IRX2 Antibody - #AF0552
(9)
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Protocols
Product Info
Source:
Rabbit
Application:
WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
Reactivity:
Human,Mouse,Rat
Prediction:
Pig(100%), Horse(100%), Rabbit(100%), Dog(100%)
Clonality:
Polyclonal
Specificity:
IRX2 Antibody detects endogenous levels of total IRX2.
RRID:
AB_2834315
Cite Format: Affinity Biosciences Cat# AF0552, RRID:AB_2834315.
Cite Format: Affinity Biosciences Cat# AF0552, RRID:AB_2834315.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:
Fold/Unfold
Homeodomain protein IRXA2; Iroquois homeobox 2; Iroquois homeobox protein 2; Iroquois-class homeodomain protein irx-2; IRX 2; irx2; IRX2_HUMAN; IrxA2;
Immunogens
Immunogen:
Uniprot:
Gene(ID):
Description:
IRX1 is a member of the Iroquois homeobox gene family. Members of this family appear to play multiple roles during pattern formation of vertebrate embryos.
Sequence:
- Q9BZI1 IRX2_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MSYPQGYLYQAPGSLALYSCPAYGASALAAPRSEELARSASGSAFSPYPGSAAFTAQAATGFGSPLQYSADAAAAAAGFPSYMGAPYDAHTTGMTGAISYHPYGSAAYPYQLNDPAYRKNATRDATATLKAWLNEHRKNPYPTKGEKIMLAIITKMTLTQVSTWFANARRRLKKENKMTWAPRNKSEDEDEDEGDATRSKDESPDKAQEGTETSAEDEGISLHVDSLTDHSCSAESDGEKLPCRAGDPLCESGSECKDKYDDLEDDEDDDEEGERGLAPPKPVTSSPLTGLEAPLLSPPPEAAPRGGRKTPQGSRTSPGAPPPASKPKLWSLAEIATSDLKQPSLGPGCGPPGLPAAAAPASTGAPPGGSPYPASPLLGRPLYYTSPFYGNYTNYGNLNAALQGQGLLRYNSAAAAPGEALHTAPKAASDAGKAGAHPLESHYRSPGGGYEPKKDASEGCTVVGGGVQPYL
Predictions
Predictions:
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
Species
Results
Score
Pig
100
Horse
100
Dog
100
Rabbit
100
Chicken
67
Bovine
0
Sheep
0
Xenopus
0
Zebrafish
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - Q9BZI1 As Substrate
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| S46 | Phosphorylation | P28482 (MAPK1) | Uniprot |
| S64 | Phosphorylation | P28482 (MAPK1) | Uniprot |
| S186 | Phosphorylation | Uniprot | |
| S231 | Phosphorylation | Uniprot | |
| S233 | Phosphorylation | Uniprot | |
| S236 | Phosphorylation | Uniprot | |
| S252 | Phosphorylation | Uniprot | |
| S254 | Phosphorylation | Uniprot | |
| T310 | Phosphorylation | Uniprot | |
| S317 | Phosphorylation | P27361 (MAPK3) | Uniprot |
| S325 | Phosphorylation | Uniprot | |
| S338 | Phosphorylation | Uniprot |
Research Backgrounds
Subcellular Location:
Nucleus.
Family&Domains:
Belongs to the TALE/IRO homeobox family.
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For Research Use Only.
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