ZP2 Antibody - #DF10022

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Product: ZP2 Antibody
Catalog: DF10022
Description: Rabbit polyclonal antibody to ZP2
Application: WB IHC
Reactivity: Human, Monkey
Prediction: Horse
Mol.Wt.: 82 kDa; 82kD(Calculated).
Uniprot: Q05996
RRID: AB_2840602

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:1000-3000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Monkey
Prediction:
Horse(89%)
Clonality:
Polyclonal
Specificity:
ZP2 Antibody detects endogenous levels of total ZP2.
RRID:
AB_2840602
Cite Format: Affinity Biosciences Cat# DF10022, RRID:AB_2840602.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

OTTHUMP00000115849; Processed zona pellucida sperm-binding protein 2; Zona pellucida glycoprotein 2; zona pellucida glycoprotein ZP2; Zona pellucida protein A; Zp-2; ZP2; ZP2_HUMAN; ZPA;

Immunogens

Immunogen:
Uniprot:

Q05996(ZP2_HUMAN) >>Visit HPA database.

Gene(ID):
ZP2(7783)
Expression:
Q05996 ZP2_HUMAN:

Expressed in occytes(at protein level).

Sequence:
MACRQRGGSWSPSGWFNAGWSTYRSISLFFALVTSGNSIDVSQLVNPAFPGTVTCDEREITVEFPSSPGTKKWHASVVDPLGLDMPNCTYILDPEKLTLRATYDNCTRRVHGGHQMTIRVMNNSAALRHGAVMYQFFCPAMQVEETQGLSASTICQKDFMSFSLPRVFSGLADDSKGTKVQMGWSIEVGDGARAKTLTLPEAMKEGFSLLIDNHRMTFHVPFNATGVTHYVQGNSHLYMVSLKLTFISPGQKVIFSSQAICAPDPVTCNATHMTLTIPEFPGKLKSVSFENQNIDVSQLHDNGIDLEATNGMKLHFSKTLLKTKLSEKCLLHQFYLASLKLTFLLRPETVSMVIYPECLCESPVSIVTGELCTQDGFMDVEVYSYQTQPALDLGTLRVGNSSCQPVFEAQSQGLVRFHIPLNGCGTRYKFEDDKVVYENEIHALWTDFPPSKISRDSEFRMTVKCSYSRNDMLLNINVESLTPPVASVKLGPFTLILQSYPDNSYQQPYGENEYPLVRFLRQPIYMEVRVLNRDDPNIKLVLDDCWATSTMDPDSFPQWNVVVDGCAYDLDNYQTTFHPVGSSVTHPDHYQRFDMKAFAFVSEAHVLSSLVYFHCSALICNRLSPDSPLCSVTCPVSSRHRRATGATEAEKMTVSLPGPILLLSDDSSFRGVGSSDLKASGSSGEKSRSETGEEVGSRGAMDTKGHKTAGDVGSKAVAAVAAFAGVVATLGFIYYLYEKRTVSNH

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Horse
89
Bovine
67
Sheep
64
Dog
64
Rabbit
64
Pig
0
Xenopus
0
Zebrafish
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q05996 As Substrate

Site PTM Type Enzyme
K96 Acetylation
T178 Phosphorylation
K318 Acetylation
K322 Acetylation
S689 Phosphorylation
S697 Phosphorylation
T703 Phosphorylation

Research Backgrounds

Function:

Component of the zona pellucida, an extracellular matrix surrounding oocytes which mediates sperm binding, induction of the acrosome reaction and prevents post-fertilization polyspermy. The zona pellucida is composed of 3 to 4 glycoproteins, ZP1, ZP2, ZP3, and ZP4. ZP2 may act as a secondary sperm receptor.

PTMs:

Proteolytically cleaved before the transmembrane segment to yield the secreted ectodomain incorporated in the zona pellucida.

Proteolytically cleaved in the N-terminal part by the metalloendopeptidase ASTL exocytosed from cortical granules after fertilization, yielding a N-terminal peptide of about 30 kDa which remains covalently attached to the C-terminal peptide via disulfide bond(s). This cleavage may play an important role in the post-fertilization block to polyspermy. Additional proteolytically cleavage of the N-terminal peptide of 30 kDa occurs in one-cell and two-cell embryos.

N-glycosylated.

O-glycosylated; contains sulfate-substituted glycans.

Subcellular Location:

Secreted>Extracellular space>Extracellular matrix.
Note: Located in oocyte zona pellucida.

Cell membrane>Single-pass type I membrane protein.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Expressed in occytes(at protein level).

Subunit Structure:

Can form homopolymers that assemble into long fibers (in vitro). Polymers of ZP2 and ZP3 organized into long filaments cross-linked by ZP1 homodimers (By similarity). Interacts with ZP3.

Family&Domains:

The ZP domain is involved in the polymerization of the ZP proteins to form the zona pellucida.

Belongs to the ZP domain family. ZPA subfamily.

References

1). Maternal ochratoxin A exposure impairs meiosis progression and primordial follicle formation of F1 offspring. FOOD AND CHEMICAL TOXICOLOGY, 2022 (PubMed: 36007852) [IF=3.9]

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