ADNP Antibody | Affinity Biosciences

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Western blot analysis of extracts from Hybridoma cells, using ADNP Antibody.

Product:	ADNP Antibody
Catalog:	AF0418
Description:	Rabbit polyclonal antibody to ADNP
Application:	WB IF/ICC
Reactivity:	Human, Mouse
Prediction:	Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken
Mol.Wt.:	124kDa; 124kD(Calculated).
Uniprot:	Q9H2P0
RRID:	AB_2834336

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$280	In stock
200ul	$350	In stock

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Related Downloads

MS Report

HPLC Report

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:500-1:2000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse

Prediction:

Pig(92%), Bovine(83%), Horse(92%), Sheep(83%), Rabbit(100%), Dog(92%), Chicken(83%)

Clonality:

Polyclonal

Specificity:

ADNP Antibody detects endogenous levels of total ADNP.

RRID:

AB_2834336
Cite Format: Affinity Biosciences Cat# AF0418, RRID:AB_2834336.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

Activity dependent neuroprotective protein; Activity dependent neuroprotector; Activity-dependent neuroprotective protein; Activity-dependent neuroprotector homeobox protein; Adnp; ADNP_HUMAN; KIAA0784;

Immunogens

Immunogen:

Uniprot:

Q9H2P0(ADNP_HUMAN) >>Visit HPA database.

Gene(ID):

ADNP(23394)

Expression:

Q9H2P0 ADNP_HUMAN:

Widely expressed. Strong expression in heart, skeletal muscle, kidney and placenta. In brain, expression is stronger in the cerebellum and cortex regions. No expression detected in the colon. Strong increase of expression in colon and breast cancer tissues.

Description:

ADNP Potential transcription factor. May mediate some of the neuroprotective peptide VIP-associated effects involving normal growth and cancer proliferation.

Sequence:

Q9H2P0 ADNP_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MFQLPVNNLGSLRKARKTVKKILSDIGLEYCKEHIEDFKQFEPNDFYLKNTTWEDVGLWDPSLTKNQDYRTKPFCCSACPFSSKFFSAYKSHFRNVHSEDFENRILLNCPYCTFNADKKTLETHIKIFHAPNASAPSSSLSTFKDKNKNDGLKPKQADSVEQAVYYCKKCTYRDPLYEIVRKHIYREHFQHVAAPYIAKAGEKSLNGAVPLGSNAREESSIHCKRCLFMPKSYEALVQHVIEDHERIGYQVTAMIGHTNVVVPRSKPLMLIAPKPQDKKSMGLPPRIGSLASGNVRSLPSQQMVNRLSIPKPNLNSTGVNMMSSVHLQQNNYGVKSVGQGYSVGQSMRLGLGGNAPVSIPQQSQSVKQLLPSGNGRSYGLGSEQRSQAPARYSLQSANASSLSSGQLKSPSLSQSQASRVLGQSSSKPAAAATGPPPGNTSSTQKWKICTICNELFPENVYSVHFEKEHKAEKVPAVANYIMKIHNFTSKCLYCNRYLPTDTLLNHMLIHGLSCPYCRSTFNDVEKMAAHMRMVHIDEEMGPKTDSTLSFDLTLQQGSHTNIHLLVTTYNLRDAPAESVAYHAQNNPPVPPKPQPKVQEKADIPVKSSPQAAVPYKKDVGKTLCPLCFSILKGPISDALAHHLRERHQVIQTVHPVEKKLTYKCIHCLGVYTSNMTASTITLHLVHCRGVGKTQNGQDKTNAPSRLNQSPSLAPVKRTYEQMEFPLLKKRKLDDDSDSPSFFEEKPEEPVVLALDPKGHEDDSYEARKSFLTKYFNKQPYPTRREIEKLAASLWLWKSDIASHFSNKRKKCVRDCEKYKPGVLLGFNMKELNKVKHEMDFDAEWLFENHDEKDSRVNASKTADKKLNLGKEDDSSSDSFENLEEESNESGSPFDPVFEVEPKISNDNPEEHVLKVIPEDASESEEKLDQKEDGSKYETIHLTEEPTKLMHNASDSEVDQDDVVEWKDGASPSESGPGSQQVSDFEDNTCEMKPGTWSDESSQSEDARSSKPAAKKKATMQGDREQLKWKNSSYGKVEGFWSKDQSQWKNASENDERLSNPQIEWQNSTIDSEDGEQFDNMTDGVAEPMHGSLAGVKLSSQQA

