Product: ZNF436 Antibody
Catalog: AF0427
Description: Rabbit polyclonal antibody to ZNF436
Application: WB IHC IF/ICC
Reactivity: Human, Monkey
Prediction: Pig, Bovine, Horse, Sheep, Dog
Mol.Wt.: 50kDa; 54kD(Calculated).
Uniprot: Q9C0F3
RRID: AB_2834317

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 100ul $280 In stock
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Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000, IF/ICC 1:100-1:500, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Monkey
Prediction:
Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Dog(100%)
Clonality:
Polyclonal
Specificity:
ZNF436 Antibody detects endogenous levels of total ZNF436.
RRID:
AB_2834317
Cite Format: Affinity Biosciences Cat# AF0427, RRID:AB_2834317.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

HGNC:20814; KIAA1710; Zfp46; Zinc finger protein 436; ZN436_HUMAN; ZNF; ZNF436;

Immunogens

Immunogen:
Uniprot:
Gene(ID):
Expression:
Q9C0F3 ZN436_HUMAN:

Expressed in fetal brain, heart, liver, spleen, bladder, lung, skin, skeletal muscle, stomach and pancreas.

Description:
ZNF436 May be a transcriptional repressor. Belongs to the krueppel C2H2-type zinc-finger protein family.
Sequence:
MAATLLMAGSQAPVTFEDMAMYLTREEWRPLDAAQRDLYRDVMQENYGNVVSLDFEIRSENEVNPKQEISEDVQFGTTSERPAENAEENPESEEGFESGDRSERQWGDLTAEEWVSYPLQPVTDLLVHKEVHTGIRYHICSHCGKAFSQISDLNRHQKTHTGDRPYKCYECGKGFSRSSHLIQHQRTHTGERPYDCNECGKSFGRSSHLIQHQTIHTGEKPHKCNECGKSFCRLSHLIQHQRTHSGEKPYECEECGKSFSRSSHLAQHQRTHTGEKPYECNECGRGFSERSDLIKHYRVHTGERPYKCDECGKNFSQNSDLVRHRRAHTGEKPYHCNECGENFSRISHLVQHQRTHTGEKPYECNACGKSFSRSSHLITHQKIHTGEKPYECNECWRSFGERSDLIKHQRTHTGEKPYECVQCGKGFTQSSNLITHQRVHTGEKPYECTECEKSFSRSSALIKHKRVHTD

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Dog
100
Xenopus
0
Zebrafish
0
Chicken
0
Rabbit
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q9C0F3 As Substrate

Site PTM Type Enzyme
S10 Phosphorylation
K167 Sumoylation
T271 Phosphorylation
T273 Phosphorylation
T301 Phosphorylation
T355 Phosphorylation
T357 Phosphorylation
T385 Phosphorylation
T411 Phosphorylation
T413 Phosphorylation
Y418 Phosphorylation
K425 Sumoylation
T441 Phosphorylation
S456 Phosphorylation
K465 Methylation

Research Backgrounds

Function:

May be a transcriptional repressor.

Subcellular Location:

Nucleus.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Expressed in fetal brain, heart, liver, spleen, bladder, lung, skin, skeletal muscle, stomach and pancreas.

Family&Domains:

Belongs to the krueppel C2H2-type zinc-finger protein family.

References

1). Chen Z et al. Expressions of ZNF436, β-catenin, EGFR, and CMTM5 in breast cancer and their clinical significances. EUROPEAN JOURNAL OF HISTOCHEMISTRY 2021 Jan 20;65(1). (PubMed: 33478201) [IF=2.0]

Application: IHC    Species: Human    Sample: breast cancer and paracancerous tissues

Figure 1.Expression levels of ZNF436, β-catenin, EGFR and CMTM5 in breast cancer and paracancerous tissues. The expression levels of ZNF436, β-catenin and EGFR in breast cancer tissues were significantly higher than those in adjacent tissues (p<0.05); CMTM5 was highly expressed in adjacent tissues (p<0.05).

Restrictive clause

 

Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

For Research Use Only.
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