CD2L7 Antibody - #DF10028

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Product: CD2L7 Antibody
Catalog: DF10028
Description: Rabbit polyclonal antibody to CD2L7
Application: WB
Reactivity: Human, Rat
Prediction: Pig, Bovine, Horse, Sheep, Rabbit, Dog
Mol.Wt.: 164 kDa; 164kD(Calculated).
Uniprot: Q9NYV4
RRID: AB_2840608

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 100ul $280 In stock
 200ul $350 In stock

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Related Downloads
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:1000-3000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Rat
Prediction:
Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%)
Clonality:
Polyclonal
Specificity:
CD2L7 Antibody detects endogenous levels of total CD2L7.
RRID:
AB_2840608
Cite Format: Affinity Biosciences Cat# DF10028, RRID:AB_2840608.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

arginine/serine-rich; Cdc2 related kinase arginine/serine rich; CDC2 related protein kinase 7; Cdc2-related kinase; CDC2-related protein kinase 7; Cdk12; CDK12_HUMAN; Cell division cycle 2 related protein kinase 7; Cell division cycle 2-related protein kinase 7; Cell division protein kinase 12; CRK7; CRKR; CrkRS; Cyclin-dependent kinase 12; EC 2.7.11.22; hCDK12; kiaa0904; Pksc;

Immunogens

Immunogen:
Uniprot:

Q9NYV4(CDK12_HUMAN) >>Visit HPA database.

Gene(ID):
CDK12(51755)
Expression:
Q9NYV4 CDK12_HUMAN:

Ubiquitously expressed.

Sequence:
MPNSERHGGKKDGSGGASGTLQPSSGGGSSNSRERHRLVSKHKRHKSKHSKDMGLVTPEAASLGTVIKPLVEYDDISSDSDTFSDDMAFKLDRRENDERRGSDRSDRLHKHRHHQHRRSRDLLKAKQTEKEKSQEVSSKSGSMKDRISGSSKRSNEETDDYGKAQVAKSSSKESRSSKLHKEKTRKERELKSGHKDRSKSHRKRETPKSYKTVDSPKRRSRSPHRKWSDSSKQDDSPSGASYGQDYDLSPSRSHTSSNYDSYKKSPGSTSRRQSVSPPYKEPSAYQSSTRSPSPYSRRQRSVSPYSRRRSSSYERSGSYSGRSPSPYGRRRSSSPFLSKRSLSRSPLPSRKSMKSRSRSPAYSRHSSSHSKKKRSSSRSRHSSISPVRLPLNSSLGAELSRKKKERAAAAAAAKMDGKESKGSPVFLPRKENSSVEAKDSGLESKKLPRSVKLEKSAPDTELVNVTHLNTEVKNSSDTGKVKLDENSEKHLVKDLKAQGTRDSKPIALKEEIVTPKETETSEKETPPPLPTIASPPPPLPTTTPPPQTPPLPPLPPIPALPQQPPLPPSQPAFSQVPASSTSTLPPSTHSKTSAVSSQANSQPPVQVSVKTQVSVTAAIPHLKTSTLPPLPLPPLLPGDDDMDSPKETLPSKPVKKEKEQRTRHLLTDLPLPPELPGGDLSPPDSPEPKAITPPQQPYKKRPKICCPRYGERRQTESDWGKRCVDKFDIIGIIGEGTYGQVYKAKDKDTGELVALKKVRLDNEKEGFPITAIREIKILRQLIHRSVVNMKEIVTDKQDALDFKKDKGAFYLVFEYMDHDLMGLLESGLVHFSEDHIKSFMKQLMEGLEYCHKKNFLHRDIKCSNILLNNSGQIKLADFGLARLYNSEESRPYTNKVITLWYRPPELLLGEERYTPAIDVWSCGCILGELFTKKPIFQANLELAQLELISRLCGSPCPAVWPDVIKLPYFNTMKPKKQYRRRLREEFSFIPSAALDLLDHMLTLDPSKRCTAEQTLQSDFLKDVELSKMAPPDLPHWQDCHELWSKKRRRQRQSGVVVEEPPPSKTSRKETTSGTSTEPVKNSSPAPPQPAPGKVESGAGDAIGLADITQQLNQSELAVLLNLLQSQTDLSIPQMAQLLNIHSNPEMQQQLEALNQSISALTEATSQQQDSETMAPEESLKEAPSAPVILPSAEQTTLEASSTPADMQNILAVLLSQLMKTQEPAGSLEENNSDKNSGPQGPRRTPTMPQEEAAACPPHILPPEKRPPEPPGPPPPPPPPPLVEGDLSSAPQELNPAVTAALLQLLSQPEAEPPGHLPHEHQALRPMEYSTRPRPNRTYGNTDGPETGFSAIDTDERNSGPALTESLVQTLVKNRTFSGSLSHLGESSSYQGTGSVQFPGDQDLRFARVPLALHPVVGQPFLKAEGSSNSVVHAETKLQNYGELGPGTTGASSSGAGLHWGGPTQSSAYGKLYRGPTRVPPRGGRGRGVPY

