Phospho-BCL-3 (Ser402) Antibody | Affinity Biosciences

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Phospho-BCL-3 (Ser402) Antibody - AF8466 at 1/100 staining Human kidney cancer and adjacent normal tissues by IHC-P.

Phospho-BCL-3 (Ser402) Antibody - AF8466 at 1/100 staining Human kidney cancer by IHC-P.

Phospho-BCL-3 (Ser402) Antibody - AF8466 at 1/100 staining Human ovarian cancer by IHC-P.

Phospho-BCL-3 (Ser402) Antibody - peptide-ELISA analysis of AF8466.

Product:	Phospho-BCL-3 (Ser402) Antibody
Catalog:	AF8466
Description:	Rabbit polyclonal antibody to Phospho-BCL-3 (Ser402)
Application:	WB IHC
Reactivity:	Human
Prediction:	Horse
Mol.Wt.:	50-60kD; 48kD(Calculated).
Uniprot:	P20749
RRID:	AB_2840520

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$350	In stock
200ul	$450	In stock

Lead Time: Same day delivery

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Related Downloads

MS Report

HPLC Report

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:1000-3000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human

Prediction:

Horse(100%)

Clonality:

Polyclonal

Specificity:

Phospho-BCL-3 (Ser402) Antibody detects endogenous levels of BCL-3 only when phosphorylated at Ser402.

RRID:

AB_2840520
Cite Format: Affinity Biosciences Cat# AF8466, RRID:AB_2840520.

Conjugate:

Unconjugated.

Purification:

The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

B cell CLL/lymphoma 3; B cell leukemia/lymphoma 3; B cell lymphoma 3 encoded protein; B-cell lymphoma 3 protein; Bcl 3; BCL 4; BCL-3; Bcl3; BCL3_HUMAN; BCL4; Chronic lymphatic leukemia protein; D19S37; Protein Bcl 3; Proto-oncogene BCL3;

Immunogens

Immunogen:

A synthesized peptide derived from human BCL-3 around the phosphorylation site of Ser402.

Uniprot:

P20749(BCL3_HUMAN) >>Visit HPA database.

Gene(ID):

BCL3(602)

Sequence:

P20749 BCL3_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MPRCPAGAMDEGPVDLRTRPKAAGLPGAALPLRKRPLRAPSPEPAAPRGAAGLVVPLDPLRGGCDLPAVPGPPHGLARPEALYYPGALLPLYPTRAMGSPFPLVNLPTPLYPMMCPMEHPLSADIAMATRADEDGDTPLHIAVVQGNLPAVHRLVNLFQQGGRELDIYNNLRQTPLHLAVITTLPSVVRLLVTAGASPMALDRHGQTAAHLACEHRSPTCLRALLDSAAPGTLDLEARNYDGLTALHVAVNTECQETVQLLLERGADIDAVDIKSGRSPLIHAVENNSLSMVQLLLQHGANVNAQMYSGSSALHSASGRGLLPLVRTLVRSGADSSLKNCHNDTPLMVARSRRVIDILRGKATRPASTSQPDPSPDRSANTSPESSSRLSSNGLLSASPSSSPSQSPPRDPPGFPMAPPNFFLPSPSPPAFLPFAGVLRGPGRPVPPSPAPGGS

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Horse

100

Pig

Bovine

Sheep

Dog

Xenopus

Zebrafish

Chicken

Rabbit

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

Research Backgrounds

P20749_BCL3_HUMAN

Function:

Contributes to the regulation of transcriptional activation of NF-kappa-B target genes. In the cytoplasm, inhibits the nuclear translocation of the NF-kappa-B p50 subunit. In the nucleus, acts as transcriptional activator that promotes transcription of NF-kappa-B target genes. Contributes to the regulation of cell proliferation (By similarity).

PTMs:

Polyubiquitinated. Ubiquitination via 'Lys-63'-linked ubiquitin chains is required for nuclear accumulation. Deubiquitinated by CYLD, which acts on 'Lys-63'-linked ubiquitin chains. Deubiquitination by CYLD prevents nuclear accumulation (By similarity).

Activated by phosphorylation.

Subcellular Location:

Nucleus. Cytoplasm. Cytoplasm>Perinuclear region.
Note: Ubiquitination via 'Lys-63'-linked ubiquitin chains is required for nuclear accumulation.

Research Fields

· Environmental Information Processing > Signal transduction > TNF signaling pathway. (View pathway)

Restrictive clause

Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

For Research Use Only.
Not for use in diagnostic or therapeutic procedures. Not for resale. Not for distribution without written consent. Affinity Biosciences will not be held responsible for patent infringement or other violations that may occur with the use of our products. Affinity Biosciences, Affinity Biosciences Logo and all other trademarks are the property of Affinity Biosciences LTD.

Phospho-BCL-3 (Ser402) Antibody - #AF8466