus ends, but instead localizes to the centrosomes and centrosome-surrounding area in a microtubule-independent and dependent manner, respectively.

Family&Domains:

Belongs to the kizuna family.

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For Research Use Only.
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Phospho-Kizuna (Thr379) Antibody - #AF8277