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Rabbit

100

Pig

Horse

Dog

Bovine

Sheep

Chicken

Xenopus

Zebrafish

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q9H2P0 As Substrate

Q9H2P0

Site	PTM Type	Source
S11	Phosphorylation	Uniprot
K32	Ubiquitination	Uniprot
K39	Ubiquitination	Uniprot
K65	Ubiquitination	Uniprot
S87	Phosphorylation	Uniprot
S98	Phosphorylation	Uniprot
K126	Ubiquitination	Uniprot
K144	Sumoylation	Uniprot
K144	Ubiquitination	Uniprot
S213	Phosphorylation	Uniprot
T258	Phosphorylation	Uniprot
K274	Sumoylation	Uniprot
K279	Sumoylation	Uniprot
S289	Phosphorylation	Uniprot
S292	Phosphorylation	Uniprot
R306	Methylation	Uniprot
S336	Phosphorylation	Uniprot
S346	Phosphorylation	Uniprot
R348	Methylation	Uniprot
S372	Phosphorylation	Uniprot
R385	Methylation	Uniprot
R391	Methylation	Uniprot
S393	Phosphorylation	Uniprot
K408	Methylation	Uniprot
K408	Sumoylation	Uniprot
S409	Phosphorylation	Uniprot
S411	Phosphorylation	Uniprot
S413	Phosphorylation	Uniprot
S415	Phosphorylation	Uniprot
S418	Phosphorylation	Uniprot
S424	Phosphorylation	Uniprot
S425	Phosphorylation	Uniprot
K427	Sumoylation	Uniprot
K427	Ubiquitination	Uniprot
K445	Ubiquitination	Uniprot
K600	Sumoylation	Uniprot
K606	Sumoylation	Uniprot
S607	Phosphorylation	Uniprot
S608	Phosphorylation	Uniprot
Y615	Phosphorylation	Uniprot
K616	Ubiquitination	Uniprot
K621	Ubiquitination	Uniprot
K658	Sumoylation	Uniprot
S678	Phosphorylation	Uniprot
K692	Ubiquitination	Uniprot
S709	Phosphorylation	Uniprot
K716	Methylation	Uniprot
K716	Sumoylation	Uniprot
K716	Ubiquitination	Uniprot
Y719	Phosphorylation	Uniprot
K731	Sumoylation	Uniprot
K731	Ubiquitination	Uniprot
S736	Phosphorylation	Uniprot
S738	Phosphorylation	Uniprot
S740	Phosphorylation	Uniprot
K745	Sumoylation	Uniprot
K757	Ubiquitination	Uniprot
K768	Ubiquitination	Uniprot
S769	Phosphorylation	Uniprot
T772	Phosphorylation	Uniprot
K773	Ubiquitination	Uniprot
Y774	Phosphorylation	Uniprot
K777	Ubiquitination	Uniprot
S805	Phosphorylation	Uniprot
K807	Ubiquitination	Uniprot
K819	Sumoylation	Uniprot
K835	Sumoylation	Uniprot
T861	Phosphorylation	Uniprot
S875	Phosphorylation	Uniprot
S876	Phosphorylation	Uniprot
S878	Phosphorylation	Uniprot
S904	Phosphorylation	Uniprot
K914	Ubiquitination	Uniprot
S921	Phosphorylation	Uniprot
S923	Phosphorylation	Uniprot
K930	Sumoylation	Uniprot
K935	Ubiquitination	Uniprot
S953	Phosphorylation	Uniprot
S955	Phosphorylation	Uniprot
S970	Phosphorylation	Uniprot
S982	Phosphorylation	Uniprot
S997	Phosphorylation	Uniprot
S1000	Phosphorylation	Uniprot
S1001	Phosphorylation	Uniprot
S1003	Phosphorylation	Uniprot
S1032	Phosphorylation	Uniprot
K1035	Acetylation	Uniprot
K1035	Sumoylation	Uniprot
K1042	Acetylation	Uniprot
K1042	Ubiquitination	Uniprot
S1058	Phosphorylation	Uniprot
S1067	Phosphorylation	Uniprot
S1071	Phosphorylation	Uniprot
S1091	Phosphorylation	Uniprot

Research Backgrounds

Q9H2P0_ADNP_HUMAN

Function:

Potential transcription factor. May mediate some of the neuroprotective peptide VIP-associated effects involving normal growth and cancer proliferation.

Subcellular Location:

Nucleus.

Tissue Specificity:

Restrictive clause

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ADNP Antibody - #AF0418