ZNF295 Antibody - #DF10017

(1)  
Product: ZNF295 Antibody
Catalog: DF10017
Description: Rabbit polyclonal antibody to ZNF295
Application: WB IHC
Reactivity: Human, Mouse
Prediction: Pig, Bovine, Horse, Rabbit, Chicken
Mol.Wt.: 119 kDa; 119kD(Calculated).
Uniprot: Q9ULJ3
RRID: AB_2840597

View similar products>>

   Size Price Inventory
 100ul $280 In stock
 200ul $350 In stock

Lead Time: Same day delivery

For pricing and ordering contact:
Local distributors
Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:1000-3000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse
Prediction:
Pig(100%), Bovine(100%), Horse(91%), Rabbit(89%), Chicken(82%)
Clonality:
Polyclonal
Specificity:
ZNF295 Antibody detects endogenous levels of total ZNF295.
RRID:
AB_2840597
Cite Format: Affinity Biosciences Cat# DF10017, RRID:AB_2840597.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

Gene similar to zinc finger 5 protein; ZBT21_HUMAN; ZBTB21; Zfp295; Zinc finger and BTB domain containing 21; Zinc finger and BTB domain containing protein 21; Zinc finger and BTB domain-containing protein 21; Zinc finger protein 295; ZNF295;

Immunogens

Immunogen:
Uniprot:

Q9ULJ3(ZBT21_HUMAN) >>Visit HPA database.

Gene(ID):
ZBTB21(49854)
Expression:
Q9ULJ3 ZBT21_HUMAN:

Ubiquitous in fetal and adult tissues.

Sequence:
MEGLLHYINPAHAISLLSALNEERLKGQLCDVLLIVGDQKFRAHKNVLAASSEYFQSLFTNKENESQTVFQLDFCEPDAFDNVLNYIYSSSLFVEKSSLAAVQELGYSLGISFLTNIVSKTPQAPFPTCPNRKKVFVEDDENSSQKRSVIVCQSRNEAQGKTVSQNQPDVSHTSRPSPSIAVKANTNKPHVPKPIEPLHNLSLTEKSWPKDSSVVYAKSLEHSGSLDDPNRISLVKRNAVLPSKPLQDREAMDDKPGVSGQLPKGKALELALKRPRPPVLSVCSSSETPYLLKETNKGNGQGEDRNLLYYSKLGLVIPSSGSGSGNQSIDRSGPLVKSLLRRSLSMDSQVPVYSPSIDLKSSQGSSSVSSDAPGNVLCALSQKSSLKDCSEKTALDDRPQVLQPHRLRSFSASQSTDREGASPVTEVRIKTEPSSPLSDPSDIIRVTVGDAATTAAASSSSVTRDLSLKTEDDQKDMSRLPAKRRFQADRRLPFKKLKVNEHGSPVSEDNFEEGSSPTLLDADFPDSDLNKDEFGELEGTRPNKKFKCKHCLKIFRSTAGLHRHVNMYHNPEKPYACDICHKRFHTNFKVWTHCQTQHGIVKNPSPASSSHAVLDEKFQRKLIDIVREREIKKALIIKLRRGKPGFQGQSSSQAQQVIKRNLRSRAKGAYICTYCGKAYRFLSQFKQHIKMHPGEKPLGVNKVAKPKEHAPLASPVENKEVYQCRLCNAKLSSLLEQGSHERLCRNAAVCPYCSLRFFSPELKQEHESKCEYKKLTCLECMRTFKSSFSIWRHQVEVHNQNNMAPTENFSLPVLDHNGDVTGSSRPQSQPEPNKVNHIVTTKDDNVFSDSSEQVNFDSEDSSCLPEDLSLSKQLKIQVKEEPVEEAEEEAPEASTAPKEAGPSKEASLWPCEKCGKMFTVHKQLERHQELLCSVKPFICHVCNKAFRTNFRLWSHFQSHMSQASEESAHKESEVCPVPTNSPSPPPLPPPPPLPKIQPLEPDSPTGLSENPTPATEKLFVPQESDTLFYHAPPLSAITFKRQFMCKLCHRTFKTAFSLWSHEQTHN

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Bovine
100
Horse
91
Rabbit
89
Chicken
82
Sheep
0
Dog
0
Xenopus
0
Zebrafish
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q9ULJ3 As Substrate

Site PTM Type Enzyme
K40 Sumoylation
S177 Phosphorylation
S202 Phosphorylation
S219 Phosphorylation
S225 Phosphorylation
S233 Phosphorylation
K244 Acetylation
K273 Acetylation
S281 Phosphorylation
Y309 Phosphorylation
S328 Phosphorylation
S332 Phosphorylation
S343 Phosphorylation
S345 Phosphorylation
S348 Phosphorylation
Y353 Phosphorylation
S354 Phosphorylation
S361 Phosphorylation
S362 Phosphorylation
S365 Phosphorylation
S369 Phosphorylation
S370 Phosphorylation
S381 Phosphorylation
R398 Methylation
S409 Phosphorylation
S411 Phosphorylation
S413 Phosphorylation
T416 Phosphorylation
S422 Phosphorylation
T425 Phosphorylation
K430 Sumoylation
T431 Phosphorylation
S434 Phosphorylation
S435 Phosphorylation
S438 Phosphorylation
S441 Phosphorylation
T453 Phosphorylation
S467 Phosphorylation
K469 Sumoylation
S478 Phosphorylation
K495 Acetylation
S504 Phosphorylation
S507 Phosphorylation
S515 Phosphorylation
K602 Ubiquitination
S605 Phosphorylation
S608 Phosphorylation
K617 Sumoylation
K617 Ubiquitination
K643 Ubiquitination
S714 Phosphorylation
S739 Phosphorylation
S759 Phosphorylation
K879 Sumoylation
S972 Phosphorylation
T979 Phosphorylation
S981 Phosphorylation
S983 Phosphorylation
S1003 Phosphorylation
T1005 Phosphorylation
T1038 Phosphorylation
K1040 Acetylation

Research Backgrounds

Function:

Acts as a transcription repressor.

Subcellular Location:

Nucleus.
Note: Colocalizes with ZBTB14 in nucleus in HEK293 cells.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Ubiquitous in fetal and adult tissues.

Subunit Structure:

Homodimer. Interacts with ZBTB14.

Restrictive clause

 

Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

For Research Use Only.
Not for use in diagnostic or therapeutic procedures. Not for resale. Not for distribution without written consent. Affinity Biosciences will not be held responsible for patent infringement or other violations that may occur with the use of our products. Affinity Biosciences, Affinity Biosciences Logo and all other trademarks are the property of Affinity Biosciences LTD.