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Dog
100
Rabbit
100
Chicken
57
Xenopus
0
Zebrafish
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q9NYV4 As Substrate

Site PTM Type Enzyme
S14 Phosphorylation
S18 Phosphorylation
S24 Phosphorylation
S25 Phosphorylation
S29 Phosphorylation
S30 Phosphorylation
S32 Phosphorylation
T57 Phosphorylation
Y73 Phosphorylation
S77 Phosphorylation
S78 Phosphorylation
S80 Phosphorylation
T82 Phosphorylation
S84 Phosphorylation
S148 Phosphorylation
S154 Phosphorylation
T158 Phosphorylation
Y161 Phosphorylation
S176 Phosphorylation
S177 Phosphorylation
K203 Acetylation
T206 Phosphorylation
K211 Acetylation
S215 Phosphorylation
S222 Phosphorylation
S228 Phosphorylation
S230 Phosphorylation
S231 Phosphorylation
S236 Phosphorylation
S238 Phosphorylation
S241 Phosphorylation
S249 Phosphorylation
S251 Phosphorylation
S253 Phosphorylation
T255 Phosphorylation
S256 Phosphorylation
S257 Phosphorylation
S261 Phosphorylation
S265 Phosphorylation
S274 Phosphorylation
S276 Phosphorylation
Y279 Phosphorylation
S283 Phosphorylation
Y285 Phosphorylation
S287 Phosphorylation
S288 Phosphorylation
S291 Phosphorylation
S293 Phosphorylation
S301 Phosphorylation
S303 Phosphorylation
Y305 Phosphorylation
S306 Phosphorylation
S310 Phosphorylation
S311 Phosphorylation
S312 Phosphorylation
Y313 Phosphorylation
S316 Phosphorylation
S318 Phosphorylation
Y319 Phosphorylation
S320 Phosphorylation
S323 Phosphorylation
S325 Phosphorylation
Y327 Phosphorylation
R329 Methylation
R330 Methylation
R331 Methylation
S332 Phosphorylation
S333 Phosphorylation
S334 Phosphorylation
S338 Phosphorylation
S341 Phosphorylation
S343 Phosphorylation
S345 Phosphorylation
S349 Phosphorylation
S355 Phosphorylation
S357 Phosphorylation
S359 Phosphorylation
Y362 Phosphorylation
S363 Phosphorylation
S382 Phosphorylation
S383 Phosphorylation
S385 Phosphorylation
S393 Phosphorylation
S394 Phosphorylation
S400 Phosphorylation
S420 Phosphorylation
K421 Ubiquitination
S423 Phosphorylation
S434 Phosphorylation
S440 Phosphorylation
R449 Methylation
S503 Phosphorylation
K504 Acetylation
K509 Acetylation
T514 Phosphorylation
T520 Phosphorylation
S521 Phosphorylation
T592 O-Glycosylation
S597 O-Glycosylation
S614 Phosphorylation
S644 Phosphorylation
S681 Phosphorylation
S685 Phosphorylation
T692 Phosphorylation
K747 Ubiquitination
K756 Ubiquitination
K764 Ubiquitination
T770 Phosphorylation
K796 Acetylation
K796 Ubiquitination
K803 Ubiquitination
S838 Phosphorylation
Y849 Phosphorylation
K861 Ubiquitination
S886 Phosphorylation
S889 Phosphorylation
Y892 Phosphorylation
T893 Phosphorylation P50613 (CDK7)
S954 Phosphorylation
K973 Acetylation
T1002 Phosphorylation
S1006 Phosphorylation
K1021 Ubiquitination
K1027 Ubiquitination
K1045 Ubiquitination
S1053 Phosphorylation
S1063 Phosphorylation
S1066 Phosphorylation
T1070 Phosphorylation
T1071 Phosphorylation
S1075 Phosphorylation
T1076 Phosphorylation
S1082 Phosphorylation
S1083 Phosphorylation
S1226 Phosphorylation
S1232 Phosphorylation
T1244 Phosphorylation
T1246 Phosphorylation
T1375 Phosphorylation
S1387 Phosphorylation
R1404 Methylation
K1470 Acetylation
R1473 Methylation

