Phospho-NACA (Thr159) Antibody - #AF8291
| Product: | Phospho-NACA (Thr159) Antibody |
| Catalog: | AF8291 |
| Description: | Rabbit polyclonal antibody to Phospho-NACA (Thr159) |
| Application: | WB IHC |
| Reactivity: | Human, Mouse, Rat |
| Prediction: | Pig, Zebrafish, Bovine, Sheep, Chicken, Xenopus |
| Mol.Wt.: | 24KD; 23kD(Calculated). |
| Uniprot: | Q13765 | Q9H009 |
| RRID: | AB_2840353 |
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Protocols
Product Info
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
Cite Format: Affinity Biosciences Cat# AF8291, RRID:AB_2840353.
Fold/Unfold
Alpha NAC; Alpha-NAC; Hom s 2.02; HSD48; MGC117224; NAC-alpha; naca; NACA/1.9.2; NACA_HUMAN; NACA1; Nascent polypeptide associated complex alpha polypeptide; Nascent polypeptide associated complex alpha subunit; Nascent polypeptide associated complex subunit alpha; Nascent polypeptide-associated complex subunit alpha; Alpha NAC like; ANAC; Hom s 2.01; MGC71999; NAC alpha like; NACA2; NACAL; nascent polypeptide associated complex alpha polypeptide like; Nascent polypeptide associated complex subunit alpha 2;
Immunogens
A synthesized peptide derived from human NACA around the phosphorylation site of Thr159.
Ubiquitously expressed.
Q9H009 NACA2_HUMAN:Expressed specifically in testis and skeletal muscle.
- Q13765 NACA_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MPGEATETVPATEQELPQPQAETGSGTESDSDESVPELEEQDSTQATTQQAQLAAAAEIDEEPVSKAKQSRSEKKARKAMSKLGLRQVTGVTRVTIRKSKNILFVITKPDVYKSPASDTYIVFGEAKIEDLSQQAQLAAAEKFKVQGEAVSNIQENTQTPTVQEESEEEEVDETGVEVKDIELVMSQANVSRAKAVRALKNNSNDIVNAIMELTM
- Q9H009 NACA2_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MPGEATETVPATEQELPQSQAETGSGTASDSGESVPGIEEQDSTQTTTQKAWLVAAAEIDEEPVGKAKQSRSEKRARKAMSKLGLLQVTGVTRVTIWKSKNILFVITKLDVYKSPASDAYIVFGEAKIQDLSQQAQLAAAEKFRVQGEAVGNIQENTQTPTVQEESEEEEVDETGVEVKDVKLVMSQANVSRAKAVRALKNNSNDIVNAIMELTV
Predictions
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
High(score>80) Medium(80>score>50) Low(score<50) No confidence
Research Backgrounds
Prevents inappropriate targeting of non-secretory polypeptides to the endoplasmic reticulum (ER). Binds to nascent polypeptide chains as they emerge from the ribosome and blocks their interaction with the signal recognition particle (SRP), which normally targets nascent secretory peptides to the ER. Also reduces the inherent affinity of ribosomes for protein translocation sites in the ER membrane (M sites). May act as a specific coactivator for JUN, binding to DNA and stabilizing the interaction of JUN homodimers with target gene promoters.
Phosphorylation of Thr-159 by GSK3B may promote proteasome mediated degradation (By similarity). Phosphorylation of Ser-43 by ILK during cell adhesion may promote nuclear localization.
Cytoplasm. Nucleus.
Note: The heterodimer is located mainly in the cytosol, and the homodimer in the nucleus.
Ubiquitously expressed.
The positively charged inner surface of the NAC-A/B domain is crucial for NACA localization in the nucleus and DNA-binding. This region is blocked from binding nucleic acids in the heterodimeric complex by a helix region in the beta-subunit, it also displays much higher affinity for RNA than DNA.
Belongs to the NAC-alpha family.
Prevents inappropriate targeting of non-secretory polypeptides to the endoplasmic reticulum (ER). Binds to nascent polypeptide chains as they emerge from the ribosome and blocks their interaction with the signal recognition particle (SRP), which normally targets nascent secretory peptides to the ER. Also reduces the inherent affinity of ribosomes for protein translocation sites in the ER membrane (M sites) (By similarity).
Cytoplasm. Nucleus.
Expressed specifically in testis and skeletal muscle.
Belongs to the NAC-alpha family.
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