Phospho-HtrA2 (Ser142) Antibody - #AF8381

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Product: Phospho-HtrA2 (Ser142) Antibody
Catalog: AF8381
Description: Rabbit polyclonal antibody to Phospho-HtrA2 (Ser142)
Application: WB IHC
Reactivity: Human, Mouse, Rat
Prediction: Pig, Bovine, Horse, Sheep, Rabbit, Dog
Mol.Wt.: 49kDa; 49kD(Calculated).
Uniprot: O43464
RRID: AB_2840442

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:1000-3000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(89%), Bovine(89%), Horse(89%), Sheep(89%), Rabbit(89%), Dog(89%)
Clonality:
Polyclonal
Specificity:
Phospho-HtrA2 (Ser142) Antibody detects endogenous levels of HtrA2 only when phosphorylated at Ser142.
RRID:
AB_2840442
Cite Format: Affinity Biosciences Cat# AF8381, RRID:AB_2840442.
Conjugate:
Unconjugated.
Purification:
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

High temperature requirement protein A2; HTRA 2; HtrA like serine protease; HtrA serine peptidase 2; HtrA, E. coli, homolog of, 2; HtrA2; HTRA2_HUMAN; mitochondrial; Omi stress regulated endoprotease; Omi stress-regulated endoprotease; PARK 13; PARK13; Protease serine 25; PRSS 25; PRSS25; Serine protease 25; Serine protease HTRA2; Serine protease HTRA2 mitochondrial; Serine protease htra2 mitochondrial precursor; Serine protease omi; Serine proteinase OMI;

Immunogens

Immunogen:
Uniprot:

O43464(HTRA2_HUMAN) >>Visit HPA database.

Gene(ID):
HTRA2(27429)
Expression:
O43464 HTRA2_HUMAN:

Isoform 1 is ubiquitous. Isoform 2 is expressed predominantly in the kidney, colon and thyroid.

Sequence:
MAAPRAGRGAGWSLRAWRALGGIRWGRRPRLTPDLRALLTSGTSDPRARVTYGTPSLWARLSVGVTEPRACLTSGTPGPRAQLTAVTPDTRTREASENSGTRSRAWLAVALGAGGAVLLLLWGGGRGPPAVLAAVPSPPPASPRSQYNFIADVVEKTAPAVVYIEILDRHPFLGREVPISNGSGFVVAADGLIVTNAHVVADRRRVRVRLLSGDTYEAVVTAVDPVADIATLRIQTKEPLPTLPLGRSADVRQGEFVVAMGSPFALQNTITSGIVSSAQRPARDLGLPQTNVEYIQTDAAIDFGNSGGPLVNLDGEVIGVNTMKVTAGISFAIPSDRLREFLHRGEKKNSSSGISGSQRRYIGVMMLTLSPSILAELQLREPSFPDVQHGVLIHKVILGSPAHRAGLRPGDVILAIGEQMVQNAEDVYEAVRTQSQLAVQIRRGRETLTLYVTPEVTE

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
89
Horse
89
Bovine
89
Sheep
89
Dog
89
Rabbit
89
Xenopus
0
Zebrafish
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - O43464 As Substrate

Site PTM Type Enzyme
R5 Methylation
R8 Methylation
T51 Phosphorylation
T54 Phosphorylation
S96 Phosphorylation
S142 Phosphorylation Q9BXM7 (PINK1) , P53778 (MAPK12)
T157 Phosphorylation
S212 Phosphorylation P31751 (AKT2) , P31749 (AKT1)
K237 Ubiquitination
T242 Phosphorylation
T326 Phosphorylation
S330 Phosphorylation
S352 Phosphorylation
K395 Ubiquitination
S400 Phosphorylation Q00535 (CDK5)
T453 Phosphorylation

Research Backgrounds

Function:

Serine protease that shows proteolytic activity against a non-specific substrate beta-casein. Promotes or induces cell death either by direct binding to and inhibition of BIRC proteins (also called inhibitor of apoptosis proteins, IAPs), leading to an increase in caspase activity, or by a BIRC inhibition-independent, caspase-independent and serine protease activity-dependent mechanism. Cleaves THAP5 and promotes its degradation during apoptosis. Isoform 2 seems to be proteolytically inactive.

PTMs:

Autoproteolytically activated.

Subcellular Location:

Mitochondrion intermembrane space. Mitochondrion membrane>Single-pass membrane protein.
Note: Predominantly present in the intermembrane space. Released into the cytosol following apoptotic stimuli, such as UV treatment, and stimulation of mitochondria with caspase-8 truncated BID/tBID.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Isoform 1 is ubiquitous. Isoform 2 is expressed predominantly in the kidney, colon and thyroid.

Subunit Structure:

Homotrimer. Interacts with MXI2. Interacts with THAP5 under apoptotic conditions. The mature protein, but not the precursor, binds to BIRC2/c-IAP1, BIRC3/c-IAP2 and XIAP/BIRC4. Interacts with BIRC6/bruce.

Family&Domains:

The mature N-terminus is involved in the interaction with XIAP.

The PDZ domain mediates interaction with MXI2.

Belongs to the peptidase S1C family.

Research Fields

· Cellular Processes > Cell growth and death > Apoptosis.   (View pathway)

· Cellular Processes > Cell growth and death > Apoptosis - multiple species.   (View pathway)

· Human Diseases > Neurodegenerative diseases > Parkinson's disease.

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