PTMs - Q9NYV4 As Enzyme

Substrate Site Source
P24928 (POLR2A) S1616 Uniprot
P24928 (POLR2A) S1619 Uniprot
P24928 (POLR2A) S1861 Uniprot
P24928 (POLR2A) S1868 Uniprot
P24928 (POLR2A) S1875 Uniprot
P24928 (POLR2A) S1878 Uniprot
P24928 (POLR2A) S1882 Uniprot
P24928 (POLR2A) S1889 Uniprot
P24928 (POLR2A) S1896 Uniprot
P24928 (POLR2A) S1910 Uniprot
P24928 (POLR2A) S1917 Uniprot
P24928 (POLR2A) S1924 Uniprot
P24928 (POLR2A) S1931 Uniprot
P24928 (POLR2A) S1941 Uniprot
P24928 (POLR2A) S1948 Uniprot
Q9NYV4-1 (CDK12) S274 Uniprot
Q9NYV4-1 (CDK12) S276 Uniprot
Q9NYV4-1 (CDK12) S383 Uniprot
Q9NYV4-1 (CDK12) S385 Uniprot
Q9NYV4-1 (CDK12) S681 Uniprot
Q9NYV4-1 (CDK12) S685 Uniprot
Q9NYV4-1 (CDK12) T893 Uniprot

Research Backgrounds

Function:

Cyclin-dependent kinase that phosphorylates the C-terminal domain (CTD) of the large subunit of RNA polymerase II (POLR2A), thereby acting as a key regulator of transcription elongation. Regulates the expression of genes involved in DNA repair and is required for the maintenance of genomic stability. Preferentially phosphorylates 'Ser-5' in CTD repeats that are already phosphorylated at 'Ser-7', but can also phosphorylate 'Ser-2'. Required for RNA splicing, possibly by phosphorylating SRSF1/SF2. Involved in regulation of MAP kinase activity, possibly leading to affect the response to estrogen inhibitors.

PTMs:

Phosphorylation at Thr-893 increases kinase activity.

Subcellular Location:

Nucleus. Nucleus speckle.
Note: Colocalized with nuclear speckles throughout interphase.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Ubiquitously expressed.

Subunit Structure:

Interacts with CCNL1 and CCNL2 (By similarity). Interacts with CCNK.

Family&Domains:

Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. CDC2/CDKX subfamily.

Restrictive clause

 

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